Standard name | |
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Human Ortholog | |
Description | Cytochrome b reductase; not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 7 | 10 | 16 | 15 | 13 | 11 | 15 | 15 | 8 | 11 | 22 | 10 | 3 | 5 | 15 | 13 | 19 | 2 | 3 | 3 | 1 | 2 | 4 |
Bud | 3 | 1 | 0 | 1 | 0 | 2 | 15 | 6 | 8 | 5 | 9 | 6 | 3 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 1 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 6 | 10 | 24 | 22 | 35 | 10 | 17 | 22 | 18 | 12 | 22 | 28 | 38 | 53 | 39 | 40 | 46 | 1 | 1 | 3 | 3 | 4 | 5 |
Cytoplasm | 22 | 27 | 15 | 30 | 27 | 49 | 39 | 61 | 71 | 63 | 44 | 49 | 27 | 24 | 56 | 51 | 51 | 28 | 0 | 7 | 3 | 4 | 4 | 3 |
Endoplasmic Reticulum | 117 | 173 | 139 | 115 | 135 | 141 | 121 | 114 | 94 | 110 | 77 | 98 | 153 | 139 | 123 | 1 | 3 | 3 | 166 | 192 | 171 | 120 | 173 | 156 |
Endosome | 3 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 12 | 9 | 7 | 0 | 1 | 1 | 1 | 1 | 1 |
Mitochondria | 15 | 2 | 0 | 13 | 11 | 48 | 65 | 51 | 84 | 61 | 70 | 61 | 5 | 0 | 1 | 4 | 5 | 9 | 2 | 3 | 6 | 5 | 3 | 6 |
Nucleus | 0 | 1 | 3 | 1 | 1 | 2 | 5 | 5 | 3 | 4 | 3 | 2 | 3 | 4 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 4 | 7 | 0 | 2 | 4 | 11 | 2 | 3 | 5 | 5 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 3 | 2 | 6 | 45 | 47 | 55 | 61 | 37 | 37 | 10 | 42 | 17 | 17 | 47 | 23 | 12 | 5 | 2 | 6 | 2 | 4 | 7 | 10 |
Unique Cell Count | 156 | 203 | 160 | 168 | 209 | 254 | 268 | 271 | 262 | 252 | 193 | 250 | 211 | 179 | 235 | 124 | 116 | 108 | 180 | 222 | 200 | 145 | 204 | 194 |
Labelled Cell Count | 183 | 224 | 187 | 208 | 260 | 342 | 334 | 333 | 338 | 311 | 241 | 307 | 249 | 225 | 291 | 147 | 137 | 123 | 180 | 222 | 200 | 145 | 204 | 194 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 12.8 | 9.3 | 9.3 | 9.4 | 8.3 | 7.6 | 8.1 | 7.1 | 7.2 | 6.9 | 7.5 | 10.6 | 10.8 | 10.6 | 13.6 | 15.0 | 14.5 | 11.4 | 11.6 | 11.8 |
Std Deviation (1e-4) | 1.4 | 1.9 | 1.2 | 1.3 | 1.3 | 1.4 | 1.1 | 1.4 | 1.2 | 1.3 | 1.2 | 1.2 | 1.5 | 1.5 | 1.4 | 2.5 | 3.3 | 2.7 | 1.7 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.01 | -0.16 | -0.29 | -0.19 | -0.39 | -0.36 | -0.43 | -0.32 | 0.2 | 0.22 | 0.19 | 0.55 | 0.69 | 0.64 | 0.29 | 0.32 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.1 | 0.4 | -0.5 | -1.0 | -0.3 | -0.2 | -1.5 | -0.2 | 0.9 | -0.6 | -2.2 | -2.1 | 1.7 | 1.5 | 2.9 |
Bud | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.4 | 1.4 | 2.4 | -1.2 | 0 | 0.8 | 0.4 | 0 | 0.9 | 2.2 | 3.9 | 4.3 | 5.6 | 6.0 | 7.2 |
Cytoplasm | 2.2 | 1.1 | 2.7 | 1.6 | 3.5 | 4.4 | 3.9 | 3.4 | 2.8 | 1.0 | 1.2 | 3.7 | 6.3 | 6.6 | 3.6 |
Endoplasmic Reticulum | -4.0 | -4.9 | -6.6 | -8.6 | -9.1 | -10.2 | -8.7 | -9.0 | -9.5 | -3.3 | -2.2 | -7.1 | -14.4 | -13.8 | -13.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 |
Mitochondria | 3.6 | 0 | 5.8 | 6.8 | 5.8 | 8.0 | 6.7 | 8.5 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | -0.1 | 0 | 0 | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 5.8 | 5.3 | 5.7 | 6.0 | 4.4 | 4.5 | 2.0 | 5.0 | 2.9 | 3.3 | 5.5 | 5.1 | 3.4 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.9882 | 8.4769 | 7.5324 | 7.3251 | 6.2597 | 7.5881 | 8.5175 | 9.5195 | 8.8169 | 8.1795 | 7.7306 | 8.8093 | 10.5819 | 9.7686 | 9.7147 | 10.0752 | 9.4133 | 9.5218 |
Actin | 0.0052 | 0.001 | 0.0175 | 0.0055 | 0.0019 | 0.0087 | 0.0152 | 0.0004 | 0.006 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0007 | 0.0011 | 0.0074 | 0.0005 | 0.0005 |
Bud | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0.0004 |
Bud Neck | 0.0102 | 0.0007 | 0.0005 | 0.0004 | 0.0023 | 0.002 | 0.0011 | 0.0006 | 0.0018 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0028 | 0.0013 |
Bud Periphery | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0023 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0005 |
Bud Site | 0.0045 | 0.0032 | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0 | 0 | 0 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0 | 0.0001 |
Cell Periphery | 0.0024 | 0.0063 | 0.0025 | 0.0011 | 0.0036 | 0.001 | 0.0011 | 0.0064 | 0.0028 | 0.0005 | 0.0019 | 0.001 | 0.0018 | 0.0061 | 0.0034 | 0.0009 | 0.002 | 0.0019 |
Cytoplasm | 0.0415 | 0.0963 | 0.0499 | 0.0152 | 0.0101 | 0.0408 | 0.0064 | 0.0171 | 0.0067 | 0.0013 | 0.0019 | 0.0038 | 0.0114 | 0.0311 | 0.0162 | 0.0112 | 0.0969 | 0.0202 |
Cytoplasmic Foci | 0.0147 | 0.0023 | 0.0046 | 0.0013 | 0.0022 | 0.0013 | 0.0095 | 0.0005 | 0.004 | 0.0001 | 0.0001 | 0.0002 | 0.0019 | 0.0008 | 0.0004 | 0.0018 | 0.001 | 0.0005 |
Eisosomes | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.7633 | 0.8494 | 0.8468 | 0.8937 | 0.8659 | 0.9228 | 0.8856 | 0.9469 | 0.9463 | 0.9504 | 0.9491 | 0.9806 | 0.9545 | 0.9381 | 0.9612 | 0.9567 | 0.8849 | 0.9589 |
Endosome | 0.0412 | 0.0078 | 0.02 | 0.0137 | 0.0477 | 0.0051 | 0.0101 | 0.0023 | 0.0059 | 0.0003 | 0.0037 | 0.0032 | 0.0023 | 0.0022 | 0.0029 | 0.0008 | 0.0024 | 0.0019 |
Golgi | 0.008 | 0.0018 | 0.0054 | 0.0022 | 0.0095 | 0.0021 | 0.0034 | 0.0005 | 0.0033 | 0 | 0.0009 | 0.0006 | 0.0006 | 0.001 | 0.0007 | 0.0006 | 0.0003 | 0.0003 |
Lipid Particles | 0.0229 | 0.0057 | 0.0056 | 0.0025 | 0.0039 | 0.0015 | 0.0136 | 0.0081 | 0.004 | 0.0002 | 0.0025 | 0.0017 | 0.0035 | 0.0086 | 0.0029 | 0.0021 | 0.0006 | 0.0015 |
Mitochondria | 0.0021 | 0.0005 | 0.0061 | 0.0017 | 0.0099 | 0.0006 | 0.0029 | 0.0001 | 0.0086 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0003 |
None | 0.004 | 0.0005 | 0.0053 | 0.0011 | 0.0003 | 0.0006 | 0.0027 | 0.0001 | 0.0002 | 0.0274 | 0.0001 | 0.0001 | 0.0013 | 0.0004 | 0.0001 | 0.0026 | 0.0005 | 0.0002 |
Nuclear Periphery | 0.0333 | 0.0126 | 0.0184 | 0.047 | 0.0143 | 0.0079 | 0.0166 | 0.0119 | 0.004 | 0.0032 | 0.0366 | 0.0053 | 0.017 | 0.0054 | 0.0055 | 0.0079 | 0.0054 | 0.0054 |
Nucleolus | 0.0013 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0.0027 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0002 |
Nucleus | 0.0122 | 0.004 | 0.0066 | 0.0053 | 0.0021 | 0.0015 | 0.0063 | 0.001 | 0.0012 | 0.0075 | 0.0002 | 0.0003 | 0.0009 | 0.0005 | 0.0014 | 0.0013 | 0.0004 | 0.0028 |
Peroxisomes | 0.0021 | 0 | 0.001 | 0.0004 | 0.0001 | 0.0004 | 0.0063 | 0 | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0159 | 0.0007 | 0.0014 | 0.003 | 0.0002 | 0.0005 | 0.0102 | 0.0002 | 0.0003 | 0.0047 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0014 | 0.0001 | 0.0008 |
Vacuole | 0.0118 | 0.0062 | 0.0048 | 0.0034 | 0.0192 | 0.0024 | 0.0057 | 0.0033 | 0.0015 | 0.0012 | 0.0017 | 0.0013 | 0.0025 | 0.0034 | 0.0032 | 0.0013 | 0.0018 | 0.0022 |
Vacuole Periphery | 0.0024 | 0.0005 | 0.0015 | 0.0016 | 0.0035 | 0.0003 | 0.0016 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 247.1368 | 261.88 | 289.3492 | 230.7263 | 212.5003 | 261.8329 | 243.4987 | 231.8547 | 261.5729 | 223.4663 |
Translational Efficiency | 1.0485 | 0.8719 | 0.8476 | 1.0194 | 1.0849 | 0.8268 | 0.8386 | 0.845 | 0.7921 | 0.8144 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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