Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) transcription factor from ATF/CREB family involved in stress-responsive regulatory network; mediates transcriptional activation of NCE103 in response to low CO2 levels; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0.1 0.18 0.14 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0 0 0 0.06 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.96 1.0 0.94 0.93 0.92 0.93 0.89 0.86 0.88 0.84 0.87 0.89 0.83 0.82 0.88 0.87 0.85 0.92 0.95 0.91 0.95 0.89 0.83
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.05 0.11 0.16 0.1 0.19 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3 2 2 0 2 0 0 0 0 0 0 1 2 1 1 1 0
Bud Neck 0 0 0 0 0 0 0 4 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 1 1 0 2 3 5 2 8 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 11 0 11 18 13 20 22 2 15 8 8 9 31 35 14 11 13 0 1 0 1 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 5 1 6 0 0 0 0 0 0
Endosome 0 1 0 0 0 1 1 0 0 0 0 1 0 0 2 0 1 1 1 1 0 0 1 1
Golgi 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 1 0 0 3 0 0 0 1 0
Mitochondria 12 4 0 0 1 3 5 12 20 12 38 18 0 0 0 4 8 8 1 1 0 0 0 1
Nucleus 241 410 69 215 321 316 477 671 307 320 270 366 77 144 200 289 197 268 238 413 81 82 133 139
Nuclear Periphery 2 1 0 1 0 3 10 2 4 1 1 2 0 0 0 1 1 3 0 0 0 0 0 0
Nucleolus 11 1 1 0 3 17 24 86 58 38 61 91 0 1 5 8 6 14 2 2 0 0 0 4
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 0 3 0 1 1 1 1 1 2 0 1 1 1 0 0 0 1 1
Vac/Vac Membrane 1 8 0 0 4 6 7 8 8 3 2 4 1 0 2 16 10 22 7 8 3 0 5 12
Unique Cell Count 251 429 69 228 346 345 515 756 358 362 320 422 87 174 244 327 226 316 259 435 89 87 149 168
Labelled Cell Count 271 437 70 230 348 361 544 813 404 399 383 501 88 178 250 338 236 337 259 435 89 87 149 168


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.3 6.9 8.4 8.9 7.9 8.2 8.7 7.6 8.3 7.1 7.6 8.5 8.6 8.0 11.4 11.5 10.6 7.3 8.1 8.5
Std Deviation (1e-4) 1.5 1.4 1.3 2.1 1.6 1.7 1.6 1.7 1.8 2.0 1.8 1.8 1.6 1.9 1.9 2.6 3.1 3.2 1.4 1.6 1.5
Intensity Change (Log2) – – – 0.28 0.36 0.18 0.23 0.33 0.13 0.25 0.03 0.13 0.3 0.31 0.2 0.71 0.72 0.61 0.07 0.23 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 3.8 3.3 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.0 0 0 0 0 0 0 0
Nucleus -2.0 -2.3 -2.5 -2.3 -2.9 -3.3 -3.0 -3.5 -3.2 -2.9 -3.7 -3.8 -3.0 -3.1 -3.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.6 3.3 2.4 3.6 4.0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7056 6.1159 6.2063 5.5773 5.2218 5.6392 4.3097 4.5277 4.0501 3.6183 3.1561 4.4385 3.8492 2.833 3.4435 3.3711 3.3328 3.4267
Actin 0 0 0 0 0 0 0.014 0 0.0176 0.0289 0.003 0.0018 0.035 0 0 0 0 0
Bud 0 0 0 0 0 0 0.0009 0 0.0004 0.0004 0.001 0.0002 0.0001 0.0001 0 0 0 0.0002
Bud Neck 0 0 0 0 0.0007 0.0002 0.0012 0.0001 0.0013 0.0188 0.0009 0.0009 0.0004 0.0001 0 0 0 0.0005
Bud Periphery 0 0 0.0001 0 0 0 0.0028 0 0.0005 0.0008 0.0035 0.0006 0.0001 0 0 0 0 0.0002
Bud Site 0 0 0 0 0 0 0.0061 0.0002 0.0038 0.0073 0.0013 0.0005 0.0002 0.0004 0 0 0 0.0004
Cell Periphery 0 0 0 0 0 0 0.0007 0 0.0003 0.0007 0.0009 0.0002 0.0001 0 0 0 0 0.0001
Cytoplasm 0.0004 0.0004 0 0 0 0.0001 0.0054 0.0031 0.006 0.0003 0.0032 0.0009 0.0018 0.0011 0 0 0 0.0001
Cytoplasmic Foci 0.0003 0 0 0 0 0.0001 0.0372 0.0001 0.0061 0.0271 0.0063 0.0027 0.0083 0.0001 0 0 0 0
Eisosomes 0 0 0 0 0 0 0.0004 0 0.0022 0.0003 0.0002 0.0001 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0004 0 0 0 0 0 0.0035 0.0003 0.0026 0.0002 0.0026 0.0007 0.0024 0.0001 0 0 0 0
Endosome 0.0008 0 0 0 0 0.0004 0.0136 0.0011 0.0088 0.0015 0.009 0.0037 0.0047 0.0003 0 0 0 0
Golgi 0 0 0 0 0 0 0.0053 0 0.0062 0.0025 0.0013 0.0012 0.003 0 0 0 0 0
Lipid Particles 0.0003 0 0 0 0 0.0002 0.0258 0 0.0045 0.0026 0.0047 0.005 0.0151 0 0 0 0 0
Mitochondria 0.0001 0 0.0009 0 0.0001 0 0.011 0.0002 0.0119 0.0021 0.0359 0.0033 0.001 0.0002 0.0001 0 0 0.0001
None 0.0001 0 0 0 0 0.0001 0.008 0.0005 0.0033 0.0003 0.0084 0.0035 0.0017 0.0004 0 0 0 0
Nuclear Periphery 0.0016 0.0028 0.0012 0.0001 0.0004 0.0009 0.0143 0.0092 0.0033 0.0008 0.0297 0.0022 0.0027 0.008 0.0024 0.0001 0.0002 0.0001
Nucleolus 0.0087 0.0072 0.0049 0.0028 0.0409 0.0273 0.0131 0.0085 0.0052 0.0093 0.0655 0.0231 0.0099 0.0167 0.006 0.0026 0.0125 0.0403
Nucleus 0.9815 0.9887 0.9858 0.997 0.9575 0.9683 0.7879 0.9709 0.8976 0.8829 0.7763 0.9438 0.9029 0.9649 0.9913 0.9972 0.9869 0.9576
Peroxisomes 0 0 0 0 0 0 0.0122 0 0.0107 0.0113 0.0029 0.0005 0.0032 0 0 0 0 0
Punctate Nuclear 0.0053 0.0007 0.0051 0 0.0003 0.0021 0.0246 0.0011 0.004 0.0007 0.028 0.0034 0.0068 0.0038 0.0001 0 0.0002 0.0002
Vacuole 0.0003 0.0001 0.0006 0 0.0001 0.0001 0.0074 0.0037 0.0024 0.0008 0.0057 0.001 0.0002 0.003 0 0 0 0.0002
Vacuole Periphery 0.0001 0.0001 0.0011 0 0 0 0.0044 0.0009 0.001 0.0002 0.0098 0.0006 0.0002 0.0007 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.6419 13.9793 17.8419 26.9464 22.7081 24.3815 28.8342 37.2051 28.9818 27.735
Translational Efficiency 1.3402 1.759 1.52 1.045 1.0652 1.6485 1.2413 0.9992 1.4675 1.2775

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) transcription factor from ATF/CREB family involved in stress-responsive regulatory network; mediates transcriptional activation of NCE103 in response to low CO2 levels; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Cst6

Cst6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cst6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available