Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.06 0.07 0.06 0.07 0.09 0 0.06 0.12 0.14 0.2 0.08 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.16 0.14 0.36 0.29 0.68 0.41 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.9 0.96 0.87 0.88 0.74 0.8 0.79 0.68 0.73 0.72 0.88 0.85 0.78 0.73 0.71 0.77 0.87 0.89 0.73 0.73 0.76 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.08 0 0.05 0.05 0.08 0.05 0 0 0 0.05 0.08 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.12 0 0 0.05 0.07 0 0 0.06 0 0 0 0 0 0.14 0.12 0.07 0 0.05 0.16 0.12 0.1 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 5 4 0 2 0 1 0 0 0 0 2 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 1 2 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 2 4 3 9 10 13 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 0 14 12 16 24 14 26 6 20 8 21 49 23 27 15 0 0 2 0 1 7
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 1 1 8 4 3 0 0 0 0 0 1
Endosome 0 2 0 0 1 2 8 0 0 0 1 0 0 0 7 7 6 1 2 1 1 3 4
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 4 2 1 1 1 0 0 0 0
Mitochondria 15 1 0 5 3 38 55 69 80 179 131 0 0 0 12 11 5 0 1 0 2 2 5
Nucleus 174 207 22 151 173 174 324 154 191 191 232 56 129 190 207 168 129 178 204 52 37 145 136
Nuclear Periphery 0 0 0 0 0 0 0 1 1 1 3 0 0 1 0 1 0 0 0 0 0 0 0
Nucleolus 4 3 0 1 4 19 17 9 15 21 15 0 1 2 13 18 12 6 4 0 0 7 7
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 2 8 10 5 6 3 7 0 2 3 3 2 4 2 1 0 2 8 2
Vac/Vac Membrane 9 27 1 7 9 17 12 6 17 6 14 1 0 4 39 29 11 8 11 11 5 18 36
Unique Cell Count 188 230 23 173 197 234 405 194 279 263 323 64 152 245 284 238 167 205 230 73 51 191 209
Labelled Cell Count 205 247 23 182 204 276 455 270 351 417 439 65 155 252 316 269 186 205 230 73 51 191 209


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.8 6.1 6.4 6.5 5.2 5.4 4.4 4.9 4.0 4.5 6.9 6.7 6.2 9.0 8.7 8.6 5.2 6.2 6.6
Std Deviation (1e-4) 0.6 0.9 1.0 1.7 1.1 1.0 1.3 1.2 1.1 0.6 1.2 1.2 1.4 1.5 1.8 1.9 2.3 1.1 1.2 1.4
Intensity Change (Log2) 0.07 0.09 -0.23 -0.18 -0.46 -0.33 -0.62 -0.44 0.18 0.13 0.03 0.55 0.51 0.5 -0.23 0.02 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.2 -1.1 -2.3 -1.9 -1.9 -2.7 -2.4 -2.5 -1.1 -1.4 -2.0 -2.4 -2.6 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0139 0.6641 0.5753 -0.2649 -0.0402 0.5269 1.369 2.1158 1.8289 1.4486 1.4732 1.5718 0.5178 1.0257 0.968 0.8694 0.7005 0.7315
Actin 0.0158 0 0.0115 0.0003 0.0075 0.0014 0.014 0.0001 0.004 0 0.0015 0.0022 0.0093 0 0.0002 0 0.0003 0
Bud 0.0002 0 0.0005 0.0001 0.0029 0.0001 0.0002 0.0005 0.0002 0 0.0003 0 0.0002 0 0.0001 0 0 0
Bud Neck 0.0008 0.0001 0.0016 0.0002 0.0013 0.0035 0.0014 0.0003 0.0006 0.0002 0.0011 0.0011 0.0022 0 0.0002 0 0.0049 0.0014
Bud Periphery 0.0003 0 0.0007 0.0004 0.0073 0.0002 0.0007 0.0013 0.0004 0.0002 0.0007 0.0001 0.0004 0 0.0001 0 0 0
Bud Site 0.0025 0 0.0126 0.0006 0.0027 0.0004 0.0011 0.0086 0.0005 0.0001 0.002 0.0001 0.0009 0 0.0001 0 0.0002 0.0001
Cell Periphery 0.0003 0 0.0002 0.0003 0.0006 0 0.0002 0.0003 0.0001 0.0001 0.0009 0 0.0002 0 0 0 0 0
Cytoplasm 0.0111 0.0001 0.0013 0.0009 0.0079 0.0021 0.0045 0.0005 0.0012 0 0.0042 0.001 0.001 0.0001 0.001 0.0001 0.0019 0.0009
Cytoplasmic Foci 0.0155 0 0.0139 0.003 0.0038 0.0046 0.0051 0.0001 0.0038 0 0.0009 0.0011 0.0004 0 0 0 0.0135 0.0008
Eisosomes 0.0004 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0021 0 0.0002 0.0025 0.0063 0.0003 0.0012 0.0002 0.0013 0 0.0056 0.0003 0.0014 0 0.0003 0 0.0003 0
Endosome 0.0211 0 0.0099 0.0196 0.0361 0.0024 0.0097 0.0004 0.0112 0 0.0074 0.0017 0.0033 0 0.0003 0 0.0019 0.0002
Golgi 0.0057 0 0.0066 0.0028 0.0079 0.0016 0.0035 0 0.002 0 0.0017 0.0007 0.0009 0 0 0 0.0002 0
Lipid Particles 0.0195 0 0.0183 0.0483 0.0246 0.0044 0.0166 0.0001 0.0081 0 0.0027 0.0014 0.0009 0 0 0 0.0025 0
Mitochondria 0.0099 0.0001 0.0142 0.0079 0.0606 0.0012 0.0111 0.0002 0.0031 0.0026 0.0022 0.0009 0.001 0.0001 0.0005 0.0001 0.0002 0.0001
None 0.0466 0 0.001 0.0007 0.0068 0.0003 0.0022 0.0004 0.0017 0 0.0005 0.0011 0.001 0 0.0002 0 0.0046 0.0001
Nuclear Periphery 0.017 0.0014 0.0087 0.011 0.055 0.0062 0.0077 0.0026 0.0062 0.0008 0.0132 0.0029 0.0071 0.0042 0.0068 0.0026 0.0061 0.0028
Nucleolus 0.017 0.0107 0.0105 0.0142 0.0431 0.0261 0.0194 0.013 0.0088 0.0294 0.0253 0.0225 0.0154 0.006 0.0087 0.0039 0.0316 0.0199
Nucleus 0.7575 0.9859 0.8724 0.8703 0.6881 0.9204 0.881 0.9692 0.9395 0.9653 0.9208 0.9508 0.9485 0.9768 0.9798 0.9929 0.9051 0.9524
Peroxisomes 0.0202 0 0.0108 0.0007 0.0031 0.0068 0.0046 0 0.0012 0 0.0002 0.0018 0.0002 0 0 0 0.0004 0
Punctate Nuclear 0.0324 0.0013 0.0023 0.0025 0.0221 0.0165 0.0118 0.0012 0.003 0.0004 0.0054 0.01 0.003 0.0125 0.0008 0.0002 0.0258 0.0208
Vacuole 0.0027 0 0.0019 0.009 0.0065 0.0009 0.0025 0.001 0.0021 0.0003 0.0026 0.0002 0.0018 0 0.0005 0 0.0003 0.0002
Vacuole Periphery 0.0012 0 0.0007 0.0048 0.0057 0.0004 0.0012 0.0001 0.0009 0.0006 0.0009 0.0002 0.0006 0 0.0002 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6259 7.8658 3.8912 8.5903 11.7887 2.9314 9.9414 8.9464 10.4607 9.9486
Translational Efficiency 1.3052 1.0353 1.4241 0.7109 0.7015 2.6868 0.9228 0.9792 0.7017 0.7435

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cdc23

Cdc23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available