Standard name | |
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Human Ortholog | |
Description | sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 27 | 26 | 15 | 10 | 25 | 35 | – | 42 | 26 | 55 | 11 | 1 | 6 | 3 | 13 | 12 | 0 | 1 | 18 | 14 | 25 | 26 |
Bud | 0 | 2 | 3 | 10 | 1 | 6 | 11 | 26 | – | 21 | 21 | 21 | 7 | 1 | 3 | 0 | 1 | 3 | 0 | 5 | 17 | 9 | 36 | 59 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 6 | 7 | 6 | 6 | – | 18 | 11 | 23 | 0 | 3 | 14 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Cytoplasm | 28 | 67 | 194 | 298 | 393 | 354 | 462 | 428 | – | 504 | 319 | 388 | 266 | 281 | 421 | 21 | 112 | 118 | 14 | 18 | 26 | 29 | 71 | 66 |
Endoplasmic Reticulum | 14 | 45 | 195 | 115 | 106 | 138 | 182 | 160 | – | 183 | 47 | 190 | 142 | 279 | 432 | 1 | 4 | 5 | 61 | 66 | 314 | 197 | 504 | 672 |
Endosome | 4 | 0 | 48 | 26 | 40 | 17 | 52 | 62 | – | 25 | 8 | 18 | 7 | 0 | 2 | 1 | 26 | 19 | 4 | 6 | 60 | 49 | 43 | 48 |
Golgi | 0 | 0 | 1 | 4 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 22 | 44 | 32 | 21 |
Mitochondria | 3 | 3 | 3 | 3 | 7 | 11 | 20 | 15 | – | 29 | 31 | 34 | 0 | 0 | 0 | 3 | 14 | 2 | 4 | 4 | 17 | 22 | 27 | 30 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | 2 | 1 | 0 | 1 | 2 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 17 | – | 1 | 0 | 7 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 4 | 4 | 8 | 9 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 0 | 0 | 2 | 6 | 0 | – | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 5 | 6 | 0 | 0 | 5 | 2 | 5 | 11 |
Vac/Vac Membrane | 4 | 2 | 24 | 16 | 65 | 35 | 67 | 124 | – | 60 | 23 | 42 | 27 | 14 | 17 | 1 | 17 | 17 | 11 | 22 | 29 | 37 | 75 | 92 |
Unique Cell Count | 47 | 109 | 430 | 440 | 567 | 529 | 756 | 782 | 787 | 431 | 688 | 432 | 541 | 830 | 29 | 181 | 168 | 102 | 130 | 528 | 421 | 843 | 1052 | |
Labelled Cell Count | 53 | 121 | 499 | 499 | 635 | 581 | 833 | 882 | 888 | 487 | 782 | 462 | 583 | 899 | 31 | 198 | 186 | 102 | 130 | 528 | 421 | 843 | 1052 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 8.6 | 8.8 | 8.6 | 9.1 | 8.7 | 8.7 | 8.8 | – | 8.4 | 7.9 | 8.2 | 10.7 | 10.7 | 10.8 | 6.1 | 7.7 | 10.0 | 11.5 | 11.2 | 11.6 |
Std Deviation (1e-4) | 2.8 | 2.5 | 2.4 | 2.7 | 2.6 | 2.4 | 2.3 | 2.0 | – | 2.0 | 1.6 | 1.7 | 2.3 | 2.5 | 2.3 | 1.6 | 1.9 | 2.2 | 3.0 | 2.8 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.05 | -0.01 | -0.01 | -0.0 | – | -0.06 | -0.15 | -0.1 | 0.29 | 0.29 | 0.29 | -0.53 | -0.18 | 0.19 | 0.39 | 0.35 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | -2.8 | -3.5 | -2.4 | -1.4 | – | -0.7 | -0.2 | 1.1 | -2.7 | -5.6 | -5.9 | 0 | 0.4 | 0.4 |
Bud | 1.9 | 0 | 0 | 1.2 | 2.9 | – | 2.4 | 3.7 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 2.8 | 2.9 | 3.5 | 0 | 0 | 2.3 | 0 | 0 | 0 |
Cytoplasm | 6.7 | 7.7 | 6.8 | 5.3 | 3.2 | – | 6.4 | 8.6 | 3.7 | 4.8 | 2.1 | 1.9 | 2.9 | 3.8 | 5.5 |
Endoplasmic Reticulum | -5.9 | -9.1 | -6.2 | -7.6 | -9.1 | – | -8.0 | -11.2 | -6.1 | -3.8 | 1.9 | 2.3 | -4.4 | -10.4 | -9.9 |
Endosome | -2.8 | -2.3 | -4.9 | -2.6 | -1.9 | – | -5.6 | -5.5 | -5.9 | -5.7 | -8.0 | -9.4 | 0 | 1.1 | 0.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1.8 | 2.3 | 1.7 | – | 3.1 | 4.9 | 3.9 | 0 | 0 | 0 | 0 | 4.8 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | 3.2 | 0.7 | 2.0 | 5.2 | – | 1.3 | -0.2 | 0.4 | 0.4 | -2.4 | -3.4 | 0 | 1.7 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2887 | 2.4505 | 2.2955 | 1.6568 | 1.7761 | 2.5781 | 0.3468 | 2.4837 | 1.4332 | 1.4237 | 0.3882 | 1.402 | 0.1038 | 0.878 | 0.2189 | -0.1728 | 1.2928 | 0.3846 |
Actin | 0.0079 | 0.0006 | 0.0005 | 0.0019 | 0.006 | 0.0085 | 0.0103 | 0.003 | 0.0218 | 0.0256 | 0.0027 | 0.0048 | 0.0399 | 0.0003 | 0.0057 | 0.0002 | 0.0105 | 0.0089 |
Bud | 0.002 | 0.0013 | 0.0002 | 0.0031 | 0.0245 | 0.0003 | 0.0004 | 0.0014 | 0.0015 | 0.0026 | 0.0045 | 0.0002 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0.0024 | 0.0004 |
Bud Neck | 0.0007 | 0.001 | 0.0003 | 0.0002 | 0.003 | 0.0082 | 0.0123 | 0.0004 | 0.0009 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0041 | 0.0005 |
Bud Periphery | 0.0072 | 0.0011 | 0.0002 | 0.0385 | 0.0477 | 0.0012 | 0.0006 | 0.0028 | 0.0032 | 0.0064 | 0.0085 | 0.0003 | 0.0065 | 0.0002 | 0.0002 | 0.0002 | 0.0053 | 0.0011 |
Bud Site | 0.0013 | 0.0116 | 0.0006 | 0.0003 | 0.0035 | 0.0002 | 0.0038 | 0.0052 | 0.002 | 0.0008 | 0.0021 | 0.0001 | 0.0011 | 0.0034 | 0.0005 | 0.0004 | 0.0093 | 0.0001 |
Cell Periphery | 0.0011 | 0.0006 | 0.0002 | 0.0014 | 0.0005 | 0.0002 | 0.0006 | 0.0014 | 0.0005 | 0.0006 | 0.0005 | 0.0001 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.001 | 0.0002 |
Cytoplasm | 0.1892 | 0.2612 | 0.2281 | 0.3383 | 0.0725 | 0.2362 | 0.2193 | 0.3198 | 0.2961 | 0.2281 | 0.1619 | 0.3044 | 0.0963 | 0.3309 | 0.2401 | 0.2068 | 0.2509 | 0.3055 |
Cytoplasmic Foci | 0.0309 | 0.0319 | 0.0434 | 0.0513 | 0.0455 | 0.0237 | 0.0602 | 0.0732 | 0.0521 | 0.0343 | 0.0558 | 0.0398 | 0.0711 | 0.0726 | 0.0387 | 0.0481 | 0.0427 | 0.0397 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0804 | 0.0544 | 0.0412 | 0.0139 | 0.0331 | 0.0504 | 0.0521 | 0.0763 | 0.0204 | 0.0223 | 0.0125 | 0.0245 | 0.0748 | 0.0525 | 0.0538 | 0.0156 | 0.0154 | 0.0475 |
Endosome | 0.0965 | 0.0774 | 0.1369 | 0.1262 | 0.1656 | 0.0942 | 0.1799 | 0.1488 | 0.1931 | 0.2866 | 0.2878 | 0.2296 | 0.2097 | 0.1987 | 0.2028 | 0.2224 | 0.229 | 0.1771 |
Golgi | 0.0052 | 0.0036 | 0.011 | 0.017 | 0.0054 | 0.0045 | 0.0178 | 0.1073 | 0.0267 | 0.0112 | 0.0141 | 0.014 | 0.0248 | 0.0133 | 0.0249 | 0.0048 | 0.0103 | 0.0242 |
Lipid Particles | 0.0213 | 0.0045 | 0.0035 | 0.0102 | 0.0054 | 0.0114 | 0.0225 | 0.0017 | 0.0056 | 0.002 | 0.0296 | 0.0039 | 0.0318 | 0.0139 | 0.0024 | 0.0027 | 0.0062 | 0.0174 |
Mitochondria | 0.0053 | 0.0021 | 0.0053 | 0.0095 | 0.0025 | 0.0027 | 0.016 | 0.0066 | 0.0077 | 0.0054 | 0.007 | 0.0057 | 0.0046 | 0.0019 | 0.0054 | 0.0009 | 0.0085 | 0.0038 |
None | 0.4821 | 0.5052 | 0.4813 | 0.3566 | 0.4021 | 0.4927 | 0.2971 | 0.2205 | 0.3154 | 0.3307 | 0.3534 | 0.3197 | 0.3452 | 0.2568 | 0.3779 | 0.4566 | 0.3287 | 0.308 |
Nuclear Periphery | 0.0231 | 0.0087 | 0.0096 | 0.0026 | 0.0326 | 0.0204 | 0.0134 | 0.0048 | 0.0071 | 0.0034 | 0.0077 | 0.0085 | 0.0257 | 0.0098 | 0.0103 | 0.0038 | 0.0115 | 0.0125 |
Nucleolus | 0.0007 | 0.0004 | 0.0003 | 0.0003 | 0.0046 | 0.0005 | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0046 | 0.0001 |
Nucleus | 0.0072 | 0.0035 | 0.0023 | 0.0015 | 0.035 | 0.0094 | 0.0029 | 0.001 | 0.0019 | 0.0012 | 0.0021 | 0.001 | 0.0018 | 0.0022 | 0.0016 | 0.0012 | 0.0241 | 0.0014 |
Peroxisomes | 0.0074 | 0.0007 | 0.0006 | 0.0013 | 0.003 | 0.0048 | 0.0057 | 0.0038 | 0.0018 | 0.0008 | 0.012 | 0.0023 | 0.0278 | 0.001 | 0.0005 | 0.0003 | 0.0007 | 0.0006 |
Punctate Nuclear | 0.0022 | 0.0015 | 0.0013 | 0.0012 | 0.0367 | 0.0094 | 0.0137 | 0.0005 | 0.0012 | 0.0008 | 0.0019 | 0.0008 | 0.0061 | 0.0017 | 0.0006 | 0.0011 | 0.0108 | 0.0009 |
Vacuole | 0.0241 | 0.0256 | 0.0287 | 0.021 | 0.0609 | 0.0175 | 0.0576 | 0.0168 | 0.0324 | 0.0267 | 0.0246 | 0.0293 | 0.0241 | 0.0344 | 0.0257 | 0.0295 | 0.0156 | 0.039 |
Vacuole Periphery | 0.0036 | 0.003 | 0.0044 | 0.0034 | 0.0096 | 0.0036 | 0.0122 | 0.0046 | 0.008 | 0.0097 | 0.009 | 0.0104 | 0.006 | 0.0045 | 0.008 | 0.0044 | 0.008 | 0.011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.2098 | 16.7751 | 18.6718 | 27.3538 | 21.8971 | 14.0662 | 24.3819 | 20.8921 | 25.0976 | 40.6547 |
Translational Efficiency | 1.2069 | 1.0933 | 0.8574 | 0.7212 | 1.0712 | 2.1472 | 0.9573 | 0.8161 | 0.8171 | 0.6767 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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