Standard name
Human Ortholog
Description Component of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0.05 0.07
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.05 0.08 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.1 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0.06 0.13 0.07 0 0.06 0 0 0 0 0.1 0.12 0.12 0.07 0 0 0.09 0.06 0.1 0.1 0.09 0.09
Golgi 0 0.06 0.17 0.08 0.07 0 0 0 0 0 0 0 0.15 0.2 0.33 0.28 0.5 0.27 0.06 0.07 0.08 0.07 0.1 0.11
Mitochondria 0.97 0.89 0.7 0.87 0.69 0.87 0.89 0.86 0.91 0.93 0.95 0.92 0.77 0.66 0.48 0.1 0.08 0.08 0.6 0.75 0.61 0.52 0.49 0.48
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0.08 0.06 0 0 0 0 0 0 0 0.17 0.24 0.15 0.18 0.13 0.29 0.19 0 0.14 0.23 0.24 0.21
SpindlePole 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0 0.12 0.1 0.06 0.05 0.05 0.07 0.06 0.08 0.06 0.06 0.11 0.18 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 12 6 8 0 0 3 0 1 1 0 18 16 8 7 9 10 4 4 3 15 34 51
Bud 6 0 5 8 12 9 13 10 13 16 18 3 22 8 16 10 7 11 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 1 1
Bud Site 2 0 0 0 0 2 7 6 5 4 6 2 2 0 0 2 0 1 – – – – – –
Cell Periphery 4 8 8 12 6 3 6 2 3 3 2 2 20 26 21 14 5 20 0 0 0 0 1 3
Cytoplasm 1 3 5 8 16 13 15 18 11 4 11 4 10 14 22 12 7 10 0 0 0 0 0 0
Endoplasmic Reticulum 11 15 4 1 6 1 2 0 2 5 3 8 7 10 5 6 2 8 0 1 1 0 1 5
Endosome 1 1 6 12 47 21 14 27 3 7 0 7 59 87 86 14 4 8 23 10 14 41 67 73
Golgi 1 10 20 17 26 1 2 2 0 1 0 5 90 143 236 55 46 53 15 11 12 28 75 83
Mitochondria 252 141 80 184 248 272 398 420 303 417 307 365 463 459 344 20 7 16 157 130 88 225 372 374
Nucleus 0 0 0 0 5 4 7 5 2 6 2 8 3 2 3 6 1 3 0 0 0 0 1 0
Nuclear Periphery 10 2 3 0 4 9 12 15 19 19 14 16 6 10 9 5 3 9 4 0 0 4 3 6
Nucleolus 5 1 0 0 2 5 16 15 12 9 15 10 4 4 1 5 2 9 0 0 0 0 3 1
Peroxisomes 7 2 3 17 23 12 11 13 8 2 0 1 104 168 110 35 12 56 51 6 20 99 186 164
SpindlePole 5 0 5 9 20 10 18 26 14 13 7 10 30 37 39 11 4 7 1 0 0 2 3 4
Vac/Vac Membrane 11 17 5 8 45 30 28 26 17 33 19 32 34 40 80 35 8 17 1 6 1 6 5 4
Unique Cell Count 260 159 115 211 360 312 446 489 334 448 324 396 599 699 716 196 92 194 264 174 145 432 766 781
Labelled Cell Count 319 204 156 282 468 392 549 588 413 540 405 473 872 1024 981 237 117 238 264 174 145 432 766 781


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.4 6.6 6.2 6.4 5.6 5.2 5.8 5.0 5.2 4.6 5.2 7.3 7.2 7.5 8.0 9.5 9.2 6.5 6.7 7.0
Std Deviation (1e-4) 0.6 1.0 1.5 1.4 1.4 1.3 1.3 1.6 1.3 0.9 1.1 1.0 1.1 1.0 1.3 1.5 2.2 1.8 1.2 1.3 1.4
Intensity Change (Log2) – – – -0.11 -0.04 -0.25 -0.36 -0.2 -0.42 -0.36 -0.53 -0.34 0.13 0.12 0.18 0.28 0.52 0.47 -0.02 0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.9 0 0 0 0 0 0 0 0 0 0 0 -2.4 -0.2 -1.7
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.3 1.0 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 0 0 0 0 0 0 0 0 0 0 0 0.1 -0.4 1.0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.7 1.0 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.2 2.3 0.6 0 0.1 0 0 0 0 1.6 2.3 2.2 0.7 0 -0.4
Golgi -2.5 -3.2 -7.2 0 0 -7.8 0 0 -7.1 -0.6 0.8 3.4 2.1 5.0 2.0
Mitochondria 3.9 -0.1 4.2 5.3 4.2 5.5 7.0 0 6.4 1.8 -0.8 -4.3 -10.8 -9.0 -11.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1.3 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2.0 1.6 0 0 0 0 0 0 0 4.1 5.2 3.7 4.0 2.9 5.7
SpindlePole 0 0.5 0 0 0.4 0 0 0 0 0.3 0.4 0.5 0.5 0 0
Vacuole 0 2.5 1.8 0.8 0.4 0.3 1.2 0.6 1.4 0.6 0.6 2.2 3.4 1.3 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.289 1.6901 1.8252 1.1034 1.5928 1.8885 2.1406 2.5978 2.1624 1.7767 1.7555 2.3147 1.5016 1.2766 1.1993 1.5714 1.0324 1.3912
Actin 0.0449 0.0436 0.0394 0.0249 0.0881 0.0182 0.0657 0.0164 0.0468 0.0794 0.0259 0.028 0.0906 0.0146 0.0268 0.015 0.065 0.029
Bud 0.0005 0.0012 0.0006 0.0013 0.0018 0.0003 0.0007 0.0013 0.0013 0.0009 0.0007 0.0003 0.0011 0.0034 0.0011 0.0018 0.0004 0.0003
Bud Neck 0.0004 0.0007 0.0084 0.0013 0.0003 0.0008 0.0039 0.0009 0.0032 0.0007 0.0009 0.0008 0.0018 0.0013 0.0013 0.0036 0.0002 0.0044
Bud Periphery 0.0009 0.0016 0.0008 0.0018 0.006 0.0006 0.0011 0.0019 0.0021 0.0015 0.0023 0.0006 0.0016 0.0066 0.002 0.0027 0.0009 0.0006
Bud Site 0.001 0.0046 0.0063 0.0127 0.0008 0.0005 0.004 0.019 0.0155 0.002 0.0013 0.0004 0.0094 0.0438 0.0046 0.0106 0.0004 0.0007
Cell Periphery 0.0004 0.0003 0.0003 0.0004 0.0003 0.0003 0.0004 0.0005 0.0007 0.0002 0.0002 0.0002 0.0004 0.0028 0.0009 0.0005 0.0001 0.0003
Cytoplasm 0.0013 0.0037 0.0089 0.0009 0.0002 0.001 0.01 0.0052 0.0032 0.0007 0.0007 0.0006 0.004 0.0237 0.0035 0.0053 0.0011 0.0008
Cytoplasmic Foci 0.0245 0.0415 0.0271 0.03 0.0027 0.0137 0.0515 0.0452 0.0348 0.039 0.0642 0.0181 0.0331 0.0429 0.0209 0.0241 0.0125 0.0133
Eisosomes 0.0008 0.0004 0.0004 0.0005 0.0008 0.0005 0.0012 0.0004 0.0012 0.0007 0.0003 0.0003 0.0011 0.0006 0.0025 0.0004 0.0003 0.0005
Endoplasmic Reticulum 0.0022 0.0007 0.003 0.0006 0.0019 0.0007 0.0021 0.0011 0.0005 0.0004 0.0004 0.001 0.0031 0.0029 0.0028 0.0007 0.0005 0.0004
Endosome 0.0772 0.077 0.0858 0.0788 0.0246 0.0399 0.0649 0.0641 0.0641 0.05 0.0512 0.0565 0.0908 0.1523 0.0646 0.0705 0.0618 0.0391
Golgi 0.0671 0.1596 0.0658 0.1175 0.031 0.1107 0.0877 0.0984 0.0788 0.1064 0.0351 0.1013 0.1196 0.1087 0.0923 0.1236 0.1885 0.1016
Lipid Particles 0.0662 0.0405 0.0085 0.0164 0.0045 0.0262 0.0779 0.0189 0.02 0.0136 0.0358 0.0265 0.0385 0.0165 0.0195 0.0038 0.0015 0.0107
Mitochondria 0.5968 0.5501 0.6701 0.6078 0.8193 0.7197 0.4664 0.6447 0.6163 0.6194 0.6164 0.6751 0.5017 0.4859 0.6969 0.6958 0.6305 0.7436
None 0.0049 0.0049 0.0009 0.0004 0.0007 0.0042 0.0306 0.0009 0.0031 0.0004 0.0004 0.0003 0.0051 0.0123 0.0069 0.0004 0.0001 0.0003
Nuclear Periphery 0.0087 0.0006 0.0142 0.0006 0.0037 0.0015 0.0072 0.0002 0.0006 0.0001 0.0003 0.0062 0.0041 0.0013 0.0009 0.0003 0.0001 0.0002
Nucleolus 0.0007 0.0003 0.0003 0.0004 0.0002 0.0002 0.0018 0.0006 0.0012 0.0001 0.0005 0.0002 0.001 0.0042 0.0007 0.0001 0 0.0001
Nucleus 0.0007 0.0004 0.0069 0.0004 0.0003 0.0003 0.0086 0.0004 0.0018 0.0001 0.0003 0.0004 0.0122 0.003 0.0004 0.0002 0.0001 0.0001
Peroxisomes 0.0825 0.0614 0.0456 0.0988 0.0049 0.0558 0.0975 0.0719 0.0967 0.0814 0.1445 0.0724 0.0685 0.0424 0.0425 0.0224 0.0321 0.0444
Punctate Nuclear 0.0003 0.0003 0.0013 0.0003 0.0003 0.0009 0.0083 0.0003 0.0022 0.0006 0.0077 0.005 0.0052 0.0022 0.0002 0.0001 0 0.0001
Vacuole 0.0093 0.0036 0.0026 0.0022 0.0034 0.0016 0.0054 0.0049 0.0034 0.0009 0.0062 0.0022 0.0036 0.0197 0.0049 0.0078 0.0013 0.0038
Vacuole Periphery 0.0085 0.0032 0.0028 0.0023 0.004 0.0024 0.003 0.0026 0.0027 0.0016 0.0046 0.0035 0.0037 0.0087 0.0039 0.0103 0.0024 0.0056

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.3529 25.9048 38.445 42.6064 44.0744 30.0762 41.0515 45.555 48.6234 45.3894
Translational Efficiency 0.6162 0.8417 0.6185 0.4935 0.5282 0.7753 0.5834 0.4954 0.5651 0.5228

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
Localization
Cell Percentages mitochondrion (84%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Sam35

Sam35


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sam35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available