Standard name
Human Ortholog
Description Essential protein that interacts with proteasome components; has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.12 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.32 0.28 0.14 0.08 0.05 0.15 0.18 0.14 0.2 0.17 0.34 0.16 0.11 0.05 0 0.12 0.07 0.08 0 0 0 0 0 0.06
Nucleus 0.24 0.28 0.12 0.06 0.14 0.06 0.09 0.1 0.1 0.12 0.11 0.06 0 0 0.06 0.08 0.11 0.12 0.15 0.16 0.07 0.06 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.79 0.78 0.82 0.93 0.81 0.86 0.82 0.82 0.83 0.81 0.81 0.85 0.94 0.85 0.81 0.77 0.86 0.87 0.73 0.75 0.76 0.77 0.67 0.67
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.11 0.08 0.13 0.12 0.11 0.12 0.13 0.07 0.11 0.11 0.07 0.09 0 0 0 0 0 0.05 0.06 0.07 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0
Bud 0 0 1 0 0 0 5 1 3 2 2 2 0 0 1 0 0 1 3 0 2 2 2 5
Bud Neck 1 0 0 4 2 1 2 2 1 1 0 3 6 7 3 0 0 0 0 0 0 2 3 4
Bud Site 1 0 0 1 1 2 9 6 22 13 9 8 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 5 4 12 10 17 13 10 5 6 6 15 48 61 3 13 8 0 0 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 1 0
Endosome 0 0 3 4 19 10 2 16 9 11 1 15 9 8 11 0 1 0 3 1 5 3 19 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 2 2 2
Mitochondria 145 58 41 27 19 57 84 67 104 68 133 63 52 24 22 3 18 21 6 1 4 4 24 31
Nucleus 108 57 34 21 51 22 42 49 53 48 43 24 18 19 35 2 27 31 73 35 23 22 33 27
Nuclear Periphery 2 1 2 0 5 0 3 1 5 10 7 3 0 0 2 0 0 0 7 1 6 1 6 7
Nucleolus 356 162 233 302 301 330 384 385 438 331 321 337 424 445 477 20 207 228 363 171 235 289 368 383
Peroxisomes 1 1 3 11 3 14 7 4 13 1 0 1 8 7 13 0 1 2 0 0 0 1 2 7
SpindlePole 2 0 8 35 30 51 55 52 64 54 28 43 51 39 54 0 1 0 8 2 16 21 36 32
Vac/Vac Membrane 10 3 5 0 7 3 6 1 4 3 1 6 1 3 3 0 3 5 24 9 10 16 40 40
Unique Cell Count 451 207 284 325 370 382 467 468 529 410 397 397 453 522 590 26 242 262 497 228 310 378 551 571
Labelled Cell Count 628 286 335 409 450 500 616 597 726 547 551 511 586 602 682 30 273 296 497 228 310 378 551 571


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.9 31.0 22.6 19.6 16.3 13.0 11.8 11.9 11.4 10.9 10.5 11.4 22.2 23.1 22.1 32.7 36.7 37.0 24.4 24.7 27.1
Std Deviation (1e-4) 6.2 10.4 7.6 5.9 4.3 3.8 3.6 3.8 3.5 3.5 4.4 3.6 8.9 11.3 11.6 14.3 15.8 16.7 9.5 9.0 9.5
Intensity Change (Log2) – – – -0.2 -0.47 -0.8 -0.94 -0.92 -0.98 -1.05 -1.1 -0.98 -0.02 0.04 -0.03 0.54 0.7 0.71 0.11 0.13 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.5 3.0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.2 0.7 1.5 0.9 0.1 0 0 0 1.3 4.1 4.5 0 2.3 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.9 1.4 0 2.0 0 1.5 0 2.2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.4 -4.1 0.2 1.3 0 1.9 0.8 5.6 0.5 -1.2 -4.9 -5.7 0 -2.5 -2.4
Nucleus -2.4 0.7 -2.9 -1.3 -0.6 -0.9 -0.1 -0.5 -2.7 -4.1 -4.6 -3.1 0 -0.3 -0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.1 -0.2 1.5 0.1 0.1 0.3 -0.4 -0.4 1.0 4.9 1.2 -0.4 -0.6 1.1 1.6
Peroxisomes 1.9 0 2.1 0 0 1.4 0 0 0 0 0 1.2 0 0 0
SpindlePole 3.8 2.9 4.7 4.3 4.1 4.4 4.7 2.4 3.9 4.1 2.7 3.4 0 0 0
Vacuole 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.0433 29.163 25.0328 25.0423 19.708 24.9639 21.1905 27.3984 23.5541 20.6288 16.9789 22.8218 25.2838 31.5521 26.0728 24.4882 24.9543 27.9769
Actin 0.0188 0 0.0146 0 0.0068 0.0002 0.0008 0 0 0.0266 0 0.0035 0.0022 0 0.0001 0.0131 0.0219 0.0018
Bud 0.0002 0.0001 0.0003 0.0001 0.0002 0 0.0002 0 0 0.0003 0 0.0001 0.0001 0 0 0.0001 0.0005 0.0001
Bud Neck 0.0004 0.0001 0.0004 0.0003 0.0003 0.0001 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0.0002 0.0002 0.0001
Bud Periphery 0.0003 0.0003 0.0004 0.0003 0.0002 0.0001 0.0003 0 0 0.0005 0 0.0002 0.0001 0 0 0.0001 0.001 0.0002
Bud Site 0.0007 0 0.0037 0.0001 0.0007 0 0.0019 0 0.0001 0.0018 0.0002 0.0001 0.0003 0 0.0001 0.0004 0.001 0.0001
Cell Periphery 0.0013 0.0007 0.0002 0.0009 0.0002 0.0006 0.0003 0.0003 0.0005 0.0002 0.0007 0.0004 0.0003 0.0004 0.0006 0.0003 0.0002 0.0008
Cytoplasm 0.0068 0 0.0004 0 0.0025 0.0001 0.0016 0 0 0.0005 0.0042 0.0001 0.0003 0 0.0004 0.0002 0.0002 0.0003
Cytoplasmic Foci 0.0199 0.0002 0.006 0.0001 0.0189 0.0029 0.011 0.0001 0.001 0.007 0.0216 0.0007 0.0111 0 0.0144 0.0106 0.0035 0.0017
Eisosomes 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0003 0 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.006 0 0.0009 0 0.0024 0 0.0013 0 0 0.0047 0.0005 0.0004 0.0011 0 0.0001 0.0004 0.0005 0.0003
Endosome 0.0235 0 0.0053 0.0001 0.0259 0.0008 0.0095 0 0.0004 0.0103 0.0483 0.0032 0.0049 0 0.0008 0.0093 0.0131 0.0011
Golgi 0.0093 0 0.0058 0 0.0094 0.0003 0.0011 0 0.0004 0.0123 0.0015 0.0017 0.0016 0 0.0004 0.0161 0.0332 0.0007
Lipid Particles 0.0251 0.0007 0.0103 0.0003 0.0151 0.0106 0.0058 0 0.0019 0.0178 0.0074 0.0015 0.0243 0.0002 0.0061 0.0363 0.0073 0.0053
Mitochondria 0.0131 0.0005 0.0071 0.0002 0.0027 0.0291 0.0035 0.0003 0.0088 0.0193 0.0011 0.0328 0.0053 0.0004 0.0045 0.0075 0.0259 0.0225
None 0.0058 0 0.0007 0 0.0026 0.0001 0.002 0 0 0.0007 0.0011 0.0001 0.001 0 0.0008 0.0002 0.0007 0.0002
Nuclear Periphery 0.0128 0.0013 0.003 0.0002 0.0722 0.0009 0.0115 0.0002 0.0007 0.0079 0.0015 0.0026 0.0054 0.0064 0.0015 0.0043 0.008 0.0036
Nucleolus 0.7621 0.8869 0.8617 0.908 0.7298 0.8656 0.8256 0.8668 0.9039 0.7588 0.8347 0.8433 0.865 0.8823 0.8782 0.8131 0.8108 0.8733
Nucleus 0.052 0.1009 0.0694 0.0821 0.0679 0.0704 0.0875 0.1292 0.0797 0.1168 0.0677 0.1006 0.0621 0.108 0.0777 0.0696 0.0607 0.0752
Peroxisomes 0.0206 0 0.0062 0 0.0032 0.0032 0.001 0 0.0001 0.0107 0.0008 0.0002 0.0066 0 0.0038 0.0103 0.0031 0.0004
Punctate Nuclear 0.0093 0.0002 0.0011 0.0002 0.0304 0.0007 0.029 0.0001 0.0015 0.0008 0.0051 0.0004 0.0051 0.0002 0.0045 0.0009 0.0055 0.0019
Vacuole 0.0086 0.0042 0.0009 0.0048 0.0024 0.001 0.0036 0.001 0.0006 0.0008 0.0017 0.0008 0.0011 0.0005 0.0033 0.0007 0.0005 0.002
Vacuole Periphery 0.0033 0.0038 0.0013 0.0024 0.0062 0.0131 0.0021 0.0019 0.0003 0.0016 0.0016 0.0071 0.0021 0.0015 0.0024 0.0062 0.002 0.0085

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.3895 66.2817 67.3042 60.8285 65.1428 50.7032 60.8447 47.0177 38.6783 66.702
Translational Efficiency 2.3952 2.5071 2.3034 1.7786 2.2872 2.4851 1.9293 2.0438 2.1055 2.043

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential protein that interacts with proteasome components; has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles
Localization
Cell Percentages nucleolus (82%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Cic1

Cic1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cic1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available