Standard name
Human Ortholog
Description Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.12 0.08 0.14 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0.15 0.13 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0
Golgi 0 0.05 0.09 0 0 0 0 0 – 0 0 0 0.39 0.49 0.5 0.13 0.23 0.19 0.05 0.08 0.05 0.06 0.14 0.16
Mitochondria 0.94 0.91 0.88 0.88 0.86 0.9 0.89 0.88 – 0.95 0.95 0.93 0.66 0.6 0.47 0.08 0.07 0.08 0.74 0.78 0.85 0.83 0.59 0.67
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.08 0.1 0.06 – 0.06 0.06 0.07 0 0 0 0.06 0.07 0.05 0 0 0 0 0 0
Nucleolus 0.15 0.05 0.05 0 0.1 0.13 0.16 0.13 – 0.11 0.1 0.1 0 0 0.05 0.05 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.05 0 0 0 0 – 0 0 0 0 0 0 0.12 0.1 0.07 0 0 0 0 0.06 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.11 0.07 0 0.06 0 0 0 – 0 0 0 0.26 0.19 0.28 0.21 0.25 0.22 0.09 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 2 0 0 0 0 1 – 0 0 0 1 3 0 2 5 1 0 6 6 0 2 1
Bud 0 3 2 12 0 7 6 12 – 12 11 14 1 0 0 8 6 0 0 3 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 8 2 2 – 4 13 4 0 0 0 3 2 0 – – – – – –
Cell Periphery 0 12 12 5 2 2 1 1 – 2 5 2 1 2 1 18 13 14 0 0 1 0 0 0
Cytoplasm 0 5 5 17 6 7 6 11 – 4 4 5 1 2 2 24 20 23 0 0 0 0 0 0
Endoplasmic Reticulum 0 7 3 1 0 1 2 1 – 4 1 4 3 4 1 3 12 9 0 1 0 0 0 0
Endosome 0 5 13 6 7 5 3 6 – 0 1 1 2 3 5 8 7 4 1 8 7 1 1 0
Golgi 0 17 35 1 3 0 0 0 – 0 0 0 50 73 61 20 36 19 2 28 19 1 7 7
Mitochondria 32 318 355 243 192 364 364 429 – 380 440 397 84 90 58 12 11 8 44 289 372 26 30 32
Nucleus 0 0 2 1 0 5 2 4 – 1 2 1 0 0 0 5 4 1 0 0 0 0 1 0
Nuclear Periphery 2 6 14 4 8 34 39 30 – 25 30 31 1 5 0 9 11 5 0 0 0 0 0 0
Nucleolus 5 17 20 10 22 53 64 62 – 44 46 41 5 2 6 7 7 5 0 0 0 0 0 0
Peroxisomes 0 3 25 14 4 4 1 7 – 0 4 8 0 1 0 18 16 7 0 3 18 0 3 1
SpindlePole 0 0 7 3 2 4 2 10 – 4 4 4 0 0 0 9 2 3 2 1 2 0 1 0
Vac/Vac Membrane 5 38 30 10 14 18 15 12 – 15 10 13 33 29 34 32 39 22 5 23 2 1 1 2
Unique Cell Count 34 351 403 277 224 405 407 486 398 462 425 127 150 123 155 159 102 60 371 440 32 52 49
Labelled Cell Count 44 435 525 328 260 512 507 588 495 571 525 182 214 168 178 191 121 60 371 440 32 52 49


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 11.0 9.9 9.1 8.7 7.2 6.9 6.6 – 5.8 5.9 5.7 11.5 10.9 11.7 7.8 8.7 9.2 – – –
Std Deviation (1e-4) 2.1 2.8 2.1 2.1 2.1 1.9 1.7 1.7 – 1.5 1.4 1.5 2.1 1.5 1.8 1.8 1.9 2.3 – – –
Intensity Change (Log2) – – – -0.13 -0.19 -0.46 -0.53 -0.58 – -0.77 -0.75 -0.8 0.22 0.14 0.24 -0.34 -0.19 -0.11 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 3.5 0 0 0 2.4 – 2.7 2.3 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 3.4 0 0 0 0 0 0 0
Cell Periphery -1.0 -1.7 -2.7 -3.1 -3.4 – -2.7 -2.0 -2.8 0 0 0 4.1 2.7 4.4
Cytoplasm 3.5 0 0.6 0.3 1.1 – 0 0 0 0 0 0 6.8 5.9 8.4
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome -0.8 -0.1 -1.9 -2.5 -2.0 – -3.6 -3.5 -3.3 0 0 0 1.1 0.7 0
Golgi -4.8 -3.7 -6.1 -6.1 -6.6 – -6.0 -6.5 -6.2 8.2 10.5 10.3 1.5 4.5 2.9
Mitochondria -0.1 -0.9 0.8 0.6 0.1 – 3.8 3.8 2.7 -5.7 -7.4 -9.7 -17.9 -18.2 -16.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.6 0.1 3.0 3.5 1.8 – 1.8 2.0 2.4 0 -0.1 0 1.2 1.8 0
Nucleolus -0.8 2.3 4.0 5.0 4.0 – 3.2 2.8 2.6 -0.5 -1.9 0 -0.2 -0.3 0
Peroxisomes -0.6 -2.5 -4.0 -4.8 -3.8 – -5.0 -4.4 -3.2 -2.9 -2.7 -2.8 2.1 1.6 0.2
SpindlePole 0 0 -0.9 0 0.3 – -0.9 -1.1 -1.0 0 0 0 0 0 0
Vacuole -2.1 -0.6 -1.8 -2.3 -3.5 – -2.3 -3.7 -2.8 5.6 4.0 6.0 4.4 5.6 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4205 10.1701 9.6297 9.1266 9.2702 9.6709 9.6895 11.4649 10.8356 11.4591 9.6177 10.7564 12.3982 13.7994 12.783 15.1285 11.9092 13.5379
Actin 0.0234 0.0078 0.007 0.0244 0.0072 0.0018 0.0049 0.0089 0.003 0 0.0234 0.0014 0.0065 0.0133 0.0123 0.0046 0.0023 0.0109
Bud 0.0008 0.0031 0.0012 0.0004 0.0004 0.0005 0.0002 0.0002 0.0002 0 0.0007 0 0.0046 0.0159 0.0295 0.0039 0.0081 0.0024
Bud Neck 0.0047 0.0004 0.0004 0.0001 0.0002 0.0003 0.0031 0.0001 0.0001 0 0.0001 0 0.0012 0.0005 0.0003 0.0002 0.0001 0.0524
Bud Periphery 0.0014 0.0029 0.0039 0.0008 0.001 0.0021 0.0004 0.0004 0.0009 0 0.0017 0.0001 0.0207 0.0316 0.0623 0.0199 0.013 0.0055
Bud Site 0.0132 0.0119 0.0034 0.0006 0.0007 0.0007 0.0029 0.0024 0.0004 0 0.0007 0.0001 0.0141 0.0507 0.0124 0.0006 0.0004 0.0016
Cell Periphery 0.0012 0.0015 0.0019 0.0003 0.0003 0.0018 0.0002 0.0002 0.0003 0 0.0002 0.0001 0.0088 0.0022 0.0031 0.0014 0.0005 0.0024
Cytoplasm 0.0087 0.0015 0.0099 0.0004 0.0009 0.0002 0.0003 0.0003 0 0 0.0001 0.0001 0.0915 0.0823 0.0687 0.0548 0.1337 0.0877
Cytoplasmic Foci 0.0142 0.0078 0.005 0.0028 0.005 0.0031 0.0103 0.003 0.0005 0 0.0022 0.0012 0.0198 0.0095 0.0132 0.0066 0.0147 0.0227
Eisosomes 0.0005 0.0004 0.0002 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0 0.0002 0.0001 0.0006 0.0003 0.0004 0.0022 0.0003 0.0003
Endoplasmic Reticulum 0.0033 0.0007 0.0006 0.0005 0.0023 0.0002 0.0008 0.0002 0.0001 0 0.0002 0.0003 0.0231 0.001 0.0214 0.007 0.0365 0.0296
Endosome 0.0326 0.0122 0.0093 0.0156 0.0591 0.0229 0.0222 0.0059 0.0017 0.0001 0.0048 0.0039 0.017 0.0055 0.0084 0.0097 0.0025 0.0091
Golgi 0.0154 0.0149 0.0046 0.0056 0.011 0.0338 0.0261 0.0094 0.0022 0.0003 0.013 0.0084 0.0128 0.0076 0.0049 0.0019 0.0009 0.0108
Lipid Particles 0.0229 0.0045 0.0018 0.0016 0.0084 0.0026 0.0263 0.0025 0.0041 0.0005 0.0175 0.0037 0.0125 0.0051 0.0074 0.0029 0.0017 0.0031
Mitochondria 0.7755 0.8848 0.9387 0.9167 0.8511 0.9177 0.856 0.9571 0.9786 0.9979 0.9125 0.974 0.6798 0.6867 0.701 0.7494 0.6726 0.6876
None 0.0015 0.0005 0.0002 0.0001 0.0008 0.0001 0.0002 0.0003 0 0 0.0001 0.0001 0.045 0.052 0.0288 0.0735 0.0999 0.0402
Nuclear Periphery 0.0194 0.0009 0.0006 0.0008 0.0114 0.0002 0.0018 0.0001 0.0001 0 0.0002 0.0002 0.002 0.0006 0.002 0.002 0.0014 0.0043
Nucleolus 0.0019 0.0011 0.0001 0.0005 0.0006 0.0002 0.0005 0.0001 0.0002 0 0.0003 0.0009 0.0038 0.0007 0.0014 0.0002 0.0007 0.0005
Nucleus 0.019 0.0009 0.0015 0.0003 0.0024 0.0002 0.0003 0.0001 0.0001 0 0.0002 0.0004 0.0017 0.0009 0.0012 0.0007 0.001 0.0015
Peroxisomes 0.01 0.0037 0.0019 0.0056 0.0046 0.0015 0.0332 0.0028 0.0028 0.0004 0.0081 0.0014 0.0073 0.0144 0.0031 0.0145 0.0019 0.0058
Punctate Nuclear 0.003 0.0004 0.0006 0.0001 0.001 0.0007 0.0012 0.0003 0 0 0 0.0001 0.0029 0.0005 0.0015 0.0003 0.004 0.0054
Vacuole 0.0116 0.0168 0.0039 0.009 0.0103 0.0036 0.0021 0.0015 0.002 0.0001 0.0043 0.0008 0.0149 0.0075 0.0107 0.0297 0.002 0.0066
Vacuole Periphery 0.0158 0.0213 0.0034 0.0134 0.0209 0.0055 0.0065 0.0041 0.0026 0.0005 0.0095 0.0026 0.0093 0.0112 0.0058 0.0138 0.0017 0.0098

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.3434 36.4754 36.9779 39.1362 45.8243 33.3136 40.6461 40.6681 42.9143 44.8524
Translational Efficiency 0.9283 0.7697 0.77 0.9049 0.7275 0.853 0.6945 0.8646 0.7976 0.7641

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Tom71

Tom71


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tom71-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available