Standard name | |
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Human Ortholog | |
Description | Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 3 | 3 | 1 | 2 | 3 |
Bud | 2 | 1 | 0 | 1 | 0 | 2 | 7 | 6 | 4 | 9 | 10 | 4 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 1 | 0 | 3 | 5 |
Bud Neck | 0 | 0 | 7 | 12 | 12 | 11 | 2 | 14 | 7 | 7 | 3 | 9 | 6 | 2 | 4 | – | – | 0 | 1 | 2 | 1 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 0 | 0 | 0 | – | – | 1 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 3 | 3 | 1 | 0 | 0 | 1 | 1 | – | – | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
Cytoplasm | 245 | 217 | 210 | 282 | 434 | 418 | 458 | 559 | 483 | 544 | 345 | 346 | 204 | 345 | 318 | – | – | 34 | 213 | 183 | 145 | 109 | 215 | 254 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | 12 | 2 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | 2 | 4 | 4 | 2 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 2 | 0 | 1 | 1 | 1 |
Mitochondria | 31 | 5 | 0 | 4 | 4 | 46 | 131 | 114 | 225 | 142 | 224 | 101 | 0 | 0 | 0 | – | – | 5 | 22 | 20 | 25 | 5 | 8 | 9 |
Nucleus | 1 | 1 | 0 | 0 | 7 | 5 | 3 | 6 | 8 | 5 | 11 | 11 | 2 | 8 | 1 | – | – | 82 | 2 | 7 | 2 | 1 | 6 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 1 | 6 | 4 | 6 | 11 | 7 | 13 | 5 | 10 | 0 | 0 | 2 | – | – | 0 | 2 | 2 | 2 | 1 | 4 | 4 |
Vac/Vac Membrane | 1 | 7 | 1 | 0 | 6 | 2 | 9 | 3 | 5 | 5 | 6 | 9 | 0 | 7 | 7 | – | – | 53 | 11 | 14 | 5 | 8 | 8 | 10 |
Unique Cell Count | 266 | 230 | 214 | 289 | 452 | 446 | 527 | 638 | 610 | 638 | 475 | 419 | 206 | 359 | 326 | 168 | 275 | 249 | 206 | 134 | 264 | 307 | ||
Labelled Cell Count | 282 | 232 | 222 | 304 | 474 | 490 | 616 | 717 | 743 | 732 | 610 | 491 | 212 | 364 | 333 | 190 | 275 | 249 | 206 | 134 | 264 | 307 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 4.1 | 4.5 | 4.0 | 4.4 | 3.5 | 3.0 | 3.6 | 2.9 | 3.3 | 2.8 | 3.3 | 4.5 | 4.8 | 4.7 | – | – | 6.0 | 4.6 | 4.7 | 5.2 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.2 | 1.5 | 2.2 | 1.3 | 1.2 | 0.7 | 1.0 | 1.0 | 1.0 | 1.0 | 1.2 | 1.2 | 1.3 | – | – | 1.6 | 1.8 | 1.5 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.05 | -0.38 | -0.58 | -0.33 | -0.65 | -0.47 | -0.71 | -0.46 | -0.01 | 0.07 | 0.04 | – | – | 0.4 | 0.01 | 0.06 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 8.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.3374 | -0.7705 | -1.1155 | -1.041 | -1.6822 | -0.8205 | -0.849 | -0.1344 | -0.4042 | -0.7555 | -0.7885 | -0.1895 | -1.2013 | -0.5094 | -0.9023 | -0.9296 | -0.8457 | -1.066 |
Actin | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0.0083 | 0.0003 | 0.0009 | 0.0001 | 0.0005 | 0.0007 |
Bud | 0.0008 | 0.0023 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0013 | 0.0054 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0077 | 0.004 | 0 | 0.0014 | 0.0003 |
Bud Neck | 0.0022 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0012 | 0.0004 | 0.0031 | 0.0004 | 0.0004 | 0.001 | 0.0005 | 0.0012 | 0.0002 | 0.0002 | 0.0003 | 0.0007 |
Bud Periphery | 0.0004 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0018 | 0.0011 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0016 | 0.0009 | 0.0001 | 0.0008 | 0.0002 |
Bud Site | 0.0009 | 0.0036 | 0.0002 | 0 | 0 | 0.0001 | 0.0014 | 0.0034 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0076 | 0.001 | 0 | 0.0002 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 |
Cytoplasm | 0.0221 | 0.0274 | 0.03 | 0.061 | 0.0559 | 0.0398 | 0.0457 | 0.0552 | 0.0461 | 0.058 | 0.0196 | 0.068 | 0.0694 | 0.0836 | 0.0931 | 0.0596 | 0.1429 | 0.1247 |
Cytoplasmic Foci | 0.0081 | 0.0026 | 0.0022 | 0.0017 | 0.0109 | 0.0066 | 0.0035 | 0.0025 | 0.0038 | 0.0062 | 0.0075 | 0.0042 | 0.0054 | 0.0079 | 0.0071 | 0.0048 | 0.0116 | 0.0071 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0005 | 0.0006 | 0.0004 | 0.0003 | 0.0012 | 0.0003 | 0.0003 | 0.0007 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0008 | 0.0014 | 0.0008 | 0.0006 | 0.0019 | 0.0008 |
Endosome | 0.0005 | 0.0013 | 0.0003 | 0.0005 | 0.001 | 0.0007 | 0.0003 | 0.0005 | 0.0004 | 0.0009 | 0.0002 | 0.0007 | 0.0028 | 0.0024 | 0.0024 | 0.0008 | 0.0031 | 0.0043 |
Golgi | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Lipid Particles | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0009 | 0.0002 | 0.0005 | 0.0006 | 0.002 | 0.0003 |
Mitochondria | 0.0051 | 0.0003 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0008 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0007 | 0.0006 | 0.0004 | 0.001 | 0.0007 | 0.0014 | 0.0006 |
None | 0.9275 | 0.9289 | 0.9173 | 0.9087 | 0.8671 | 0.8874 | 0.8741 | 0.8762 | 0.8808 | 0.7988 | 0.8589 | 0.8007 | 0.8844 | 0.8465 | 0.8475 | 0.9229 | 0.7732 | 0.7933 |
Nuclear Periphery | 0.0011 | 0.004 | 0.0039 | 0.0017 | 0.0074 | 0.0015 | 0.0008 | 0.0022 | 0.0012 | 0.0016 | 0.0011 | 0.0024 | 0.0015 | 0.0014 | 0.0019 | 0.0011 | 0.004 | 0.002 |
Nucleolus | 0.0008 | 0.0015 | 0.0009 | 0.0009 | 0.0008 | 0.0011 | 0.0012 | 0.0015 | 0.0013 | 0.001 | 0.0023 | 0.002 | 0.002 | 0.001 | 0.0012 | 0.0006 | 0.0015 | 0.0008 |
Nucleus | 0.0242 | 0.0178 | 0.0314 | 0.0196 | 0.0459 | 0.0448 | 0.0594 | 0.031 | 0.0432 | 0.0677 | 0.0585 | 0.0837 | 0.0148 | 0.0243 | 0.0291 | 0.003 | 0.0423 | 0.0562 |
Peroxisomes | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0036 | 0.001 | 0.0002 | 0.0006 | 0.0003 | 0.0006 | 0.0003 |
Punctate Nuclear | 0.0039 | 0.0077 | 0.0118 | 0.0044 | 0.0057 | 0.0151 | 0.0068 | 0.0183 | 0.0158 | 0.0637 | 0.0497 | 0.0305 | 0.0044 | 0.0097 | 0.006 | 0.0037 | 0.0086 | 0.0064 |
Vacuole | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0016 | 0.0018 | 0.001 | 0.0004 | 0.0025 | 0.0009 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.3144 | 9.642 | 6.4393 | 5.2327 | 4.1953 | 3.234 | 4.3871 | 6.2509 | 4.2933 | 5.6197 |
Translational Efficiency | 1.8618 | 0.6028 | 1.0506 | 1.374 | 1.3669 | 3.3388 | 1.4144 | 0.8994 | 1.694 | 1.2577 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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