Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 18 | 11 | 4 | 12 | 3 | 6 | 1 | 2 | 6 | 2 | 5 | 4 | 1 | 0 | 5 | 2 | 8 | 39 | 47 | 16 | 6 | 16 | 20 |
Bud | 8 | 16 | 3 | 8 | 6 | 8 | 15 | 6 | 6 | 4 | 16 | 20 | 4 | 7 | 11 | 3 | 1 | 5 | 30 | 21 | 13 | 19 | 56 | 45 |
Bud Neck | 2 | 8 | 17 | 6 | 14 | 7 | 13 | 4 | 3 | 1 | 5 | 7 | 1 | 0 | 0 | 7 | 8 | 4 | 21 | 32 | 18 | 11 | 21 | 8 |
Bud Site | 8 | 9 | 5 | 7 | 5 | 7 | 7 | 1 | 2 | 4 | 3 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 10 | 4 | 6 | 3 | 7 | 1 | 0 | 1 | 4 |
Cytoplasm | 303 | 392 | 131 | 70 | 159 | 135 | 159 | 104 | 79 | 60 | 91 | 87 | 126 | 140 | 166 | 265 | 153 | 210 | 217 | 266 | 55 | 12 | 57 | 58 |
Endoplasmic Reticulum | 4 | 7 | 2 | 0 | 1 | 2 | 1 | 0 | 3 | 4 | 1 | 0 | 1 | 1 | 1 | 25 | 8 | 16 | 4 | 4 | 2 | 0 | 2 | 1 |
Endosome | 4 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 13 | 10 | 5 | 8 | 10 | 14 | 10 | 2 | 5 | 3 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 3 | 0 | 1 | 1 | 2 | 0 |
Mitochondria | 23 | 22 | 1 | 9 | 3 | 31 | 40 | 16 | 58 | 65 | 115 | 78 | 16 | 5 | 7 | 7 | 4 | 7 | 52 | 82 | 46 | 9 | 18 | 20 |
Nucleus | 2 | 4 | 0 | 3 | 2 | 1 | 5 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 7 | 0 | 1 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 6 | 8 | 4 | 4 | 2 | 2 | 2 | 1 | 3 | 5 | 4 |
SpindlePole | 17 | 9 | 14 | 18 | 22 | 15 | 26 | 9 | 2 | 6 | 4 | 16 | 0 | 4 | 4 | 26 | 11 | 10 | 12 | 17 | 8 | 4 | 20 | 15 |
Vac/Vac Membrane | 5 | 1 | 2 | 1 | 0 | 3 | 3 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 10 | 3 | 5 | 11 | 15 | 4 | 1 | 7 | 3 |
Unique Cell Count | 360 | 454 | 162 | 105 | 195 | 194 | 228 | 121 | 124 | 116 | 188 | 170 | 144 | 162 | 197 | 325 | 181 | 254 | 420 | 526 | 186 | 77 | 219 | 191 |
Labelled Cell Count | 393 | 490 | 191 | 130 | 227 | 214 | 278 | 143 | 157 | 153 | 239 | 222 | 154 | 171 | 215 | 378 | 207 | 285 | 420 | 526 | 186 | 77 | 219 | 191 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 4.0 | 4.9 | 4.4 | 5.0 | 3.8 | 4.0 | 4.2 | 3.4 | 3.2 | 3.1 | 3.6 | 4.1 | 4.3 | 4.5 | 6.6 | 7.3 | 6.1 | 4.4 | 4.4 | 4.6 |
Std Deviation (1e-4) | 0.6 | 0.5 | 1.4 | 1.4 | 1.6 | 1.1 | 1.2 | 1.3 | 1.2 | 0.6 | 0.5 | 0.8 | 1.1 | 0.9 | 1.4 | 2.1 | 11.9 | 1.9 | 1.6 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.01 | -0.37 | -0.29 | -0.22 | -0.52 | -0.61 | -0.67 | -0.46 | -0.24 | -0.2 | -0.13 | 0.43 | 0.58 | 0.32 | -0.16 | -0.17 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0312 | -0.2261 | -0.1362 | -0.8995 | -0.0229 | -0.2338 | 1.2405 | 0.4584 | 0.7694 | 0.388 | 0.3334 | 0.6207 | -0.1899 | -0.3182 | -0.0446 | 0.0947 | -0.13 | -0.1718 |
Actin | 0.0758 | 0.0158 | 0.0495 | 0.0042 | 0.0702 | 0.0283 | 0.0915 | 0.0291 | 0.0248 | 0.0098 | 0.0234 | 0.0496 | 0.0631 | 0.0096 | 0.0371 | 0.0077 | 0.0826 | 0.0296 |
Bud | 0.004 | 0.0039 | 0.025 | 0.0003 | 0.0153 | 0.0028 | 0.0109 | 0.009 | 0.0241 | 0.0344 | 0.0048 | 0.0034 | 0.0115 | 0.0097 | 0.0194 | 0.0169 | 0.0141 | 0.0008 |
Bud Neck | 0.0273 | 0.01 | 0.0156 | 0.0016 | 0.0047 | 0.1982 | 0.0243 | 0.0119 | 0.0041 | 0.0064 | 0.0877 | 0.2831 | 0.0309 | 0.0057 | 0.0024 | 0.0013 | 0.0045 | 0.171 |
Bud Periphery | 0.0139 | 0.0063 | 0.055 | 0.0011 | 0.0483 | 0.0052 | 0.029 | 0.0155 | 0.0575 | 0.0627 | 0.0064 | 0.0103 | 0.028 | 0.0132 | 0.0488 | 0.061 | 0.0403 | 0.0014 |
Bud Site | 0.0617 | 0.1196 | 0.0429 | 0.0009 | 0.0085 | 0.0051 | 0.0473 | 0.1547 | 0.0481 | 0.0091 | 0.0077 | 0.0044 | 0.0567 | 0.1354 | 0.0236 | 0.0052 | 0.0058 | 0.0024 |
Cell Periphery | 0.0018 | 0.003 | 0.0018 | 0.0021 | 0.002 | 0.001 | 0.0017 | 0.0033 | 0.0023 | 0.0008 | 0.0004 | 0.0015 | 0.0027 | 0.0014 | 0.0013 | 0.0012 | 0.0011 | 0.0005 |
Cytoplasm | 0.1411 | 0.1965 | 0.1897 | 0.1901 | 0.3642 | 0.1959 | 0.128 | 0.2353 | 0.2187 | 0.266 | 0.2588 | 0.142 | 0.1574 | 0.1832 | 0.1918 | 0.1405 | 0.2134 | 0.2785 |
Cytoplasmic Foci | 0.1672 | 0.0862 | 0.0755 | 0.037 | 0.0957 | 0.1364 | 0.1341 | 0.0994 | 0.0597 | 0.07 | 0.0985 | 0.1714 | 0.1505 | 0.0609 | 0.0623 | 0.0388 | 0.1209 | 0.1186 |
Eisosomes | 0.0012 | 0.0009 | 0.0007 | 0.001 | 0.0034 | 0.0004 | 0.0014 | 0.0009 | 0.0006 | 0.0001 | 0.0002 | 0.0005 | 0.0011 | 0.0005 | 0.0007 | 0.0002 | 0.0006 | 0.0003 |
Endoplasmic Reticulum | 0.0081 | 0.0036 | 0.0026 | 0.008 | 0.007 | 0.0023 | 0.0078 | 0.0032 | 0.0046 | 0.0017 | 0.0041 | 0.0025 | 0.0063 | 0.0029 | 0.0032 | 0.0029 | 0.0043 | 0.0027 |
Endosome | 0.0444 | 0.0148 | 0.0252 | 0.0283 | 0.0696 | 0.0162 | 0.0641 | 0.0245 | 0.0287 | 0.031 | 0.0386 | 0.0192 | 0.0424 | 0.0118 | 0.0229 | 0.0594 | 0.0454 | 0.0242 |
Golgi | 0.0186 | 0.0036 | 0.0106 | 0.0014 | 0.018 | 0.0102 | 0.0245 | 0.005 | 0.0074 | 0.0097 | 0.0263 | 0.0101 | 0.0181 | 0.0033 | 0.0086 | 0.0133 | 0.0137 | 0.0096 |
Lipid Particles | 0.0359 | 0.0058 | 0.0114 | 0.0081 | 0.0064 | 0.006 | 0.0214 | 0.0037 | 0.0033 | 0.0006 | 0.0137 | 0.0081 | 0.012 | 0.003 | 0.0039 | 0.0013 | 0.0193 | 0.0044 |
Mitochondria | 0.0103 | 0.003 | 0.0085 | 0.0016 | 0.0092 | 0.0112 | 0.0237 | 0.0049 | 0.013 | 0.0157 | 0.0126 | 0.0093 | 0.0134 | 0.0027 | 0.0161 | 0.0387 | 0.0202 | 0.0066 |
None | 0.3212 | 0.5014 | 0.4515 | 0.7015 | 0.2274 | 0.3478 | 0.3245 | 0.3763 | 0.4789 | 0.4472 | 0.3642 | 0.216 | 0.3363 | 0.5364 | 0.5252 | 0.5691 | 0.3535 | 0.3 |
Nuclear Periphery | 0.0127 | 0.0008 | 0.0007 | 0.0018 | 0.0087 | 0.005 | 0.0123 | 0.0007 | 0.0016 | 0.0009 | 0.0027 | 0.0008 | 0.0047 | 0.0009 | 0.0029 | 0.0017 | 0.0044 | 0.0023 |
Nucleolus | 0.0022 | 0.0008 | 0.0012 | 0.0003 | 0.001 | 0.0003 | 0.0012 | 0.001 | 0.001 | 0.0005 | 0.0007 | 0.0007 | 0.0053 | 0.0007 | 0.0006 | 0.0007 | 0.0007 | 0.0004 |
Nucleus | 0.0032 | 0.0014 | 0.0017 | 0.0007 | 0.0036 | 0.0007 | 0.0028 | 0.0017 | 0.0024 | 0.002 | 0.003 | 0.0011 | 0.0043 | 0.0024 | 0.0028 | 0.0023 | 0.0014 | 0.0012 |
Peroxisomes | 0.0308 | 0.0144 | 0.022 | 0.001 | 0.0167 | 0.0222 | 0.032 | 0.0125 | 0.0093 | 0.0147 | 0.0361 | 0.0593 | 0.0395 | 0.0096 | 0.0181 | 0.0017 | 0.0291 | 0.0389 |
Punctate Nuclear | 0.0094 | 0.0024 | 0.0038 | 0.0008 | 0.0035 | 0.0033 | 0.0078 | 0.0027 | 0.0025 | 0.0014 | 0.0059 | 0.005 | 0.0104 | 0.0039 | 0.0036 | 0.0175 | 0.0192 | 0.0044 |
Vacuole | 0.0076 | 0.005 | 0.0043 | 0.0071 | 0.0117 | 0.001 | 0.0071 | 0.0041 | 0.0055 | 0.0115 | 0.0033 | 0.0013 | 0.0045 | 0.0025 | 0.0035 | 0.0143 | 0.0039 | 0.0016 |
Vacuole Periphery | 0.0016 | 0.0007 | 0.0009 | 0.0009 | 0.0048 | 0.0007 | 0.0027 | 0.0006 | 0.0021 | 0.0038 | 0.001 | 0.0004 | 0.0011 | 0.0003 | 0.0009 | 0.0045 | 0.0017 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.6153 | 10.0395 | 8.6913 | 7.7775 | 10.314 | 4.2091 | 10.6796 | 9.8485 | 7.9721 | 9.6527 |
Translational Efficiency | 0.7906 | 0.5971 | 0.4534 | 0.4647 | 0.3519 | 0.8159 | 0.4836 | 0.3246 | 0.5804 | 0.4864 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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