ORF
Human Ortholog
Description Putative integral membrane protein of unknown function

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.06 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.94 0.91 0.94 0.92 0.9 0.84 0.8 0.8 0.83 0.8 0.99 0.96 0.94 0.92 0.88 0.94 0.43 0.44 0.37 0.51 0.53 0.59
Endoplasmic Reticulum 0.12 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0.05 0.05 0 0.18 0.19 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.1 0.07 0.07 0.14 0.17 0.17 0.16 0.2 0.22 0 0 0 0.05 0 0 0.15 0 0 0.06 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.1 0.06 0.25 0.25 0.36 0.35 0.32 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 3 0 3 0 2 1 0 1 0 0 2 0 1 3 4
Bud 0 1 0 4 9 20 16 24 12 23 35 4 1 0 1 1 0 0 2 0 1 8 12
Bud Neck 1 0 0 0 0 0 0 1 0 1 2 2 1 5 0 0 2 0 0 1 2 5 4
Bud Site 0 0 0 0 0 0 1 2 1 1 2 0 0 0 0 0 0
Cell Periphery 0 0 0 2 1 1 1 1 5 0 5 5 3 9 0 1 1 0 0 0 0 1 2
Cytoplasm 103 124 99 304 280 482 451 339 384 408 481 362 464 549 269 203 169 1 56 52 132 231 294
Endoplasmic Reticulum 14 3 1 6 7 7 4 20 14 14 25 5 15 30 12 12 9 0 22 26 7 8 6
Endosome 0 0 0 1 1 1 3 1 2 0 1 0 0 0 2 5 0 0 2 3 1 5 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 3 2
Mitochondria 1 7 11 21 20 75 93 74 78 100 132 0 0 0 15 5 0 0 4 4 15 19 26
Nucleus 2 0 1 0 0 4 7 2 4 3 1 1 0 0 1 3 1 0 0 0 1 0 1
Nuclear Periphery 0 0 2 0 0 1 8 1 3 3 1 0 0 1 0 0 1 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 1 0 1 1 2 0 1 0 2 0 0 0 0 0 2 5 8
Vac/Vac Membrane 0 4 2 4 5 13 19 13 27 21 21 2 0 4 13 22 11 1 32 51 90 138 116
Unique Cell Count 120 132 109 323 304 536 535 423 478 491 601 367 483 583 291 230 179 4 129 143 261 437 500
Labelled Cell Count 121 139 116 345 323 606 604 479 534 575 711 381 488 599 316 253 194 4 129 143 261 437 500


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.0 3.7 4.4 3.9 3.6 4.0 3.9 4.1 4.1 4.2 5.2 5.2 5.4 4.9 5.1 5.2 5.8 5.6 5.6
Std Deviation (1e-4) 1.0 0.7 1.1 1.5 0.9 0.9 1.3 0.9 1.0 1.1 1.2 0.8 0.8 0.9 0.9 1.1 1.1 1.3 1.6 1.4
Intensity Change (Log2) -0.11 0.12 -0.03 -0.14 0.01 -0.03 0.03 0.01 0.07 0.37 0.37 0.42 0.29 0.36 0.36 0.53 0.49 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 0.1 -0.7 -1.4 -2.9 -3.7 -3.7 -3.1 -3.8 2.9 1.1 0.1 -0.6 -1.8 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1.4 0 1.4 1.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -0.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 1.2 0.6 0 0 0 0 0.7 2.3 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4437 -0.1555 -0.3532 0.3252 -0.4295 -0.2055 -0.4444 0.645 0.0533 -0.042 -0.7458 -0.1857 -2.0964 -1.285 -1.7258 -1.6167 -2.2786 -1.9014
Actin 0.0175 0.0003 0.0055 0.0266 0.0244 0.0017 0.0215 0.0001 0.001 0 0.0095 0.0004 0.0061 0.0005 0.0004 0.0047 0.0003 0.0002
Bud 0.0002 0.0004 0.0032 0.0002 0.0002 0.0009 0.0018 0.0001 0.0001 0 0.0025 0.0001 0.0002 0 0.0001 0.0032 0 0
Bud Neck 0.001 0.0002 0.0008 0.0004 0.0024 0.002 0.0008 0 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0006 0.0001 0.0002
Bud Periphery 0.0002 0.0005 0.0032 0.0002 0.0003 0.0037 0.003 0.0001 0.0002 0.0001 0.0128 0.0002 0.0004 0 0.0001 0.0081 0 0
Bud Site 0.0038 0.002 0.0117 0.0003 0.0003 0.0002 0.0038 0.0002 0.0002 0.0001 0.0003 0 0.001 0.0002 0.0002 0.0008 0.0001 0
Cell Periphery 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0005 0.0001 0.0003 0.0001 0.0006 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0
Cytoplasm 0.0949 0.1057 0.1042 0.0898 0.1881 0.1483 0.0652 0.162 0.1041 0.1165 0.1039 0.1391 0.1701 0.2369 0.1953 0.2351 0.2999 0.349
Cytoplasmic Foci 0.006 0.005 0.0042 0.014 0.004 0.009 0.0188 0.0104 0.0075 0.0455 0.0317 0.0112 0.0097 0.012 0.0169 0.0194 0.0147 0.0209
Eisosomes 0.0002 0.0002 0.0003 0.0002 0.0002 0 0.0006 0.0002 0.0005 0.0001 0.0003 0.0001 0.0003 0.0005 0.0002 0.0004 0 0.0001
Endoplasmic Reticulum 0.011 0.003 0.0109 0.0053 0.0043 0.0121 0.0055 0.0073 0.0042 0.0016 0.0071 0.0038 0.0121 0.0089 0.0095 0.0091 0.0028 0.0055
Endosome 0.0037 0.0025 0.0042 0.0161 0.0043 0.0052 0.0131 0.0234 0.0094 0.0447 0.0556 0.0113 0.006 0.0096 0.0059 0.0402 0.0041 0.013
Golgi 0.0007 0.0002 0.0004 0.0023 0.0018 0.0004 0.0017 0.0003 0.0005 0.0005 0.0011 0.0001 0.001 0.0002 0.0003 0.0009 0.0002 0.0002
Lipid Particles 0.0012 0.0006 0.0008 0.0022 0.0018 0.0005 0.0041 0.0007 0.0023 0.002 0.0218 0.0005 0.0028 0.0012 0.0033 0.0038 0.0007 0.002
Mitochondria 0.0005 0.0004 0.0008 0.002 0.0007 0.0004 0.0014 0.0005 0.0023 0.0009 0.0034 0.0003 0.0007 0.0002 0.0005 0.0019 0.0005 0.0002
None 0.8428 0.8676 0.8303 0.7978 0.7523 0.7992 0.833 0.7775 0.8376 0.7688 0.7207 0.815 0.778 0.7218 0.7552 0.6458 0.6726 0.6001
Nuclear Periphery 0.003 0.0015 0.0039 0.0247 0.0048 0.0061 0.0037 0.0019 0.0023 0.0008 0.0106 0.0006 0.0024 0.0024 0.0022 0.0064 0.0008 0.0013
Nucleolus 0.002 0.0006 0.001 0.0005 0.0006 0.0003 0.0006 0.0002 0.0005 0.0004 0.0004 0.0001 0.0005 0.0001 0.0003 0.0005 0.0001 0.0001
Nucleus 0.0027 0.0036 0.0039 0.0044 0.0018 0.0048 0.0021 0.0012 0.0013 0.0012 0.0023 0.0008 0.0025 0.0017 0.0014 0.0036 0.0008 0.0017
Peroxisomes 0.0005 0.0001 0.0002 0.0013 0.0005 0.0002 0.0042 0.0001 0.0006 0.0002 0.0005 0.0003 0.0006 0.0002 0.0027 0.0004 0.0002 0.0002
Punctate Nuclear 0.0066 0.0038 0.0021 0.0092 0.0058 0.0035 0.0064 0.0003 0.0023 0.0007 0.0008 0.0006 0.0024 0.0006 0.0023 0.0028 0.0007 0.0016
Vacuole 0.001 0.0013 0.0078 0.0017 0.001 0.0011 0.0076 0.0116 0.0176 0.0143 0.012 0.0137 0.0025 0.0025 0.0028 0.0089 0.0011 0.0035
Vacuole Periphery 0.0002 0.0002 0.0005 0.0009 0.0003 0.0003 0.0007 0.002 0.0051 0.0009 0.0019 0.0014 0.0003 0.0002 0.0003 0.0029 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Putative integral membrane protein of unknown function
Localization
Cell Percentages cytoplasm (13%), vacuole (10%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YHR140W

YHR140W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhr140w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available