Standard name
Human Ortholog
Description Cytochrome b reductase; not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.1 0.07 0.05 0 0.06 0.06 0 0.06 0.09 0.05 0 0 0.12 0.11 0.18 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0.14 0.11 0.14 0 0.06 0.08 0.07 0.06 0.09 0.13 0.21 0.23 0.31 0.34 0.43 0 0 0 0 0 0
Cytoplasm 0.14 0.13 0.09 0.18 0.13 0.19 0.15 0.23 0.27 0.25 0.23 0.2 0.13 0.13 0.24 0.41 0.44 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0.75 0.85 0.87 0.68 0.65 0.56 0.45 0.42 0.36 0.44 0.4 0.39 0.73 0.78 0.52 0 0 0 0.92 0.87 0.86 0.83 0.85 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.06 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.08 0.05 0.19 0.24 0.19 0.32 0.24 0.36 0.24 0 0 0 0 0 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.22 0.19 0.21 0.23 0.14 0.15 0.05 0.17 0.08 0.09 0.2 0.19 0.1 0.05 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 7 10 16 15 13 11 15 15 8 11 22 10 3 5 15 13 19 2 3 3 1 2 4
Bud 3 1 0 1 0 2 15 6 8 5 9 6 3 0 0 1 0 4 0 0 1 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Cell Periphery 3 6 10 24 22 35 10 17 22 18 12 22 28 38 53 39 40 46 1 1 3 3 4 5
Cytoplasm 22 27 15 30 27 49 39 61 71 63 44 49 27 24 56 51 51 28 0 7 3 4 4 3
Endoplasmic Reticulum 117 173 139 115 135 141 121 114 94 110 77 98 153 139 123 1 3 3 166 192 171 120 173 156
Endosome 3 0 0 1 0 0 2 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 1 1
Golgi 0 0 1 1 1 1 0 0 0 0 0 1 0 0 0 12 9 7 0 1 1 1 1 1
Mitochondria 15 2 0 13 11 48 65 51 84 61 70 61 5 0 1 4 5 9 2 3 6 5 3 6
Nucleus 0 1 3 1 1 2 5 5 3 4 3 2 3 4 4 0 2 0 0 0 0 0 0 0
Nuclear Periphery 3 4 7 0 2 4 11 2 3 5 5 4 3 0 1 0 0 0 1 2 3 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 10 3 2 6 45 47 55 61 37 37 10 42 17 17 47 23 12 5 2 6 2 4 7 10
Unique Cell Count 156 203 160 168 209 254 268 271 262 252 193 250 211 179 235 124 116 108 180 222 200 145 204 194
Labelled Cell Count 183 224 187 208 260 342 334 333 338 311 241 307 249 225 291 147 137 123 180 222 200 145 204 194


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 12.8 9.3 9.3 9.4 8.3 7.6 8.1 7.1 7.2 6.9 7.5 10.6 10.8 10.6 13.6 15.0 14.5 11.4 11.6 11.8
Std Deviation (1e-4) 1.4 1.9 1.2 1.3 1.3 1.4 1.1 1.4 1.2 1.3 1.2 1.2 1.5 1.5 1.4 2.5 3.3 2.7 1.7 1.8 1.7
Intensity Change (Log2) 0.0 0.01 -0.16 -0.29 -0.19 -0.39 -0.36 -0.43 -0.32 0.2 0.22 0.19 0.55 0.69 0.64 0.29 0.32 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.1 0.4 -0.5 -1.0 -0.3 -0.2 -1.5 -0.2 0.9 -0.6 -2.2 -2.1 1.7 1.5 2.9
Bud 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.4 1.4 2.4 -1.2 0 0.8 0.4 0 0.9 2.2 3.9 4.3 5.6 6.0 7.2
Cytoplasm 2.2 1.1 2.7 1.6 3.5 4.4 3.9 3.4 2.8 1.0 1.2 3.7 6.3 6.6 3.6
Endoplasmic Reticulum -4.0 -4.9 -6.6 -8.6 -9.1 -10.2 -8.7 -9.0 -9.5 -3.3 -2.2 -7.1 -14.4 -13.8 -13.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0
Mitochondria 3.6 0 5.8 6.8 5.8 8.0 6.7 8.5 6.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 -0.1 0 0 0 -0.9 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.8 5.3 5.7 6.0 4.4 4.5 2.0 5.0 2.9 3.3 5.5 5.1 3.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9882 8.4769 7.5324 7.3251 6.2597 7.5881 8.5175 9.5195 8.8169 8.1795 7.7306 8.8093 10.5819 9.7686 9.7147 10.0752 9.4133 9.5218
Actin 0.0052 0.001 0.0175 0.0055 0.0019 0.0087 0.0152 0.0004 0.006 0.0001 0.0001 0.0002 0.0009 0.0007 0.0011 0.0074 0.0005 0.0005
Bud 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0 0 0.0007 0 0.0004
Bud Neck 0.0102 0.0007 0.0005 0.0004 0.0023 0.002 0.0011 0.0006 0.0018 0.0001 0.0002 0.0001 0.0001 0.0003 0.0003 0.0002 0.0028 0.0013
Bud Periphery 0.0005 0.0002 0.0005 0.0003 0.0023 0.0002 0.0005 0.0001 0.0002 0 0 0.0001 0.0002 0.0001 0.0001 0.0008 0.0001 0.0005
Bud Site 0.0045 0.0032 0.0008 0.0002 0.0004 0.0002 0.0006 0.0003 0.0005 0 0 0 0.0002 0.0007 0.0002 0.0004 0 0.0001
Cell Periphery 0.0024 0.0063 0.0025 0.0011 0.0036 0.001 0.0011 0.0064 0.0028 0.0005 0.0019 0.001 0.0018 0.0061 0.0034 0.0009 0.002 0.0019
Cytoplasm 0.0415 0.0963 0.0499 0.0152 0.0101 0.0408 0.0064 0.0171 0.0067 0.0013 0.0019 0.0038 0.0114 0.0311 0.0162 0.0112 0.0969 0.0202
Cytoplasmic Foci 0.0147 0.0023 0.0046 0.0013 0.0022 0.0013 0.0095 0.0005 0.004 0.0001 0.0001 0.0002 0.0019 0.0008 0.0004 0.0018 0.001 0.0005
Eisosomes 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001 0 0 0.0001 0.0001 0 0.0003 0 0
Endoplasmic Reticulum 0.7633 0.8494 0.8468 0.8937 0.8659 0.9228 0.8856 0.9469 0.9463 0.9504 0.9491 0.9806 0.9545 0.9381 0.9612 0.9567 0.8849 0.9589
Endosome 0.0412 0.0078 0.02 0.0137 0.0477 0.0051 0.0101 0.0023 0.0059 0.0003 0.0037 0.0032 0.0023 0.0022 0.0029 0.0008 0.0024 0.0019
Golgi 0.008 0.0018 0.0054 0.0022 0.0095 0.0021 0.0034 0.0005 0.0033 0 0.0009 0.0006 0.0006 0.001 0.0007 0.0006 0.0003 0.0003
Lipid Particles 0.0229 0.0057 0.0056 0.0025 0.0039 0.0015 0.0136 0.0081 0.004 0.0002 0.0025 0.0017 0.0035 0.0086 0.0029 0.0021 0.0006 0.0015
Mitochondria 0.0021 0.0005 0.0061 0.0017 0.0099 0.0006 0.0029 0.0001 0.0086 0 0.0001 0.0007 0.0001 0.0002 0.0001 0.0005 0.0001 0.0003
None 0.004 0.0005 0.0053 0.0011 0.0003 0.0006 0.0027 0.0001 0.0002 0.0274 0.0001 0.0001 0.0013 0.0004 0.0001 0.0026 0.0005 0.0002
Nuclear Periphery 0.0333 0.0126 0.0184 0.047 0.0143 0.0079 0.0166 0.0119 0.004 0.0032 0.0366 0.0053 0.017 0.0054 0.0055 0.0079 0.0054 0.0054
Nucleolus 0.0013 0.0001 0.0002 0.0002 0.0001 0 0.0005 0 0.0002 0.0027 0 0 0.0001 0 0 0.0003 0 0.0002
Nucleus 0.0122 0.004 0.0066 0.0053 0.0021 0.0015 0.0063 0.001 0.0012 0.0075 0.0002 0.0003 0.0009 0.0005 0.0014 0.0013 0.0004 0.0028
Peroxisomes 0.0021 0 0.001 0.0004 0.0001 0.0004 0.0063 0 0.0013 0 0 0 0 0 0 0.0001 0 0
Punctate Nuclear 0.0159 0.0007 0.0014 0.003 0.0002 0.0005 0.0102 0.0002 0.0003 0.0047 0.0002 0.0001 0.0002 0.0002 0.0002 0.0014 0.0001 0.0008
Vacuole 0.0118 0.0062 0.0048 0.0034 0.0192 0.0024 0.0057 0.0033 0.0015 0.0012 0.0017 0.0013 0.0025 0.0034 0.0032 0.0013 0.0018 0.0022
Vacuole Periphery 0.0024 0.0005 0.0015 0.0016 0.0035 0.0003 0.0016 0.0002 0.0004 0.0001 0.0007 0.0004 0.0004 0.0002 0.0002 0.0007 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 247.1368 261.88 289.3492 230.7263 212.5003 261.8329 243.4987 231.8547 261.5729 223.4663
Translational Efficiency 1.0485 0.8719 0.8476 1.0194 1.0849 0.8268 0.8386 0.845 0.7921 0.8144

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cytochrome b reductase; not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Cbr1

Cbr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available