Standard name
Human Ortholog
Description Non-ATPase base subunit of the 19S RP of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the regulatory particle (RP); binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.79 0.72 0.69 0.61 0.86 0.84 0.83 0.81 0.78 0.77 0.74 0.66 0.85 0.72 0.76 0.99 0.93 0.94 0.72 0.56 0.67 0.66 0.65 0.52
Endoplasmic Reticulum 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0.14 0.38 0.34 0.34 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.21 0.12 0.08 0.14 0.19
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.34 0.46 0.35 0.1 0.07 0.12 0.09 0.05 0 0 0.06 0.1 0.35 0.29 0 0.09 0.06 0.06 0.1 0.05 0.13 0.06 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 0 0 1 0 2 3 4 0 0 0 0 0 0 1 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 5 1 4 2 4 7 0 0 1 2 1 0 0 0 0 0 0 0
Cytoplasm 67 109 126 71 150 215 196 185 74 105 148 171 53 104 141 141 43 44 83 92 132 32 87 82
Endoplasmic Reticulum 4 0 2 5 3 15 6 1 2 0 2 3 4 1 4 5 2 3 1 1 3 1 3 3
Endosome 0 0 1 0 0 1 0 0 0 0 0 1 0 1 2 1 0 0 6 5 7 0 4 5
Golgi 1 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Mitochondria 3 3 0 0 6 12 13 33 36 47 69 116 0 0 0 4 1 0 1 2 1 1 1 3
Nucleus 1 5 6 5 1 3 2 3 1 4 1 2 0 3 7 0 1 1 6 34 24 4 18 29
Nuclear Periphery 0 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 3 3 4 0 2 4
Nucleolus 0 0 0 1 0 0 1 0 0 1 0 2 0 1 0 0 0 0 1 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 1
Vac/Vac Membrane 14 51 85 41 17 18 28 20 5 3 3 16 6 51 53 1 4 3 6 16 10 6 8 20
Unique Cell Count 85 151 183 117 174 257 237 229 95 137 201 258 62 145 185 142 46 47 116 164 199 50 135 159
Labelled Cell Count 90 169 220 124 179 266 251 245 122 165 230 324 63 161 208 154 53 51 116 164 199 50 135 159


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 7.2 9.1 8.9 7.9 8.0 7.4 6.8 5.5 5.3 5.3 5.2 9.3 8.8 7.7 6.7 8.8 8.0 9.1 10.7 10.7
Std Deviation (1e-4) 1.5 1.3 1.6 2.4 2.4 1.4 1.7 1.5 1.1 1.0 1.0 1.0 1.6 1.5 1.6 1.1 1.9 1.3 0.7 1.7 1.9
Intensity Change (Log2) – – – -0.04 -0.21 -0.18 -0.29 -0.43 -0.72 -0.78 -0.78 -0.8 0.03 -0.05 -0.23 -0.45 -0.04 -0.18 0.01 0.23 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 3.9 3.7 3.3 2.8 1.6 1.5 1.0 -0.6 2.6 0.6 1.6 7.1 3.4 3.4
Endoplasmic Reticulum 0 0 2.5 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.2 5.4 8.9 8.6 8.8 10.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.3 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.0 -7.7 -9.6 -7.9 -8.7 -7.0 -8.8 -10.5 -9.9 -5.2 -2.1 -3.5 -9.3 -4.7 -5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.258 7.3129 6.4571 6.1626 4.1884 6.4964 10.7874 12.5899 11.0794 10.3967 11.569 12.1187 8.6302 9.8161 8.9873 9.5957 8.3471 9.4165
Actin 0.0395 0.0004 0.0212 0.0249 0.0229 0.0015 0.0358 0.0002 0.0012 0.0007 0.0002 0.0003 0.0274 0.0005 0.005 0.0023 0.0376 0.0027
Bud 0.0005 0.0001 0.0003 0.0006 0.0002 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0011 0.0002
Bud Neck 0.0005 0.0003 0.0004 0.001 0.0028 0.0011 0.0029 0.0003 0.0005 0.0005 0.0008 0.0011 0.0022 0.0004 0.0035 0.0004 0.001 0.0018
Bud Periphery 0.0006 0 0.0003 0.0007 0.0002 0.0001 0.0005 0 0.0002 0 0 0.0001 0.0003 0 0.0001 0.0001 0.0014 0.0001
Bud Site 0.0011 0.0004 0.0008 0.0006 0.0004 0.0001 0.0005 0.0003 0.0009 0.0002 0.0001 0.0001 0.0008 0.0005 0.0008 0.0008 0.0099 0.0002
Cell Periphery 0.0002 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.1968 0.2664 0.1902 0.1741 0.3183 0.2223 0.1959 0.3267 0.2256 0.3247 0.4011 0.1926 0.2898 0.2801 0.2853 0.3227 0.172 0.1835
Cytoplasmic Foci 0.0071 0.0007 0.0051 0.0033 0.0221 0.0128 0.0025 0.0007 0.0059 0.0025 0.0011 0.0008 0.0155 0.0016 0.0116 0.0024 0.0023 0.0042
Eisosomes 0.0002 0 0.0002 0 0.0003 0 0.0002 0 0 0 0 0 0.0004 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0189 0.0041 0.0095 0.0201 0.0166 0.0148 0.0128 0.0024 0.0072 0.0102 0.0104 0.0064 0.0207 0.0061 0.011 0.0091 0.0051 0.0082
Endosome 0.029 0.0013 0.0116 0.0178 0.0577 0.0155 0.0067 0.0027 0.0111 0.0104 0.0066 0.0038 0.0272 0.003 0.0121 0.0057 0.0021 0.0111
Golgi 0.0065 0 0.0024 0.0021 0.0132 0.0006 0.0009 0.0001 0.0016 0.0002 0.0001 0.0001 0.0074 0.0003 0.0025 0.0006 0.0019 0.0029
Lipid Particles 0.0083 0 0.0065 0.0012 0.068 0.0008 0.0004 0 0.0026 0.0002 0.0001 0 0.0116 0.0002 0.0069 0.0001 0.0018 0.0005
Mitochondria 0.0097 0.0003 0.0036 0.0052 0.005 0.0006 0.0013 0.0002 0.0015 0.0009 0.0003 0.0007 0.0018 0.0003 0.0013 0.0009 0.0023 0.0025
None 0.0041 0.0005 0.0025 0.0015 0.0028 0.0007 0.0135 0.0004 0.0036 0.0015 0.0013 0.0103 0.0049 0.0014 0.0075 0.0037 0.0005 0.0015
Nuclear Periphery 0.167 0.0815 0.1064 0.1549 0.0561 0.1148 0.0995 0.0461 0.0716 0.0839 0.0858 0.067 0.046 0.0673 0.0705 0.0552 0.0861 0.062
Nucleolus 0.0009 0.0005 0.0006 0.0004 0.0006 0.0006 0.0012 0.0004 0.0006 0.0002 0.0005 0.0009 0.0009 0.0005 0.0005 0.0003 0.0007 0.0008
Nucleus 0.4915 0.6382 0.6275 0.5821 0.3724 0.6023 0.6055 0.6149 0.6556 0.5552 0.4823 0.7058 0.5224 0.6325 0.5606 0.5889 0.663 0.7075
Peroxisomes 0.0025 0.0001 0.0023 0.0003 0.0278 0.0005 0.0004 0 0.0022 0.0001 0 0 0.0021 0.0001 0.0125 0.0002 0.0058 0.0003
Punctate Nuclear 0.0082 0.0027 0.0047 0.0039 0.0048 0.0054 0.0134 0.0024 0.0054 0.0039 0.0043 0.004 0.014 0.0032 0.0061 0.0049 0.0039 0.0062
Vacuole 0.004 0.0018 0.0026 0.0029 0.0044 0.0036 0.0043 0.0017 0.0016 0.0029 0.004 0.005 0.003 0.0015 0.0014 0.001 0.0009 0.0024
Vacuole Periphery 0.0031 0.0006 0.0013 0.0024 0.003 0.0019 0.0012 0.0004 0.0007 0.0015 0.001 0.0009 0.0011 0.0004 0.0006 0.0005 0.0005 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 139.4638 130.7835 134.2332 141.9406 116.1154 132.5539 156.025 157.6467 164.7983 126.8476
Translational Efficiency 1.1373 1.3215 1.185 1.1614 1.2732 1.4365 1.2285 1.02 1.2002 1.1347

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-ATPase base subunit of the 19S RP of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the regulatory particle (RP); binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rpn10

Rpn10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available