Standard name
Human Ortholog
Description Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.15 0.16 0 0.05 0 0 0 0.06
Bud 0 0 0 0 0.05 0 0.06 0.05 0.05 0.05 0.1 0.05 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.13 0.15 0 0 0 0 0 0
Cytoplasm 0.23 0.35 0.67 0.71 0.6 0.46 0.41 0.37 0.36 0.36 0.3 0.37 0.88 0.9 0.93 0.06 0.06 0.09 0.1 0.09 0.13 0.15 0.18 0.15
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.09 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.16 0.07 0.05 0.13 0.08 0.06 0.06 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.28 0.49 0.52 0 0 0 0 0 0
Mitochondria 0.8 0.49 0.07 0.21 0.24 0.52 0.63 0.59 0.72 0.63 0.79 0.61 0 0 0 0.11 0.1 0.09 0.54 0.61 0.62 0.52 0.52 0.49
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.12 0.06 0.09 0.06 0.06 0.09 0.07 0.07 0.05 0.08 0 0 0 0.09 0.06 0 0 0 0 0.07 0 0.06
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.19 0.17 0.11 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 8 9 3 4 0 0 0 0 2 0 2 4 1 1 29 30 45 3 7 11 5 17 30
Bud 3 2 9 5 11 13 24 19 19 17 33 13 2 2 2 15 5 15 0 0 0 6 10 17
Bud Neck 0 0 14 4 4 3 6 6 4 6 5 5 4 2 2 0 0 0 1 1 5 3 15 13
Bud Site 0 0 0 0 1 1 4 2 7 1 3 2 0 0 0 2 0 3 – – – – – –
Cell Periphery 3 9 3 0 3 2 3 1 1 2 2 1 3 10 2 62 26 44 0 0 1 0 1 7
Cytoplasm 19 48 147 115 136 135 153 139 131 133 102 99 295 406 483 19 12 25 12 15 41 43 78 75
Endoplasmic Reticulum 3 10 4 0 5 1 0 1 0 3 0 3 4 2 5 10 4 8 1 3 1 3 5 6
Endosome 1 13 5 1 9 14 8 10 3 2 2 2 14 20 12 49 14 15 15 13 18 17 27 23
Golgi 1 0 1 1 1 0 0 0 0 0 0 0 1 0 2 87 97 148 2 2 2 3 2 5
Mitochondria 67 67 16 34 54 152 234 220 261 232 269 165 12 7 4 33 19 26 65 103 202 153 226 244
Nucleus 0 3 1 0 0 2 1 5 5 2 5 4 0 1 3 1 1 1 0 1 0 0 1 1
Nuclear Periphery 1 1 0 0 0 1 1 4 0 3 2 0 0 0 0 0 0 2 2 0 1 3 0 1
Nucleolus 0 2 0 1 0 2 2 1 3 0 1 1 1 0 0 7 3 3 0 0 1 4 0 1
Peroxisomes 1 2 2 3 2 2 2 4 0 2 0 2 3 12 5 24 5 5 4 2 6 8 5 4
SpindlePole 2 2 27 10 20 19 21 33 27 27 17 21 6 5 5 27 12 7 3 2 12 21 15 30
Vac/Vac Membrane 2 5 5 2 11 12 11 10 8 16 11 9 8 11 10 57 34 30 5 8 4 5 13 14
Unique Cell Count 84 138 218 161 225 295 374 374 361 368 340 269 335 453 521 306 196 285 121 168 324 294 435 499
Labelled Cell Count 103 172 243 179 261 359 470 455 469 448 452 329 357 479 536 422 262 377 121 168 324 294 435 499


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.1 5.2 4.5 4.8 3.9 3.7 4.1 3.4 3.7 3.2 3.6 5.2 5.4 5.6 8.8 9.5 9.6 5.3 5.4 5.7
Std Deviation (1e-4) 0.6 1.3 1.4 1.2 1.4 1.4 1.3 1.1 1.2 1.0 1.0 0.9 1.4 1.2 1.8 1.5 1.8 2.1 2.1 1.9 2.0
Intensity Change (Log2) – – – -0.21 -0.11 -0.4 -0.49 -0.31 -0.59 -0.47 -0.67 -0.51 0.01 0.06 0.12 0.77 0.89 0.9 0.04 0.07 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 -2.2 0 0 2.3 3.9 4.2
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.4 -0.9 0.6
Bud Neck 0 0 0 0 0 0 0 0 0 -3.4 -4.8 0 -4.5 -3.6 -4.3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 6.5 4.7 5.4
Cytoplasm 0.8 -1.5 -4.9 -6.2 -7.1 -7.3 -7.3 -8.7 -6.7 5.9 7.1 8.8 -14.8 -12.8 -13.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.0 0 0.7
Endosome 0 0 0 0 0 0 0 0 0 1.2 1.4 0 5.1 2.4 1.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 8.4 11.7 12.5
Mitochondria 0 0 0 0 0 0 0 0 0 -2.0 -3.9 -5.0 1.3 0.9 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 -5.1 -6.4 -7.0 -1.3 -2.2 -4.4
Vacuole 0 1.5 1.1 0.5 0.3 0 1.3 0.7 0.7 0.1 0.1 0 5.7 5.2 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3761 0.222 0.0103 -0.659 -0.2137 0.1692 0.0208 0.4848 0.4528 0.6975 0.5222 0.3279 -0.2812 -0.2294 -0.5437 -0.4055 -0.2346 -0.2757
Actin 0.0037 0.0109 0.0045 0.0031 0.0006 0.0043 0.021 0.0076 0.0052 0.0011 0.0243 0.0084 0.0181 0.0089 0.009 0.008 0.0165 0.0131
Bud 0.0083 0.0101 0.0077 0.0023 0.0015 0.0087 0.0018 0.0167 0.0095 0.01 0.0029 0.0063 0.0018 0.006 0.0039 0.0064 0.0036 0.008
Bud Neck 0.0035 0.0024 0.0013 0.0003 0.0011 0.0104 0.0017 0.0015 0.0017 0.0011 0.0045 0.0075 0.0049 0.0017 0.0021 0.0124 0.0022 0.0113
Bud Periphery 0.016 0.0114 0.0125 0.0065 0.0019 0.0074 0.006 0.0142 0.0097 0.0395 0.0069 0.0074 0.0057 0.0123 0.0113 0.014 0.0055 0.01
Bud Site 0.007 0.055 0.0155 0.0013 0.0007 0.0044 0.0039 0.0312 0.0109 0.0017 0.0108 0.003 0.0047 0.0271 0.0218 0.0035 0.0035 0.0032
Cell Periphery 0.0021 0.0024 0.0013 0.0004 0.0002 0.0008 0.0007 0.0013 0.0009 0.0006 0.0007 0.0004 0.0007 0.0016 0.0012 0.0019 0.001 0.0008
Cytoplasm 0.0759 0.1202 0.0995 0.114 0.0562 0.1105 0.0837 0.1167 0.0838 0.1448 0.1184 0.155 0.1282 0.2088 0.1397 0.3269 0.1731 0.1932
Cytoplasmic Foci 0.0926 0.0766 0.0781 0.0551 0.1526 0.1044 0.0982 0.097 0.0961 0.0375 0.1122 0.1199 0.117 0.146 0.1245 0.1209 0.1565 0.1177
Eisosomes 0.0011 0.0007 0.0007 0.0004 0.0002 0.0003 0.0004 0.0004 0.0003 0.0002 0.0006 0.0002 0.0004 0.0007 0.0005 0.0002 0.0005 0.0003
Endoplasmic Reticulum 0.0043 0.0057 0.0029 0.0035 0.0015 0.0037 0.0048 0.004 0.0033 0.0026 0.0154 0.0044 0.008 0.0052 0.007 0.0069 0.0042 0.0053
Endosome 0.0944 0.0869 0.063 0.0463 0.0927 0.0764 0.0833 0.0788 0.0604 0.013 0.0821 0.0719 0.1468 0.1285 0.1347 0.1535 0.1661 0.108
Golgi 0.0191 0.0183 0.0131 0.0142 0.0202 0.0163 0.0208 0.0163 0.0138 0.0016 0.0297 0.0102 0.0368 0.0405 0.0356 0.0305 0.0359 0.0239
Lipid Particles 0.0264 0.0083 0.0116 0.0374 0.0165 0.0178 0.0099 0.0049 0.0129 0.0011 0.0114 0.0115 0.0247 0.0052 0.0063 0.0075 0.0083 0.0091
Mitochondria 0.1145 0.0835 0.1461 0.1177 0.1557 0.1635 0.0567 0.0699 0.0907 0.0178 0.1016 0.0637 0.1412 0.1173 0.1167 0.0247 0.1847 0.1095
None 0.4654 0.4361 0.4849 0.5223 0.3758 0.4111 0.5557 0.4982 0.5637 0.6731 0.428 0.4659 0.2972 0.2453 0.3374 0.2397 0.1356 0.3374
Nuclear Periphery 0.0037 0.0037 0.0034 0.0039 0.0015 0.0029 0.0049 0.0014 0.0014 0.0042 0.0081 0.0034 0.0048 0.0014 0.0034 0.0025 0.0256 0.002
Nucleolus 0.0027 0.0058 0.0032 0.0014 0.0021 0.0023 0.0025 0.0016 0.0013 0.0027 0.0018 0.0012 0.0027 0.0019 0.001 0.0008 0.0015 0.0009
Nucleus 0.0078 0.0107 0.0116 0.0033 0.0097 0.0056 0.0081 0.0041 0.0033 0.0126 0.0035 0.0053 0.0071 0.0026 0.0029 0.0032 0.0098 0.0047
Peroxisomes 0.03 0.0197 0.0163 0.0537 0.0836 0.0281 0.0201 0.0175 0.0199 0.0041 0.0206 0.0403 0.0265 0.018 0.0191 0.0079 0.0399 0.0258
Punctate Nuclear 0.005 0.0134 0.0099 0.0045 0.0198 0.0073 0.0046 0.003 0.0037 0.0225 0.0074 0.005 0.0057 0.0049 0.0044 0.0032 0.0051 0.0039
Vacuole 0.0128 0.0149 0.0105 0.0055 0.0038 0.01 0.0088 0.011 0.0058 0.0068 0.006 0.0063 0.0131 0.0124 0.0126 0.0181 0.0119 0.0089
Vacuole Periphery 0.0037 0.0033 0.0027 0.0028 0.0022 0.0037 0.0023 0.0027 0.0018 0.0014 0.0031 0.0028 0.0039 0.0037 0.0049 0.0071 0.0089 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4762 15.3372 20.6279 12.9092 18.0738 19.2909 11.8729 14.7185 19.2927 16.4187
Translational Efficiency 0.87 0.5566 0.3365 0.8667 0.5731 0.6979 0.7855 0.6689 0.4859 0.5121

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (67%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Trr2

Trr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available