Standard name
Human Ortholog
Description Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.21 0.09 0.07 0.06 0 0 0 0 0 0 0 0 0.06 0.06 0 0.06 0 0 0.11 0.05 0 0.06 0.08 0.09
Bud 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.05 0.1 0.08 0.08
Bud Site 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.53 0.53 0.46 0.71 0.47 0.57 0.65 0.69 0.68 0.69 0.75 0.78 0.59 0.7 0.83 0.48 0.53 0.51 0.09 0 0 0 0 0
Endoplasmic Reticulum 0.08 0.1 0.13 0.09 0 0 0 0 0 0 0 0 0.28 0.19 0.08 0 0 0.05 0.16 0.12 0.17 0.2 0.23 0.22
Endosome 0.06 0.07 0.09 0 0.13 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.05 0.13 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0.06 0.1 0.09 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.21 0.27 0.19 0.46 0.36 0.31 0.26 0.37 0.3 0.21 0.2 0 0 0 0.45 0.43 0.38 0.22 0.55 0.61 0.41 0.4 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 40 23 12 4 3 3 3 2 1 4 3 14 15 11 7 4 6 14 29 16 16 28 34
Bud 4 13 8 2 4 4 3 5 6 9 7 12 6 8 1 1 1 0 4 24 18 36 28 34
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 6 44 19 29 30 32
Bud Site 4 13 11 3 3 3 1 2 1 3 4 3 2 2 1 1 0 0 – – – – – –
Cell Periphery 1 3 3 3 1 1 2 4 1 4 1 0 9 11 19 1 2 2 1 1 0 3 7 12
Cytoplasm 41 230 162 143 177 215 258 298 189 274 217 336 132 182 254 60 95 76 11 18 7 5 9 11
Endoplasmic Reticulum 6 45 47 19 12 16 12 11 1 4 2 1 63 50 25 1 4 7 21 66 72 59 82 88
Endosome 5 31 31 5 48 6 2 10 1 10 1 8 4 1 8 12 14 7 16 28 19 4 5 7
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3 2 3 5 11 4 3 9 4
Mitochondria 3 9 4 0 5 17 21 26 27 35 32 44 0 1 0 0 2 3 4 13 5 10 9 12
Nucleus 1 6 4 8 15 34 25 17 12 3 3 6 2 0 1 7 6 8 1 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 4 1 1 2 1 0 0 0 0 0 0 0 1 1 1 1 2 5
Nucleolus 4 1 2 1 3 4 5 2 0 0 2 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 3 3 0 2 0 2 1 2 1 1 1 0 0 0 0 1 2 2 1 0 0 0 0
SpindlePole 5 9 12 2 8 1 1 2 2 4 1 5 6 4 0 0 1 4 2 1 0 0 0 0
Vac/Vac Membrane 4 91 95 38 170 135 123 113 102 119 61 84 5 2 4 56 78 56 29 312 265 120 146 143
Unique Cell Count 78 432 350 201 373 375 397 429 279 398 288 430 222 259 305 125 180 148 133 563 434 294 366 393
Labelled Cell Count 95 495 405 236 452 439 463 495 347 469 337 507 243 278 325 149 210 174 133 563 434 294 366 393


Ambiguous, Bud, Bud Neck, Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 8.4 7.1 7.6 7.2 6.7 5.8 5.8 5.4 5.5 5.2 5.4 8.3 7.2 6.8 10.1 9.9 10.0 11.6 12.3 12.9
Std Deviation (1e-4) 2.2 1.9 1.1 1.3 1.4 1.2 1.4 1.1 1.1 1.2 1.3 1.1 1.1 1.4 1.1 2.0 1.7 1.8 2.3 2.4 2.9
Intensity Change (Log2) – – – 0.11 0.02 -0.07 -0.3 -0.28 -0.39 -0.38 -0.44 -0.39 0.22 0.03 -0.05 0.51 0.49 0.5 0.71 0.8 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 -3.9 -4.2 -4.3 -4.5 -3.7 -4.9 -3.2 -4.5 -0.1 -0.4 -1.7 -0.4 -2.2 -1.1
Bud 0 -1.3 -1.3 -1.7 -1.2 -0.1 0 0.1 0.4 0.3 0.6 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.2 -2.3 -2.3 -3.1 -2.9 -2.5 -2.4 -1.5 -2.6 -1.8 -2.0 -2.7 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 2.8 3.8 0 0 0
Cytoplasm 5.7 0.3 3.0 5.1 6.5 5.4 6.2 7.4 9.2 3.1 5.9 9.8 0.3 1.4 1.0
Endoplasmic Reticulum -1.4 -5.0 -4.4 -5.3 -5.7 -6.1 -6.7 -6.0 -7.6 4.4 2.0 -2.1 -4.0 -4.1 -2.9
Endosome -2.9 1.7 -4.4 -5.5 -4.1 -4.8 -3.8 -4.9 -4.5 -3.4 -4.6 -3.4 0.2 -0.4 -1.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.7 3.1 3.5 4.9 4.7 5.4 5.3 0 0 0 0 0 0
Nucleus 0 2.4 4.8 3.6 2.4 2.5 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.7 -1.1 -3.2 -3.3 -3.1 -2.3 -2.3 -2.7 -2.2 -0.5 -1.4 -3.3 0 0 0
Vacuole -2.2 5.1 2.6 1.2 -0.3 2.5 0.8 -1.7 -2.5 -7.6 -8.8 -9.2 3.6 3.8 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6656 4.9778 4.529 4.2696 4.0092 4.5765 6.243 7.7727 7.5044 7.0892 6.9738 7.8374 5.165 6.8673 6.2382 6.3534 5.1857 6.475
Actin 0.0096 0.0042 0.0101 0.0005 0.0004 0.0015 0.0335 0.0023 0.012 0.0337 0.0037 0.0026 0.0217 0.0014 0.0034 0.0003 0.0233 0.002
Bud 0.007 0.0052 0.0058 0.0012 0.0008 0.0016 0.0028 0.0022 0.0025 0.0018 0.0014 0.0013 0.0027 0.0031 0.0018 0.0006 0.0007 0.0011
Bud Neck 0.018 0.0185 0.0886 0.012 0.0146 0.346 0.0301 0.0131 0.0852 0.0029 0.0033 0.3213 0.0202 0.0269 0.106 0.0019 0.0014 0.3547
Bud Periphery 0.0113 0.0066 0.0063 0.0019 0.0016 0.0041 0.0062 0.0044 0.0054 0.0038 0.0048 0.0036 0.0037 0.0043 0.0034 0.0016 0.001 0.0037
Bud Site 0.1069 0.1724 0.0844 0.0136 0.0075 0.0089 0.1168 0.1272 0.1137 0.009 0.0034 0.0024 0.0856 0.1465 0.0682 0.0017 0.0022 0.0037
Cell Periphery 0.0261 0.0163 0.0057 0.002 0.003 0.0067 0.0297 0.0166 0.018 0.0082 0.0139 0.0098 0.0255 0.0261 0.0126 0.0063 0.0007 0.0106
Cytoplasm 0.1885 0.2356 0.1547 0.0845 0.1317 0.1463 0.1326 0.1894 0.102 0.0535 0.128 0.1253 0.2107 0.1888 0.1003 0.0709 0.1874 0.1211
Cytoplasmic Foci 0.0618 0.0331 0.0548 0.0455 0.0819 0.0289 0.0416 0.027 0.0404 0.0213 0.0342 0.0183 0.029 0.0276 0.0485 0.0272 0.0668 0.0277
Eisosomes 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0003 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0831 0.0635 0.051 0.0258 0.0396 0.0598 0.1289 0.0932 0.073 0.0539 0.0359 0.1041 0.1439 0.0884 0.0707 0.0874 0.0589 0.103
Endosome 0.1788 0.1397 0.2708 0.3794 0.3975 0.1019 0.1427 0.1058 0.2085 0.2482 0.3258 0.1031 0.133 0.1245 0.2792 0.3713 0.4243 0.0878
Golgi 0.0163 0.0126 0.0305 0.0134 0.0211 0.0078 0.0205 0.0096 0.0404 0.0163 0.0122 0.0079 0.0142 0.0146 0.0454 0.0238 0.0797 0.0132
Lipid Particles 0.0693 0.0213 0.0124 0.0086 0.0329 0.0109 0.0649 0.0442 0.0247 0.0084 0.0189 0.0128 0.0646 0.0472 0.0327 0.0166 0.0161 0.0188
Mitochondria 0.0033 0.0044 0.0075 0.0044 0.0036 0.0044 0.0024 0.001 0.0041 0.0648 0.0024 0.0019 0.0029 0.0015 0.0066 0.0025 0.0188 0.002
None 0.016 0.0031 0.0073 0.0097 0.0011 0.0045 0.0096 0.0017 0.0066 0.0004 0.0059 0.002 0.0073 0.0019 0.0071 0.0016 0.0125 0.0024
Nuclear Periphery 0.0123 0.0067 0.0065 0.0064 0.0063 0.0074 0.0168 0.0084 0.0064 0.0058 0.0129 0.0068 0.0115 0.007 0.006 0.0115 0.0155 0.0055
Nucleolus 0.0058 0.0028 0.0017 0.0022 0.0012 0.0039 0.002 0.0017 0.0019 0.0011 0.0021 0.0007 0.0016 0.0026 0.0016 0.0003 0.0004 0.0009
Nucleus 0.0142 0.0079 0.0046 0.0046 0.004 0.0061 0.0066 0.006 0.004 0.0046 0.0065 0.0035 0.0047 0.0056 0.0037 0.0028 0.004 0.0034
Peroxisomes 0.0106 0.0008 0.0045 0.0025 0.0057 0.002 0.0094 0.0006 0.0025 0.0126 0.0016 0.0003 0.0013 0.0005 0.001 0.0005 0.005 0.0007
Punctate Nuclear 0.0068 0.0013 0.001 0.004 0.0018 0.0089 0.0081 0.0009 0.0008 0.0004 0.0163 0.0012 0.002 0.0009 0.0008 0.0005 0.002 0.0008
Vacuole 0.1479 0.237 0.1833 0.3505 0.2304 0.2287 0.1902 0.3373 0.2371 0.433 0.3525 0.2607 0.2074 0.2745 0.1917 0.3472 0.0696 0.228
Vacuole Periphery 0.0061 0.0069 0.0085 0.0272 0.0135 0.0096 0.0043 0.0073 0.0105 0.016 0.0143 0.01 0.0065 0.0062 0.009 0.0234 0.0094 0.0089

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.8686 30.0788 23.0894 37.0569 35.3516 18.5538 25.6934 33.267 30.1119 39.9719
Translational Efficiency 1.2553 1.1909 1.4742 0.9862 1.087 3.308 1.3781 0.9587 1.1277 0.9939

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
Localization
Cell Percentages vacuole (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Chs7

Chs7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Chs7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available