Standard name | |
---|---|
Human Ortholog | |
Description | Type II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 2 | 0 | 3 | 1 | 1 | 1 | 3 | 4 | 3 | 3 | 0 | 0 | 1 | 1 | 1 |
Bud | 2 | 1 | 0 | 7 | 7 | 15 | 11 | 24 | 27 | 21 | 23 | 27 | 1 | 0 | 1 | 2 | 0 | 0 | 3 | 1 | 0 | 0 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 180 | 211 | 66 | 167 | 242 | 214 | 258 | 234 | 188 | 179 | 100 | 164 | 61 | 154 | 176 | 67 | 35 | 31 | 51 | 76 | 21 | 19 | 31 | 53 |
Endoplasmic Reticulum | 4 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 6 | 5 | 3 | 3 | 7 | 4 | 4 | 3 | 5 | 3 | 2 | 5 | 7 |
Endosome | 16 | 1 | 1 | 9 | 15 | 5 | 4 | 7 | 3 | 14 | 5 | 9 | 2 | 3 | 3 | 3 | 2 | 13 | 33 | 13 | 13 | 8 | 3 | 10 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 11 | 1 | 1 | 0 | 0 | 2 |
Mitochondria | 19 | 8 | 14 | 18 | 28 | 36 | 92 | 98 | 112 | 102 | 61 | 120 | 0 | 0 | 1 | 5 | 3 | 2 | 32 | 24 | 15 | 17 | 18 | 22 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 1 | 4 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 6 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 0 | 3 | 1 | 0 | 9 | 7 | 10 | 7 | 5 | 21 | 0 | 4 | 0 | 2 | 1 | 3 | 2 | 1 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 19 | 2 | 1 | 3 | 8 | 3 | 4 | 4 | 1 | 8 | 2 | 6 | 1 | 2 | 1 | 1 | 1 | 4 | 107 | 101 | 39 | 55 | 49 | 76 |
Unique Cell Count | 224 | 225 | 73 | 191 | 278 | 241 | 333 | 305 | 267 | 284 | 155 | 282 | 69 | 165 | 186 | 81 | 41 | 51 | 257 | 231 | 101 | 110 | 121 | 186 |
Labelled Cell Count | 250 | 230 | 82 | 209 | 302 | 278 | 386 | 383 | 350 | 345 | 201 | 371 | 72 | 168 | 188 | 90 | 51 | 64 | 257 | 231 | 101 | 110 | 121 | 186 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.6 | 3.7 | 4.0 | 4.0 | 3.9 | 3.6 | 3.6 | 3.6 | 3.7 | 3.6 | 4.0 | 5.5 | 5.3 | 5.1 | 5.4 | 5.8 | 6.3 | 5.7 | 6.1 | 6.2 |
Std Deviation (1e-4) | 1.0 | 0.7 | 0.9 | 1.9 | 1.6 | 1.6 | 1.6 | 1.3 | 1.3 | 1.4 | 1.3 | 1.4 | 1.2 | 1.3 | 1.2 | 0.8 | 1.2 | 1.2 | 1.3 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.08 | 0.07 | -0.04 | -0.04 | -0.04 | -0.02 | -0.03 | 0.09 | 0.57 | 0.5 | 0.45 | 0.53 | 0.65 | 0.75 | 0.62 | 0.72 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6686 | 0.8374 | 0.9449 | 0.5324 | 0.4236 | 1.346 | 1.6344 | 2.0651 | 2.1351 | 2.1995 | 1.8389 | 2.1623 | -0.4624 | 0.1891 | -0.1095 | 0.1415 | -0.0525 | 0.1014 |
Actin | 0.0226 | 0.003 | 0.0109 | 0.0007 | 0.0002 | 0.0131 | 0.0399 | 0.0076 | 0.0101 | 0.0139 | 0.0159 | 0.0022 | 0.0049 | 0.0011 | 0.0054 | 0.0016 | 0.0025 | 0.0019 |
Bud | 0.0004 | 0.0012 | 0.0025 | 0.0005 | 0.0004 | 0.0004 | 0.0012 | 0.0014 | 0.0008 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0002 |
Bud Neck | 0.0011 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0023 | 0.0013 | 0.0004 | 0.0005 | 0.002 | 0.0003 | 0.0012 | 0.0012 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0011 |
Bud Periphery | 0.0005 | 0.0012 | 0.0014 | 0.0008 | 0.0005 | 0.001 | 0.0026 | 0.0012 | 0.0016 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 |
Bud Site | 0.0021 | 0.0077 | 0.0068 | 0.0002 | 0.0003 | 0.0006 | 0.0028 | 0.0025 | 0.0016 | 0.0035 | 0.0007 | 0.0003 | 0.0011 | 0.0022 | 0.001 | 0.0003 | 0.0003 | 0.0002 |
Cell Periphery | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0008 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.1246 | 0.2351 | 0.1257 | 0.1506 | 0.115 | 0.2101 | 0.1118 | 0.2197 | 0.1444 | 0.0852 | 0.0239 | 0.1549 | 0.184 | 0.2963 | 0.1511 | 0.2314 | 0.1367 | 0.2297 |
Cytoplasmic Foci | 0.1508 | 0.1603 | 0.179 | 0.1846 | 0.1558 | 0.1468 | 0.2053 | 0.2412 | 0.1825 | 0.2145 | 0.2141 | 0.1708 | 0.1873 | 0.1898 | 0.1839 | 0.1373 | 0.2176 | 0.1954 |
Eisosomes | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0004 | 0.0012 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0012 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0073 | 0.01 | 0.0044 | 0.0027 | 0.0018 | 0.0054 | 0.0118 | 0.0035 | 0.0066 | 0.0027 | 0.0016 | 0.0047 | 0.0127 | 0.0074 | 0.0058 | 0.0099 | 0.0107 | 0.0086 |
Endosome | 0.3794 | 0.3003 | 0.2717 | 0.3566 | 0.4132 | 0.2612 | 0.2336 | 0.2695 | 0.2774 | 0.3931 | 0.535 | 0.3043 | 0.3512 | 0.2381 | 0.3467 | 0.3739 | 0.4606 | 0.3384 |
Golgi | 0.0517 | 0.0335 | 0.0456 | 0.0574 | 0.0177 | 0.0413 | 0.0687 | 0.1043 | 0.0906 | 0.0886 | 0.0594 | 0.0737 | 0.0561 | 0.0315 | 0.0724 | 0.0659 | 0.0624 | 0.0576 |
Lipid Particles | 0.0238 | 0.0067 | 0.0125 | 0.005 | 0.0012 | 0.018 | 0.0499 | 0.0086 | 0.0075 | 0.0169 | 0.0372 | 0.0171 | 0.0168 | 0.0094 | 0.0128 | 0.0021 | 0.008 | 0.0093 |
Mitochondria | 0.0159 | 0.0052 | 0.01 | 0.0132 | 0.0049 | 0.0098 | 0.0235 | 0.0146 | 0.0181 | 0.0184 | 0.0063 | 0.0294 | 0.0056 | 0.0073 | 0.0126 | 0.0072 | 0.0077 | 0.0081 |
None | 0.1626 | 0.1996 | 0.2801 | 0.1518 | 0.2218 | 0.2235 | 0.149 | 0.0986 | 0.2115 | 0.0985 | 0.0098 | 0.2005 | 0.1497 | 0.1888 | 0.1722 | 0.1225 | 0.0546 | 0.1255 |
Nuclear Periphery | 0.0082 | 0.0024 | 0.0059 | 0.0025 | 0.001 | 0.0071 | 0.005 | 0.0006 | 0.0012 | 0.0006 | 0.0012 | 0.0008 | 0.0026 | 0.0017 | 0.0017 | 0.002 | 0.002 | 0.0009 |
Nucleolus | 0.0006 | 0.0004 | 0.0007 | 0.0004 | 0.0001 | 0.0011 | 0.0016 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.002 | 0.0012 | 0.0014 | 0.0007 | 0.0007 | 0.0027 | 0.003 | 0.0004 | 0.0007 | 0.0005 | 0.0002 | 0.0005 | 0.0011 | 0.0011 | 0.0011 | 0.001 | 0.0009 | 0.0006 |
Peroxisomes | 0.0183 | 0.0064 | 0.0239 | 0.003 | 0.0347 | 0.015 | 0.0687 | 0.0169 | 0.0328 | 0.0497 | 0.077 | 0.028 | 0.0085 | 0.0082 | 0.018 | 0.0292 | 0.0216 | 0.0117 |
Punctate Nuclear | 0.0038 | 0.0013 | 0.004 | 0.0015 | 0.0005 | 0.0057 | 0.0029 | 0.0004 | 0.0008 | 0.0008 | 0.0029 | 0.001 | 0.0012 | 0.0011 | 0.0014 | 0.0005 | 0.0012 | 0.0007 |
Vacuole | 0.019 | 0.019 | 0.0083 | 0.0433 | 0.0243 | 0.028 | 0.0131 | 0.0062 | 0.0073 | 0.0064 | 0.0098 | 0.0062 | 0.011 | 0.0122 | 0.0089 | 0.0098 | 0.0078 | 0.007 |
Vacuole Periphery | 0.0045 | 0.0044 | 0.0038 | 0.0241 | 0.0053 | 0.0062 | 0.0024 | 0.0018 | 0.0025 | 0.0032 | 0.0038 | 0.003 | 0.0025 | 0.0023 | 0.0032 | 0.0042 | 0.0039 | 0.0029 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.8602 | 22.9925 | 11.943 | 22.2987 | 18.3412 | 7.7118 | 25.5724 | 24.3204 | 30.3043 | 25.0239 |
Translational Efficiency | 4.0284 | 2.1971 | 2.774 | 1.4234 | 2.7612 | 6.7858 | 2.0325 | 1.6574 | 1.2654 | 2.0742 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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