Standard name
Human Ortholog
Description Tripartite DNA replication factor; single-stranded DNA-dependent ATPase, ATP-dependent nuclease, helicase; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair/processing of meiotic DNA double strand breaks; component of telomeric chromatin with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress; human homolog DNA2 complements yeast dna2 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.53 0.43 0.58 0.62 0.51 0.5 0.62 0.59 0.49 0.55 0.49 0.5 0.63 0.55 0.63 0.56 0.52 0.57 0.38 0.37 0.44 0.72 0.91 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.4 0.35 0.54 0.32 0.48 0.44 0.5 0.66 0.61 0.66 0.66 0 0 0.15 0 0 0 0 0 0.08 0 0 0
Nucleus 0.37 0.19 0.15 0.18 0.21 0.24 0.15 0.14 0.11 0.13 0.1 0.1 0.27 0.26 0.09 0.24 0.21 0.17 0.36 0.36 0.24 0.11 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.17 0 0 0 0 0 0 0 0 0 0 0.11 0.16 0.22 0.23 0.28 0.29 0.1 0.14 0.14 0.09 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Bud 0 0 1 1 4 7 8 13 4 6 4 4 0 1 6 0 0 0 3 1 1 1 1 3
Bud Neck 0 0 0 0 1 0 3 1 0 1 0 1 0 0 0 1 0 0 0 0 0 1 1 0
Bud Site 0 0 0 0 0 3 6 6 3 2 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 1 1 0 0 1 0 1 1 2 5 2 3 0 0 0 0 0 0
Cytoplasm 10 47 80 35 106 93 166 207 94 148 90 127 172 145 193 426 277 359 28 43 66 117 349 298
Endoplasmic Reticulum 0 0 0 1 0 0 0 1 1 1 0 0 0 2 0 42 26 47 0 0 0 1 0 1
Endosome 0 0 0 0 6 0 0 0 0 0 2 2 2 7 1 22 34 43 1 2 2 0 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 2 44 48 30 67 90 117 176 128 166 121 166 6 3 45 8 13 11 1 2 11 1 5 8
Nucleus 7 21 21 10 43 44 39 48 22 35 18 26 75 70 29 181 112 110 27 42 36 17 5 0
Nuclear Periphery 0 0 0 0 0 1 2 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 2 0 2 0 0 5 1 1 1 0 0 0 0 3 3 4 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 5 1 3 6 3 2 2 3 1 0 1 7 5 4 3 4 4 4 5 4
Vac/Vac Membrane 3 19 5 0 8 6 3 5 1 4 4 3 31 43 66 172 152 180 7 16 20 15 7 2
Unique Cell Count 19 110 139 56 207 186 268 353 193 271 183 252 275 265 306 760 537 631 76 119 150 163 383 328
Labelled Cell Count 22 132 159 77 242 246 348 470 259 366 246 333 288 272 344 868 625 761 76 119 150 163 383 328


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.4 3.5 3.5 3.4 3.3 2.9 3.1 2.7 2.9 2.9 2.5 4.6 4.8 4.0 6.0 6.1 6.4 4.7 4.5 5.2
Std Deviation (1e-4) 0.6 0.5 1.0 2.0 0.9 1.1 1.0 1.4 1.0 1.6 1.2 0.8 0.7 0.8 0.9 1.3 1.3 1.5 0.9 0.8 1.1
Intensity Change (Log2) – – – 0.02 -0.01 -0.07 -0.23 -0.14 -0.35 -0.27 -0.27 -0.47 0.41 0.46 0.23 0.79 0.83 0.9 0.45 0.4 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.2 6.1 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4164 1.1862 0.8814 0.6918 1.2919 0.8956 1.4485 1.6505 1.5637 1.47 1.327 1.5574 0.9674 1.3425 1.3263 1.3611 1.0345 1.202
Actin 0.0316 0.0008 0.0131 0.0002 0.0189 0.0004 0.0276 0.0002 0.0113 0.0051 0.001 0.0026 0.0022 0.0001 0.0252 0 0.0004 0.0005
Bud 0.0031 0.0008 0.0005 0.0001 0.0021 0.0003 0.0023 0.0052 0.0061 0.0005 0.0002 0.0013 0.0024 0.0001 0.0008 0 0.0001 0.0007
Bud Neck 0.0067 0.0017 0.0063 0.0012 0.0015 0.0103 0.0019 0.0007 0.0011 0.0021 0.0011 0.005 0.0008 0.0002 0.0028 0.0003 0.0005 0.0042
Bud Periphery 0.0063 0.001 0.0011 0.0001 0.0044 0.0004 0.0027 0.0018 0.0065 0.0007 0.0002 0.0016 0.0026 0 0.0013 0 0.0002 0.0009
Bud Site 0.0059 0.0138 0.0039 0.0004 0.0043 0.0005 0.0038 0.0075 0.0076 0.0008 0.0033 0.0005 0.0023 0.0006 0.0028 0.0002 0.0002 0.0026
Cell Periphery 0.0014 0.0005 0.0009 0.0001 0.0005 0.0003 0.0004 0.0002 0.0003 0.0001 0.0001 0.0004 0.0004 0.0001 0.0004 0 0.0001 0.0001
Cytoplasm 0.3133 0.2529 0.0944 0.0779 0.1038 0.2933 0.3085 0.2069 0.0418 0.0678 0.0561 0.3071 0.2206 0.1071 0.0507 0.0423 0.0354 0.1945
Cytoplasmic Foci 0.0322 0.0107 0.0163 0.0076 0.0132 0.005 0.043 0.0079 0.0064 0.0052 0.0352 0.0151 0.019 0.0012 0.0061 0.0126 0.0012 0.0065
Eisosomes 0.0005 0.0001 0.0006 0 0.0002 0 0.0003 0 0.0002 0 0 0 0.0001 0.0001 0.0007 0 0 0.0001
Endoplasmic Reticulum 0.0209 0.0077 0.0038 0.0024 0.0051 0.0039 0.0134 0.0026 0.0013 0.0016 0.002 0.0026 0.0094 0.0022 0.0088 0.0008 0.0036 0.0034
Endosome 0.0202 0.0031 0.0061 0.0081 0.02 0.0077 0.0525 0.003 0.0127 0.0153 0.0239 0.0124 0.0062 0.0013 0.0166 0.0081 0.0081 0.0028
Golgi 0.0044 0.0003 0.0027 0.0001 0.0073 0.0007 0.0171 0.0002 0.0066 0.0124 0.0071 0.006 0.0016 0 0.0063 0.0001 0.0001 0.0002
Lipid Particles 0.0104 0.0007 0.0078 0.0003 0.016 0.0101 0.0329 0.0002 0.0093 0.0063 0.0058 0.0009 0.0182 0.0002 0.0014 0.0005 0.0002 0.0004
Mitochondria 0.0137 0.0008 0.0125 0.0007 0.041 0.001 0.0216 0.0008 0.0105 0.0087 0.0073 0.0035 0.0029 0.0004 0.0075 0.0003 0.0009 0.0008
None 0.3286 0.2762 0.1047 0.0619 0.1078 0.1084 0.2505 0.1906 0.0664 0.0463 0.0647 0.1215 0.4776 0.2583 0.0885 0.0133 0.0393 0.3294
Nuclear Periphery 0.0261 0.02 0.0136 0.0127 0.0202 0.0081 0.0255 0.0084 0.0222 0.011 0.0208 0.0088 0.0135 0.0091 0.0308 0.0072 0.0116 0.0084
Nucleolus 0.0064 0.0039 0.0128 0.006 0.0086 0.0061 0.0021 0.0035 0.0055 0.0048 0.0044 0.0033 0.0029 0.0038 0.0053 0.004 0.0074 0.0096
Nucleus 0.1049 0.3466 0.6283 0.6802 0.5798 0.531 0.11 0.5397 0.7136 0.7258 0.7406 0.4592 0.1781 0.5997 0.6995 0.8366 0.8827 0.4014
Peroxisomes 0.0063 0.0006 0.0123 0.0001 0.0105 0.0007 0.0407 0.0005 0.0082 0.002 0.0029 0.0055 0.0094 0 0.0011 0.0001 0.0001 0.0003
Punctate Nuclear 0.031 0.0493 0.0528 0.1368 0.0258 0.0072 0.0316 0.0143 0.0569 0.0813 0.0118 0.0348 0.0165 0.0133 0.0374 0.0653 0.0041 0.0304
Vacuole 0.0219 0.0076 0.0045 0.0027 0.0064 0.0039 0.0087 0.0051 0.0043 0.0015 0.0098 0.0059 0.0116 0.0019 0.0046 0.0078 0.003 0.0023
Vacuole Periphery 0.004 0.0009 0.001 0.0004 0.0026 0.0004 0.0028 0.0006 0.0013 0.0007 0.0015 0.0021 0.0016 0.0002 0.0016 0.0005 0.001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.7619 9.7148 8.3302 11.1411 6.5754 8.4478 11.1869 11.123 11.766 11.504
Translational Efficiency 0.7979 0.5724 0.6476 0.521 0.7304 0.7555 0.5357 0.4818 0.4951 0.5244

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Tripartite DNA replication factor; single-stranded DNA-dependent ATPase, ATP-dependent nuclease, helicase; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair/processing of meiotic DNA double strand breaks; component of telomeric chromatin with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress; human homolog DNA2 complements yeast dna2 mutant
Localization
Cell Percentages nucleus (38%), cytoplasm (10%), mixed (41%)
Cell Cycle Regulation Yes
nucleus - S/G2 (0.00055)
Subcompartmental Group N/A

Dna2

Dna2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dna2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available