ORF | |
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Human Ortholog | |
Description | Putative integral membrane protein of unknown function |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 3 | 4 |
Bud | – | 0 | 1 | 0 | 4 | 9 | 20 | 16 | 24 | 12 | 23 | 35 | 4 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 8 | 12 |
Bud Neck | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 1 | 5 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 5 | 4 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 5 | 0 | 5 | 5 | 3 | 9 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | – | 103 | 124 | 99 | 304 | 280 | 482 | 451 | 339 | 384 | 408 | 481 | 362 | 464 | 549 | 269 | 203 | 169 | 1 | 56 | 52 | 132 | 231 | 294 |
Endoplasmic Reticulum | – | 14 | 3 | 1 | 6 | 7 | 7 | 4 | 20 | 14 | 14 | 25 | 5 | 15 | 30 | 12 | 12 | 9 | 0 | 22 | 26 | 7 | 8 | 6 |
Endosome | – | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 2 | 3 | 1 | 5 | 7 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 |
Mitochondria | – | 1 | 7 | 11 | 21 | 20 | 75 | 93 | 74 | 78 | 100 | 132 | 0 | 0 | 0 | 15 | 5 | 0 | 0 | 4 | 4 | 15 | 19 | 26 |
Nucleus | – | 2 | 0 | 1 | 0 | 0 | 4 | 7 | 2 | 4 | 3 | 1 | 1 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | – | 0 | 0 | 2 | 0 | 0 | 1 | 8 | 1 | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 8 |
Vac/Vac Membrane | – | 0 | 4 | 2 | 4 | 5 | 13 | 19 | 13 | 27 | 21 | 21 | 2 | 0 | 4 | 13 | 22 | 11 | 1 | 32 | 51 | 90 | 138 | 116 |
Unique Cell Count | 120 | 132 | 109 | 323 | 304 | 536 | 535 | 423 | 478 | 491 | 601 | 367 | 483 | 583 | 291 | 230 | 179 | 4 | 129 | 143 | 261 | 437 | 500 | |
Labelled Cell Count | 121 | 139 | 116 | 345 | 323 | 606 | 604 | 479 | 534 | 575 | 711 | 381 | 488 | 599 | 316 | 253 | 194 | 4 | 129 | 143 | 261 | 437 | 500 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 5.3 | 4.0 | 3.7 | 4.4 | 3.9 | 3.6 | 4.0 | 3.9 | 4.1 | 4.1 | 4.2 | 5.2 | 5.2 | 5.4 | 4.9 | 5.1 | 5.2 | 5.8 | 5.6 | 5.6 |
Std Deviation (1e-4) | – | 1.0 | 0.7 | 1.1 | 1.5 | 0.9 | 0.9 | 1.3 | 0.9 | 1.0 | 1.1 | 1.2 | 0.8 | 0.8 | 0.9 | 0.9 | 1.1 | 1.1 | 1.3 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.12 | -0.03 | -0.14 | 0.01 | -0.03 | 0.03 | 0.01 | 0.07 | 0.37 | 0.37 | 0.42 | 0.29 | 0.36 | 0.36 | 0.53 | 0.49 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | 0.1 | -0.7 | -1.4 | -2.9 | -3.7 | -3.7 | -3.1 | -3.8 | 2.9 | 1.1 | 0.1 | -0.6 | -1.8 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 1.4 | 1.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 0.6 | 0 | 0 | 0 | 0 | 0.7 | 2.3 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4437 | -0.1555 | -0.3532 | 0.3252 | -0.4295 | -0.2055 | -0.4444 | 0.645 | 0.0533 | -0.042 | -0.7458 | -0.1857 | -2.0964 | -1.285 | -1.7258 | -1.6167 | -2.2786 | -1.9014 |
Actin | 0.0175 | 0.0003 | 0.0055 | 0.0266 | 0.0244 | 0.0017 | 0.0215 | 0.0001 | 0.001 | 0 | 0.0095 | 0.0004 | 0.0061 | 0.0005 | 0.0004 | 0.0047 | 0.0003 | 0.0002 |
Bud | 0.0002 | 0.0004 | 0.0032 | 0.0002 | 0.0002 | 0.0009 | 0.0018 | 0.0001 | 0.0001 | 0 | 0.0025 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0032 | 0 | 0 |
Bud Neck | 0.001 | 0.0002 | 0.0008 | 0.0004 | 0.0024 | 0.002 | 0.0008 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 |
Bud Periphery | 0.0002 | 0.0005 | 0.0032 | 0.0002 | 0.0003 | 0.0037 | 0.003 | 0.0001 | 0.0002 | 0.0001 | 0.0128 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0081 | 0 | 0 |
Bud Site | 0.0038 | 0.002 | 0.0117 | 0.0003 | 0.0003 | 0.0002 | 0.0038 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.001 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0 |
Cytoplasm | 0.0949 | 0.1057 | 0.1042 | 0.0898 | 0.1881 | 0.1483 | 0.0652 | 0.162 | 0.1041 | 0.1165 | 0.1039 | 0.1391 | 0.1701 | 0.2369 | 0.1953 | 0.2351 | 0.2999 | 0.349 |
Cytoplasmic Foci | 0.006 | 0.005 | 0.0042 | 0.014 | 0.004 | 0.009 | 0.0188 | 0.0104 | 0.0075 | 0.0455 | 0.0317 | 0.0112 | 0.0097 | 0.012 | 0.0169 | 0.0194 | 0.0147 | 0.0209 |
Eisosomes | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.011 | 0.003 | 0.0109 | 0.0053 | 0.0043 | 0.0121 | 0.0055 | 0.0073 | 0.0042 | 0.0016 | 0.0071 | 0.0038 | 0.0121 | 0.0089 | 0.0095 | 0.0091 | 0.0028 | 0.0055 |
Endosome | 0.0037 | 0.0025 | 0.0042 | 0.0161 | 0.0043 | 0.0052 | 0.0131 | 0.0234 | 0.0094 | 0.0447 | 0.0556 | 0.0113 | 0.006 | 0.0096 | 0.0059 | 0.0402 | 0.0041 | 0.013 |
Golgi | 0.0007 | 0.0002 | 0.0004 | 0.0023 | 0.0018 | 0.0004 | 0.0017 | 0.0003 | 0.0005 | 0.0005 | 0.0011 | 0.0001 | 0.001 | 0.0002 | 0.0003 | 0.0009 | 0.0002 | 0.0002 |
Lipid Particles | 0.0012 | 0.0006 | 0.0008 | 0.0022 | 0.0018 | 0.0005 | 0.0041 | 0.0007 | 0.0023 | 0.002 | 0.0218 | 0.0005 | 0.0028 | 0.0012 | 0.0033 | 0.0038 | 0.0007 | 0.002 |
Mitochondria | 0.0005 | 0.0004 | 0.0008 | 0.002 | 0.0007 | 0.0004 | 0.0014 | 0.0005 | 0.0023 | 0.0009 | 0.0034 | 0.0003 | 0.0007 | 0.0002 | 0.0005 | 0.0019 | 0.0005 | 0.0002 |
None | 0.8428 | 0.8676 | 0.8303 | 0.7978 | 0.7523 | 0.7992 | 0.833 | 0.7775 | 0.8376 | 0.7688 | 0.7207 | 0.815 | 0.778 | 0.7218 | 0.7552 | 0.6458 | 0.6726 | 0.6001 |
Nuclear Periphery | 0.003 | 0.0015 | 0.0039 | 0.0247 | 0.0048 | 0.0061 | 0.0037 | 0.0019 | 0.0023 | 0.0008 | 0.0106 | 0.0006 | 0.0024 | 0.0024 | 0.0022 | 0.0064 | 0.0008 | 0.0013 |
Nucleolus | 0.002 | 0.0006 | 0.001 | 0.0005 | 0.0006 | 0.0003 | 0.0006 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0001 |
Nucleus | 0.0027 | 0.0036 | 0.0039 | 0.0044 | 0.0018 | 0.0048 | 0.0021 | 0.0012 | 0.0013 | 0.0012 | 0.0023 | 0.0008 | 0.0025 | 0.0017 | 0.0014 | 0.0036 | 0.0008 | 0.0017 |
Peroxisomes | 0.0005 | 0.0001 | 0.0002 | 0.0013 | 0.0005 | 0.0002 | 0.0042 | 0.0001 | 0.0006 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0002 | 0.0027 | 0.0004 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0066 | 0.0038 | 0.0021 | 0.0092 | 0.0058 | 0.0035 | 0.0064 | 0.0003 | 0.0023 | 0.0007 | 0.0008 | 0.0006 | 0.0024 | 0.0006 | 0.0023 | 0.0028 | 0.0007 | 0.0016 |
Vacuole | 0.001 | 0.0013 | 0.0078 | 0.0017 | 0.001 | 0.0011 | 0.0076 | 0.0116 | 0.0176 | 0.0143 | 0.012 | 0.0137 | 0.0025 | 0.0025 | 0.0028 | 0.0089 | 0.0011 | 0.0035 |
Vacuole Periphery | 0.0002 | 0.0002 | 0.0005 | 0.0009 | 0.0003 | 0.0003 | 0.0007 | 0.002 | 0.0051 | 0.0009 | 0.0019 | 0.0014 | 0.0003 | 0.0002 | 0.0003 | 0.0029 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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