ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 0 | 0 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 85 | 81 | 27 | 53 | 113 | 156 | 218 | – | 170 | 181 | 129 | 155 | – | – | – | 101 | 77 | 65 | 22 | 18 | 7 | 3 | 6 | 14 |
Endoplasmic Reticulum | 5 | 2 | 1 | 2 | 0 | 0 | 3 | – | 2 | 4 | 4 | 2 | – | – | – | 7 | 9 | 3 | 3 | 0 | 1 | 0 | 1 | 0 |
Endosome | 2 | 4 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 0 | 1 | – | – | – | 4 | 6 | 5 | 5 | 4 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 57 | 2 | 0 | 1 | 1 | 2 | 1 | – | 7 | 5 | 34 | 19 | – | – | – | 2 | 2 | 1 | 15 | 5 | 3 | 0 | 0 | 1 |
Nucleus | 41 | 25 | 3 | 2 | 3 | 4 | 2 | – | 4 | 7 | 1 | 7 | – | – | – | 16 | 7 | 5 | 15 | 13 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 2 | – | – | – | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | – | – | – | 0 | 0 | 0 | 5 | 0 | 1 | 1 | 0 | 0 |
Vac/Vac Membrane | 179 | 140 | 31 | 24 | 80 | 62 | 46 | – | 36 | 24 | 19 | 27 | – | – | – | 70 | 43 | 52 | 241 | 170 | 92 | 0 | 4 | 6 |
Unique Cell Count | 299 | 209 | 58 | 75 | 173 | 194 | 248 | 203 | 205 | 161 | 192 | 171 | 119 | 115 | 324 | 225 | 117 | 9 | 21 | 32 | ||||
Labelled Cell Count | 375 | 254 | 62 | 82 | 198 | 224 | 271 | 221 | 222 | 191 | 214 | 202 | 144 | 132 | 324 | 225 | 117 | 9 | 21 | 32 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.6 | 5.8 | 5.2 | 6.3 | 5.1 | 4.9 | – | 4.6 | 4.6 | 4.2 | 4.6 | – | – | – | 6.5 | 6.6 | 6.7 | – | – | – |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.2 | 1.4 | 1.9 | 1.7 | 1.0 | – | 1.0 | 1.3 | 1.4 | 1.6 | – | – | – | 2.2 | 2.3 | 2.8 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.16 | 0.12 | -0.17 | -0.25 | – | -0.32 | -0.34 | -0.46 | -0.34 | – | – | – | 0.17 | 0.19 | 0.21 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 2.8 | 2.5 | 5.1 | 7.1 | – | 5.8 | 6.9 | 4.8 | 5.1 | – | – | – | 1.7 | 2.3 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | -2.5 | -1.0 | -3.0 | -5.5 | – | -5.5 | -6.9 | -6.5 | -6.2 | – | – | – | -1.7 | -2.2 | -1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3827 | -0.0114 | -0.2203 | -0.7681 | -0.3394 | -0.2745 | -0.8181 | -0.2515 | -0.52 | -0.3012 | -0.1704 | -0.143 | -1.4963 | -0.7717 | -0.9716 | -0.9145 | -1.3816 | -1.1104 |
Actin | 0.013 | 0.001 | 0.0059 | 0.0001 | 0.0237 | 0.0159 | 0.0399 | 0.0002 | 0.0056 | 0.0194 | 0.0518 | 0.0338 | 0.018 | 0.0001 | 0.0311 | 0 | 0 | 0.0001 |
Bud | 0.002 | 0.0043 | 0.0035 | 0.0004 | 0.0056 | 0.0036 | 0.0005 | 0.0015 | 0.0014 | 0.0002 | 0.0033 | 0.0004 | 0.0003 | 0.0033 | 0.0058 | 0.0002 | 0.0002 | 0.0008 |
Bud Neck | 0.0061 | 0.0008 | 0.0049 | 0.0007 | 0.0274 | 0.0033 | 0.0041 | 0.0003 | 0.0083 | 0.001 | 0.0007 | 0.0009 | 0.0002 | 0.0003 | 0.0016 | 0.0001 | 0.0002 | 0.0004 |
Bud Periphery | 0.0068 | 0.0078 | 0.0085 | 0.001 | 0.0073 | 0.0059 | 0.0007 | 0.0037 | 0.0028 | 0.0002 | 0.015 | 0.0012 | 0.0003 | 0.0015 | 0.0129 | 0.0002 | 0.0002 | 0.0004 |
Bud Site | 0.0075 | 0.0127 | 0.0122 | 0.0014 | 0.0073 | 0.0008 | 0.0067 | 0.0014 | 0.0044 | 0.0003 | 0.0018 | 0.0006 | 0.0006 | 0.0073 | 0.009 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0006 | 0.0007 | 0.0008 | 0.0003 | 0.0007 | 0.0003 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0008 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.0892 | 0.1624 | 0.1063 | 0.1419 | 0.1001 | 0.2146 | 0.0742 | 0.0873 | 0.0775 | 0.0691 | 0.1109 | 0.1559 | 0.2438 | 0.2769 | 0.2766 | 0.2153 | 0.2993 | 0.4886 |
Cytoplasmic Foci | 0.0657 | 0.0384 | 0.0543 | 0.0657 | 0.0614 | 0.0791 | 0.0437 | 0.0311 | 0.0472 | 0.0146 | 0.0125 | 0.0332 | 0.0446 | 0.0238 | 0.0332 | 0.0302 | 0.0528 | 0.051 |
Eisosomes | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0009 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0035 | 0.0027 | 0.0037 | 0.0024 | 0.0024 | 0.0027 | 0.0065 | 0.0012 | 0.0033 | 0.0082 | 0.0029 | 0.0033 | 0.0051 | 0.0031 | 0.0097 | 0.0028 | 0.0013 | 0.0032 |
Endosome | 0.0538 | 0.05 | 0.0485 | 0.0555 | 0.0812 | 0.0681 | 0.0538 | 0.0243 | 0.0398 | 0.0461 | 0.0357 | 0.0312 | 0.0376 | 0.0334 | 0.0682 | 0.2344 | 0.1511 | 0.0776 |
Golgi | 0.0074 | 0.001 | 0.0051 | 0.0009 | 0.0087 | 0.0055 | 0.0118 | 0.0007 | 0.0068 | 0.0022 | 0.005 | 0.0089 | 0.0039 | 0.001 | 0.0061 | 0.0016 | 0.0009 | 0.0026 |
Lipid Particles | 0.0157 | 0.0029 | 0.0167 | 0.0035 | 0.0168 | 0.0152 | 0.0158 | 0.0043 | 0.015 | 0.0026 | 0.0014 | 0.0114 | 0.0087 | 0.0018 | 0.0097 | 0.0005 | 0.0019 | 0.0153 |
Mitochondria | 0.0108 | 0.0024 | 0.0078 | 0.0047 | 0.0419 | 0.0082 | 0.004 | 0.0024 | 0.0096 | 0.0015 | 0.0063 | 0.0055 | 0.0014 | 0.0033 | 0.0057 | 0.0025 | 0.0012 | 0.0096 |
None | 0.648 | 0.6482 | 0.6472 | 0.6718 | 0.5565 | 0.5152 | 0.6815 | 0.7862 | 0.7044 | 0.7502 | 0.7099 | 0.6325 | 0.5562 | 0.5388 | 0.4002 | 0.2891 | 0.3591 | 0.2364 |
Nuclear Periphery | 0.0106 | 0.002 | 0.0042 | 0.0023 | 0.004 | 0.0033 | 0.0043 | 0.0009 | 0.0027 | 0.0068 | 0.0025 | 0.0073 | 0.0023 | 0.0019 | 0.0119 | 0.0075 | 0.0013 | 0.0019 |
Nucleolus | 0.0067 | 0.0022 | 0.0033 | 0.002 | 0.0047 | 0.0008 | 0.0019 | 0.0013 | 0.0013 | 0.0005 | 0.0006 | 0.0024 | 0.0005 | 0.0007 | 0.0005 | 0.0002 | 0.0004 | 0.0007 |
Nucleus | 0.0057 | 0.0056 | 0.0076 | 0.0059 | 0.0028 | 0.0069 | 0.004 | 0.0019 | 0.0027 | 0.0026 | 0.0015 | 0.0069 | 0.002 | 0.0032 | 0.0047 | 0.0027 | 0.0011 | 0.0034 |
Peroxisomes | 0.0096 | 0.0017 | 0.0108 | 0.0046 | 0.0092 | 0.0133 | 0.0143 | 0.0015 | 0.0126 | 0.0005 | 0.0003 | 0.0071 | 0.0087 | 0.0045 | 0.008 | 0.0001 | 0.0005 | 0.0026 |
Punctate Nuclear | 0.0059 | 0.0027 | 0.0093 | 0.0031 | 0.002 | 0.0041 | 0.0046 | 0.0011 | 0.0037 | 0.003 | 0.0003 | 0.0099 | 0.002 | 0.001 | 0.0029 | 0.0003 | 0.0007 | 0.001 |
Vacuole | 0.0266 | 0.0459 | 0.0331 | 0.0298 | 0.0273 | 0.028 | 0.0245 | 0.0449 | 0.0445 | 0.0558 | 0.0326 | 0.0431 | 0.059 | 0.0892 | 0.0925 | 0.1634 | 0.1001 | 0.0913 |
Vacuole Periphery | 0.0045 | 0.0045 | 0.0062 | 0.002 | 0.0084 | 0.0048 | 0.0022 | 0.0031 | 0.0056 | 0.0143 | 0.0031 | 0.0041 | 0.0044 | 0.0044 | 0.0089 | 0.0487 | 0.0275 | 0.0125 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.3547 | 4.9073 | 4.0461 | 5.1785 | 1.5817 | 3.0481 | 6.8914 | 5.5815 | 6.6039 | 5.4804 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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