Standard name
Human Ortholog
Description ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.07 0.12 0.09 0.11 0 0 0.08 0 0 0 0 0.07 0.06 0 0.08 0.12 0.09 0.09 0 0.1 0.05 0 0.05
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.61 0.58 0.29 0.21 0.27 0.13 0.33 0.11 0.05 0.09 0.08 0.57 0.66 0.52 0.54 0.44 0.45 0.16 0.29 0.28 0.27 0.16 0.15
Endoplasmic Reticulum – 0.15 0.12 0.16 0.21 0.18 0.07 0.12 0.07 0 0.05 0.09 0.33 0.16 0.1 0.06 0.05 0.05 0.06 0 0 0 0 0
Endosome – 0 0.12 0.07 0.12 0.13 0.17 0.07 0.05 0.05 0.1 0 0.07 0.11 0.19 0.21 0.18 0.15 0.28 0.24 0.26 0.16 0.26 0.32
Golgi – 0 0 0.22 0.28 0.09 0.09 0.05 0.05 0 0 0 0 0 0 0.07 0.2 0.1 0.32 0.06 0.15 0.15 0.19 0.23
Mitochondria – 0.12 0 0.22 0 0.29 0.37 0.26 0.72 0.76 0.66 0.69 0 0 0 0.05 0.07 0 0 0.13 0.07 0.09 0.1 0.08
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.08 0 0.07 0.18 0.2 0.21 0.17 0.09 0.1 0.18 0.1 0.05 0.07 0.19 0.14 0.18 0.3 0 0.15 0 0.1 0.09 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 19 3 4 11 2 2 8 7 8 3 7 13 15 10 23 29 18 2 12 6 9 13 16
Bud – 13 0 0 0 0 3 2 7 3 6 6 0 0 3 3 1 2 0 7 0 5 7 3
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 3 4 1
Bud Site – 0 0 0 0 0 1 1 1 3 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 0 0 0 0 0 1 1 3 2 1 0 2 3 2 2 6 0 0 0 0 0 0
Cytoplasm – 177 14 13 21 12 16 33 22 9 15 15 111 176 136 161 109 96 4 121 18 51 53 46
Endoplasmic Reticulum – 43 3 7 21 8 9 12 15 6 8 18 64 44 27 18 13 11 1 9 1 3 14 9
Endosome – 2 3 3 12 6 20 7 10 10 18 7 13 29 50 63 44 31 7 96 16 29 88 97
Golgi – 1 0 10 28 4 11 5 10 7 1 5 4 6 8 21 50 21 9 25 10 28 65 69
Mitochondria – 35 0 10 2 13 44 26 144 147 114 133 1 2 3 15 16 5 0 53 4 16 35 24
Nucleus – 0 1 0 0 1 1 4 1 1 1 4 0 1 0 0 1 3 0 3 0 2 3 3
Nuclear Periphery – 0 0 0 0 0 0 0 0 1 2 3 0 0 0 0 0 0 1 1 1 2 3 0
Nucleolus – 1 0 0 0 0 4 0 3 3 7 9 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole – 1 0 0 1 0 1 3 2 1 4 1 1 1 4 4 4 0 0 6 1 5 10 4
Vac/Vac Membrane – 22 1 3 18 9 25 17 18 20 31 19 10 19 49 43 45 64 0 61 1 18 30 18
Unique Cell Count 290 24 45 101 45 120 100 200 193 173 193 196 268 261 299 246 211 29 412 66 186 343 306
Labelled Cell Count 315 25 50 114 55 138 119 241 222 212 228 217 296 293 354 314 257 29 412 66 186 343 306


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 6.9 10.1 9.4 10.1 8.7 7.4 7.8 7.0 6.6 6.9 6.8 9.9 9.9 9.4 9.4 11.8 12.3 10.4 10.3 10.2
Std Deviation (1e-4) – 1.2 1.0 1.3 1.4 1.7 1.4 1.7 1.5 1.1 1.3 1.2 1.4 1.3 1.3 1.8 2.2 2.8 1.4 1.4 1.7
Intensity Change (Log2) – – – -0.11 0.0 -0.22 -0.46 -0.37 -0.54 -0.62 -0.55 -0.58 -0.03 -0.03 -0.11 -0.1 0.22 0.28 0.03 0.02 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.4 -3.7 -2.6 -5.0 -2.3 0 0 0 0 -0.2 0.7 -0.6 -0.4 -1.3 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 2.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.6 2.8 7.0 7.5 6.1 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4294 9.5308 8.7955 8.0609 7.8327 8.6897 7.3741 9.6301 8.1583 7.8094 7.9231 8.0485 5.2535 7.2202 6.4034 6.2649 5.5551 6.6186
Actin 0.0966 0.0407 0.0299 0.0021 0.0219 0.0112 0.0139 0.0213 0.0289 0.007 0.0113 0.0172 0.0588 0.0059 0.0169 0.0101 0.0076 0.0102
Bud 0.0016 0.0024 0.0035 0.0008 0.0015 0.0008 0.001 0.0011 0.0015 0.0003 0.0009 0.0005 0.0008 0.0013 0.0016 0.001 0.0007 0.0008
Bud Neck 0.0029 0.0012 0.0012 0.003 0.001 0.0122 0.0028 0.0008 0.002 0.0013 0.0009 0.0032 0.0029 0.0008 0.0018 0.0029 0.0012 0.0072
Bud Periphery 0.0007 0.0006 0.001 0.0004 0.0006 0.0003 0.0012 0.0003 0.0011 0.0001 0.0004 0.0002 0.0007 0.0003 0.0004 0.0003 0.0003 0.0003
Bud Site 0.0103 0.0133 0.0129 0.0016 0.0012 0.0011 0.0082 0.0068 0.0122 0.0003 0.0012 0.0008 0.0031 0.0105 0.0032 0.0011 0.0011 0.001
Cell Periphery 0.0008 0.0002 0.0005 0.0002 0.0001 0.0001 0.0021 0.0002 0.0009 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.2777 0.4314 0.3475 0.3394 0.2231 0.2371 0.2228 0.4578 0.3092 0.3286 0.3594 0.3198 0.2406 0.5125 0.3562 0.3162 0.3069 0.3186
Cytoplasmic Foci 0.1838 0.1708 0.2018 0.1517 0.24 0.2446 0.2392 0.2201 0.2331 0.2309 0.1621 0.2245 0.2166 0.2006 0.2008 0.2084 0.1986 0.2428
Eisosomes 0.0002 0 0.0001 0 0 0 0.0014 0.0001 0.0001 0 0 0 0.0005 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.016 0.0091 0.0045 0.0068 0.009 0.01 0.0099 0.0157 0.005 0.0106 0.0025 0.0067 0.0171 0.0077 0.0061 0.0117 0.0096 0.0069
Endosome 0.2244 0.2199 0.2401 0.3845 0.3016 0.2948 0.2385 0.1358 0.2699 0.2783 0.328 0.2758 0.2733 0.1653 0.2731 0.338 0.292 0.2732
Golgi 0.0729 0.0891 0.1137 0.0573 0.1134 0.1457 0.0906 0.0666 0.0698 0.0779 0.097 0.0972 0.0911 0.0549 0.0891 0.0638 0.0729 0.0946
Lipid Particles 0.0251 0.001 0.0017 0.0033 0.0055 0.0018 0.0104 0.0011 0.0039 0.0007 0.002 0.0019 0.0258 0.0007 0.0022 0.0044 0.0093 0.0023
Mitochondria 0.0064 0.0046 0.0086 0.0223 0.0157 0.0091 0.0092 0.0038 0.0061 0.0028 0.0089 0.0139 0.0105 0.0037 0.0151 0.0072 0.0206 0.0077
None 0.0114 0.0013 0.0034 0.0026 0.0011 0.0008 0.0429 0.0205 0.0018 0.0383 0.0008 0.0012 0.0078 0.0014 0.0025 0.0042 0.0038 0.0012
Nuclear Periphery 0.0095 0.0014 0.0009 0.0022 0.0035 0.0016 0.0485 0.0008 0.0022 0.0015 0.001 0.0014 0.0064 0.0026 0.0018 0.0087 0.0164 0.0066
Nucleolus 0.0003 0.0001 0.0004 0.0001 0.0001 0 0.0037 0.0001 0.0164 0 0 0.0151 0.0005 0.0001 0.0001 0.0001 0.0003 0.0001
Nucleus 0.0056 0.0011 0.0008 0.0013 0.0037 0.0011 0.026 0.001 0.0024 0.0006 0.0011 0.0018 0.002 0.0023 0.001 0.0012 0.002 0.0026
Peroxisomes 0.0367 0.005 0.0222 0.0036 0.0492 0.0219 0.0123 0.0417 0.0255 0.0165 0.0063 0.0125 0.029 0.0181 0.0208 0.0082 0.0432 0.0156
Punctate Nuclear 0.0086 0.0006 0.0007 0.0009 0.0013 0.0006 0.0037 0.0012 0.0009 0.0004 0.0004 0.001 0.0052 0.001 0.0018 0.0026 0.0016 0.0029
Vacuole 0.0065 0.0045 0.0028 0.0111 0.0035 0.0031 0.0054 0.0023 0.0048 0.0024 0.0117 0.0031 0.0045 0.0085 0.0032 0.0067 0.0061 0.0035
Vacuole Periphery 0.0019 0.0016 0.0018 0.005 0.0028 0.0019 0.0065 0.001 0.0023 0.0014 0.0042 0.0022 0.0027 0.0016 0.0021 0.0032 0.0055 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.5978 26.3913 22.0314 38.5142 36.6824 29.5062 27.5181 34.5193 33.8832 40.0893
Translational Efficiency 1.6178 1.6526 1.8152 0.8667 1.2515 1.9302 1.7385 1.2524 1.3855 1.1487

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-3

Age2

Age2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Age2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available