Standard name | |
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Human Ortholog | |
Description | Putative sterol transfer protein; localizes to puncta in the cortical ER; probable role in retrograde transport of sterols from the plasma membrane to the ER; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; contains GRAM, StART-like (VASt) and two PH-like domains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 8 | 51 | 3 | 39 | 11 | 19 | 21 |
Bud | 0 | 1 | 2 | 5 | 3 | 14 | 14 | 4 | 17 | 20 | 10 | 29 | 0 | 0 | 5 | 1 | 4 | 6 | 3 | 0 | 0 | 0 | 8 | 1 |
Bud Neck | 5 | 0 | 7 | 4 | 3 | 1 | 8 | 6 | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 4 | 2 | 11 | 2 | 9 | 5 | 8 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 5 | 0 | 4 | 3 | 5 | 3 | 5 | 7 | 9 | 12 | 4 | 4 | 8 | 5 | 7 | 15 | 6 | 12 | 22 | 4 | 31 | 6 | 13 | 19 |
Cytoplasm | 239 | 39 | 259 | 167 | 271 | 270 | 349 | 335 | 263 | 256 | 120 | 133 | 120 | 136 | 143 | 109 | 130 | 127 | 91 | 29 | 89 | 30 | 70 | 50 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 2 | 1 | 12 | 8 | 6 | 13 | 1 | 3 | 0 | 1 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 0 | 4 | 1 | 4 | 1 | 4 | 2 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 10 | 9 | 22 | 23 | 80 | 112 | 101 | 146 | 193 | 122 | 182 | 0 | 4 | 2 | 10 | 5 | 11 | 55 | 10 | 82 | 17 | 13 | 20 |
Nucleus | 0 | 0 | 1 | 1 | 1 | 1 | 4 | 5 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 2 | 1 | 4 | 3 | 4 | 4 | 9 | 6 | 1 | 6 | 0 | 0 | 0 | 3 | 6 | 5 | 3 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 5 | 4 | 3 | 7 | 1 | 5 | 1 | 3 | 1 |
Unique Cell Count | 249 | 47 | 273 | 177 | 290 | 311 | 415 | 403 | 349 | 390 | 212 | 278 | 121 | 142 | 147 | 143 | 154 | 157 | 275 | 58 | 276 | 79 | 153 | 130 |
Labelled Cell Count | 256 | 51 | 286 | 204 | 312 | 374 | 498 | 462 | 451 | 495 | 263 | 362 | 129 | 150 | 160 | 168 | 178 | 187 | 275 | 58 | 276 | 79 | 153 | 130 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 3.3 | 3.2 | 3.2 | 3.5 | 3.0 | 3.3 | 3.3 | 2.8 | 2.7 | 2.8 | 2.6 | 4.4 | 4.8 | 4.4 | 7.2 | 6.4 | 6.5 | 4.1 | 4.6 | 5.1 |
Std Deviation (1e-4) | 0.9 | 0.6 | 0.6 | 1.4 | 1.3 | 0.8 | 1.7 | 1.5 | 1.5 | 1.2 | 1.3 | 1.3 | 2.2 | 2.5 | 2.5 | 4.6 | 3.0 | 3.5 | 1.3 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.14 | -0.09 | 0.03 | 0.04 | -0.19 | -0.22 | -0.19 | -0.27 | 0.47 | 0.6 | 0.47 | 1.17 | 1.0 | 1.04 | 0.37 | 0.51 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0054 | 0.0205 | 0.0204 | 0.0068 | 0.0573 | 0.0162 | 0.0573 | 0.0055 | 0.0156 | 0.0207 | 0.0066 | 0.0358 | 0.0001 | 0.0608 | 0.0729 | 0.1017 | 0.0229 | 0.0194 |
Bud | 0.0001 | 0.0005 | 0.0004 | 0.0007 | 0.0004 | 0.0001 | 0.004 | 0.0013 | 0.0025 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0022 | 0.0012 | 0.0004 | 0.0001 |
Bud Neck | 0.0013 | 0.0042 | 0.0062 | 0.0062 | 0.0186 | 0.0356 | 0.0044 | 0.0039 | 0.0113 | 0.0141 | 0.0383 | 0.0201 | 0.0001 | 0.0018 | 0.0163 | 0.0026 | 0.0049 | 0.0286 |
Bud Periphery | 0.0006 | 0.0017 | 0.0018 | 0.0035 | 0.0018 | 0.0007 | 0.0112 | 0.0026 | 0.0108 | 0.0013 | 0.0006 | 0.0027 | 0 | 0.0007 | 0.0052 | 0.0035 | 0.0009 | 0.0005 |
Bud Site | 0.0026 | 0.0538 | 0.0194 | 0.0153 | 0.0026 | 0.0024 | 0.021 | 0.0851 | 0.0455 | 0.0054 | 0.0011 | 0.0009 | 0 | 0.02 | 0.0551 | 0.003 | 0.0018 | 0.0003 |
Cell Periphery | 0.0227 | 0.0212 | 0.0117 | 0.0212 | 0.0126 | 0.0081 | 0.0055 | 0.0176 | 0.01 | 0.0101 | 0.0043 | 0.0072 | 0.0032 | 0.0039 | 0.0095 | 0.0058 | 0.002 | 0.004 |
Cytoplasm | 0.4152 | 0.5109 | 0.4789 | 0.5598 | 0.4705 | 0.5644 | 0.1422 | 0.3163 | 0.3209 | 0.3319 | 0.3607 | 0.3641 | 0.0784 | 0.0966 | 0.2706 | 0.1764 | 0.2131 | 0.3503 |
Cytoplasmic Foci | 0.0098 | 0.0107 | 0.0151 | 0.0456 | 0.0117 | 0.0106 | 0.0313 | 0.0102 | 0.0264 | 0.0373 | 0.0569 | 0.0333 | 0.003 | 0.0269 | 0.0189 | 0.0223 | 0.0387 | 0.0074 |
Eisosomes | 0.0039 | 0.0021 | 0.001 | 0.0004 | 0.0015 | 0.0007 | 0.0022 | 0.0011 | 0.0012 | 0.0007 | 0.0007 | 0.0009 | 0.0006 | 0.0066 | 0.0026 | 0.0025 | 0.0026 | 0.0008 |
Endoplasmic Reticulum | 0.0034 | 0.0039 | 0.0055 | 0.0034 | 0.0017 | 0.0037 | 0.0171 | 0.0019 | 0.0035 | 0.013 | 0.0013 | 0.0014 | 0.0012 | 0.0039 | 0.0048 | 0.0057 | 0.0024 | 0.0023 |
Endosome | 0.0006 | 0.0013 | 0.0011 | 0.0004 | 0.0004 | 0.0012 | 0.0243 | 0.0004 | 0.0172 | 0.0301 | 0.0005 | 0.0102 | 0.0001 | 0.0051 | 0.0222 | 0.0091 | 0.0063 | 0.0006 |
Golgi | 0.0006 | 0.0019 | 0.0014 | 0.0005 | 0.0007 | 0.0006 | 0.0154 | 0.0003 | 0.0098 | 0.0129 | 0.0008 | 0.0082 | 0 | 0.0076 | 0.0219 | 0.0074 | 0.0016 | 0.0004 |
Lipid Particles | 0.0008 | 0.002 | 0.002 | 0.0003 | 0.0003 | 0.0024 | 0.0385 | 0.0005 | 0.0026 | 0.0177 | 0.0002 | 0.0053 | 0.0009 | 0.0021 | 0.0031 | 0.0032 | 0.0025 | 0.0004 |
Mitochondria | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0116 | 0.0002 | 0.0094 | 0.0099 | 0.0002 | 0.003 | 0 | 0.0005 | 0.0239 | 0.0048 | 0.0008 | 0.0001 |
None | 0.5304 | 0.3614 | 0.4314 | 0.3318 | 0.4185 | 0.351 | 0.5516 | 0.5505 | 0.5074 | 0.4329 | 0.5238 | 0.4875 | 0.9116 | 0.7392 | 0.457 | 0.6382 | 0.6881 | 0.5836 |
Nuclear Periphery | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0047 | 0.0001 | 0.0005 | 0.0041 | 0.0001 | 0.0024 | 0 | 0.0017 | 0.0007 | 0.0012 | 0.0003 | 0.0001 |
Nucleolus | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0029 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0006 | 0.0004 | 0.0003 | 0.0001 |
Nucleus | 0.0004 | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0077 | 0.0005 | 0.0006 | 0.001 | 0.0003 | 0.0004 | 0 | 0.0015 | 0.0008 | 0.0012 | 0.0004 | 0.0002 |
Peroxisomes | 0.0003 | 0.0008 | 0.0014 | 0.0024 | 0.0003 | 0.0005 | 0.0331 | 0.0005 | 0.0018 | 0.0495 | 0.0027 | 0.0078 | 0 | 0.0015 | 0.0055 | 0.0077 | 0.0023 | 0.0002 |
Punctate Nuclear | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0076 | 0.0004 | 0.0005 | 0.0008 | 0.0003 | 0.008 | 0 | 0.0179 | 0.0008 | 0.0012 | 0.001 | 0.0002 |
Vacuole | 0.0008 | 0.0011 | 0.0008 | 0.0004 | 0.0004 | 0.0006 | 0.0052 | 0.0008 | 0.0017 | 0.0041 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0041 | 0.0008 | 0.0064 | 0.0005 |
Vacuole Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0.0005 | 0.0017 | 0 | 0.0002 | 0 | 0.0001 | 0.0013 | 0.0002 | 0.0004 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.3102 | 10.7011 | 10.9185 | 19.0626 | 14.1626 | 12.8614 | 14.5392 | 18.1655 | 18.7739 | 21.8453 |
Translational Efficiency | 0.3541 | 0.3522 | 0.4324 | 0.289 | 0.3369 | 0.3959 | 0.3759 | 0.2401 | 0.2532 | 0.2679 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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