Standard name | |
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Human Ortholog | |
Description | Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 3 | 3 | 2 | 4 | 4 | 4 | 6 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 1 | 5 | 7 | 14 | 25 | 13 | 10 | 18 | 16 | 15 | 0 | 2 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 5 | 5 | 9 | 14 | 7 | 22 | 16 | 11 | 12 | 7 | 3 | 15 | 36 | 60 | 37 | 22 | 35 | 0 | 2 | 2 | 2 | 1 | 1 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 13 | 8 | 13 | 1 | 0 | 0 | 1 | 0 | 1 |
Endosome | 4 | 1 | 0 | 0 | 8 | 1 | 8 | 2 | 2 | 0 | 1 | 0 | 0 | 2 | 1 | 7 | 4 | 11 | 3 | 0 | 1 | 2 | 4 | 3 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 4 | 1 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 5 | 67 | 98 | 146 | 147 | 257 | 328 | 366 | 230 | 276 | 166 | 222 | 13 | 6 | 47 | 18 | 4 | 30 | 2 | 5 | 0 | 1 | 2 | 12 |
Nucleus | 200 | 287 | 191 | 237 | 264 | 347 | 463 | 488 | 229 | 307 | 172 | 193 | 116 | 217 | 232 | 383 | 230 | 344 | 175 | 283 | 177 | 81 | 225 | 146 |
Nuclear Periphery | 4 | 4 | 6 | 4 | 9 | 10 | 21 | 10 | 8 | 13 | 15 | 14 | 0 | 7 | 10 | 7 | 8 | 12 | 2 | 1 | 1 | 0 | 0 | 3 |
Nucleolus | 25 | 12 | 12 | 40 | 71 | 61 | 81 | 90 | 43 | 55 | 27 | 26 | 6 | 17 | 32 | 25 | 22 | 28 | 17 | 9 | 8 | 2 | 10 | 17 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 7 | 19 | 3 | 16 | 11 | 6 | 4 | 4 | 1 | 0 | 3 | 2 | 1 | 3 | 6 | 12 | 2 | 9 | 0 | 4 | 7 |
Vac/Vac Membrane | 3 | 4 | 1 | 3 | 1 | 3 | 6 | 2 | 1 | 1 | 3 | 6 | 0 | 4 | 12 | 21 | 26 | 31 | 10 | 12 | 17 | 10 | 40 | 79 |
Unique Cell Count | 232 | 324 | 222 | 310 | 388 | 464 | 646 | 681 | 334 | 426 | 259 | 291 | 137 | 273 | 335 | 470 | 292 | 453 | 232 | 324 | 227 | 106 | 296 | 285 |
Labelled Cell Count | 251 | 382 | 317 | 452 | 543 | 706 | 972 | 1002 | 544 | 690 | 419 | 483 | 150 | 297 | 399 | 517 | 328 | 516 | 232 | 324 | 227 | 106 | 296 | 285 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 4.9 | 4.1 | 4.1 | 4.1 | 3.8 | 3.8 | 3.8 | 3.5 | 3.4 | 3.5 | 3.1 | 5.9 | 6.2 | 5.2 | 8.7 | 9.2 | 9.5 | 5.5 | 5.5 | 5.7 |
Std Deviation (1e-4) | 1.3 | 1.2 | 0.6 | 1.2 | 0.9 | 0.6 | 0.9 | 0.9 | 1.0 | 0.8 | 1.2 | 1.0 | 1.9 | 2.0 | 1.5 | 2.2 | 2.3 | 2.6 | 1.6 | 1.3 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.02 | -0.1 | -0.11 | -0.1 | -0.24 | -0.26 | -0.22 | -0.38 | 0.52 | 0.6 | 0.35 | 1.08 | 1.17 | 1.22 | 0.42 | 0.43 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.8 | 4.3 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.018 | 3.4904 | 3.9373 | 2.8495 | 3.2573 | 3.0626 | 3.4041 | 4.1734 | 4.1569 | 3.1254 | 3.4916 | 3.4133 | 2.7365 | 4.0381 | 3.6283 | 3.718 | 2.8779 | 3.248 |
Actin | 0.0422 | 0 | 0 | 0 | 0.0018 | 0.0049 | 0.0669 | 0 | 0.004 | 0 | 0.0173 | 0.0019 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 |
Bud | 0.0011 | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0.0011 | 0 | 0.0016 | 0 | 0.003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 |
Bud Neck | 0.0034 | 0.0001 | 0.0002 | 0.0001 | 0.002 | 0.0013 | 0.0036 | 0.0001 | 0.0013 | 0 | 0.0004 | 0.0006 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0015 |
Bud Periphery | 0.0026 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0015 | 0.0015 | 0.0001 | 0.0016 | 0 | 0.0032 | 0.0001 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0.0001 |
Bud Site | 0.0066 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0025 | 0.0001 | 0.0066 | 0 | 0.0068 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0007 | 0 | 0.0001 | 0 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0069 | 0.0001 | 0.0001 | 0.0002 | 0.0014 | 0 | 0.0145 | 0.003 | 0.0016 | 0.0001 | 0.0035 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0016 | 0.0001 |
Cytoplasmic Foci | 0.0117 | 0 | 0 | 0 | 0.0059 | 0.0001 | 0.0155 | 0 | 0.015 | 0 | 0.0123 | 0.0007 | 0 | 0 | 0.0006 | 0 | 0.0163 | 0 |
Eisosomes | 0.001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0058 | 0 | 0 | 0 | 0.0018 | 0.0011 | 0.0136 | 0.0004 | 0.0007 | 0 | 0.0009 | 0.001 | 0.0002 | 0 | 0.0003 | 0 | 0.0004 | 0 |
Endosome | 0.0261 | 0 | 0 | 0.0001 | 0.0331 | 0.0015 | 0.0318 | 0.0001 | 0.0115 | 0 | 0.0183 | 0.0087 | 0.0003 | 0.0001 | 0.0023 | 0 | 0.0086 | 0 |
Golgi | 0.0126 | 0 | 0 | 0 | 0.0039 | 0.0015 | 0.0122 | 0 | 0.0051 | 0 | 0.022 | 0.0057 | 0 | 0 | 0.0006 | 0 | 0.0003 | 0 |
Lipid Particles | 0.0343 | 0.0001 | 0 | 0 | 0.0139 | 0.0005 | 0.0141 | 0 | 0.0047 | 0 | 0.0215 | 0.0007 | 0 | 0 | 0.0011 | 0 | 0.0031 | 0 |
Mitochondria | 0.0566 | 0.0136 | 0.0004 | 0.0003 | 0.0059 | 0.0033 | 0.0108 | 0.0002 | 0.0101 | 0.0001 | 0.0166 | 0.0023 | 0.0008 | 0.0002 | 0.0071 | 0.0002 | 0.0003 | 0.0001 |
None | 0.0071 | 0 | 0 | 0 | 0.001 | 0.0002 | 0.0075 | 0.0087 | 0.001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0008 | 0 |
Nuclear Periphery | 0.0403 | 0.0008 | 0.0125 | 0.0032 | 0.0418 | 0.0127 | 0.0493 | 0.0049 | 0.0166 | 0.0005 | 0.0011 | 0.0068 | 0.0054 | 0.0042 | 0.007 | 0.0033 | 0.0474 | 0.0012 |
Nucleolus | 0.0151 | 0.0303 | 0.0896 | 0.0125 | 0.1028 | 0.0505 | 0.0091 | 0.0206 | 0.0605 | 0.0083 | 0.0138 | 0.0294 | 0.0174 | 0.0144 | 0.0573 | 0.045 | 0.0272 | 0.0503 |
Nucleus | 0.6845 | 0.9426 | 0.8936 | 0.9829 | 0.7604 | 0.9153 | 0.7031 | 0.9535 | 0.8196 | 0.9906 | 0.8493 | 0.9323 | 0.9727 | 0.9786 | 0.9063 | 0.9504 | 0.8608 | 0.9416 |
Peroxisomes | 0.0124 | 0.0001 | 0 | 0 | 0.0011 | 0 | 0.0195 | 0 | 0.0074 | 0 | 0.0059 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0 |
Punctate Nuclear | 0.0209 | 0.0115 | 0.0025 | 0.0002 | 0.0134 | 0.0014 | 0.0108 | 0.0066 | 0.0225 | 0.0004 | 0.0009 | 0.008 | 0.0012 | 0.0007 | 0.0102 | 0.0007 | 0.0315 | 0.004 |
Vacuole | 0.004 | 0.0002 | 0.0003 | 0.0002 | 0.0042 | 0.0011 | 0.0075 | 0.0012 | 0.0045 | 0 | 0.0017 | 0.0004 | 0.0005 | 0.0003 | 0.0028 | 0 | 0.0003 | 0.0006 |
Vacuole Periphery | 0.004 | 0.0002 | 0.0003 | 0.0002 | 0.0039 | 0.0017 | 0.0041 | 0.0003 | 0.0038 | 0 | 0.001 | 0.0004 | 0.0003 | 0.0003 | 0.0008 | 0 | 0.0008 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.826 | 14.4288 | 5.4673 | 12.2024 | 32.7264 | 35.6958 | 11.1745 | 7.3325 | 7.9284 | 36.2966 |
Translational Efficiency | 0.9506 | 0.6883 | 0.5977 | 0.5569 | 0.7554 | 1.0836 | 0.7816 | 0.5809 | 0.7397 | 0.6742 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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