Standard name
Human Ortholog
Description Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.96 0.95 0.93 0.95 0.9 – 0.81 0.82 0.8 0.68 0.74 0.97 0.95 0.96 0.68 0.67 0.69 0.54 0.7 0.64 0.55 0.56 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0.1 0 0.09 0.09 0.09 0.08
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0.07 0.05 0 0 0 0.06 0
Mitochondria 0 0 0 0 0 0.18 – 0.13 0.24 0.22 0.33 0.3 0 0 0 0 0 0 0 0.05 0.07 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 – 0.06 0.05 0 0.05 0 0 0 0 0.28 0.4 0.24 0.14 0.1 0.05 0.17 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 2 1 2 1 – 3 1 5 0 4 0 1 1 2 0 1 4 1 3 1 6 4
Bud 2 0 3 4 7 5 – 18 18 24 26 40 0 2 1 0 1 2 7 0 2 2 14 11
Bud Neck 0 0 0 2 8 2 – 10 5 6 0 5 0 0 2 0 0 0 1 0 0 1 0 0
Bud Site 0 0 0 1 0 0 – 2 2 4 3 1 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 – 1 0 3 0 0 1 1 0 1 1 2 0 0 0 0 0 0
Cytoplasm 191 65 91 286 466 279 – 476 343 423 248 465 165 348 447 136 39 93 128 63 57 38 136 158
Endoplasmic Reticulum 3 0 0 2 1 1 – 0 0 1 1 2 2 4 5 7 4 9 1 0 0 0 2 2
Endosome 2 1 1 5 1 1 – 13 2 13 6 5 1 2 2 9 2 6 24 3 8 6 21 19
Golgi 8 2 0 1 1 0 – 0 0 0 0 1 1 0 7 12 2 9 12 0 0 3 15 7
Mitochondria 5 0 4 8 11 55 – 74 100 114 121 189 2 4 2 5 2 3 9 4 6 2 9 8
Nucleus 0 0 0 1 3 2 – 11 2 5 3 4 0 0 0 1 0 1 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 – 4 2 4 6 6 0 0 0 0 0 0 2 0 0 0 0 1
Nucleolus 3 1 0 2 0 1 – 6 2 3 6 6 0 2 1 1 0 4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 1 1 4 0 0 0 0 4 0 0 0 0 0
SpindlePole 0 0 0 3 1 1 – 12 2 7 4 12 1 5 3 0 0 0 3 1 1 0 4 5
Vac/Vac Membrane 13 2 1 10 15 6 – 33 19 17 18 22 1 3 6 55 23 32 32 9 4 11 26 31
Unique Cell Count 216 68 96 306 491 310 589 416 528 365 626 170 365 468 199 58 134 239 91 89 70 244 255
Labelled Cell Count 229 72 102 326 517 355 663 498 629 442 763 175 377 477 229 74 162 239 91 89 70 244 255


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 6.9 5.5 5.5 5.3 4.1 – 4.8 4.0 4.0 4.4 4.2 9.3 11.6 8.9 18.4 21.6 20.2 11.0 9.2 7.8
Std Deviation (1e-4) 11.3 7.9 5.5 5.8 4.3 3.1 – 3.5 2.4 1.9 2.5 2.3 11.5 13.1 9.7 14.9 17.0 16.5 10.2 9.5 7.6
Intensity Change (Log2) – – – -0.01 -0.08 -0.43 – -0.21 -0.46 -0.47 -0.34 -0.42 0.74 1.07 0.69 1.73 1.96 1.87 0.98 0.74 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 0 -1.4 – -3.4 -3.0 -3.5 -5.3 -4.4 0.9 0.2 0.3 -5.1 -4.6 -4.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 5.5 6.4 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2616 -0.6013 -0.4018 -0.6185 -0.1678 -0.0516 0.1141 -0.2121 -0.1865 0.855 -1.5438 0.4466 0.5646 2.2462 1.589 1.7931 1.6495 1.7445
Actin 0.0245 0.0042 0.0108 0.0322 0.0135 0.0036 0.0385 0.003 0.0094 0.0002 0.0017 0.0018 0.0002 0.0001 0.0006 0.0014 0.0007 0.0006
Bud 0.001 0.0028 0.0008 0.0097 0.0134 0.0009 0.0013 0.0001 0.0012 0.0001 0.0005 0.0004 0.0009 0.0082 0.0089 0.0024 0.0023 0.0028
Bud Neck 0.0015 0.0001 0.0035 0.0028 0.0015 0.0023 0.0122 0.0001 0.0009 0.0001 0.0024 0.0004 0.0001 0.0001 0.0002 0.0004 0.0002 0.0002
Bud Periphery 0.002 0.0057 0.0007 0.0056 0.0099 0.0011 0.0015 0.0001 0.0021 0.0001 0.0007 0.0004 0.0004 0.0022 0.0057 0.0027 0.0012 0.0029
Bud Site 0.0063 0.004 0.0094 0.003 0.0073 0.0005 0.0161 0.0003 0.0163 0.0001 0.0134 0.0002 0.0001 0.0023 0.0033 0.0009 0.0001 0.0002
Cell Periphery 0.0002 0.0001 0.0004 0.0003 0.0003 0.0001 0.0006 0.0001 0.0003 0 0.0005 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002
Cytoplasm 0.0384 0.0434 0.0462 0.0242 0.1098 0.0817 0.0756 0.1368 0.0687 0.1364 0.0267 0.1179 0.0426 0.113 0.0905 0.0669 0.0821 0.1644
Cytoplasmic Foci 0.0215 0.0064 0.0101 0.0093 0.0203 0.0122 0.0872 0.0215 0.0232 0.0359 0.0259 0.0598 0.0109 0.0123 0.0155 0.0341 0.0382 0.0175
Eisosomes 0.0004 0.0002 0.0005 0.0002 0.0002 0.0001 0.0005 0.001 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0021 0.0012 0.0009 0.0011 0.0036 0.0008 0.0068 0.0006 0.0008 0.0021 0.0003 0.0007 0.0005 0.0006 0.0007 0.0008 0.0005 0.0009
Endosome 0.012 0.0028 0.0031 0.0069 0.041 0.0127 0.0635 0.0072 0.0125 0.0404 0.0221 0.0106 0.02 0.0148 0.0167 0.0089 0.0336 0.0075
Golgi 0.0081 0.0003 0.0015 0.0028 0.0086 0.0017 0.021 0.0011 0.0073 0.0412 0.0134 0.0035 0.0035 0.0018 0.0017 0.0033 0.0032 0.0009
Lipid Particles 0.0122 0.0007 0.0028 0.0019 0.0475 0.0049 0.0305 0.0006 0.0013 0.0003 0.0358 0.021 0.001 0.0004 0.0005 0.0007 0.0003 0.0004
Mitochondria 0.0056 0.0007 0.0051 0.006 0.0069 0.0024 0.0163 0.0004 0.0031 0.0002 0.0227 0.0168 0.0002 0.0003 0.0008 0.0008 0.0004 0.0005
None 0.8181 0.9137 0.8887 0.8765 0.6512 0.8597 0.5415 0.796 0.8402 0.7374 0.8053 0.7424 0.9009 0.8407 0.8386 0.8499 0.8291 0.7913
Nuclear Periphery 0.0071 0.0013 0.0011 0.0032 0.0145 0.002 0.0309 0.0013 0.0013 0.0009 0.0002 0.001 0.0017 0.0003 0.0011 0.0018 0.0004 0.0009
Nucleolus 0.0014 0.0004 0.0011 0.0019 0.0032 0.0004 0.0013 0.0003 0.0007 0 0.0027 0.0076 0.0001 0.0001 0.0006 0.003 0.0001 0.0001
Nucleus 0.0085 0.0008 0.0013 0.0041 0.0077 0.0016 0.0076 0.0133 0.0009 0.0005 0.0009 0.0022 0.0043 0.0006 0.003 0.0054 0.001 0.0017
Peroxisomes 0.0155 0.0015 0.0012 0.0021 0.0173 0.0032 0.0216 0.0024 0.0019 0.0003 0.0185 0.0043 0.0015 0.0004 0.0011 0.0049 0.0047 0.0012
Punctate Nuclear 0.0114 0.0016 0.0054 0.0018 0.0148 0.0024 0.0173 0.0011 0.0006 0.0004 0.0008 0.005 0.0023 0.0002 0.0017 0.0086 0.0009 0.0007
Vacuole 0.0013 0.0075 0.005 0.003 0.0052 0.0044 0.0058 0.0122 0.0048 0.003 0.0045 0.0019 0.0057 0.0009 0.007 0.002 0.0006 0.0042
Vacuole Periphery 0.001 0.0005 0.0005 0.0012 0.0021 0.0013 0.0023 0.0005 0.0023 0.0005 0.0009 0.0021 0.003 0.0002 0.0015 0.0008 0.0002 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.4504 26.4799 55.6728 70.3011 27.204 51.8091 68.0501 84.329 90.0033 74.6733
Translational Efficiency 1.5279 2.426 1.9758 1.5424 2.823 1.2973 1.8271 2.5022 2.014 2.1357

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (16%), vacuole (7%), mixed (50%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00022)
vacuole - S/G2 (0.00076)
Subcompartmental Group N/A

Spl2

Spl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available