Standard name
Human Ortholog
Description Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.08 – 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.97 0.95 0.88 0.79 – 0.77 0.66 0.75 0.67 0.98 0.99 0.99 0.98 0.99 0.99 0.96 0.94 0.93 0.89 0.89 0.91
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.15 – 0.15 0.21 0.14 0.21 0 0 0 0 0.06 0.05 0 0 0 0 0.06 0.06
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0.05 0.12 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 6 5 – 6 16 10 16 0 0 0 0 1 0 0 0 1 0 1 0
Bud 0 0 0 1 1 6 1 – 0 2 0 1 1 0 1 0 0 0 0 0 0 2 3 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 3 7 8 32 – 11 23 7 14 4 2 10 3 3 2 0 0 0 0 0 0
Cytoplasm 315 118 196 277 311 251 337 – 207 244 138 187 327 445 531 203 226 204 296 105 180 130 312 322
Endoplasmic Reticulum 2 1 0 4 13 23 63 – 40 78 26 58 8 6 3 7 13 11 3 2 4 5 19 20
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 3 7 6 – 13 45 17 24 0 0 0 0 0 0 0 0 0 2 1 1
Mitochondria 0 0 0 0 0 2 0 – 2 1 1 6 0 0 0 1 0 2 0 0 0 1 4 0
Nucleus 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 – 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 2 1 3 – 1 2 0 1 0 0 2 0 0 2 0 1 2 0 1 1
Unique Cell Count 317 119 197 285 328 286 426 268 372 184 281 334 451 536 207 228 206 309 112 195 147 351 355
Labelled Cell Count 317 119 197 288 337 304 447 281 413 200 308 340 453 547 215 243 221 309 112 195 147 351 355


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 124.4 125.8 97.0 90.1 81.9 53.1 60.6 – 50.8 50.2 47.7 46.2 144.1 120.0 144.6 148.4 145.1 149.5 128.5 127.5 138.2
Std Deviation (1e-4) 24.0 23.6 18.9 21.4 17.9 17.0 15.2 – 13.1 12.1 13.5 11.9 24.5 25.3 25.3 44.5 46.9 43.9 25.4 32.4 35.9
Intensity Change (Log2) – – – -0.11 -0.24 -0.87 -0.68 – -0.93 -0.95 -1.02 -1.07 0.57 0.31 0.58 0.61 0.58 0.62 0.41 0.39 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 3.0 0 3.4 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.9 – 0 3.6 0 3.2 0 0 0 0 0 0
Cytoplasm -1.8 -2.9 -4.8 -6.7 – -7.0 -9.2 -7.3 -8.9 -1.5 -0.9 -0.6 -1.3 -0.5 -0.5
Endoplasmic Reticulum 0 0 4.1 5.7 – 5.7 6.9 5.5 6.8 0 0 0 0 3.4 3.3
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 2.7 4.8 4.0 3.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 77.7995 102.3133 91.122 87.283 81.9955 91.2113 104.3063 130.6514 123.1096 115.8477 116.2892 124.4623 93.5307 123.7187 115.5283 110.8974 114.8998 97.0828
Actin 0.0044 0.0003 0.0005 0.0001 0.019 0.0031 0.0012 0.0007 0.0005 0.0001 0.0005 0.0017 0.0026 0.0003 0.0003 0.0016 0.0001 0.0128
Bud 0.0013 0.0005 0.0008 0.0004 0.0008 0.0037 0.0011 0.0004 0.0007 0.0005 0.0007 0.0011 0.0019 0.0007 0.0009 0.0014 0.0007 0.0004
Bud Neck 0.0006 0.0007 0.0007 0.0004 0.0011 0.0026 0.0005 0.0004 0.0005 0.0004 0.0008 0.0011 0.0005 0.0002 0.0004 0.0004 0.0004 0.0007
Bud Periphery 0.0018 0.0003 0.0005 0.0001 0.0014 0.004 0.0013 0.0003 0.0004 0.0002 0.0002 0.0009 0.0019 0.0002 0.0003 0.0003 0.0001 0.0002
Bud Site 0.0005 0.0003 0.0003 0 0.0005 0.0006 0.0005 0.0003 0.0003 0.0001 0.0002 0.0002 0.0008 0.0005 0.0004 0.0004 0 0.0011
Cell Periphery 0.0151 0.0074 0.0072 0.0004 0.0011 0.0065 0.0211 0.0152 0.0095 0.0034 0.0014 0.0021 0.0061 0.0011 0.0027 0.0003 0.0004 0.0002
Cytoplasm 0.7198 0.9145 0.8719 0.896 0.7372 0.6631 0.7438 0.911 0.8753 0.6679 0.7982 0.7976 0.7988 0.97 0.91 0.8992 0.7785 0.8093
Cytoplasmic Foci 0.0031 0.0019 0.0025 0.0014 0.0046 0.0094 0.0036 0.0015 0.004 0.0015 0.0099 0.0072 0.0053 0.0014 0.0024 0.004 0.0187 0.0342
Eisosomes 0.0002 0 0 0 0.0002 0.0001 0.0008 0.0004 0 0 0.0004 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0636 0.0266 0.0218 0.0009 0.0898 0.0441 0.0454 0.0165 0.0153 0.0151 0.0122 0.0369 0.0288 0.0021 0.0025 0.0006 0.0014 0.0066
Endosome 0.0147 0.0057 0.0099 0.0049 0.0211 0.0474 0.0097 0.0028 0.0057 0.0079 0.0274 0.0382 0.0131 0.0025 0.0073 0.011 0.0612 0.0799
Golgi 0.0035 0.002 0.0015 0.0001 0.0045 0.028 0.0025 0.0013 0.0008 0.0002 0.0247 0.0117 0.002 0.0003 0.0012 0.0008 0.006 0.0267
Lipid Particles 0.0013 0.0007 0.0007 0 0.0049 0.0022 0.0019 0.0017 0.0011 0.0004 0.0032 0.0004 0.001 0 0.0004 0.0001 0.0003 0.0058
Mitochondria 0.008 0.0007 0.0009 0.0001 0.0044 0.0262 0.0139 0.0034 0.0008 0.0001 0.0094 0.0174 0.0061 0.0004 0.0084 0.0005 0.0007 0.0032
None 0.0015 0.0002 0.0003 0.0001 0.0005 0.0012 0.0019 0.0014 0.0008 0.0002 0.003 0.0013 0.0022 0.0002 0.0004 0.0002 0.0002 0.0006
Nuclear Periphery 0.0172 0.0028 0.0042 0.0021 0.0064 0.0183 0.0225 0.0042 0.0093 0.0059 0.0041 0.0048 0.0176 0.0008 0.0025 0.0016 0.0034 0.0025
Nucleolus 0.0032 0.0001 0.0002 0.0001 0.0001 0.0005 0.0033 0.0007 0.0004 0.0002 0.0108 0.0005 0.0011 0 0.0003 0.0001 0.0001 0.0001
Nucleus 0.0067 0.0011 0.0019 0.0017 0.0022 0.0038 0.0053 0.0016 0.0022 0.0025 0.0024 0.0032 0.0066 0.0008 0.0019 0.0017 0.0019 0.0007
Peroxisomes 0.0003 0 0 0 0.0023 0.0001 0.0002 0.0001 0.0001 0 0.0007 0.0001 0.0001 0 0 0.0001 0.0001 0.0044
Punctate Nuclear 0.0003 0 0 0 0.0004 0.0002 0.0041 0.0026 0.0002 0 0.0001 0.0001 0.0001 0 0 0 0 0.0019
Vacuole 0.104 0.0294 0.0623 0.0793 0.0748 0.1003 0.0889 0.0295 0.0609 0.2222 0.0677 0.049 0.0779 0.0161 0.0458 0.0584 0.0769 0.0058
Vacuole Periphery 0.0289 0.0048 0.0121 0.0119 0.0226 0.0345 0.0264 0.0041 0.0112 0.0711 0.0219 0.0244 0.0253 0.0022 0.0117 0.0172 0.0488 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 790.2478 768.434 673.379 776.4329 829.755 846.5254 747.518 717.3605 709.0911 681.3787
Translational Efficiency 2.9092 2.9564 3.1234 2.5659 2.936 2.8726 2.4064 2.6801 2.5386 2.6782

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Egd2

Egd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Egd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available