Standard name | |
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Human Ortholog | |
Description | Ribosome-associated protein; proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 4 | 10 | 18 | 11 | 12 | 26 | 21 | 14 | 17 | 10 | 15 | 6 | 14 | 8 | 7 | 7 | 3 | 0 | 1 | 3 | 0 | 1 | 2 |
Bud | 1 | 0 | 0 | 1 | 2 | 5 | 13 | 8 | 9 | 8 | 13 | 13 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 5 | 2 | 4 | 14 | 12 | 17 | 19 | 21 | 24 | 19 | 25 | 4 | 25 | 55 | 20 | 9 | 12 | 0 | 0 | 1 | 0 | 1 | 2 |
Cytoplasm | 9 | 11 | 13 | 31 | 42 | 25 | 52 | 65 | 58 | 79 | 53 | 86 | 4 | 8 | 30 | 31 | 9 | 10 | 4 | 3 | 2 | 1 | 4 | 5 |
Endoplasmic Reticulum | 186 | 157 | 86 | 132 | 291 | 223 | 305 | 311 | 213 | 244 | 139 | 275 | 103 | 209 | 211 | 3 | 0 | 0 | 204 | 179 | 110 | 81 | 140 | 192 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 12 | 3 | 4 | 18 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 6 | 5 | 19 | 8 | 9 | 1 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 2 | 0 | 24 | 32 | 27 | 51 | 159 | 113 | 136 | 150 | 133 | 250 | 1 | 1 | 1 | 7 | 5 | 2 | 0 | 0 | 1 | 1 | 2 | 2 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 3 | 5 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 14 | 1 | 8 | 13 | 10 | 11 | 38 | 35 | 34 | 51 | 20 | 40 | 2 | 4 | 5 | 3 | 3 | 3 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 23 | 8 | 7 | 19 | 29 | 27 | 136 | 121 | 86 | 168 | 70 | 108 | 2 | 14 | 33 | 18 | 2 | 3 | 1 | 3 | 1 | 2 | 7 | 13 |
Unique Cell Count | 209 | 175 | 117 | 198 | 363 | 287 | 547 | 556 | 450 | 589 | 359 | 640 | 108 | 237 | 282 | 99 | 38 | 36 | 215 | 191 | 123 | 91 | 169 | 224 |
Labelled Cell Count | 255 | 189 | 155 | 269 | 431 | 367 | 751 | 701 | 579 | 751 | 458 | 816 | 122 | 281 | 350 | 109 | 43 | 42 | 215 | 191 | 123 | 91 | 169 | 224 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.0 | 18.9 | 12.8 | 13.0 | 11.5 | 9.8 | 8.8 | 9.2 | 8.8 | 8.4 | 8.4 | 8.4 | 14.9 | 14.8 | 14.7 | 18.1 | 19.9 | 20.0 | 16.5 | 16.7 | 17.7 |
Std Deviation (1e-4) | 3.2 | 3.1 | 2.2 | 2.7 | 2.0 | 1.7 | 1.7 | 1.7 | 1.6 | 1.6 | 1.6 | 1.5 | 2.4 | 2.7 | 2.4 | 4.5 | 4.0 | 4.5 | 2.5 | 2.8 | 3.2 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.15 | -0.38 | -0.53 | -0.47 | -0.54 | -0.61 | -0.6 | -0.6 | 0.22 | 0.21 | 0.2 | 0.5 | 0.64 | 0.65 | 0.37 | 0.39 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | -2.5 | -1.8 | -1.6 | -2.2 | 0 | 0 | 0 | 0 | -0.9 | -0.9 | -2.5 | -0.4 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 2.9 | 4.6 | 4.5 | 0 | 0 |
Cytoplasm | 1.1 | 0.1 | -0.7 | -0.5 | 0.2 | 0.5 | 0.7 | 1.0 | 0.7 | -2.1 | -2.9 | -0.1 | 3.7 | 1.9 | 2.4 |
Endoplasmic Reticulum | -1.3 | 1.5 | 0.9 | -3.5 | -3.5 | -5.1 | -6.4 | -6.5 | -6.1 | 4.5 | 3.5 | 0.3 | -10.5 | -7.9 | -7.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 |
Mitochondria | -1.0 | -4.0 | -0.6 | 1.9 | 0 | 2.1 | 1.1 | 3.3 | 3.8 | -4.7 | -6.9 | -7.6 | -2.8 | -1.0 | -2.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.1 | 0 | -1.3 | 0 | -0.2 | 0.3 | 0.7 | -0.5 | -0.2 | 0 | 0 | 0 | -1.3 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.1 | 0.7 | 1.1 | 4.5 | 4.0 | 3.4 | 5.2 | 3.4 | 3.0 | 0 | 0 | 1.7 | 2.8 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0009 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0638 | 0.0002 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | – | – | – | – | – | – |
Bud Neck | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0001 | – | – | – | – | – | – |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0 | – | – | – | – | – | – |
Bud Site | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0003 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0007 | 0.001 | 0.0009 | 0.0004 | 0.0007 | 0.0003 | 0.0003 | 0.0024 | 0.0009 | 0.0004 | 0.0003 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.01 | 0.0293 | 0.0189 | 0.0037 | 0.004 | 0.0104 | 0.0095 | 0.0192 | 0.0033 | 0.0279 | 0.0015 | 0.0017 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0114 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0011 | 0.0149 | 0.0006 | 0.0002 | 0.0007 | 0.0104 | 0.0027 | – | – | – | – | – | – |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.9246 | 0.9518 | 0.9661 | 0.9872 | 0.9832 | 0.9738 | 0.8256 | 0.9518 | 0.9741 | 0.9634 | 0.9388 | 0.9792 | – | – | – | – | – | – |
Endosome | 0.0087 | 0.0013 | 0.0033 | 0.0008 | 0.0013 | 0.0032 | 0.038 | 0.0015 | 0.0032 | 0.0014 | 0.0031 | 0.0039 | – | – | – | – | – | – |
Golgi | 0.001 | 0.0002 | 0.0013 | 0 | 0.0001 | 0.0004 | 0.0099 | 0.0006 | 0.001 | 0.0002 | 0.0011 | 0.0053 | – | – | – | – | – | – |
Lipid Particles | 0.0048 | 0.0014 | 0.0028 | 0.0001 | 0.0006 | 0.0011 | 0.0103 | 0.0142 | 0.001 | 0.0003 | 0.0047 | 0.0002 | – | – | – | – | – | – |
Mitochondria | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0.003 | 0 | 0.0002 | 0.0005 | – | – | – | – | – | – |
None | 0.0012 | 0 | 0 | 0 | 0 | 0.0002 | 0.0036 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | – | – | – | – | – | – |
Nuclear Periphery | 0.0249 | 0.011 | 0.0045 | 0.0058 | 0.0067 | 0.0055 | 0.0097 | 0.0051 | 0.0082 | 0.0033 | 0.0023 | 0.004 | – | – | – | – | – | – |
Nucleolus | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Nucleus | 0.0026 | 0.001 | 0.0002 | 0.0003 | 0.0006 | 0.0008 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | – | – | – | – | – | – |
Peroxisomes | 0.0029 | 0 | 0 | 0 | 0 | 0.0001 | 0.0054 | 0 | 0 | 0 | 0.0359 | 0.0009 | – | – | – | – | – | – |
Punctate Nuclear | 0.0024 | 0 | 0 | 0 | 0 | 0.0006 | 0.0008 | 0 | 0 | 0 | 0.0002 | 0 | – | – | – | – | – | – |
Vacuole | 0.0025 | 0.002 | 0.0011 | 0.0013 | 0.002 | 0.0014 | 0.0038 | 0.0036 | 0.0022 | 0.0009 | 0.0009 | 0.0008 | – | – | – | – | – | – |
Vacuole Periphery | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0012 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 47.1246 | 84.253 | 64.0442 | 59.3754 | 58.1186 | 68.9246 | 58.6967 | 58.8986 | 73.9334 | 54.385 |
Translational Efficiency | 1.3244 | 1.0133 | 1.2607 | 1.6335 | 1.2877 | 0.934 | 1.2176 | 1.2537 | 0.9308 | 1.2082 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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