Standard name | |
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Human Ortholog | |
Description | Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 1 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 4 | 5 | 5 | 8 | 17 | 6 | 11 | 8 | 8 | 7 | 4 | 4 | 6 | 3 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 193 | 146 | 266 | 164 | 295 | 284 | 438 | 369 | 261 | 247 | 213 | 242 | 313 | 448 | 613 | 286 | 196 | 221 | 185 | 135 | 252 | 201 | 353 | 412 |
Endoplasmic Reticulum | 0 | 0 | 4 | 2 | 1 | 4 | 21 | 24 | 11 | 6 | 5 | 14 | 1 | 2 | 7 | 11 | 5 | 4 | 0 | 0 | 0 | 8 | 9 | 12 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 2 | 9 | 10 | 15 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 |
Nucleus | 0 | 0 | 1 | 2 | 3 | 3 | 6 | 7 | 3 | 6 | 6 | 2 | 3 | 1 | 4 | 2 | 1 | 0 | 0 | 4 | 2 | 0 | 2 | 6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 3 | 3 | 1 | 2 | 6 | 16 | 10 | 37 | 19 | 22 | 2 | 4 | 3 | 4 | 2 | 6 | 0 | 4 | 6 | 2 | 11 | 12 |
Unique Cell Count | 194 | 147 | 273 | 169 | 299 | 292 | 469 | 411 | 299 | 303 | 258 | 283 | 320 | 454 | 622 | 290 | 198 | 224 | 191 | 149 | 267 | 221 | 387 | 461 |
Labelled Cell Count | 194 | 149 | 278 | 177 | 305 | 303 | 493 | 425 | 312 | 318 | 271 | 294 | 327 | 461 | 636 | 308 | 205 | 237 | 191 | 149 | 267 | 221 | 387 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 33.8 | 27.4 | 25.1 | 20.9 | 21.6 | 18.7 | 17.9 | 17.0 | 14.6 | 13.3 | 13.1 | 13.9 | 26.6 | 27.4 | 27.2 | 42.5 | 39.4 | 41.4 | 25.7 | 26.9 | 29.1 |
Std Deviation (1e-4) | 6.9 | 7.0 | 5.4 | 5.4 | 4.1 | 4.2 | 4.1 | 3.7 | 3.6 | 3.4 | 3.9 | 3.6 | 6.2 | 6.3 | 6.1 | 12.4 | 11.4 | 9.9 | 5.2 | 6.1 | 7.0 |
Intensity Change (Log2) | – | – | – | -0.27 | -0.22 | -0.42 | -0.49 | -0.56 | -0.78 | -0.92 | -0.94 | -0.85 | 0.08 | 0.13 | 0.11 | 0.76 | 0.65 | 0.72 | 0.04 | 0.1 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.1 | 1.7 | 0 | 1.7 | 1.0 | 1.3 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | 1.1 | -0.1 | -2.4 | -3.8 | -4.5 | -6.1 | -5.8 | -5.0 | 0.3 | 1.2 | 1.2 | 1.0 | 1.2 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.2 | 2.8 | 1.7 | 0 | 0 | 2.3 | 0 | 0 | 0 | 1.7 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.2 | 1.8 | 5.2 | 3.6 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 16.2851 | 22.6154 | 19.8754 | 16.1759 | 19.0469 | 20.6804 | 18.7289 | 26.4922 | 23.0856 | 22.3716 | 20.9291 | 23.141 | 11.0618 | 19.6598 | 16.4805 | 19.6283 | 20.0081 | 18.7302 |
Actin | 0.0083 | 0.0003 | 0.0012 | 0.0631 | 0.0565 | 0.0005 | 0.0155 | 0.0001 | 0.0025 | 0.0011 | 0.004 | 0 | 0.0206 | 0.0098 | 0.0338 | 0.0001 | 0 | 0.0001 |
Bud | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0009 | 0.0013 | 0.0003 | 0.0011 | 0.0006 | 0 | 0.0026 | 0.0031 | 0.0035 | 0.0001 | 0 | 0 |
Bud Neck | 0.0007 | 0.0002 | 0.0005 | 0.001 | 0.0009 | 0.0014 | 0.0017 | 0.0001 | 0.0002 | 0.0003 | 0.0007 | 0.0003 | 0.0103 | 0.0013 | 0.0005 | 0.0002 | 0.0002 | 0.0003 |
Bud Periphery | 0.0005 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0 | 0.0017 | 0.003 | 0.0036 | 0 | 0 | 0 |
Bud Site | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.006 | 0.0004 | 0.001 | 0.0001 | 0.0053 | 0 | 0.0456 | 0.0181 | 0.0048 | 0.0004 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0006 | 0 | 0 | 0 |
Cytoplasm | 0.8807 | 0.9861 | 0.9404 | 0.9075 | 0.8666 | 0.9482 | 0.8958 | 0.9901 | 0.9292 | 0.9396 | 0.8475 | 0.9784 | 0.5839 | 0.8371 | 0.8049 | 0.8358 | 0.7467 | 0.9045 |
Cytoplasmic Foci | 0.0125 | 0.0003 | 0.0052 | 0.0031 | 0.0011 | 0.0013 | 0.0152 | 0.0012 | 0.0078 | 0.0008 | 0.0136 | 0.0003 | 0.0165 | 0.009 | 0.0041 | 0.0482 | 0.0001 | 0.0006 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0055 | 0.0005 | 0.0013 | 0.0008 | 0.0044 | 0.0019 | 0.0041 | 0.0002 | 0.0012 | 0.0003 | 0.0047 | 0.0004 | 0.0093 | 0.0003 | 0.0046 | 0.0004 | 0.0001 | 0.0003 |
Endosome | 0.0111 | 0.0001 | 0.0021 | 0.0007 | 0.0117 | 0.0014 | 0.0126 | 0.0002 | 0.0199 | 0.0029 | 0.0329 | 0.0001 | 0.0533 | 0.0003 | 0.002 | 0.0965 | 0 | 0.0002 |
Golgi | 0.0024 | 0 | 0.0003 | 0.0013 | 0.0053 | 0.0001 | 0.0084 | 0 | 0.0043 | 0.0045 | 0.0225 | 0 | 0.0142 | 0.0006 | 0.0043 | 0.0047 | 0 | 0 |
Lipid Particles | 0.006 | 0 | 0.0008 | 0.001 | 0.0013 | 0 | 0.0087 | 0 | 0.0039 | 0.0001 | 0.0252 | 0 | 0.0062 | 0.0001 | 0.0003 | 0.001 | 0 | 0 |
Mitochondria | 0.001 | 0 | 0.0002 | 0.0002 | 0.0019 | 0.0002 | 0.0016 | 0.0004 | 0.0065 | 0.0251 | 0.0195 | 0 | 0.0194 | 0.0001 | 0.0042 | 0.0004 | 0.0001 | 0 |
None | 0.0099 | 0.0002 | 0.0036 | 0.0006 | 0.0022 | 0.0004 | 0.0012 | 0.0003 | 0.0006 | 0.0005 | 0.0003 | 0.0002 | 0.0234 | 0.0188 | 0.0165 | 0.0004 | 0 | 0.0003 |
Nuclear Periphery | 0.0079 | 0.0016 | 0.0045 | 0.0016 | 0.0134 | 0.0051 | 0.0025 | 0.0004 | 0.0026 | 0.0014 | 0.0021 | 0.0023 | 0.0449 | 0.0003 | 0.0237 | 0.0007 | 0.0004 | 0.0017 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0004 | 0 | 0.0017 | 0.0004 | 0.0003 | 0 | 0.001 | 0.0002 |
Nucleus | 0.0409 | 0.0102 | 0.0328 | 0.0149 | 0.0303 | 0.0367 | 0.0144 | 0.0028 | 0.009 | 0.0207 | 0.0039 | 0.0175 | 0.1242 | 0.0951 | 0.0817 | 0.0054 | 0.2513 | 0.0913 |
Peroxisomes | 0.0027 | 0 | 0.0002 | 0.0025 | 0.0004 | 0 | 0.0061 | 0.0001 | 0.0012 | 0.0001 | 0.0116 | 0 | 0.0028 | 0.0009 | 0.0026 | 0.0023 | 0 | 0 |
Punctate Nuclear | 0.0041 | 0.0001 | 0.0052 | 0.0007 | 0.0012 | 0.0008 | 0.0014 | 0.0001 | 0.0009 | 0.0003 | 0.001 | 0.0001 | 0.0113 | 0.0003 | 0.0007 | 0.0007 | 0 | 0.0004 |
Vacuole | 0.0039 | 0.0001 | 0.0007 | 0.0003 | 0.0005 | 0.0012 | 0.0022 | 0.0011 | 0.0061 | 0.0004 | 0.0021 | 0.0002 | 0.0036 | 0.0008 | 0.001 | 0.0018 | 0 | 0.0001 |
Vacuole Periphery | 0.0009 | 0 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0021 | 0.0004 | 0.0013 | 0.0001 | 0.0043 | 0.0001 | 0.0022 | 0.0006 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.7701 | 95.9137 | 124.9782 | 135.4522 | 114.9052 | 63.4825 | 93.8749 | 100.7988 | 109.5372 | 103.4979 |
Translational Efficiency | 1.0292 | 1.1013 | 1.0199 | 1.0949 | 1.0102 | 1.0058 | 0.9412 | 0.8464 | 0.9328 | 0.8549 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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