Standard name | |
---|---|
Human Ortholog | |
Description | Putative metalloprotease with similarity to zinc carboxypeptidases; required for normal cell wall assembly |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 1 | 7 | 2 | 1 | 1 | 4 | 0 | 6 | 4 | 5 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 14 | 30 | 54 | 84 | 204 | 315 | 344 | 327 | 365 | 370 | 463 | 55 | 73 | 153 | 34 | 28 | 29 | 1 | 1 | 1 | 8 | 11 | 3 |
Endoplasmic Reticulum | 1 | 4 | 0 | 0 | 1 | 4 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 1 | 5 | 7 | 5 | 6 | 2 | 0 | 1 | 1 | 2 | 2 |
Endosome | 20 | 4 | 10 | 5 | 9 | 2 | 0 | 7 | 1 | 0 | 0 | 2 | 19 | 20 | 15 | 4 | 7 | 5 | 9 | 15 | 15 | 18 | 25 | 28 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 1 |
Mitochondria | 44 | 33 | 174 | 35 | 60 | 137 | 139 | 87 | 87 | 124 | 142 | 144 | 2 | 0 | 2 | 6 | 5 | 2 | 7 | 4 | 9 | 9 | 11 | 10 |
Nucleus | 39 | 42 | 143 | 127 | 99 | 117 | 97 | 81 | 52 | 58 | 32 | 55 | 50 | 122 | 142 | 75 | 42 | 37 | 6 | 3 | 8 | 8 | 17 | 7 |
Nuclear Periphery | 4 | 0 | 3 | 1 | 4 | 19 | 7 | 12 | 4 | 15 | 6 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 8 | 3 | 4 | 11 |
Nucleolus | 10 | 1 | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | 2 | 0 | 0 | 5 | 0 | 3 | 3 | 4 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 3 | 2 |
Vac/Vac Membrane | 122 | 173 | 226 | 288 | 306 | 359 | 420 | 439 | 337 | 379 | 326 | 379 | 177 | 252 | 287 | 86 | 86 | 76 | 216 | 200 | 398 | 293 | 303 | 313 |
Unique Cell Count | 206 | 228 | 453 | 388 | 416 | 584 | 735 | 737 | 617 | 699 | 658 | 809 | 258 | 368 | 452 | 161 | 134 | 127 | 259 | 237 | 463 | 357 | 394 | 397 |
Labelled Cell Count | 254 | 273 | 602 | 514 | 564 | 844 | 982 | 971 | 816 | 946 | 882 | 1060 | 303 | 469 | 608 | 217 | 174 | 156 | 259 | 237 | 463 | 357 | 394 | 397 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 6.0 | 4.8 | 5.4 | 5.8 | 4.9 | 4.9 | 4.9 | 4.7 | 4.4 | 4.2 | 4.4 | 6.4 | 6.3 | 6.1 | 6.8 | 7.2 | 6.9 | 7.0 | 7.0 | 7.5 |
Std Deviation (1e-4) | 0.9 | 1.1 | 1.1 | 1.2 | 1.7 | 1.5 | 1.5 | 1.5 | 1.3 | 1.1 | 1.3 | 1.1 | 1.1 | 1.0 | 1.1 | 1.7 | 2.0 | 2.0 | 1.4 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.29 | 0.05 | 0.03 | 0.05 | -0.03 | -0.13 | -0.19 | -0.13 | 0.42 | 0.4 | 0.35 | 0.51 | 0.6 | 0.53 | 0.55 | 0.56 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.5 | 5.9 | 10.8 | 13.4 | 14.5 | 15.9 | 15.9 | 16.9 | 17.7 | 5.8 | 5.7 | 10.2 | 5.2 | 4.9 | 5.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.0 | 0 | -2.8 | 0 | -1.8 | 0 | 0 | 0 | 0 | 3.3 | 2.5 | 1.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -9.8 | -8.0 | -5.2 | 0 | -10.8 | 0 | 0 | 0 | 0 | -11.2 | -13.4 | -14.4 | -8.3 | -7.7 | -8.0 |
Nucleus | 0.4 | -2.6 | -4.3 | -7.7 | -8.8 | -9.7 | -10.2 | -12.0 | -11.6 | -3.5 | 0.5 | 0 | 3.4 | 0 | -0.5 |
Nuclear Periphery | 0 | 0 | 2.9 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 7.2 | 7.2 | 3.7 | 2.4 | 3.3 | 1.5 | 1.4 | -0.1 | -1.0 | 4.8 | 5.4 | 4.1 | 0.8 | 2.9 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9628 | 4.4618 | 3.9305 | 3.9867 | 3.0901 | 4.2424 | 0.7225 | 1.5709 | 1.6675 | 1.0691 | 1.4692 | 1.4652 | 0.9049 | 1.6339 | 1.416 | 1.525 | 1.8129 | 1.3937 |
Actin | 0.0106 | 0.0001 | 0.0002 | 0.0008 | 0 | 0.0003 | 0.0316 | 0.0005 | 0.0024 | 0 | 0 | 0.0001 | 0.0107 | 0.0001 | 0.0013 | 0.0006 | 0.0108 | 0.0032 |
Bud | 0.0005 | 0.0005 | 0.0047 | 0.0004 | 0.0006 | 0.0005 | 0.0027 | 0.0005 | 0.0008 | 0.0005 | 0.0002 | 0.0003 | 0.0063 | 0.005 | 0.0101 | 0.0029 | 0.0005 | 0.0014 |
Bud Neck | 0.0024 | 0.0035 | 0.001 | 0.002 | 0.0019 | 0.0053 | 0.0169 | 0.0024 | 0.0014 | 0.0019 | 0.0006 | 0.0017 | 0.0016 | 0.0029 | 0.0025 | 0.0008 | 0.0021 | 0.0036 |
Bud Periphery | 0.0013 | 0.0017 | 0.004 | 0.0011 | 0.0016 | 0.0017 | 0.0058 | 0.0013 | 0.0016 | 0.0011 | 0.0003 | 0.0008 | 0.0047 | 0.0028 | 0.0048 | 0.0075 | 0.0013 | 0.0021 |
Bud Site | 0.0025 | 0.0031 | 0.0028 | 0.0013 | 0.0023 | 0.0014 | 0.0318 | 0.0021 | 0.006 | 0.0022 | 0.0006 | 0.0007 | 0.0126 | 0.0177 | 0.0058 | 0.0007 | 0.0013 | 0.0015 |
Cell Periphery | 0.0034 | 0.0056 | 0.0037 | 0.003 | 0.0025 | 0.0041 | 0.0035 | 0.002 | 0.0019 | 0.0019 | 0.0009 | 0.0034 | 0.0017 | 0.0037 | 0.0024 | 0.0015 | 0.0022 | 0.0039 |
Cytoplasm | 0.0109 | 0.0225 | 0.004 | 0.0022 | 0.0035 | 0.0068 | 0.0123 | 0.0121 | 0.0123 | 0.0039 | 0.0011 | 0.0164 | 0.0066 | 0.0066 | 0.0147 | 0.0035 | 0.0019 | 0.0064 |
Cytoplasmic Foci | 0.021 | 0.0086 | 0.0237 | 0.0202 | 0.0076 | 0.0178 | 0.0378 | 0.01 | 0.0255 | 0.004 | 0.0045 | 0.0111 | 0.0241 | 0.0285 | 0.0198 | 0.0464 | 0.0135 | 0.0416 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0038 | 0.0041 | 0.0051 | 0.0015 | 0.0017 | 0.0025 | 0.005 | 0.0039 | 0.007 | 0.0017 | 0.0008 | 0.0024 | 0.0038 | 0.0043 | 0.0033 | 0.0015 | 0.0019 | 0.0032 |
Endosome | 0.2339 | 0.1267 | 0.1783 | 0.1283 | 0.1531 | 0.1452 | 0.2536 | 0.1604 | 0.2914 | 0.2001 | 0.2524 | 0.1421 | 0.2986 | 0.1782 | 0.2343 | 0.2124 | 0.2686 | 0.1901 |
Golgi | 0.0134 | 0.0047 | 0.0073 | 0.0119 | 0.0033 | 0.005 | 0.0224 | 0.0056 | 0.0238 | 0.0063 | 0.0063 | 0.0052 | 0.0187 | 0.0044 | 0.0137 | 0.0066 | 0.0317 | 0.0066 |
Lipid Particles | 0.0547 | 0.0194 | 0.0354 | 0.0244 | 0.0241 | 0.0295 | 0.0373 | 0.0125 | 0.0154 | 0.0049 | 0.0082 | 0.0268 | 0.0405 | 0.0085 | 0.0248 | 0.0304 | 0.0261 | 0.0177 |
Mitochondria | 0.045 | 0.0152 | 0.0748 | 0.0157 | 0.0035 | 0.0144 | 0.0222 | 0.0151 | 0.0448 | 0.0021 | 0.0077 | 0.0055 | 0.0456 | 0.0243 | 0.1012 | 0.0139 | 0.0346 | 0.0147 |
None | 0.0134 | 0.013 | 0.0049 | 0.0003 | 0.0028 | 0.0055 | 0.0459 | 0.0157 | 0.0125 | 0.0002 | 0.0001 | 0.0004 | 0.0015 | 0.0019 | 0.0124 | 0.0006 | 0.0003 | 0.0029 |
Nuclear Periphery | 0.0056 | 0.0053 | 0.0072 | 0.0016 | 0.0021 | 0.0073 | 0.0122 | 0.0128 | 0.0292 | 0.0026 | 0.0022 | 0.0032 | 0.0151 | 0.0127 | 0.009 | 0.0026 | 0.0018 | 0.0074 |
Nucleolus | 0.0089 | 0.0133 | 0.0071 | 0.004 | 0.012 | 0.0122 | 0.0076 | 0.0047 | 0.007 | 0.0045 | 0.0023 | 0.0045 | 0.0081 | 0.0127 | 0.0081 | 0.0184 | 0.0051 | 0.0111 |
Nucleus | 0.018 | 0.0075 | 0.0061 | 0.0036 | 0.0056 | 0.0117 | 0.0095 | 0.0098 | 0.0176 | 0.0034 | 0.0015 | 0.0036 | 0.0091 | 0.0119 | 0.0108 | 0.0026 | 0.0028 | 0.0105 |
Peroxisomes | 0.0062 | 0.0023 | 0.0073 | 0.0009 | 0.0034 | 0.0031 | 0.012 | 0.0006 | 0.0039 | 0.0005 | 0.0007 | 0.0013 | 0.0077 | 0.0014 | 0.0043 | 0.0026 | 0.0025 | 0.0029 |
Punctate Nuclear | 0.0034 | 0.0004 | 0.0029 | 0.0001 | 0.0012 | 0.0019 | 0.0071 | 0.0005 | 0.0049 | 0.0001 | 0.0001 | 0.0002 | 0.0034 | 0.0044 | 0.0021 | 0.0028 | 0.0002 | 0.0041 |
Vacuole | 0.4195 | 0.6271 | 0.4777 | 0.6546 | 0.6877 | 0.6011 | 0.3357 | 0.5864 | 0.3779 | 0.6554 | 0.5874 | 0.6471 | 0.3586 | 0.525 | 0.37 | 0.474 | 0.4868 | 0.5349 |
Vacuole Periphery | 0.1214 | 0.1155 | 0.1418 | 0.1219 | 0.0794 | 0.1227 | 0.0857 | 0.1412 | 0.1128 | 0.1027 | 0.1222 | 0.1231 | 0.121 | 0.143 | 0.1445 | 0.1675 | 0.1039 | 0.1301 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.8692 | 53.4001 | 48.6824 | 62.5991 | 45.3636 | 47.7621 | 56.1139 | 65.3344 | 59.5122 | 60.7041 |
Translational Efficiency | 1.2925 | 1.235 | 1.5284 | 0.9041 | 1.2462 | 1.7166 | 1.3041 | 0.9149 | 1.0993 | 0.995 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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