Standard name | |
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Human Ortholog | |
Description | DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 5 | 4 | 4 | 8 | 10 |
Bud | 5 | 7 | 20 | 16 | 15 | 27 | 32 | 31 | 23 | 31 | 35 | 41 | 1 | 15 | 26 | 6 | 4 | 4 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 3 | 3 |
Bud Site | 0 | 1 | 3 | 0 | 5 | 1 | 17 | 9 | 5 | 8 | 15 | 8 | 0 | 1 | 2 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 2 | 4 | 2 | 3 | 0 | 1 | 1 | 0 | 3 | 2 | 3 | 0 | 1 | 7 | 16 | 9 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 12 | 20 | 34 | 37 | 34 | 67 | 78 | 25 | 49 | 35 | 36 | 24 | 59 | 118 | 15 | 6 | 16 | 0 | 0 | 3 | 4 | 1 | 2 |
Endoplasmic Reticulum | 5 | 3 | 2 | 0 | 1 | 1 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 7 | 0 | 0 | 1 | 1 | 1 | 2 |
Endosome | 40 | 7 | 7 | 2 | 39 | 6 | 9 | 11 | 0 | 1 | 1 | 0 | 17 | 30 | 20 | 53 | 36 | 38 | 32 | 23 | 25 | 18 | 28 | 30 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 16 | 29 | 13 | 5 | 2 | 7 | 5 | 12 |
Mitochondria | 128 | 142 | 161 | 218 | 224 | 335 | 442 | 488 | 311 | 368 | 376 | 414 | 94 | 117 | 178 | 18 | 28 | 17 | 144 | 126 | 146 | 100 | 132 | 112 |
Nucleus | 2 | 0 | 2 | 1 | 1 | 2 | 9 | 18 | 6 | 10 | 3 | 4 | 0 | 1 | 2 | 1 | 3 | 4 | 0 | 0 | 1 | 1 | 0 | 0 |
Nuclear Periphery | 3 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 6 | 2 | 0 | 0 | 0 | 4 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 |
Nucleolus | 3 | 1 | 0 | 1 | 1 | 1 | 2 | 10 | 4 | 5 | 2 | 5 | 0 | 0 | 1 | 2 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 19 | 7 | 24 | 13 | 42 | 10 | 17 | 19 | 4 | 5 | 10 | 3 | 9 | 28 | 7 | 43 | 30 | 21 | 13 | 10 | 16 | 9 | 14 | 14 |
SpindlePole | 15 | 4 | 18 | 10 | 38 | 15 | 43 | 65 | 18 | 21 | 32 | 33 | 6 | 5 | 7 | 38 | 34 | 32 | 3 | 3 | 5 | 2 | 7 | 6 |
Vac/Vac Membrane | 21 | 19 | 12 | 0 | 14 | 26 | 16 | 18 | 8 | 9 | 5 | 6 | 8 | 17 | 19 | 23 | 28 | 18 | 6 | 7 | 4 | 5 | 14 | 6 |
Unique Cell Count | 190 | 169 | 201 | 249 | 312 | 373 | 529 | 609 | 336 | 406 | 418 | 448 | 130 | 217 | 301 | 178 | 151 | 157 | 225 | 191 | 220 | 163 | 226 | 213 |
Labelled Cell Count | 263 | 208 | 277 | 297 | 421 | 460 | 659 | 754 | 405 | 513 | 523 | 555 | 160 | 275 | 388 | 235 | 201 | 199 | 225 | 191 | 220 | 163 | 226 | 213 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 4.5 | 4.0 | 4.2 | 4.3 | 3.8 | 3.6 | 3.7 | 3.2 | 3.1 | 3.1 | 2.8 | 4.8 | 4.8 | 4.2 | 6.3 | 6.9 | 7.5 | 5.1 | 5.3 | 5.7 |
Std Deviation (1e-4) | 1.0 | 1.3 | 1.3 | 1.8 | 1.8 | 1.7 | 1.7 | 1.7 | 1.2 | 1.4 | 1.3 | 0.8 | 1.5 | 1.9 | 1.6 | 1.2 | 1.3 | 2.3 | 2.1 | 2.3 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.13 | -0.06 | -0.13 | -0.11 | -0.33 | -0.33 | -0.37 | -0.48 | 0.27 | 0.27 | 0.1 | 0.67 | 0.8 | 0.92 | 0.35 | 0.41 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.3 | 3.7 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1248 | 1.6282 | 1.2281 | 1.2566 | 0.7328 | 1.2534 | 2.686 | 2.3851 | 2.3532 | 2.4804 | 2.1423 | 2.3743 | 2.3268 | 3.2199 | 2.5188 | 2.1071 | 2.4648 | 2.8423 |
Actin | 0.036 | 0.0401 | 0.035 | 0.0369 | 0.0037 | 0.0218 | 0.0382 | 0.0396 | 0.0383 | 0.0325 | 0.0454 | 0.0231 | 0.0293 | 0.0495 | 0.0105 | 0.0023 | 0.0245 | 0.0302 |
Bud | 0.0013 | 0.0025 | 0.0015 | 0.0009 | 0.0009 | 0.0008 | 0.0011 | 0.0022 | 0.0019 | 0.0036 | 0.002 | 0.0006 | 0.001 | 0.0015 | 0.0015 | 0.0005 | 0.0014 | 0.0008 |
Bud Neck | 0.0027 | 0.0015 | 0.0055 | 0.0017 | 0.0041 | 0.0038 | 0.0082 | 0.0015 | 0.0075 | 0.0023 | 0.0011 | 0.0045 | 0.0017 | 0.0017 | 0.0022 | 0.0013 | 0.0018 | 0.0046 |
Bud Periphery | 0.0022 | 0.0061 | 0.0034 | 0.0055 | 0.002 | 0.0015 | 0.0023 | 0.004 | 0.0042 | 0.0058 | 0.0158 | 0.0012 | 0.0036 | 0.0021 | 0.0036 | 0.0012 | 0.0041 | 0.0025 |
Bud Site | 0.0075 | 0.0098 | 0.0101 | 0.0013 | 0.0077 | 0.001 | 0.0175 | 0.0209 | 0.0137 | 0.0082 | 0.0022 | 0.0014 | 0.0046 | 0.0188 | 0.0088 | 0.0021 | 0.0022 | 0.0014 |
Cell Periphery | 0.0008 | 0.001 | 0.0039 | 0.0033 | 0.0009 | 0.0004 | 0.0007 | 0.0016 | 0.001 | 0.0005 | 0.0032 | 0.0003 | 0.0014 | 0.0008 | 0.0014 | 0.0003 | 0.0004 | 0.0004 |
Cytoplasm | 0.0061 | 0.0065 | 0.0064 | 0.0024 | 0.0048 | 0.0052 | 0.0151 | 0.0095 | 0.0137 | 0.0087 | 0.0138 | 0.0124 | 0.0132 | 0.0075 | 0.0147 | 0.0053 | 0.0047 | 0.0101 |
Cytoplasmic Foci | 0.0703 | 0.0762 | 0.0558 | 0.1005 | 0.0471 | 0.0495 | 0.1155 | 0.097 | 0.0735 | 0.0625 | 0.065 | 0.066 | 0.0715 | 0.0842 | 0.0487 | 0.0786 | 0.0934 | 0.078 |
Eisosomes | 0.0006 | 0.001 | 0.002 | 0.0008 | 0.0002 | 0.0004 | 0.0007 | 0.0011 | 0.0008 | 0.0003 | 0.0011 | 0.0004 | 0.0013 | 0.0005 | 0.001 | 0.0004 | 0.0006 | 0.0005 |
Endoplasmic Reticulum | 0.0035 | 0.0007 | 0.0046 | 0.0014 | 0.0007 | 0.0009 | 0.0042 | 0.0006 | 0.0053 | 0.0012 | 0.0023 | 0.0016 | 0.0033 | 0.001 | 0.0068 | 0.0005 | 0.0006 | 0.0008 |
Endosome | 0.1162 | 0.1421 | 0.0764 | 0.0965 | 0.1181 | 0.0694 | 0.1581 | 0.0706 | 0.0991 | 0.1503 | 0.0946 | 0.1052 | 0.1019 | 0.0568 | 0.0845 | 0.1148 | 0.0792 | 0.0809 |
Golgi | 0.1028 | 0.1371 | 0.0794 | 0.0631 | 0.0844 | 0.0815 | 0.1041 | 0.1016 | 0.0885 | 0.1009 | 0.1184 | 0.1124 | 0.0888 | 0.0779 | 0.0845 | 0.085 | 0.1086 | 0.1128 |
Lipid Particles | 0.0773 | 0.038 | 0.0398 | 0.032 | 0.0331 | 0.0306 | 0.0763 | 0.0335 | 0.037 | 0.0332 | 0.023 | 0.0314 | 0.0508 | 0.0575 | 0.0116 | 0.014 | 0.0193 | 0.0376 |
Mitochondria | 0.4535 | 0.4406 | 0.546 | 0.5396 | 0.597 | 0.6381 | 0.2828 | 0.4272 | 0.4742 | 0.453 | 0.4772 | 0.4841 | 0.4579 | 0.4807 | 0.6207 | 0.6205 | 0.4585 | 0.4753 |
None | 0.0068 | 0.0115 | 0.0098 | 0.001 | 0.0012 | 0.0066 | 0.0295 | 0.0166 | 0.0076 | 0.0024 | 0.0066 | 0.0195 | 0.0517 | 0.0346 | 0.0252 | 0.0186 | 0.0332 | 0.0118 |
Nuclear Periphery | 0.0065 | 0.0004 | 0.0011 | 0.0008 | 0.0003 | 0.0003 | 0.0123 | 0.0002 | 0.0016 | 0.001 | 0.0003 | 0.0012 | 0.0012 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0004 |
Nucleolus | 0.0023 | 0.0003 | 0.0006 | 0.0002 | 0.0012 | 0.0003 | 0.001 | 0.0013 | 0.0006 | 0.001 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0004 | 0.0005 | 0.0002 | 0.0004 |
Nucleus | 0.0045 | 0.0003 | 0.0005 | 0.0003 | 0.001 | 0.0003 | 0.0065 | 0.0004 | 0.0006 | 0.0011 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0004 |
Peroxisomes | 0.0825 | 0.0758 | 0.1093 | 0.1058 | 0.0795 | 0.0816 | 0.1104 | 0.1628 | 0.1217 | 0.1155 | 0.1222 | 0.1277 | 0.1087 | 0.1187 | 0.0667 | 0.0452 | 0.1629 | 0.1421 |
Punctate Nuclear | 0.003 | 0.0003 | 0.0005 | 0.0006 | 0.0006 | 0.0005 | 0.0062 | 0.0004 | 0.0006 | 0.0007 | 0.0004 | 0.0007 | 0.0006 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0008 |
Vacuole | 0.0087 | 0.0044 | 0.0055 | 0.0026 | 0.0066 | 0.003 | 0.0055 | 0.0058 | 0.0061 | 0.0098 | 0.0025 | 0.0028 | 0.0041 | 0.0031 | 0.0037 | 0.0032 | 0.0023 | 0.0053 |
Vacuole Periphery | 0.0047 | 0.0039 | 0.0028 | 0.0027 | 0.005 | 0.0028 | 0.0036 | 0.0019 | 0.0025 | 0.0052 | 0.0019 | 0.0028 | 0.0028 | 0.0018 | 0.0026 | 0.0047 | 0.0014 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.4938 | 9.2473 | 5.337 | 9.5173 | 11.425 | 9.9558 | 6.8393 | 5.6094 | 8.4182 | 13.2194 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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