Standard name
Human Ortholog
Description Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.98 0.93 0.91 – 0.62 0.73 0.73 0.69 0.64 0.98 0.94 0.97 0.95 0.88 0.94 0.97 0.91 0.92 0.87 0.83 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0.05 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0.05 0.08 0.05 0.13 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.08 – 0.28 0.16 0.16 0.14 0.16 0 0.05 0 0 0.17 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 – 1 1 4 3 4 0 1 0 0 0 0 0 1 0 0 1 0
Bud 0 0 11 3 0 0 – 8 1 7 7 4 0 0 0 0 0 0 0 9 10 1 8 19
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 4 0 0 1 3 – 14 6 7 5 14 0 1 2 2 0 1 0 0 0 0 0 0
Cytoplasm 144 298 304 316 325 340 – 346 174 206 196 209 157 298 370 105 66 131 137 272 291 112 203 155
Endoplasmic Reticulum 0 0 0 1 2 2 – 20 5 13 17 27 1 0 3 3 0 2 0 0 1 1 1 1
Endosome 0 0 1 0 0 0 – 1 0 0 0 0 0 1 2 0 0 1 0 1 0 1 8 3
Golgi 0 0 0 0 0 0 – 2 1 0 0 3 0 0 1 1 0 0 0 0 0 0 6 3
Mitochondria 0 1 9 10 2 13 – 27 19 15 38 28 0 2 0 4 3 1 0 1 1 0 1 3
Nucleus 0 0 2 1 1 0 – 6 3 5 1 5 0 0 0 1 0 0 0 2 0 3 2 2
Nuclear Periphery 0 0 0 0 0 1 – 3 0 1 0 3 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 – 3 1 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 2 2 0 0 0 0 3 2 0 0 4
Vac/Vac Membrane 1 13 6 0 27 29 – 157 37 46 39 53 5 15 12 3 13 14 0 1 1 3 5 2
Unique Cell Count 145 305 316 321 349 374 555 237 282 286 325 161 318 383 110 75 140 142 299 318 129 246 198
Labelled Cell Count 145 316 334 331 358 388 588 248 304 306 351 163 322 392 119 82 150 142 299 318 129 246 198


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 9.8 7.4 10.3 14.9 18.2 – 22.9 22.9 28.6 32.3 32.1 13.4 24.5 25.5 13.7 13.4 21.1 17.5 12.2 13.5
Std Deviation (1e-4) 2.6 6.7 3.6 8.8 8.9 14.2 – 16.0 16.2 19.5 39.9 26.3 25.7 39.8 21.1 9.4 13.8 36.6 68.1 25.9 31.2
Intensity Change (Log2) – – – 0.48 1.01 1.29 – 1.63 1.63 1.95 2.12 2.12 0.86 1.72 1.78 0.88 0.86 1.51 1.24 0.72 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud -2.2 -3.5 -3.6 – -2.0 -2.4 -0.7 -0.7 -1.9 0 -3.4 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 2.8 0 0 0 3.7 0 0 0 0 0 0
Cytoplasm 1.8 -1.8 -2.8 – -11.0 -7.7 -8.0 -9.0 -10.1 0.8 -1.4 0.3 -0.3 -2.8 -1.2
Endoplasmic Reticulum 0 0 0 – 3.4 0 3.9 4.4 5.2 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 -2.3 0.5 – 1.4 2.7 1.5 4.8 3.1 0 -2.1 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.5 3.5 – 9.6 6.0 6.2 5.5 6.3 0 2.0 1.0 0 0 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.1167 26.1853 27.0943 23.6301 25.8071 25.1273 17.9713 21.2176 18.2515 16.186 16.1114 17.9706 13.507 18.6317 14.2416 15.6307 10.7144 16.4603
Actin 0.0395 0.002 0.0232 0.0002 0.0449 0.0012 0.0095 0.006 0.0175 0.0275 0.0251 0.0135 0.0247 0.0007 0.007 0.0122 0.0147 0.0012
Bud 0.0013 0.0033 0.0041 0.0002 0.0025 0.0016 0.0013 0.0086 0.0019 0.0021 0.0073 0.0022 0.001 0.0017 0.0019 0.0003 0.0008 0.0003
Bud Neck 0.006 0.0003 0.0006 0.0004 0.0006 0.0006 0.0038 0.0004 0.0011 0.0017 0.0009 0.0016 0.0008 0.0002 0.0005 0.0005 0.0006 0.001
Bud Periphery 0.0006 0.0008 0.0011 0 0.0024 0.0003 0.0008 0.0023 0.0011 0.0012 0.0069 0.0019 0.0008 0.0005 0.0007 0.0004 0.0004 0.0001
Bud Site 0.0054 0.0014 0.0017 0 0.0037 0.0001 0.0057 0.008 0.0053 0.0012 0.0013 0.0005 0.0027 0.0012 0.002 0.0014 0.0025 0
Cell Periphery 0.0002 0.0001 0.0001 0 0.0005 0 0.0002 0.0001 0.0003 0.0006 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.8482 0.9671 0.8945 0.9647 0.7589 0.9477 0.852 0.9449 0.8621 0.7708 0.8821 0.906 0.8578 0.9837 0.9266 0.7805 0.9123 0.9689
Cytoplasmic Foci 0.0162 0.0055 0.0112 0.0018 0.0449 0.0042 0.0167 0.0021 0.0193 0.0141 0.0056 0.0087 0.0119 0.0014 0.01 0.0108 0.0131 0.0033
Eisosomes 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0.0002 0 0.0001 0.0002 0 0 0.0002 0.0002 0
Endoplasmic Reticulum 0.0032 0.0006 0.0013 0.0005 0.0014 0.0006 0.0073 0.001 0.0037 0.0245 0.0029 0.0027 0.0054 0.0005 0.0022 0.0035 0.0063 0.0009
Endosome 0.0067 0.0022 0.0128 0.0006 0.0097 0.0004 0.0194 0.0003 0.0233 0.0169 0.0243 0.0072 0.0294 0.0002 0.0065 0.0359 0.006 0.0005
Golgi 0.003 0.0002 0.0034 0 0.0102 0 0.0073 0.0001 0.0073 0.0116 0.002 0.0021 0.0065 0 0.0048 0.0203 0.002 0
Lipid Particles 0.0101 0.0002 0.0048 0 0.011 0 0.0069 0 0.0047 0.0407 0.0037 0.0025 0.0055 0 0.0123 0.0054 0.0033 0
Mitochondria 0.0013 0.0004 0.002 0.0001 0.0299 0.0004 0.0057 0.0003 0.0068 0.0104 0.001 0.0048 0.0024 0.0001 0.0014 0.0489 0.0135 0.0001
None 0.0074 0.0023 0.0021 0.0006 0.0039 0.0009 0.0042 0.0027 0.0056 0.0021 0.0035 0.0022 0.014 0.0021 0.0027 0.0044 0.003 0.0021
Nuclear Periphery 0.0061 0.0016 0.0047 0.0033 0.0079 0.0047 0.0165 0.0032 0.0048 0.0088 0.009 0.0072 0.0073 0.0009 0.002 0.0117 0.0039 0.0016
Nucleolus 0.0011 0.0001 0.0002 0 0.0041 0.0001 0.0003 0.0001 0.0006 0.0042 0.0001 0.0002 0.0003 0 0.0002 0.0003 0.0002 0
Nucleus 0.0311 0.0093 0.0218 0.0265 0.0239 0.0342 0.029 0.0148 0.0196 0.0398 0.0168 0.0288 0.0169 0.006 0.0098 0.0516 0.0111 0.0186
Peroxisomes 0.0034 0.0001 0.0039 0 0.0092 0.0001 0.006 0.0002 0.0065 0.0045 0.0007 0.0016 0.0009 0.0001 0.0064 0.002 0.0022 0.0001
Punctate Nuclear 0.0074 0.0009 0.0028 0.0003 0.0013 0.0021 0.0035 0.0037 0.0026 0.0016 0.0019 0.0033 0.0062 0.0004 0.0017 0.0069 0.0025 0.0009
Vacuole 0.0012 0.0016 0.0028 0.0006 0.0108 0.0005 0.0022 0.0008 0.0044 0.0089 0.003 0.002 0.0044 0.0002 0.001 0.0013 0.001 0.0002
Vacuole Periphery 0.0004 0.0003 0.0008 0.0002 0.0179 0.0002 0.0016 0.0001 0.0015 0.0066 0.0018 0.0008 0.0008 0 0.0002 0.0016 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.0617 2.9356 4.3567 15.9318 10.6444 39.3855 26.714 20.0335 39.7379 14.1418
Translational Efficiency 2.5001 4.6027 3.4135 2.9289 2.5191 2.5285 1.5075 2.4067 1.8321 2.4727

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (41%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rtc3

Rtc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available