Standard name | |
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Human Ortholog | |
Description | E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | – | 3 | 0 | 1 | 1 | 4 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 |
Bud | 1 | – | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 2 | – | 3 | 1 | 8 | 2 | 13 | 12 | 20 | 10 | 3 | 12 | 1 | 2 | 0 | 5 | 1 | – | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 121 | – | 69 | 66 | 84 | 37 | 87 | 92 | 71 | 75 | 32 | 54 | 28 | 24 | 20 | 35 | 10 | – | 122 | 43 | 78 | 5 | 38 | 108 |
Endoplasmic Reticulum | 4 | – | 5 | 2 | 1 | 2 | 1 | 2 | 3 | 1 | 4 | 2 | 2 | 1 | 0 | 2 | 0 | – | 5 | 0 | 6 | 0 | 0 | 0 |
Endosome | 2 | – | 3 | 3 | 4 | 2 | 7 | 10 | 2 | 5 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | – | 0 | 0 | 2 | 0 | 3 | 2 |
Golgi | 1 | – | 4 | 2 | 3 | 1 | 4 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | – | 3 | 0 | 6 | 0 | 1 | 2 |
Mitochondria | 14 | – | 8 | 22 | 29 | 7 | 34 | 27 | 23 | 50 | 17 | 29 | 0 | 0 | 1 | 2 | 1 | – | 0 | 0 | 3 | 0 | 0 | 0 |
Nucleus | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | – | 4 | 1 | 3 | 2 | 2 | 0 | 1 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 0 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | – | 3 | 0 | 2 | 1 | 4 | 7 | 5 | 10 | 2 | 3 | 0 | 0 | 1 | 4 | 2 | – | 2 | 0 | 0 | 0 | 1 | 1 |
Unique Cell Count | 135 | 89 | 82 | 110 | 47 | 124 | 138 | 106 | 129 | 51 | 91 | 31 | 25 | 23 | 44 | 17 | 143 | 49 | 109 | 7 | 47 | 124 | ||
Labelled Cell Count | 152 | 103 | 97 | 137 | 55 | 159 | 154 | 129 | 163 | 59 | 106 | 33 | 27 | 25 | 51 | 24 | 143 | 49 | 109 | 7 | 47 | 124 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | – | 10.0 | 9.0 | 9.4 | 9.5 | 9.0 | 9.2 | 9.1 | 8.3 | 7.9 | 8.5 | 12.4 | 11.7 | 11.9 | 14.1 | 14.4 | – | – | 11.2 | 11.7 |
Std Deviation (1e-4) | 1.3 | – | 1.7 | 1.9 | 2.5 | 2.5 | 2.2 | 2.5 | 2.3 | 2.1 | 1.8 | 2.0 | 2.0 | 2.0 | 2.7 | 5.3 | 3.8 | – | – | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.08 | -0.08 | -0.15 | -0.12 | -0.14 | -0.27 | -0.34 | -0.23 | 0.3 | 0.23 | 0.25 | 0.5 | 0.53 | – | – | 0.17 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 1.9 | 1.6 | 3.3 | 1.3 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | 0.5 | -0.2 | 0.2 | -1.2 | -1.8 | -1.6 | -3.0 | -1.9 | -2.6 | 1.6 | 2.1 | 1.0 | 0.3 | -1.6 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Endosome | 0 | 0 | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Mitochondria | 3.1 | 3.1 | 1.0 | 3.3 | 2.2 | 2.4 | 4.9 | 3.6 | 3.8 | 0 | 0 | 0 | 0 | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3917 | 7.4091 | 6.6342 | 4.9326 | 5.9404 | 6.0501 | 3.3219 | 5.9464 | 5.4467 | 4.6156 | 4.468 | 4.935 | 2.9391 | 6.1932 | 4.9592 | 4.2304 | 3.658 | 4.5916 |
Actin | 0.0529 | 0.0009 | 0.0102 | 0.0003 | 0.006 | 0.0115 | 0.0469 | 0.0006 | 0.0069 | 0.0008 | 0.0008 | 0.011 | 0.0335 | 0.002 | 0.0454 | 0.0065 | 0.0139 | 0.0026 |
Bud | 0.0042 | 0.0005 | 0.001 | 0.0008 | 0.0023 | 0.0006 | 0.001 | 0.001 | 0.0009 | 0.001 | 0.0005 | 0.0007 | 0.0009 | 0.0004 | 0.0018 | 0.0017 | 0.0011 | 0.0003 |
Bud Neck | 0.0069 | 0.0007 | 0.0014 | 0.0006 | 0.0011 | 0.0013 | 0.0034 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.002 | 0.0025 | 0.0006 | 0.0009 | 0.0026 | 0.0012 | 0.0008 |
Bud Periphery | 0.0095 | 0.0003 | 0.0006 | 0.0004 | 0.0017 | 0.0004 | 0.0009 | 0.001 | 0.0006 | 0.0003 | 0.0001 | 0.0006 | 0.0009 | 0.0001 | 0.0014 | 0.001 | 0.001 | 0.0003 |
Bud Site | 0.0104 | 0.0069 | 0.0047 | 0.0006 | 0.0018 | 0.0005 | 0.0062 | 0.0024 | 0.0011 | 0.0007 | 0.0004 | 0.0014 | 0.0025 | 0.0082 | 0.0091 | 0.0559 | 0.0025 | 0.0002 |
Cell Periphery | 0.0045 | 0.0018 | 0.0008 | 0.0006 | 0.0002 | 0.0006 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.5594 | 0.8891 | 0.8268 | 0.7893 | 0.7847 | 0.807 | 0.6679 | 0.9151 | 0.8872 | 0.8095 | 0.7676 | 0.7225 | 0.5814 | 0.9263 | 0.7791 | 0.6784 | 0.7845 | 0.8443 |
Cytoplasmic Foci | 0.0456 | 0.0167 | 0.0361 | 0.0283 | 0.0264 | 0.0311 | 0.0508 | 0.0142 | 0.0172 | 0.0271 | 0.0623 | 0.0296 | 0.0403 | 0.0104 | 0.0201 | 0.0457 | 0.0327 | 0.0228 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.048 | 0.0084 | 0.0067 | 0.0097 | 0.0072 | 0.0166 | 0.0242 | 0.0031 | 0.0062 | 0.006 | 0.0047 | 0.0336 | 0.0228 | 0.0077 | 0.0112 | 0.0296 | 0.0162 | 0.0166 |
Endosome | 0.1574 | 0.0438 | 0.0719 | 0.1403 | 0.1074 | 0.0709 | 0.0737 | 0.0295 | 0.0459 | 0.1178 | 0.0956 | 0.1302 | 0.1211 | 0.0304 | 0.064 | 0.1222 | 0.06 | 0.0587 |
Golgi | 0.0309 | 0.0034 | 0.016 | 0.0024 | 0.03 | 0.0274 | 0.018 | 0.0018 | 0.0085 | 0.0048 | 0.017 | 0.033 | 0.0171 | 0.0034 | 0.0049 | 0.0081 | 0.0185 | 0.0043 |
Lipid Particles | 0.0032 | 0.0003 | 0.0017 | 0 | 0.0007 | 0.0008 | 0.0185 | 0.0012 | 0.0009 | 0.0006 | 0.027 | 0.0046 | 0.0272 | 0.0002 | 0.0028 | 0.0054 | 0.031 | 0.0066 |
Mitochondria | 0.0105 | 0.0006 | 0.0063 | 0.0004 | 0.0133 | 0.007 | 0.0083 | 0.0008 | 0.0024 | 0.0026 | 0.0019 | 0.009 | 0.0134 | 0.0007 | 0.0088 | 0.0024 | 0.0052 | 0.0036 |
None | 0.0197 | 0.0078 | 0.0018 | 0.0008 | 0.0007 | 0.0163 | 0.0167 | 0.0032 | 0.0014 | 0.0015 | 0.0015 | 0.0008 | 0.0327 | 0.001 | 0.0035 | 0.0015 | 0.0007 | 0.0013 |
Nuclear Periphery | 0.005 | 0.0012 | 0.001 | 0.0012 | 0.0021 | 0.0006 | 0.0091 | 0.0006 | 0.004 | 0.0035 | 0.0011 | 0.0023 | 0.0317 | 0.0007 | 0.002 | 0.0037 | 0.0021 | 0.0175 |
Nucleolus | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0008 | 0.0052 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0006 |
Nucleus | 0.0026 | 0.0012 | 0.0009 | 0.001 | 0.0015 | 0.0006 | 0.0046 | 0.0088 | 0.0019 | 0.0031 | 0.0011 | 0.0012 | 0.0197 | 0.0008 | 0.0014 | 0.0021 | 0.0021 | 0.0037 |
Peroxisomes | 0.0011 | 0.0001 | 0.0046 | 0.0001 | 0.0008 | 0.0011 | 0.0229 | 0.0001 | 0.0006 | 0.0004 | 0.0081 | 0.0026 | 0.0114 | 0.0001 | 0.0043 | 0.0041 | 0.0093 | 0.0035 |
Punctate Nuclear | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0077 | 0.0005 | 0.0015 | 0.0014 | 0.0012 | 0.0013 | 0.0086 | 0.0001 | 0.0008 | 0.0031 | 0.001 | 0.0048 |
Vacuole | 0.0214 | 0.0143 | 0.0054 | 0.0187 | 0.0076 | 0.0035 | 0.0148 | 0.0082 | 0.0093 | 0.0139 | 0.0062 | 0.0088 | 0.0235 | 0.0053 | 0.0317 | 0.0189 | 0.0127 | 0.0041 |
Vacuole Periphery | 0.0046 | 0.0017 | 0.0017 | 0.0044 | 0.004 | 0.0015 | 0.003 | 0.0017 | 0.0027 | 0.0043 | 0.0025 | 0.0048 | 0.0064 | 0.0013 | 0.0061 | 0.0059 | 0.0038 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.6803 | 22.3668 | 24.3425 | 21.692 | 20.5454 | 19.1718 | 21.8612 | 27.2202 | 26.2927 | 26.826 |
Translational Efficiency | 1.5314 | 1.0633 | 1.1521 | 1.297 | 1.2355 | 1.3545 | 1.5684 | 1.0838 | 1.2404 | 1.0084 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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