Standard name
Human Ortholog
Description Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0.06 0 0.05 0.06 0 0
Bud 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.13 0.07 0.07 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.12 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.68 0.78 0.84 0.84 0.94 0.89 0.8 0.88 0.68 0.81 0.76 0.82 0.81 0.95 0.97 0.69 0.66 0.73 0.57 0.44 0.54 0.59 0.75 0.79
Endoplasmic Reticulum 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0 0 0.14 0 0.15 0.28 0.15 0.41 0.28 0.42 0.25 0 0 0 0 0 0 0.08 0 0.05 0 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.2 0.24 0.19 0.09 0.15 0.1 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 6 5 2 0 0 0 0 0 0 0 0 17 1 0 17 1 8 6 7 6 17 16 6
Bud 5 7 4 5 1 2 5 5 2 10 3 9 8 1 0 2 2 2 4 21 9 20 11 13
Bud Neck 0 0 2 1 6 4 1 6 1 6 1 7 2 0 0 0 0 0 2 12 6 33 13 4
Bud Site 0 2 0 0 0 0 0 2 2 1 0 2 6 0 0 6 3 4 – – – – – –
Cell Periphery 0 1 0 1 0 0 0 2 2 1 1 4 4 8 5 8 2 2 0 0 0 3 6 5
Cytoplasm 42 107 79 102 238 214 238 304 162 271 201 252 271 392 476 170 118 135 56 77 74 170 350 364
Endoplasmic Reticulum 3 12 2 0 0 0 0 0 2 0 0 1 6 5 0 8 3 6 1 0 1 2 1 2
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 4 13 6 1 3 2 8 5 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 2 0 0 0 1 2 2
Mitochondria 22 3 1 17 4 35 83 51 98 93 112 76 0 1 0 3 3 1 8 6 6 8 31 20
Nucleus 0 2 1 2 1 0 4 6 4 0 4 2 13 5 1 5 8 1 3 13 5 2 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 3 1 2 0 0 0 0 0 0
SpindlePole 0 0 3 2 4 2 1 7 0 4 3 6 14 1 3 11 9 6 2 3 4 7 11 15
Vac/Vac Membrane 1 11 4 5 7 0 1 0 1 1 2 2 20 7 18 48 42 35 9 26 13 5 4 5
Unique Cell Count 62 138 94 121 253 241 299 347 240 336 265 306 334 411 493 245 178 185 99 175 137 287 467 461
Labelled Cell Count 74 151 102 137 261 257 333 384 275 387 327 361 365 423 506 286 205 211 99 175 137 287 467 461


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.9 5.0 4.4 5.0 3.7 3.5 4.0 3.1 3.3 3.1 3.3 5.5 5.6 5.6 7.1 7.3 7.4 4.9 5.0 5.2
Std Deviation (1e-4) 0.4 0.9 1.2 1.2 1.9 1.0 1.5 1.6 0.9 1.0 1.2 1.1 1.1 1.2 1.8 1.3 1.7 1.7 1.1 1.6 1.8
Intensity Change (Log2) – – – -0.16 0.01 -0.41 -0.49 -0.3 -0.69 -0.59 -0.69 -0.59 0.14 0.18 0.17 0.52 0.56 0.57 -0.01 0.0 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.5 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 3.0 1.2 -1.0 0.9 -3.0 -0.7 -1.6 -0.4 -0.6 4.0 4.9 -2.7 -3.1 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0.6 0 0 3.5 4.0 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2569 -0.3166 -0.0469 0.0342 -0.2796 -0.2317 -0.1666 0.2937 0.8978 1.1451 0.9731 0.5523 -0.4108 0.3548 0.4559 0.9008 0.9163 0.3019
Actin 0.0549 0.0042 0.0168 0.0009 0.0048 0.003 0.0432 0.0033 0.0158 0.0029 0.0112 0.0054 0.0336 0.0034 0.0136 0.008 0.0832 0.009
Bud 0.0045 0.002 0.0291 0.0012 0.0077 0.0057 0.0095 0.0068 0.0381 0.0011 0.0038 0.0008 0.0229 0.0045 0.0436 0.0059 0.0055 0.0018
Bud Neck 0.0243 0.0025 0.0076 0.0009 0.0014 0.0522 0.0251 0.0053 0.0023 0.0017 0.0166 0.1065 0.018 0.0042 0.0041 0.0273 0.0045 0.0993
Bud Periphery 0.0176 0.0031 0.0396 0.001 0.0171 0.0048 0.0221 0.0054 0.0538 0.001 0.0071 0.0015 0.0384 0.0101 0.071 0.0078 0.0135 0.0021
Bud Site 0.0316 0.0405 0.0197 0.0018 0.002 0.0061 0.0505 0.0555 0.0234 0.0014 0.0065 0.0006 0.0232 0.0339 0.0205 0.0055 0.0162 0.0013
Cell Periphery 0.001 0.0027 0.0005 0.0001 0.0002 0.0002 0.001 0.0009 0.001 0.0001 0.0004 0.0001 0.0009 0.0012 0.0006 0.0004 0.0005 0.0002
Cytoplasm 0.1057 0.2937 0.2734 0.371 0.4377 0.2643 0.2422 0.3444 0.3851 0.5728 0.4051 0.3819 0.3049 0.4889 0.3826 0.429 0.4827 0.503
Cytoplasmic Foci 0.0911 0.0326 0.0265 0.0106 0.0155 0.0299 0.0713 0.0261 0.0228 0.0105 0.0511 0.0467 0.0779 0.007 0.0419 0.0181 0.0414 0.0594
Eisosomes 0.0009 0.0005 0.0003 0.0001 0.0002 0.0001 0.0008 0.0003 0.0003 0 0.0003 0.0001 0.0006 0.0002 0.0001 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0121 0.0026 0.0072 0.005 0.0095 0.0021 0.0046 0.0034 0.0057 0.004 0.0058 0.0018 0.0082 0.0048 0.0077 0.015 0.0089 0.0037
Endosome 0.0566 0.0029 0.0167 0.0048 0.0065 0.005 0.0223 0.0066 0.0119 0.003 0.0315 0.0046 0.0196 0.0027 0.0144 0.0411 0.0302 0.0111
Golgi 0.0154 0.0007 0.0055 0.0003 0.0022 0.0013 0.0052 0.0009 0.0023 0.0003 0.0102 0.0008 0.0043 0.0003 0.0042 0.0024 0.0087 0.0022
Lipid Particles 0.0279 0.0005 0.0047 0.0002 0.0131 0.0091 0.0076 0.0005 0.0022 0.0003 0.0432 0.0026 0.0092 0.0002 0.0052 0.0016 0.0051 0.0026
Mitochondria 0.0222 0.0015 0.0125 0.0014 0.002 0.0086 0.0051 0.0016 0.0103 0.0009 0.0141 0.0023 0.0128 0.0006 0.0072 0.0068 0.0034 0.0072
None 0.4305 0.5536 0.4654 0.4112 0.3662 0.5563 0.3926 0.4589 0.2657 0.1713 0.2581 0.356 0.345 0.2749 0.1408 0.1326 0.1236 0.2004
Nuclear Periphery 0.0347 0.0032 0.0068 0.0114 0.0082 0.0074 0.0175 0.0028 0.0087 0.0042 0.0198 0.0032 0.0095 0.0046 0.0118 0.026 0.0265 0.0064
Nucleolus 0.0016 0.0009 0.0013 0.0015 0.001 0.0017 0.0023 0.0014 0.0022 0.001 0.0109 0.0012 0.0028 0.0008 0.0025 0.0017 0.0016 0.0008
Nucleus 0.0112 0.0385 0.0364 0.0873 0.0782 0.0242 0.0326 0.0585 0.1133 0.2085 0.0461 0.051 0.0307 0.1487 0.1748 0.2441 0.0935 0.0687
Peroxisomes 0.0321 0.0043 0.01 0.0007 0.0091 0.0026 0.0153 0.0027 0.0102 0.0011 0.0194 0.0039 0.0192 0.0003 0.0186 0.0012 0.0201 0.0054
Punctate Nuclear 0.0156 0.008 0.0153 0.0861 0.015 0.0119 0.0242 0.0119 0.0203 0.0124 0.0335 0.028 0.0106 0.0065 0.0287 0.0129 0.0261 0.0128
Vacuole 0.0045 0.0015 0.0036 0.0021 0.002 0.0021 0.0038 0.0026 0.0038 0.0012 0.0038 0.0009 0.0061 0.002 0.0053 0.0092 0.0034 0.0017
Vacuole Periphery 0.0041 0.0002 0.0011 0.0005 0.0004 0.0012 0.0009 0.0003 0.0008 0.0002 0.0013 0.0002 0.0016 0.0002 0.0011 0.003 0.0015 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.0115 15.6568 3.8727 7.6053 16.6526 23.3395 12.6644 9.2599 9.6672 18.2506
Translational Efficiency 1.6999 1.3228 1.7327 1.1908 1.29 1.0218 1.0764 1.1452 0.9618 1.1644

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gic1

Gic1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gic1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available