Standard name | |
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Human Ortholog | |
Description | Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 2 | – | 4 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 3 | 2 | 3 |
Bud | 0 | 0 | 3 | 0 | 1 | 2 | 0 | 1 | 1 | – | 3 | 3 | 4 | 0 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 3 | 3 | 0 | 3 | 12 | 20 | 23 | 24 | – | 15 | 9 | 9 | 12 | 42 | 22 | 12 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 363 | 337 | 346 | 263 | 279 | 375 | 376 | 472 | 311 | – | 168 | 219 | 761 | 913 | 946 | 497 | 197 | 258 | 349 | 322 | 345 | 501 | 508 | 595 |
Endoplasmic Reticulum | 7 | 6 | 6 | 5 | 6 | 17 | 25 | 34 | 11 | – | 5 | 7 | 3 | 10 | 7 | 13 | 10 | 5 | 4 | 0 | 5 | 15 | 17 | 22 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 4 | 1 | 3 | 6 | 10 | 14 |
Golgi | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 2 | 5 | 1 | 0 | 3 | 4 | 2 |
Mitochondria | 1 | 0 | 2 | 3 | 5 | 16 | 24 | 64 | 68 | – | 60 | 61 | 3 | 0 | 6 | 3 | 1 | 4 | 6 | 0 | 4 | 3 | 4 | 2 |
Nucleus | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 0 | 0 | 2 | 4 | 5 | 7 | 10 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | – | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 1 | 1 | 0 | 0 | 0 | 1 | 37 | 5 | – | 4 | 13 | 4 | 2 | 2 | 29 | 9 | 2 | 2 | 4 | 9 | 25 | 21 | 36 |
Unique Cell Count | 375 | 346 | 356 | 267 | 285 | 397 | 418 | 585 | 376 | 219 | 282 | 770 | 927 | 962 | 526 | 212 | 271 | 383 | 335 | 374 | 571 | 579 | 694 | |
Labelled Cell Count | 387 | 351 | 362 | 271 | 295 | 424 | 448 | 640 | 422 | 261 | 315 | 785 | 943 | 1027 | 572 | 239 | 295 | 383 | 335 | 374 | 571 | 579 | 694 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 7.9 | 5.9 | 6.2 | 9.0 | 9.4 | 9.9 | 10.0 | 8.8 | – | 9.0 | 8.7 | 8.8 | 9.2 | 8.3 | 11.1 | 11.6 | 11.5 | 7.8 | 8.3 | 9.0 |
Std Deviation (1e-4) | 1.0 | 1.0 | 0.7 | 0.9 | 1.2 | 1.5 | 1.8 | 2.2 | 2.2 | – | 2.7 | 2.8 | 1.2 | 1.3 | 1.2 | 1.8 | 2.2 | 2.3 | 1.2 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.61 | 0.67 | 0.73 | 0.76 | 0.57 | – | 0.6 | 0.55 | 0.57 | 0.63 | 0.49 | 0.91 | 0.96 | 0.95 | 0.4 | 0.49 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.1 | 3.2 | 2.8 | 4.0 | – | 4.0 | 2.2 | 0 | 0 | 3.1 | 2.9 | 3.5 | 3.1 |
Cytoplasm | 1.1 | 0.6 | -1.9 | -4.0 | -7.3 | -6.5 | – | -7.7 | -7.7 | 2.0 | 1.5 | 1.3 | -1.9 | -2.4 | -1.3 |
Endoplasmic Reticulum | 0 | 0.4 | 2.1 | 3.0 | 3.0 | 1.1 | – | 0 | 0.7 | 0 | 0 | 0 | 0.8 | 2.1 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.1 | 4.0 | 6.0 | 8.1 | – | 10.1 | 8.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 4.6 | 0 | – | 0 | 3.7 | 0 | 0 | 0 | 4.2 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7093 | 6.655 | 6.1558 | 6.2816 | 6.5368 | 6.0436 | 3.4831 | 4.7199 | 4.6285 | 4.3034 | 4.4898 | 4.6821 | 9.2831 | 9.5459 | 10.0246 | 9.613 | 9.9471 | 9.5446 |
Actin | 0.0268 | 0.0004 | 0.0197 | 0.0932 | 0.0575 | 0.0001 | 0.0093 | 0.0006 | 0.001 | 0.0001 | 0 | 0.0001 | 0.0014 | 0.002 | 0.0014 | 0 | 0.0001 | 0.0005 |
Bud | 0.001 | 0.0011 | 0.0007 | 0.0027 | 0.0069 | 0.0004 | 0.0004 | 0.0021 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0028 | 0.0094 | 0.024 | 0.0002 | 0.0026 | 0.0039 |
Bud Neck | 0.003 | 0.0002 | 0.0015 | 0.0014 | 0.0046 | 0.0007 | 0.0004 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0007 | 0.0004 | 0.0004 | 0.0043 | 0.0003 | 0.0002 | 0.0007 |
Bud Periphery | 0.0012 | 0.0002 | 0.0005 | 0.0035 | 0.0078 | 0.0008 | 0.0003 | 0.0013 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0008 | 0.0021 | 0.0074 | 0 | 0.0006 | 0.0009 |
Bud Site | 0.0066 | 0.0082 | 0.0027 | 0.0058 | 0.0065 | 0.0001 | 0.0006 | 0.0059 | 0.0008 | 0.0004 | 0 | 0.0001 | 0.0029 | 0.0076 | 0.0156 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.001 | 0.0004 | 0.0006 | 0.0004 | 0.0013 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 |
Cytoplasm | 0.5956 | 0.9623 | 0.8625 | 0.7447 | 0.5844 | 0.9226 | 0.8453 | 0.9194 | 0.8954 | 0.8651 | 0.9271 | 0.9103 | 0.8863 | 0.8487 | 0.8307 | 0.8429 | 0.9045 | 0.9313 |
Cytoplasmic Foci | 0.0273 | 0.0046 | 0.0255 | 0.0178 | 0.0345 | 0.0072 | 0.0152 | 0.0095 | 0.0156 | 0.0165 | 0.0127 | 0.014 | 0.0161 | 0.0162 | 0.0206 | 0.02 | 0.0249 | 0.0138 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0591 | 0.0026 | 0.0102 | 0.004 | 0.0213 | 0.0178 | 0.0325 | 0.0085 | 0.0086 | 0.0029 | 0.003 | 0.0148 | 0.0058 | 0.0026 | 0.0036 | 0.0037 | 0.0015 | 0.0029 |
Endosome | 0.0903 | 0.0041 | 0.033 | 0.0459 | 0.1362 | 0.0279 | 0.0399 | 0.0175 | 0.0364 | 0.063 | 0.0363 | 0.032 | 0.0284 | 0.0324 | 0.0405 | 0.0519 | 0.0431 | 0.0308 |
Golgi | 0.006 | 0.0003 | 0.0077 | 0.0219 | 0.0595 | 0.0005 | 0.0018 | 0.001 | 0.0012 | 0.0003 | 0.0003 | 0.0006 | 0.0013 | 0.0013 | 0.0036 | 0.0003 | 0.0005 | 0.0008 |
Lipid Particles | 0.0055 | 0.0001 | 0.0038 | 0.0038 | 0.0077 | 0.0001 | 0.0007 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0 | 0 | 0 |
Mitochondria | 0.0044 | 0.0001 | 0.002 | 0.0166 | 0.0173 | 0.0012 | 0.0016 | 0.001 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0011 | 0.0021 | 0.0023 | 0.0002 | 0.0002 | 0.0006 |
None | 0.0265 | 0.008 | 0.007 | 0.009 | 0.0026 | 0.0023 | 0.0166 | 0.0114 | 0.0179 | 0.0021 | 0.0017 | 0.0094 | 0.03 | 0.056 | 0.018 | 0.042 | 0.0056 | 0.0043 |
Nuclear Periphery | 0.0476 | 0.0006 | 0.0018 | 0.0015 | 0.0182 | 0.0034 | 0.0051 | 0.0024 | 0.0012 | 0.0014 | 0.0009 | 0.0017 | 0.0013 | 0.0015 | 0.0016 | 0.0009 | 0.0005 | 0.0006 |
Nucleolus | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0008 | 0 | 0 | 0 |
Nucleus | 0.0348 | 0.001 | 0.0014 | 0.001 | 0.0036 | 0.0019 | 0.0029 | 0.0048 | 0.0017 | 0.0025 | 0.0012 | 0.002 | 0.0017 | 0.0034 | 0.0044 | 0.0019 | 0.001 | 0.0019 |
Peroxisomes | 0.0025 | 0 | 0.0091 | 0.0028 | 0.0028 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0.0011 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0214 | 0.0002 | 0.0009 | 0.0005 | 0.0029 | 0.0001 | 0.0013 | 0.0007 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0004 | 0.0014 | 0.0015 | 0.0002 | 0.0001 | 0.0002 |
Vacuole | 0.0317 | 0.0047 | 0.0079 | 0.0184 | 0.0187 | 0.0101 | 0.0222 | 0.0106 | 0.0158 | 0.0353 | 0.0137 | 0.0111 | 0.0163 | 0.0101 | 0.0153 | 0.0295 | 0.0112 | 0.005 |
Vacuole Periphery | 0.0056 | 0.0005 | 0.0015 | 0.0044 | 0.0053 | 0.0029 | 0.0031 | 0.0014 | 0.0022 | 0.0085 | 0.0024 | 0.0019 | 0.002 | 0.0022 | 0.0026 | 0.0056 | 0.0031 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.1342 | 46.6055 | 32.9252 | 55.8535 | 37.27 | 38.5442 | 52.529 | 57.0742 | 49.0662 | 60.6749 |
Translational Efficiency | 0.5944 | 0.7151 | 0.7614 | 0.4491 | 0.6647 | 0.9968 | 0.6638 | 0.5061 | 0.6119 | 0.5336 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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