Standard name | |
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Human Ortholog | |
Description | Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 2 | 1 |
Bud | 1 | 0 | 1 | 0 | 3 | 4 | 4 | 7 | 9 | 7 | 7 | 8 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 3 | 2 | 1 | 0 | 2 | 7 | 1 | 3 | 3 | 2 | 2 | 1 | 2 | 14 | 11 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 164 | 60 | 182 | 190 | 283 | 198 | 249 | 309 | 157 | 181 | 114 | 139 | 286 | 268 | 383 | 261 | 250 | 278 | 162 | 74 | 166 | 185 | 215 | 186 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 13 | 15 | 26 | 0 | 0 | 0 | 3 | 0 | 0 |
Endosome | 0 | 0 | 3 | 2 | 3 | 5 | 2 | 11 | 5 | 2 | 0 | 2 | 1 | 2 | 1 | 8 | 8 | 11 | 3 | 0 | 4 | 1 | 1 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 3 | 2 | 0 | 1 | 2 | 1 | 2 |
Mitochondria | 15 | 4 | 3 | 35 | 40 | 72 | 167 | 163 | 132 | 163 | 134 | 186 | 0 | 1 | 0 | 4 | 11 | 9 | 4 | 1 | 14 | 1 | 10 | 17 |
Nucleus | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 7 | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 1 | 0 | 3 | 1 | 3 | 1 | 4 | 2 | 1 | 4 | 0 | 2 | 1 | 7 | 4 | 3 | 1 | 0 | 2 | 2 | 4 | 3 |
Vac/Vac Membrane | 2 | 0 | 1 | 2 | 1 | 0 | 7 | 8 | 8 | 5 | 9 | 8 | 2 | 4 | 0 | 8 | 0 | 12 | 6 | 0 | 4 | 3 | 4 | 8 |
Unique Cell Count | 175 | 63 | 188 | 203 | 306 | 231 | 338 | 415 | 252 | 266 | 218 | 273 | 292 | 274 | 387 | 291 | 266 | 305 | 188 | 81 | 199 | 211 | 253 | 246 |
Labelled Cell Count | 186 | 65 | 194 | 232 | 341 | 287 | 443 | 509 | 319 | 367 | 272 | 355 | 294 | 279 | 390 | 325 | 306 | 360 | 188 | 81 | 199 | 211 | 253 | 246 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 4.1 | 4.1 | 3.9 | 4.4 | 3.9 | 3.8 | 3.9 | 3.6 | 3.7 | 3.8 | 3.4 | 5.9 | 5.8 | 5.6 | 6.0 | 5.7 | 5.8 | 6.6 | 6.1 | 6.3 |
Std Deviation (1e-4) | 0.5 | 0.5 | 0.6 | 0.8 | 1.9 | 1.4 | 1.9 | 1.7 | 1.6 | 2.0 | 2.4 | 1.7 | 1.2 | 1.3 | 1.1 | 1.5 | 1.3 | 1.5 | 9.7 | 1.4 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.09 | -0.08 | -0.1 | -0.07 | -0.17 | -0.14 | -0.11 | -0.25 | 0.51 | 0.49 | 0.44 | 0.54 | 0.46 | 0.49 | 0.69 | 0.57 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4867 | 1.9392 | 2.1278 | 2.0632 | 1.7707 | 2.1425 | 0.621 | -0.0687 | -0.0153 | 0.1885 | 0.7961 | -0.0743 | -0.6751 | -0.9491 | -1.1011 | -0.6713 | -0.6273 | -0.6319 |
Actin | 0.022 | 0.0039 | 0.0095 | 0.002 | 0.008 | 0.0041 | 0.0446 | 0.0026 | 0.0029 | 0.0122 | 0.0428 | 0.006 | 0.0737 | 0.0046 | 0.0479 | 0.011 | 0.0516 | 0.0002 |
Bud | 0.0042 | 0.0098 | 0.0144 | 0.0146 | 0.0344 | 0.0059 | 0.0024 | 0.0043 | 0.0017 | 0.004 | 0.0021 | 0.001 | 0.0009 | 0.0006 | 0.0017 | 0.0003 | 0.0003 | 0 |
Bud Neck | 0.0131 | 0.0005 | 0.0008 | 0.0059 | 0.0028 | 0.0008 | 0.0032 | 0.0003 | 0.0013 | 0.0021 | 0.001 | 0.0009 | 0.0016 | 0.0002 | 0.0071 | 0.0188 | 0.001 | 0.0001 |
Bud Periphery | 0.0062 | 0.0059 | 0.011 | 0.0181 | 0.0368 | 0.01 | 0.0096 | 0.0054 | 0.0027 | 0.0095 | 0.0042 | 0.0023 | 0.0012 | 0.0003 | 0.0028 | 0.0003 | 0.0004 | 0.0001 |
Bud Site | 0.0338 | 0.0218 | 0.0175 | 0.0118 | 0.0076 | 0.0007 | 0.0113 | 0.0135 | 0.0082 | 0.0225 | 0.0057 | 0.0006 | 0.0104 | 0.0063 | 0.0075 | 0.0038 | 0.0007 | 0 |
Cell Periphery | 0.0012 | 0.0006 | 0.0006 | 0.0006 | 0.0004 | 0.0002 | 0.0006 | 0.0005 | 0.0005 | 0.001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.1207 | 0.1734 | 0.1231 | 0.1665 | 0.1018 | 0.1694 | 0.1726 | 0.3138 | 0.3946 | 0.3461 | 0.3096 | 0.479 | 0.119 | 0.1837 | 0.1818 | 0.2796 | 0.1862 | 0.3705 |
Cytoplasmic Foci | 0.0363 | 0.0155 | 0.0195 | 0.0205 | 0.063 | 0.0258 | 0.055 | 0.0079 | 0.0225 | 0.0268 | 0.025 | 0.0113 | 0.0277 | 0.0105 | 0.0156 | 0.0219 | 0.045 | 0.0131 |
Eisosomes | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0064 | 0.0018 | 0.0025 | 0.0017 | 0.0014 | 0.0016 | 0.0079 | 0.0037 | 0.0023 | 0.0132 | 0.0027 | 0.0026 | 0.0038 | 0.0017 | 0.0025 | 0.0016 | 0.0022 | 0.0032 |
Endosome | 0.0261 | 0.0039 | 0.0039 | 0.0102 | 0.0139 | 0.0127 | 0.0594 | 0.0035 | 0.0165 | 0.0301 | 0.115 | 0.0135 | 0.0162 | 0.0022 | 0.0065 | 0.0073 | 0.0285 | 0.0057 |
Golgi | 0.0102 | 0.0006 | 0.0018 | 0.0015 | 0.0152 | 0.0022 | 0.0203 | 0.0006 | 0.002 | 0.0187 | 0.0152 | 0.0047 | 0.0034 | 0.0005 | 0.0045 | 0.0078 | 0.0171 | 0.0002 |
Lipid Particles | 0.0171 | 0.0007 | 0.0053 | 0.0037 | 0.0217 | 0.0015 | 0.0278 | 0.0008 | 0.001 | 0.0086 | 0.0191 | 0.001 | 0.0077 | 0.0017 | 0.0035 | 0.0217 | 0.0286 | 0.0002 |
Mitochondria | 0.0125 | 0.001 | 0.0014 | 0.0052 | 0.012 | 0.0018 | 0.0196 | 0.0007 | 0.001 | 0.0216 | 0.0219 | 0.0085 | 0.0018 | 0.0006 | 0.0031 | 0.0012 | 0.0041 | 0.0008 |
None | 0.6126 | 0.7369 | 0.7556 | 0.6798 | 0.5877 | 0.7487 | 0.4702 | 0.6342 | 0.5184 | 0.4568 | 0.3932 | 0.4612 | 0.6263 | 0.7836 | 0.7023 | 0.6131 | 0.5464 | 0.565 |
Nuclear Periphery | 0.0122 | 0.0012 | 0.0014 | 0.0046 | 0.003 | 0.0024 | 0.0315 | 0.001 | 0.0014 | 0.003 | 0.0232 | 0.0016 | 0.0236 | 0.0004 | 0.0016 | 0.0007 | 0.0047 | 0.0292 |
Nucleolus | 0.0056 | 0.0011 | 0.0029 | 0.0058 | 0.0057 | 0.0006 | 0.0039 | 0.0005 | 0.0004 | 0.0015 | 0.0004 | 0.0002 | 0.0039 | 0.0002 | 0.0011 | 0.0002 | 0.0004 | 0.0002 |
Nucleus | 0.0175 | 0.0053 | 0.0049 | 0.0135 | 0.0176 | 0.0026 | 0.0086 | 0.0018 | 0.0024 | 0.0028 | 0.0023 | 0.0015 | 0.0558 | 0.0005 | 0.003 | 0.0003 | 0.0011 | 0.0059 |
Peroxisomes | 0.0153 | 0.0012 | 0.0116 | 0.0138 | 0.0235 | 0.002 | 0.0144 | 0.0004 | 0.0039 | 0.0029 | 0.0035 | 0.0005 | 0.0051 | 0.0004 | 0.003 | 0.0071 | 0.0636 | 0.0001 |
Punctate Nuclear | 0.0119 | 0.0125 | 0.0101 | 0.0153 | 0.038 | 0.0044 | 0.0243 | 0.0016 | 0.0054 | 0.0029 | 0.0035 | 0.0012 | 0.0137 | 0.0011 | 0.0018 | 0.0012 | 0.0166 | 0.0012 |
Vacuole | 0.0114 | 0.0018 | 0.0017 | 0.0035 | 0.0045 | 0.0017 | 0.0087 | 0.0021 | 0.0093 | 0.0101 | 0.0041 | 0.0017 | 0.0025 | 0.0007 | 0.0014 | 0.0012 | 0.0006 | 0.0016 |
Vacuole Periphery | 0.0032 | 0.0002 | 0.0003 | 0.001 | 0.0008 | 0.0007 | 0.0037 | 0.0003 | 0.0014 | 0.0036 | 0.0044 | 0.0007 | 0.0009 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.8969 | 13.6641 | 8.2382 | 11.1576 | 13.6249 | 10.5028 | 10.3618 | 13.4042 | 9.7348 | 14.4244 |
Translational Efficiency | 0.5885 | 0.4147 | 0.5144 | 0.4435 | 0.4195 | 0.6766 | 0.5157 | 0.3878 | 0.3577 | 0.3332 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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