Standard name
Human Ortholog
Description GPI-glycan remodelase; conserved phosphoesterase domain-containing protein; acts together with Emp24p/Erv25p in cargo exit from the ER; functional ortholog of mammalian GPI-glycan remodelase PGAP5; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.11 0 0 0 0 0 0
Cytoplasm 0.77 0.84 1.0 0.81 0.82 0.61 0.65 0.65 0.53 0.64 0.43 0.45 0.9 0.96 0.93 0.66 0.49 0.62 0.47 0.58 0.44 0.61 0.67 0.59
Endoplasmic Reticulum 0 0.09 0 0.07 0.05 0 0 0 0.05 0 0 0 0.07 0 0 0 0.05 0.11 0.19 0.16 0.15 0.2 0.09 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0 0.17 0.16 0.26 0.44 0.53 0.48 0.62 0.42 0.77 0.69 0 0 0 0.06 0.08 0.09 0.16 0.08 0.23 0.06 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.19 0.29 0.11 0 0.05 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 4 4 5 0 1 2 6 9 9
Bud 0 1 0 0 2 1 1 2 3 0 2 2 0 1 1 1 0 1 2 2 0 3 10 11
Bud Neck 0 0 0 0 0 2 0 0 0 0 1 0 1 0 0 0 2 0 0 1 0 3 9 11
Bud Site 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 0 0 1 2 2 2 8 4 7 17 13 10 0 2 0 2 6 10
Cytoplasm 41 90 18 35 32 68 60 116 61 47 48 29 290 354 354 128 71 56 45 80 21 145 241 204
Endoplasmic Reticulum 1 10 0 3 2 5 4 2 6 1 1 0 22 10 14 8 7 10 17 22 7 48 33 25
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 6 5 1 2 1 0 0 4 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 1 1 0 1 1 1 1
Mitochondria 11 2 3 7 10 49 49 85 71 31 85 45 2 3 1 12 11 8 15 11 11 15 21 21
Nucleus 1 1 0 0 0 1 0 2 0 1 1 1 1 0 2 2 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 1 0 0 2 1 1 1 0 1 0 0 1 0 1 3 0 0 0 0
Nucleolus 1 1 0 1 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 2 1 3 1 0 2 0 0 1 1 1 2 1 1 0 2 9 10
Vac/Vac Membrane 5 3 0 1 2 1 3 4 4 2 2 2 6 1 7 37 42 10 4 6 0 2 5 17
Unique Cell Count 53 107 18 43 39 112 93 178 115 74 111 65 322 367 382 195 144 91 95 140 49 240 363 344
Labelled Cell Count 60 109 21 48 48 129 119 213 153 86 143 85 333 376 389 218 160 106 95 140 49 240 363 344


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.4 4.1 3.9 3.9 3.6 3.2 3.4 3.4 6.2 3.1 3.3 4.5 4.4 4.7 6.7 8.1 7.4 4.4 4.4 5.2
Std Deviation (1e-4) 1.0 1.2 2.6 1.6 1.5 1.5 1.6 1.6 1.9 9.1 1.4 1.9 1.3 1.2 1.7 1.4 1.7 1.7 1.8 1.8 1.7
Intensity Change (Log2) – – – -0.08 -0.06 -0.19 -0.36 -0.27 -0.25 0.61 -0.39 -0.3 0.12 0.09 0.19 0.72 0.99 0.85 0.09 0.1 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9902 1.3109 1.5202 1.0501 1.3056 1.2665 2.2282 2.2578 2.7056 2.0506 2.6207 2.4064 5.22 3.7846 3.2473 4.1901 4.4989 2.5492
Actin 0.049 0.0008 0.0466 0.0006 0.0415 0.0072 0.0028 0.0155 0.0181 0 0.0541 0.0301 0.1897 0 0.0083 0.0004 0.0003 0.0127
Bud 0.0005 0.0019 0.0114 0.0002 0.0001 0.0007 0.0001 0.0032 0.0003 0 0.0004 0.0004 0.0032 0 0 0.0001 0.1188 0.0031
Bud Neck 0.0031 0.0004 0.0007 0.0001 0.0005 0.0033 0.0001 0.0001 0.0003 0 0.0002 0.0004 0.0097 0 0.0001 0.0035 0.001 0.0301
Bud Periphery 0.0007 0.0051 0.0111 0.0007 0.0002 0.002 0.0001 0.0071 0.0005 0 0.0008 0.0007 0.0092 0 0.0001 0.0006 0.0457 0.0142
Bud Site 0.0082 0.0118 0.0035 0.0001 0.0003 0.0016 0.0003 0.009 0.0025 0 0.0002 0.0004 0.0046 0 0.0001 0.0005 0.0128 0.0012
Cell Periphery 0.0013 0.0012 0.0013 0.0004 0.0002 0.0003 0.0002 0.0008 0.0006 0 0.0001 0.0002 0.0014 0.0001 0.0001 0.0073 0.003 0.0005
Cytoplasm 0.1072 0.1869 0.1589 0.1513 0.0885 0.1557 0.1768 0.1152 0.1543 0.0009 0.0956 0.2056 0.0044 0.1067 0.0693 0.3111 0.0375 0.1607
Cytoplasmic Foci 0.0466 0.0121 0.0116 0.0062 0.0033 0.0119 0.0041 0.0052 0.0326 0.0002 0.0016 0.0159 0.0054 0.001 0.0028 0.0042 0.0092 0.0055
Eisosomes 0.0038 0.0002 0.0004 0.0002 0.0003 0.0002 0.0002 0.0004 0.0006 0 0.0004 0.0001 0.0019 0.0002 0.0003 0.0004 0 0.0002
Endoplasmic Reticulum 0.0923 0.0497 0.0836 0.1319 0.1645 0.0402 0.1922 0.0646 0.052 0 0.1786 0.0501 0.0345 0.0064 0.1842 0.0949 0.0057 0.1002
Endosome 0.0154 0.0084 0.0049 0.0078 0.0231 0.0065 0.0199 0.0036 0.0205 0 0.0029 0.0285 0.0728 0.0003 0.0044 0.0049 0.061 0.0015
Golgi 0.0052 0.0007 0.0022 0.0005 0.0065 0.0023 0.0012 0.0075 0.0077 0 0.0113 0.0134 0.0312 0 0.0071 0.0003 0.0029 0.0003
Lipid Particles 0.0352 0.0054 0.0037 0.0027 0.0085 0.0095 0.0011 0.0018 0.0062 0 0.0007 0.0114 0.0215 0.0002 0.0009 0.0055 0.0036 0.0006
Mitochondria 0.0037 0.0012 0.0095 0.0011 0.0014 0.0078 0.0007 0.0015 0.0329 0 0.0179 0.0202 0.0267 0.0001 0.0689 0.0009 0.0026 0.0009
None 0.4936 0.6844 0.6206 0.6756 0.6289 0.7163 0.57 0.7548 0.6474 0.9988 0.6325 0.591 0.5021 0.8835 0.6221 0.5399 0.6084 0.6603
Nuclear Periphery 0.0341 0.0091 0.0048 0.0129 0.0244 0.0162 0.0142 0.0041 0.0082 0 0.0007 0.0061 0.0489 0.0003 0.0109 0.0042 0.002 0.0026
Nucleolus 0.0128 0.001 0.0006 0.0003 0.0002 0.0006 0.0003 0.0003 0.0003 0 0 0.0001 0.0011 0.0001 0.0001 0.0006 0.0014 0.0002
Nucleus 0.0285 0.0068 0.0033 0.0021 0.0014 0.0067 0.007 0.0011 0.0011 0 0.0004 0.0015 0.0042 0.0002 0.0034 0.0041 0.0045 0.002
Peroxisomes 0.0314 0.0023 0.0152 0.0003 0.0005 0.0033 0.0002 0.0008 0.0081 0 0.0007 0.0202 0.0037 0 0.0134 0.0001 0.0003 0.0004
Punctate Nuclear 0.022 0.0057 0.0031 0.001 0.0033 0.0059 0.0062 0.0013 0.0018 0 0.0002 0.0012 0.0038 0.0001 0.0002 0.0007 0.0006 0.0014
Vacuole 0.0042 0.0047 0.0024 0.0032 0.0012 0.0014 0.0017 0.0017 0.0026 0 0.0004 0.0016 0.0095 0.0005 0.0028 0.0152 0.0735 0.0012
Vacuole Periphery 0.0013 0.0004 0.0005 0.0007 0.0011 0.0005 0.0006 0.0004 0.0014 0 0.0002 0.0007 0.0105 0 0.0005 0.0004 0.0049 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.5516 47.1685 59.7077 66.2617 66.9027 51.1845 66.2779 62.9577 68.4448 70.0485
Translational Efficiency 1.0089 1.2201 0.9612 0.7998 0.7837 1.2686 0.9448 0.8706 0.7831 0.7788

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GPI-glycan remodelase; conserved phosphoesterase domain-containing protein; acts together with Emp24p/Erv25p in cargo exit from the ER; functional ortholog of mammalian GPI-glycan remodelase PGAP5; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
Localization
Cell Percentages cytoplasm (40%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ted1

Ted1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ted1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available