Standard name | |
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Human Ortholog | |
Description | Putative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 0 | 0 |
Bud | 1 | 2 | 3 | 1 | 3 | 3 | 2 | 4 | 5 | 0 | 3 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 1 | 4 | 1 | 6 | 1 | 6 | 5 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 1 | 2 | 2 | 1 | 2 | 5 | 2 | 1 | 3 | 2 | 2 | 6 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 59 | 262 | 213 | 170 | 270 | 220 | 199 | 229 | 206 | 210 | 90 | 111 | 280 | 256 | 313 | 77 | 86 | 46 | 159 | 251 | 153 | 173 | 288 | 162 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 2 | 1 | 1 | 4 | 4 | 2 | 10 | 0 | 1 | 2 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 2 | 0 | 6 | 2 | 3 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 13 | 7 | 10 | 15 | 18 | 56 | 72 | 48 | 78 | 76 | 46 | 46 | 0 | 0 | 0 | 5 | 5 | 0 | 27 | 3 | 16 | 2 | 6 | 2 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 5 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 2 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 0 | 7 | 3 | 1 | 0 | 0 | 4 |
Unique Cell Count | 72 | 267 | 218 | 173 | 277 | 247 | 232 | 259 | 252 | 254 | 115 | 136 | 282 | 260 | 314 | 84 | 96 | 47 | 233 | 269 | 195 | 184 | 311 | 180 |
Labelled Cell Count | 77 | 273 | 234 | 192 | 300 | 289 | 286 | 297 | 306 | 297 | 144 | 165 | 285 | 264 | 321 | 95 | 107 | 53 | 233 | 269 | 195 | 184 | 311 | 180 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.0 | 3.2 | 3.1 | 3.2 | 2.8 | 2.4 | 3.4 | 2.8 | 2.6 | 2.8 | 2.7 | 4.5 | 4.6 | 4.5 | 4.9 | 5.7 | 5.1 | 3.9 | 3.8 | 5.0 |
Std Deviation (1e-4) | 1.1 | 0.8 | 0.6 | 1.1 | 1.0 | 0.9 | 0.9 | 5.3 | 1.1 | 1.0 | 1.3 | 1.3 | 1.3 | 1.6 | 1.5 | 1.9 | 1.8 | 1.3 | 1.4 | 1.0 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.0 | -0.16 | -0.38 | 0.09 | -0.17 | -0.3 | -0.17 | -0.25 | 0.51 | 0.54 | 0.51 | 0.63 | 0.85 | 0.7 | 0.3 | 0.26 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6238 | 2.2772 | 2.7396 | 1.9809 | 3.2487 | 2.5586 | 2.9525 | 1.1555 | 1.6268 | 0.5863 | 1.8661 | 1.1567 | 1.0664 | 2.277 | 1.4671 | 2.0321 | 2.3659 | 1.728 |
Actin | 0.0308 | 0.0001 | 0.0121 | 0.0095 | 0.0161 | 0.0207 | 0.2201 | 0.0001 | 0.043 | 0 | 0.0002 | 0.0015 | 0.0264 | 0.0047 | 0.0058 | 0.0117 | 0 | 0.0369 |
Bud | 0.0005 | 0 | 0.0069 | 0.0063 | 0.0003 | 0.0002 | 0.0011 | 0 | 0.0002 | 0 | 0 | 0 | 0.0116 | 0.0004 | 0.007 | 0.0003 | 0 | 0.0015 |
Bud Neck | 0.0026 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0036 | 0 | 0.0016 | 0 | 0 | 0.0006 | 0.0122 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0043 |
Bud Periphery | 0.0009 | 0 | 0.0097 | 0.0114 | 0.0009 | 0.0004 | 0.0014 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0294 | 0.0003 | 0.0141 | 0.0004 | 0 | 0.0064 |
Bud Site | 0.0087 | 0 | 0.0305 | 0.0011 | 0.0004 | 0.0001 | 0.0197 | 0 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0066 | 0.0043 | 0.0015 | 0.0023 | 0 | 0.0006 |
Cell Periphery | 0.0006 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0005 |
Cytoplasm | 0.0107 | 0.1338 | 0.0872 | 0.0834 | 0.0103 | 0.1772 | 0.0201 | 0.0666 | 0.0338 | 0.018 | 0.0103 | 0.058 | 0.0843 | 0.0774 | 0.1201 | 0.0358 | 0.0156 | 0.1016 |
Cytoplasmic Foci | 0.0331 | 0.0015 | 0.0213 | 0.0217 | 0.0026 | 0.0015 | 0.0539 | 0.0012 | 0.0501 | 0.0006 | 0.0264 | 0.0124 | 0.0044 | 0.0064 | 0.0038 | 0.0067 | 0.0005 | 0.0097 |
Eisosomes | 0.0028 | 0.0001 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0 | 0.0003 |
Endoplasmic Reticulum | 0.0018 | 0.0016 | 0.0045 | 0.0019 | 0.0015 | 0.0006 | 0.0115 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0008 | 0.0018 | 0.0029 | 0.0013 | 0.0013 | 0.0002 | 0.003 |
Endosome | 0.0171 | 0.0005 | 0.0192 | 0.0019 | 0.0193 | 0.0003 | 0.1022 | 0.0002 | 0.006 | 0 | 0.0781 | 0.0083 | 0.0104 | 0.0187 | 0.0035 | 0.026 | 0 | 0.0051 |
Golgi | 0.0061 | 0.0001 | 0.0118 | 0.0006 | 0.0248 | 0.0001 | 0.0319 | 0 | 0.0141 | 0 | 0.0149 | 0.0017 | 0.0158 | 0.0053 | 0.0008 | 0.0168 | 0 | 0.007 |
Lipid Particles | 0.0547 | 0.0002 | 0.0083 | 0.0011 | 0.0421 | 0 | 0.0745 | 0.0001 | 0.0107 | 0.0001 | 0.0675 | 0.001 | 0.0157 | 0.0006 | 0.0051 | 0.0065 | 0 | 0.0026 |
Mitochondria | 0.0045 | 0.0001 | 0.012 | 0.0018 | 0.1237 | 0.0001 | 0.0147 | 0.0001 | 0.0021 | 0.0001 | 0.0019 | 0.0003 | 0.0047 | 0.0034 | 0.0024 | 0.0135 | 0 | 0.0016 |
None | 0.7729 | 0.861 | 0.7573 | 0.8482 | 0.7399 | 0.7981 | 0.3366 | 0.9303 | 0.7649 | 0.9804 | 0.7891 | 0.9063 | 0.7628 | 0.8677 | 0.8258 | 0.873 | 0.9833 | 0.8015 |
Nuclear Periphery | 0.0018 | 0.0002 | 0.0018 | 0.0019 | 0.0061 | 0.0001 | 0.0104 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0011 | 0.003 | 0.0022 | 0.0043 | 0.0008 | 0 | 0.0037 |
Nucleolus | 0.0034 | 0 | 0.0025 | 0.0004 | 0.0003 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0003 |
Nucleus | 0.0012 | 0.0002 | 0.0018 | 0.001 | 0.0006 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0006 | 0.0006 | 0.0006 | 0.0004 | 0 | 0.0013 |
Peroxisomes | 0.0335 | 0 | 0.0026 | 0.0018 | 0.0052 | 0.0001 | 0.0813 | 0 | 0.0693 | 0 | 0.0075 | 0.0008 | 0.0045 | 0.0005 | 0.0008 | 0.0011 | 0 | 0.0057 |
Punctate Nuclear | 0.0102 | 0.0001 | 0.0032 | 0.0038 | 0.0009 | 0.0001 | 0.0044 | 0.0001 | 0.0014 | 0 | 0.0008 | 0.006 | 0.0014 | 0.0003 | 0.0012 | 0.0006 | 0 | 0.005 |
Vacuole | 0.0017 | 0.0003 | 0.0031 | 0.0011 | 0.001 | 0.0001 | 0.0044 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0022 | 0.002 | 0.0005 | 0.0012 | 0 | 0.0011 |
Vacuole Periphery | 0.0006 | 0 | 0.0024 | 0.0004 | 0.0032 | 0 | 0.0026 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.001 | 0.0009 | 0.0006 | 0.0007 | 0 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.1414 | 2.44 | 1.8106 | 4.1994 | 3.0966 | 4.092 | 2.6213 | 4.9954 | 5.3237 | 4.7888 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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