Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 1 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 5 | 0 | 2 | 4 | 5 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 1 | 3 | 8 | 12 | 12 | 7 | 5 | 5 | 6 | 4 | 3 | 13 | 21 | 13 | 13 | 18 | 0 | 0 | 2 | 1 | 4 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 7 | 4 | 3 | 1 | 4 | 0 | 1 | 4 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 36 | 0 | 1 | 0 | 8 | 5 | 6 | 26 | 20 | 28 | 13 | 1 | 0 | 2 | 8 | 10 | 8 | 0 | 1 | 3 | 1 | 0 | 4 |
Nucleus | 148 | 238 | 36 | 68 | 95 | 134 | 183 | 154 | 60 | 62 | 121 | 99 | 84 | 113 | 155 | 132 | 134 | 113 | 133 | 231 | 54 | 31 | 105 | 98 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 23 | 38 | 5 | 7 | 4 | 25 | 26 | 25 | 33 | 32 | 42 | 51 | 23 | 26 | 26 | 15 | 23 | 24 | 26 | 28 | 10 | 14 | 33 | 34 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 6 |
Vac/Vac Membrane | 1 | 4 | 1 | 0 | 2 | 5 | 7 | 1 | 2 | 0 | 3 | 2 | 2 | 0 | 2 | 9 | 7 | 19 | 4 | 7 | 1 | 2 | 10 | 6 |
Unique Cell Count | 155 | 266 | 41 | 77 | 105 | 167 | 220 | 181 | 95 | 92 | 160 | 139 | 103 | 135 | 189 | 166 | 169 | 168 | 171 | 281 | 77 | 57 | 168 | 167 |
Labelled Cell Count | 174 | 320 | 43 | 82 | 109 | 190 | 237 | 198 | 130 | 124 | 205 | 175 | 116 | 155 | 214 | 189 | 195 | 192 | 171 | 281 | 77 | 57 | 168 | 167 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 7.0 | 9.5 | 9.1 | 9.6 | 7.6 | 8.0 | 7.7 | 6.3 | 6.2 | 6.3 | 7.1 | 8.7 | 8.6 | 8.1 | 8.2 | 10.0 | 10.2 | 7.6 | 10.0 | 9.4 |
Std Deviation (1e-4) | 1.8 | 1.3 | 1.8 | 1.9 | 2.4 | 1.3 | 1.7 | 1.6 | 1.5 | 1.3 | 1.4 | 2.1 | 1.9 | 2.2 | 1.9 | 2.0 | 2.4 | 2.5 | 1.7 | 2.5 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.0 | -0.33 | -0.26 | -0.32 | -0.61 | -0.62 | -0.6 | -0.42 | -0.13 | -0.16 | -0.24 | -0.22 | 0.06 | 0.1 | -0.32 | 0.07 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.2 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.1 | 0.5 | -1.1 | -0.7 | -0.4 | -2.9 | -2.5 | -1.7 | -2.2 | -0.9 | -0.6 | -0.9 | -1.2 | -1.2 | -2.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0.5 | 0 | 0.3 | 2.7 | 2.7 | 1.9 | 3.0 | 1.4 | 1.0 | 0.3 | 0 | 0.2 | 0.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.9554 | 8.1859 | 7.1417 | 6.1367 | 7.3277 | 7.1257 | 5.9674 | 7.2572 | 6.4696 | 5.429 | 5.9823 | 6.2715 | 3.3653 | 4.5245 | 3.8853 | 3.8475 | 3.7276 | 3.483 |
Actin | 0.0256 | 0 | 0.0214 | 0.0551 | 0.0002 | 0.0003 | 0.0546 | 0 | 0.0002 | 0.002 | 0.0003 | 0.0016 | 0.0199 | 0 | 0.0076 | 0 | 0.0001 | 0.0004 |
Bud | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0008 | 0.0003 | 0.0003 | 0.0002 | 0.0131 | 0.002 | 0.0023 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0013 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0014 | 0.0025 |
Bud Periphery | 0.0006 | 0.0001 | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0006 | 0.0002 |
Bud Site | 0.0018 | 0.0005 | 0.0018 | 0.0001 | 0.0008 | 0.0002 | 0.0016 | 0.0001 | 0.0012 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0 | 0.0003 | 0.0003 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0092 | 0.0001 | 0.0006 | 0.0004 | 0.0139 | 0.0004 | 0.0078 | 0.0001 | 0.1044 | 0.0164 | 0.1435 | 0.099 | 0.0027 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Cytoplasmic Foci | 0.0343 | 0 | 0.0018 | 0.0021 | 0.001 | 0.0047 | 0.0045 | 0 | 0.0049 | 0.0026 | 0.0009 | 0.0019 | 0.0033 | 0 | 0.001 | 0 | 0 | 0.0003 |
Eisosomes | 0.0004 | 0 | 0.0002 | 0.001 | 0 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0 | 0.0018 | 0.0005 | 0.0006 | 0.0001 | 0.0013 | 0 | 0.0018 | 0.0122 | 0.0045 | 0.002 | 0.0027 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 |
Endosome | 0.0191 | 0 | 0.0057 | 0.0017 | 0.001 | 0.0045 | 0.0041 | 0 | 0.0048 | 0.0075 | 0.0015 | 0.0043 | 0.0054 | 0 | 0.0024 | 0 | 0.0002 | 0.0006 |
Golgi | 0.0088 | 0 | 0.0043 | 0.0024 | 0.0001 | 0.0009 | 0.0034 | 0 | 0.0002 | 0.0007 | 0.0001 | 0.0006 | 0.0019 | 0 | 0.0045 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0231 | 0 | 0.0075 | 0.0028 | 0.0003 | 0.0017 | 0.0032 | 0 | 0.0021 | 0.0019 | 0.0001 | 0.0004 | 0.0049 | 0 | 0.0006 | 0 | 0.0001 | 0.0005 |
Mitochondria | 0.0169 | 0.0002 | 0.0271 | 0.0093 | 0.0003 | 0.0002 | 0.0017 | 0.0001 | 0.0008 | 0.0006 | 0.0003 | 0.0002 | 0.004 | 0.0001 | 0.0044 | 0.0001 | 0.0007 | 0.0003 |
None | 0.0176 | 0 | 0.0004 | 0.0057 | 0.1075 | 0.0001 | 0.074 | 0 | 0.0029 | 0.0045 | 0.0068 | 0.0005 | 0.001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0232 | 0.0007 | 0.0134 | 0.0013 | 0.0022 | 0.0004 | 0.0113 | 0.0008 | 0.0034 | 0.0047 | 0.0059 | 0.0035 | 0.0372 | 0.0006 | 0.001 | 0.0005 | 0.0045 | 0.002 |
Nucleolus | 0.0376 | 0.0344 | 0.0227 | 0.0094 | 0.1164 | 0.0744 | 0.0383 | 0.0298 | 0.0341 | 0.0158 | 0.0279 | 0.0555 | 0.0221 | 0.0192 | 0.0164 | 0.0086 | 0.1493 | 0.0667 |
Nucleus | 0.702 | 0.9626 | 0.8783 | 0.8988 | 0.7313 | 0.905 | 0.7327 | 0.9679 | 0.8285 | 0.9259 | 0.8013 | 0.8249 | 0.8774 | 0.9761 | 0.9583 | 0.99 | 0.838 | 0.9188 |
Peroxisomes | 0.0407 | 0 | 0.0015 | 0.0077 | 0.0001 | 0.0013 | 0.0025 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0051 | 0 | 0.0007 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0333 | 0.001 | 0.0034 | 0.0009 | 0.0097 | 0.0033 | 0.0512 | 0.0009 | 0.0078 | 0.0024 | 0.0042 | 0.0036 | 0.0073 | 0.0018 | 0.0005 | 0.0004 | 0.0025 | 0.0058 |
Vacuole | 0.001 | 0.0001 | 0.0029 | 0.0001 | 0.0007 | 0.0001 | 0.0025 | 0 | 0.0017 | 0.0012 | 0.0015 | 0.0002 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0009 | 0.0003 |
Vacuole Periphery | 0.0011 | 0 | 0.0028 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0024 | 0 | 0.0003 | 0 | 0.0008 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.396 | 22.2157 | 9.0667 | 14.8473 | 17.7215 | 12.4188 | 14.8812 | 22.7408 | 16.223 | 14.7977 |
Translational Efficiency | 1.3172 | 0.9309 | 2.4514 | 1.5846 | 1.3894 | 1.9066 | 1.6713 | 1.0627 | 1.4994 | 1.9212 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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