Standard name
Human Ortholog
Description One of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.05 0 0 0.06 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.5 0.8 0.93 0.87 0.77 0.56 0.51 – 0.36 0.26 0.21 0.26 0.89 0.9 0.89 0.39 0.35 0.35 0.18 0.49 0.22 0.25 0.29 0.23
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0.21 0.11 0.16 0 0 0 0.06 0.07 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 0.15 0 0 0.06 0.51 0.25 – 0.66 0.67 0.81 0.76 0 0 0 0.1 0.15 0.12 0.58 0.33 0.53 0.52 0.42 0.45
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.1 0.06 0.14 – 0.07 0.11 0.07 0.1 0 0 0 0.16 0.17 0.07 0.06 0 0.06 0.05 0.05 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0.07 – 0 0 0 0 0 0 0 0.13 0.14 0.22 0.07 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 2 2 0 0 – 0 2 1 0 3 0 0 2 7 5 0 0 2 3 13 16
Bud 0 1 0 4 4 7 2 – 21 16 16 28 3 1 3 8 5 6 0 0 0 1 8 11
Bud Neck 0 0 2 8 11 7 18 – 3 14 4 4 3 0 0 2 1 3 1 0 1 0 5 14
Bud Site 0 0 0 0 0 1 1 – 4 2 9 10 0 0 0 0 1 2 – – – – – –
Cell Periphery 1 0 0 5 0 0 4 – 2 4 0 3 1 3 2 5 3 6 0 0 0 0 2 5
Cytoplasm 10 57 25 239 224 111 211 – 159 114 78 124 217 375 459 61 37 41 10 54 18 42 117 107
Endoplasmic Reticulum 1 2 1 0 0 3 2 – 2 1 4 0 2 0 0 11 9 5 0 0 0 1 3 11
Endosome 0 0 0 1 8 0 14 – 3 5 7 7 5 24 26 32 12 19 2 2 3 10 26 26
Golgi 0 1 0 1 0 0 0 – 0 0 0 0 1 1 0 1 3 0 0 0 0 1 1 3
Mitochondria 12 11 0 11 18 100 105 – 292 296 298 368 9 5 15 16 16 14 35 37 43 88 166 212
Nucleus 0 0 0 1 1 7 12 – 14 10 4 6 0 1 0 2 4 3 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 1 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 1 0 0 – 1 0 2 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 1 2 0 1 – 0 4 2 5 2 6 18 9 3 9 0 0 0 1 3 5
SpindlePole 0 1 0 15 29 12 56 – 29 50 26 50 5 8 7 25 18 8 3 2 4 9 21 23
Vac/Vac Membrane 0 0 0 3 13 2 28 – 12 10 7 15 4 6 19 21 15 26 4 5 3 3 10 13
Unique Cell Count 20 71 27 274 290 197 412 444 441 366 485 243 418 517 156 107 117 61 112 82 171 401 469
Labelled Cell Count 24 76 28 291 313 250 454 543 528 458 622 255 430 549 196 134 147 61 112 82 171 401 469


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.8 4.7 4.4 5.0 3.4 4.1 – 3.7 3.8 3.5 3.7 4.7 4.7 4.8 5.6 6.0 6.0 4.3 4.3 4.5
Std Deviation (1e-4) 0.3 0.6 0.5 1.1 1.3 1.1 0.9 – 1.5 1.1 0.6 0.6 1.5 1.3 1.4 1.3 1.1 1.3 1.9 1.4 1.7
Intensity Change (Log2) – – – -0.08 0.1 -0.47 -0.19 – -0.34 -0.31 -0.41 -0.34 0.01 0.02 0.04 0.26 0.36 0.35 -0.12 -0.12 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7356 -0.7229 -0.8459 -0.5024 -0.0015 -0.7421 -1.3239 -0.2521 -0.7068 -0.7489 -1.3588 -0.5406 1.0243 0.5247 0.2975 0.2495 0.2238 0.4896
Actin 0.0169 0.0039 0.0048 0.01 0.0353 0.0081 0.0166 0.0012 0.0026 0.0039 0.0014 0.0023 0.045 0.0038 0.0077 0.0002 0.0002 0.0008
Bud 0.0081 0.0138 0.0184 0.0077 0.0847 0.0145 0.0008 0.0008 0.0021 0.001 0.0011 0.0003 0.0004 0.0006 0.0005 0.0002 0.0001 0.0006
Bud Neck 0.0078 0.0008 0.0044 0.0058 0.0032 0.0042 0.0024 0.0013 0.0046 0.0022 0.0003 0.0193 0.0028 0.0002 0.0006 0.0004 0.0002 0.0143
Bud Periphery 0.0052 0.0062 0.0083 0.0061 0.0272 0.0081 0.0022 0.0014 0.0027 0.0038 0.0026 0.0008 0.0007 0.0008 0.0009 0.0005 0.0001 0.0019
Bud Site 0.0179 0.0404 0.0251 0.0388 0.0181 0.0014 0.0061 0.0094 0.0087 0.0008 0.0005 0.0003 0.0047 0.0038 0.0021 0.0002 0.0001 0.0013
Cell Periphery 0.0021 0.0017 0.0022 0.0008 0.0022 0.0011 0.0009 0.0014 0.0008 0.0009 0.0002 0.0004 0.0007 0.0005 0.0008 0.0006 0.0001 0.0011
Cytoplasm 0.0919 0.1118 0.0874 0.0636 0.0809 0.1409 0.1327 0.2361 0.1693 0.2774 0.0969 0.2828 0.0965 0.1474 0.1465 0.2332 0.1898 0.2496
Cytoplasmic Foci 0.086 0.1172 0.1048 0.0798 0.0882 0.1187 0.1142 0.0924 0.1083 0.0632 0.0581 0.08 0.1039 0.0511 0.0539 0.0231 0.0421 0.0742
Eisosomes 0.0012 0.0007 0.0025 0.0006 0.0007 0.0004 0.0009 0.0008 0.0009 0.0006 0.0004 0.0002 0.0012 0.0002 0.0006 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0085 0.0049 0.0026 0.0011 0.005 0.0027 0.0071 0.0051 0.0081 0.0038 0.0038 0.0035 0.006 0.0027 0.0038 0.0042 0.0012 0.0035
Endosome 0.069 0.0468 0.0329 0.0322 0.0294 0.0365 0.0959 0.0485 0.0729 0.0221 0.0801 0.0636 0.0366 0.0066 0.0273 0.0147 0.0294 0.0313
Golgi 0.0082 0.0064 0.0065 0.0082 0.0098 0.0066 0.009 0.0076 0.0078 0.0029 0.0112 0.0068 0.0114 0.0033 0.0049 0.0005 0.0041 0.0031
Lipid Particles 0.0181 0.0097 0.0135 0.0097 0.03 0.008 0.0245 0.0099 0.0068 0.0016 0.012 0.0059 0.033 0.0017 0.0068 0.0013 0.0694 0.0044
Mitochondria 0.018 0.0175 0.025 0.0115 0.0367 0.0311 0.0134 0.0266 0.0363 0.0494 0.0775 0.0276 0.0078 0.0017 0.008 0.0026 0.0367 0.01
None 0.5664 0.5883 0.6104 0.6834 0.437 0.5835 0.5416 0.5294 0.5451 0.5412 0.633 0.4897 0.5765 0.7665 0.7121 0.6767 0.6053 0.5928
Nuclear Periphery 0.0185 0.0019 0.0013 0.0012 0.0153 0.0022 0.0028 0.001 0.002 0.0013 0.0015 0.0007 0.0082 0.0004 0.0008 0.0007 0.0006 0.0009
Nucleolus 0.0063 0.0019 0.0039 0.003 0.0078 0.0016 0.0008 0.0023 0.0013 0.0006 0.0006 0.0005 0.0021 0.0002 0.0003 0.0007 0.0003 0.0003
Nucleus 0.0123 0.002 0.0052 0.0028 0.0143 0.003 0.0015 0.002 0.0023 0.0016 0.0012 0.0011 0.0038 0.0006 0.0006 0.0022 0.0009 0.0007
Peroxisomes 0.0095 0.0107 0.0275 0.021 0.0119 0.006 0.0078 0.0075 0.0055 0.0124 0.0056 0.0026 0.0237 0.0056 0.015 0.0005 0.0119 0.0035
Punctate Nuclear 0.0158 0.0041 0.0061 0.0083 0.0541 0.0146 0.0062 0.0013 0.0022 0.0009 0.0012 0.0018 0.0287 0.0006 0.0013 0.0009 0.0006 0.0018
Vacuole 0.0097 0.0077 0.0058 0.0036 0.0059 0.0052 0.0098 0.0125 0.008 0.0062 0.0079 0.0084 0.005 0.0013 0.0047 0.0353 0.0054 0.003
Vacuole Periphery 0.0028 0.0016 0.0014 0.0009 0.0023 0.0015 0.003 0.0015 0.0018 0.0022 0.0028 0.0014 0.0011 0.0002 0.0009 0.0013 0.0013 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.777 5.1643 1.2774 5.9686 4.9934 5.7738 9.5729 14.0969 10.7311 8.9069
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description One of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2
Localization
Cell Percentages mitochondrion (31%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pth1

Pth1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pth1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available