Standard name
Human Ortholog
Description Membrane-embedded ubiquitin-protein ligase; ER and inner nuclear membrane localized RING-CH domain E3 ligase involved in ER-associated protein degradation (ERAD); targets misfolded cytosolic/nucleoplasmic domains of soluble and membrane embedded proteins (ERAD-C) and a transmembrane domain containing substrate (ERAD-M), Sbh2p; C-terminal element (CTE), conserved in human ortholog MARCH10/TEB4, determines substrate selectivity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 – 0 0 0 0.05 0.06 0.07 0.05 0 0.06 0 0 0 0 0 0
Cytoplasm 0.24 0.36 0.24 0.23 0.35 0.38 0.45 0.45 – 0.54 0.45 0.45 0.26 0.34 0.47 0.69 0.65 0.6 0 0.1 0 0 0 0
Endoplasmic Reticulum 0.5 0.55 0.53 0.37 0.12 0.09 0 0 – 0 0 0 0.72 0.56 0.48 0 0 0.06 0.85 0.67 0.83 0.64 0.77 0.79
Endosome 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.17 0.39 0.27 0.6 0.61 0.65 – 0.62 0.69 0.68 0 0 0 0.15 0.11 0.08 0 0.06 0 0.15 0.07 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.29 0.06 0.17 0.12 0.32 0.11 0.11 0.09 – 0.05 0.05 0 0.05 0.09 0.07 0.15 0.16 0.2 0 0.05 0 0.06 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 27 17 9 1 1 0 0 – 0 0 0 7 8 5 5 13 9 9 19 20 14 15 17
Bud 2 10 6 8 3 13 18 19 – 16 24 27 0 1 1 2 4 6 2 5 2 6 3 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 2 0 0 1 0 2 5 0 2 5 3 3 1 2
Bud Site 0 1 0 0 0 0 0 1 – 8 6 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 14 9 33 16 22 9 5 8 – 2 10 6 18 20 21 16 13 13 3 6 11 13 8 10
Cytoplasm 107 160 126 94 172 260 342 388 – 366 257 320 92 112 146 227 236 121 22 54 18 20 16 18
Endoplasmic Reticulum 225 246 283 149 58 65 8 6 – 5 5 5 254 181 148 11 15 12 480 354 545 355 495 584
Endosome 16 3 21 7 30 3 11 5 – 2 1 2 4 3 1 9 10 5 3 7 2 4 2 7
Golgi 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3 3 2 5 4 3
Mitochondria 8 32 93 157 131 413 460 564 – 421 398 486 1 1 0 49 41 16 18 33 26 81 44 31
Nucleus 3 0 2 1 6 2 5 11 – 9 8 6 0 0 4 6 17 4 0 1 0 0 1 0
Nuclear Periphery 3 2 6 11 7 5 3 4 – 5 7 5 0 1 0 1 5 1 3 3 4 4 6 7
Nucleolus 0 0 0 1 0 0 1 2 – 0 1 3 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 1 0 4 4 – 3 3 4 0 0 0 4 2 0 0 1 0 1 0 0
Vac/Vac Membrane 128 28 89 47 159 75 84 74 – 36 27 31 19 28 23 48 57 40 12 27 8 34 34 50
Unique Cell Count 449 450 535 401 490 685 755 866 679 577 717 354 325 310 327 361 203 565 526 654 552 642 744
Labelled Cell Count 515 518 679 501 591 846 941 1087 873 749 900 395 356 349 380 418 228 565 526 654 552 642 744


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 6.2 6.3 5.8 5.4 4.0 3.6 3.6 – 3.1 3.1 3.2 8.1 7.8 7.9 5.6 6.1 7.2 7.5 8.3 8.6
Std Deviation (1e-4) 1.0 1.1 1.2 1.2 1.4 1.1 1.1 0.8 – 1.0 1.0 0.9 0.9 1.0 1.1 1.0 1.2 1.5 1.6 1.6 1.5
Intensity Change (Log2) – – – -0.11 -0.23 -0.64 -0.82 -0.79 – -1.01 -1.01 -0.97 0.36 0.31 0.33 -0.17 -0.03 0.19 0.26 0.4 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -3.6 0 0 0 – 0 0 0 -1.1 -0.6 -1.4 -1.5 0.3 0.8
Bud 1.1 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -1.5 -1.2 0 0 0 – 0 0 0 -0.7 0 0.3 -0.8 -1.7 0.1
Cytoplasm 0 4.1 5.4 8.0 8.0 – 10.7 7.4 7.7 0.8 3.5 7.1 13.3 12.5 9.3
Endoplasmic Reticulum -4.8 -13.9 0 0 0 – 0 0 0 5.6 0.8 -1.4 -14.9 -15.2 -11.6
Endosome -1.9 1.6 0 0 0 – 0 0 0 -2.5 -2.6 -3.2 -0.9 -0.9 -1.0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 7.4 3.6 0 0 0 – 0 0 0 -8.1 -7.8 -7.8 -0.9 -2.5 -3.2
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 4.4 0
Nuclear Periphery 1.8 0.4 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -2.1 5.9 -2.9 -2.9 -4.6 – -6.5 -6.5 -7.3 -5.0 -3.3 -3.8 -0.8 -0.3 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4904 5.557 6.0641 5.5079 5.3247 5.8548 7.5279 8.3054 7.7381 7.7296 7.2265 8.1775 8.3519 8.8097 7.9841 7.7333 8.1451 8.1821
Actin 0.0114 0.0089 0.003 0.0005 0.0038 0.0016 0.0439 0.0011 0.0225 0.0001 0.004 0.0005 0.0329 0.0009 0.0083 0.0103 0.0013 0.002
Bud 0.0001 0.0006 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0015 0
Bud Neck 0.0005 0.0008 0.0007 0.001 0.0002 0.0016 0.0006 0.0002 0.0004 0.0003 0.0013 0.0004 0.0012 0.0016 0.0002 0.0071 0.0011 0.0006
Bud Periphery 0.0002 0.0008 0.0002 0.0002 0.0001 0.0001 0.0005 0 0.0002 0 0.0003 0.0002 0.0006 0.0003 0.0001 0.0005 0.0023 0
Bud Site 0.0004 0.0104 0.0009 0.0004 0.0001 0.0002 0.0009 0.0015 0.0009 0 0.0022 0 0.0028 0.002 0.0006 0.0012 0.0077 0.0002
Cell Periphery 0.0005 0.0018 0.001 0.0016 0.0004 0.0003 0.0006 0.001 0.0003 0.0002 0.0011 0.0003 0.0009 0.0035 0.0003 0.0011 0.0009 0.0002
Cytoplasm 0.1389 0.2891 0.1072 0.0517 0.016 0.0932 0.0118 0.0539 0.0201 0.0064 0.0142 0.0375 0.0328 0.0829 0.0195 0.0249 0.0586 0.0175
Cytoplasmic Foci 0.0098 0.012 0.0123 0.0007 0.0395 0.0031 0.0219 0.0027 0.0095 0.0001 0.0076 0.0009 0.0167 0.0045 0.0073 0.0051 0.0044 0.0004
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0006 0 0.0001 0 0.0011 0 0.0003 0.0001 0.0001 0.0006 0 0.0001
Endoplasmic Reticulum 0.658 0.488 0.7749 0.8632 0.8509 0.7948 0.7532 0.9148 0.8918 0.9725 0.8676 0.9264 0.7236 0.8174 0.87 0.8967 0.8701 0.9469
Endosome 0.0325 0.0436 0.0239 0.0049 0.0108 0.0172 0.0458 0.0061 0.0158 0.0014 0.0152 0.0039 0.0396 0.0175 0.0216 0.013 0.0183 0.0038
Golgi 0.0037 0.0046 0.0037 0.0004 0.0018 0.0015 0.01 0.001 0.0051 0.0001 0.0021 0.0003 0.0081 0.004 0.0126 0.0111 0.0047 0.0004
Lipid Particles 0.0075 0.0052 0.0068 0.0021 0.004 0.0029 0.0213 0.0026 0.0075 0.0003 0.0209 0.0008 0.0164 0.0113 0.0119 0.0091 0.0021 0.0005
Mitochondria 0.0039 0.0011 0.0102 0.0004 0.0006 0.0006 0.0034 0.0001 0.0029 0.0001 0.0011 0.0001 0.0023 0.0183 0.0244 0.0022 0.0015 0.0005
None 0.0108 0.0049 0.0078 0.0002 0.0008 0.0025 0.0047 0.0004 0.0018 0 0.0231 0.0091 0.0098 0.0062 0.0004 0.0022 0.0004 0.0073
Nuclear Periphery 0.0772 0.0658 0.0291 0.0267 0.0377 0.0574 0.0547 0.0096 0.0117 0.0152 0.0135 0.0146 0.0614 0.0182 0.0108 0.0054 0.0065 0.0149
Nucleolus 0.0004 0.0005 0.0001 0.0004 0.0001 0.0001 0.0003 0 0.0002 0 0.0009 0 0.0004 0.0001 0.0001 0.0004 0.0009 0
Nucleus 0.0252 0.034 0.0079 0.0336 0.0061 0.0138 0.0028 0.001 0.001 0.0009 0.0029 0.0019 0.0149 0.0016 0.0008 0.0011 0.0029 0.002
Peroxisomes 0.0022 0.0005 0.001 0 0.0179 0.0001 0.0066 0.0001 0.0036 0 0.0096 0 0.0201 0.0003 0.007 0.0026 0.0003 0
Punctate Nuclear 0.0039 0.0055 0.0015 0.0008 0.0034 0.0019 0.0077 0.0002 0.0016 0 0.0056 0.0004 0.0037 0.0004 0.0002 0.0008 0.0007 0.0001
Vacuole 0.0103 0.0187 0.0063 0.0106 0.0047 0.0057 0.006 0.0033 0.0024 0.002 0.0046 0.0021 0.0084 0.008 0.0029 0.004 0.0128 0.0021
Vacuole Periphery 0.0025 0.0029 0.0015 0.0006 0.0012 0.0014 0.0026 0.0002 0.0006 0.0004 0.0007 0.0003 0.0029 0.0008 0.0009 0.0005 0.0011 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.6687 45.5825 46.5776 58.0518 42.4487 45.1143 59.0587 63.4031 52.8381 58.3514
Translational Efficiency 0.7193 0.7852 0.8796 0.5109 0.6467 1.0085 0.6889 0.5681 0.6965 0.6287

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Membrane-embedded ubiquitin-protein ligase; ER and inner nuclear membrane localized RING-CH domain E3 ligase involved in ER-associated protein degradation (ERAD); targets misfolded cytosolic/nucleoplasmic domains of soluble and membrane embedded proteins (ERAD-C) and a transmembrane domain containing substrate (ERAD-M), Sbh2p; C-terminal element (CTE), conserved in human ortholog MARCH10/TEB4, determines substrate selectivity
Localization
Cell Percentages ER (61%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ssm4

Ssm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available