Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 22 | 12 | 12 | 34 | 26 | 26 | 35 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 5 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 10 | 10 | 24 | 32 | 19 | 22 | 39 | 31 | 28 | 35 | 0 | 2 | 11 | 2 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 127 | 116 | 449 | 312 | 420 | 342 | 376 | 306 | 297 | 294 | 228 | 226 | 150 | 308 | 390 | 100 | 104 | 131 | 124 | 108 | 408 | 160 | 362 | 395 |
Endoplasmic Reticulum | 3 | 7 | 29 | 18 | 80 | 62 | 134 | 121 | 83 | 100 | 72 | 87 | 5 | 13 | 2 | 1 | 5 | 5 | 1 | 2 | 34 | 6 | 12 | 15 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Golgi | 0 | 0 | 2 | 0 | 21 | 19 | 44 | 47 | 46 | 36 | 20 | 46 | 1 | 0 | 4 | 2 | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 5 | 9 | 3 | 41 | 23 | 39 | 33 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 3 | 3 | 23 | 42 | 14 | 6 | 4 | 8 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 4 | 3 | 2 | 7 | 4 |
Unique Cell Count | 133 | 123 | 474 | 326 | 510 | 439 | 556 | 505 | 460 | 456 | 362 | 399 | 155 | 320 | 394 | 102 | 106 | 136 | 131 | 119 | 465 | 176 | 398 | 432 |
Labelled Cell Count | 133 | 126 | 490 | 341 | 551 | 486 | 618 | 563 | 560 | 519 | 418 | 473 | 158 | 325 | 409 | 107 | 110 | 145 | 131 | 119 | 465 | 176 | 398 | 432 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 64.7 | 56.9 | 55.9 | 50.8 | 41.1 | 31.7 | 28.9 | 25.2 | 23.1 | 22.4 | 21.4 | 22.1 | 50.8 | 49.5 | 47.5 | 93.8 | 115.8 | 131.8 | 50.9 | 62.2 | 61.3 |
Std Deviation (1e-4) | 16.2 | 12.7 | 12.9 | 12.5 | 9.9 | 8.0 | 8.2 | 6.9 | 7.0 | 5.4 | 5.5 | 5.9 | 13.5 | 12.0 | 11.9 | 27.7 | 34.1 | 41.1 | 17.1 | 21.5 | 19.1 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.44 | -0.82 | -0.95 | -1.15 | -1.27 | -1.32 | -1.39 | -1.34 | -0.14 | -0.18 | -0.24 | 0.75 | 1.05 | 1.24 | -0.14 | 0.15 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 4.9 | 3.2 | 3.4 | 6.0 | 5.3 | 5.9 | 6.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | 2.2 | 3.7 | 1.3 | 2.0 | 4.4 | 3.5 | 3.9 | 4.4 | 0 | 0 | 0.7 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -6.0 | -7.5 | -10.9 | -12.7 | -11.5 | -11.5 | -11.6 | -13.4 | 1.0 | 1.0 | 3.5 | 1.4 | 1.5 | 0.8 |
Endoplasmic Reticulum | -0.4 | 4.8 | 4.0 | 7.9 | 7.7 | 5.6 | 7.0 | 6.1 | 6.8 | -1.4 | -1.3 | -4.4 | -2.1 | -0.6 | -1.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 3.8 | 3.9 | 5.8 | 6.4 | 6.6 | 5.8 | 4.6 | 7.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 6.6 | 5.0 | 7.3 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 4.5 | 6.4 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 87.0305 | 102.7082 | 94.9639 | 92.0529 | 93.8321 | 91.557 | 78.0292 | 101.6711 | 84.2723 | 91.2334 | 77.9023 | 102.6889 | 98.1105 | 113.1429 | 104.4932 | 99.5415 | 101.193 | 109.6911 |
Actin | 0.0144 | 0.003 | 0.0016 | 0.0013 | 0.0082 | 0.0066 | 0.0232 | 0.0008 | 0.0091 | 0.0005 | 0.0095 | 0.0082 | 0.004 | 0.0002 | 0.0007 | 0.0014 | 0.0004 | 0.0008 |
Bud | 0.0032 | 0.0006 | 0.0036 | 0.003 | 0.0024 | 0.0128 | 0.0023 | 0.0006 | 0.005 | 0.0027 | 0.0016 | 0.0056 | 0.0022 | 0.0003 | 0.0005 | 0.0008 | 0.0006 | 0.0004 |
Bud Neck | 0.0014 | 0.0006 | 0.0007 | 0.0016 | 0.0056 | 0.0025 | 0.0011 | 0.0003 | 0.001 | 0.0008 | 0.0007 | 0.0013 | 0.0023 | 0.0004 | 0.0006 | 0.0005 | 0.0005 | 0.0015 |
Bud Periphery | 0.0047 | 0.0004 | 0.0031 | 0.0034 | 0.0016 | 0.0073 | 0.0023 | 0.0003 | 0.0074 | 0.0017 | 0.0011 | 0.0028 | 0.0024 | 0.0001 | 0.0004 | 0.0005 | 0.0006 | 0.0003 |
Bud Site | 0.0014 | 0.0074 | 0.0012 | 0.0005 | 0.0008 | 0.0009 | 0.0023 | 0.0004 | 0.0037 | 0.0005 | 0.0006 | 0.0006 | 0.0042 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0002 |
Cell Periphery | 0.0224 | 0.0071 | 0.0086 | 0.0055 | 0.0087 | 0.002 | 0.0072 | 0.001 | 0.004 | 0.0077 | 0.0059 | 0.0013 | 0.0442 | 0.0065 | 0.008 | 0.0085 | 0.0006 | 0.0087 |
Cytoplasm | 0.5782 | 0.9238 | 0.8567 | 0.5865 | 0.6702 | 0.7106 | 0.6756 | 0.968 | 0.8355 | 0.7983 | 0.7674 | 0.7903 | 0.6609 | 0.9606 | 0.8642 | 0.8004 | 0.9027 | 0.866 |
Cytoplasmic Foci | 0.0027 | 0.0017 | 0.0034 | 0.0023 | 0.0206 | 0.0068 | 0.023 | 0.0016 | 0.009 | 0.0093 | 0.006 | 0.0088 | 0.0224 | 0.0007 | 0.0072 | 0.0044 | 0.0021 | 0.0035 |
Eisosomes | 0.0028 | 0 | 0.0004 | 0.0019 | 0.001 | 0 | 0.0006 | 0 | 0.0004 | 0 | 0.0006 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0.0005 |
Endoplasmic Reticulum | 0.0587 | 0.0173 | 0.0141 | 0.0224 | 0.008 | 0.0738 | 0.0265 | 0.0068 | 0.0198 | 0.002 | 0.0326 | 0.0053 | 0.0662 | 0.007 | 0.0195 | 0.0121 | 0.0084 | 0.0214 |
Endosome | 0.0242 | 0.0056 | 0.0105 | 0.019 | 0.0342 | 0.0326 | 0.0238 | 0.0033 | 0.027 | 0.0316 | 0.0155 | 0.0306 | 0.0145 | 0.0015 | 0.0076 | 0.0077 | 0.0073 | 0.0094 |
Golgi | 0.0065 | 0.0022 | 0.0013 | 0.0018 | 0.0201 | 0.0064 | 0.005 | 0.0015 | 0.0041 | 0.0033 | 0.0226 | 0.0026 | 0.0026 | 0.0002 | 0.0013 | 0.0031 | 0.0009 | 0.0006 |
Lipid Particles | 0.0031 | 0.0022 | 0.0015 | 0.0056 | 0.0102 | 0.0002 | 0.0071 | 0.0002 | 0.0011 | 0.0013 | 0.0047 | 0.0005 | 0.0041 | 0.0006 | 0.0036 | 0.009 | 0.0032 | 0.0038 |
Mitochondria | 0.0203 | 0.0014 | 0.0077 | 0.0022 | 0.0384 | 0.0156 | 0.016 | 0.0004 | 0.0125 | 0.0116 | 0.0273 | 0.0451 | 0.0107 | 0.0002 | 0.0025 | 0.0037 | 0.0444 | 0.0061 |
None | 0.0018 | 0.0002 | 0.0018 | 0.0041 | 0.0097 | 0.0008 | 0.0068 | 0.0003 | 0.0014 | 0.0039 | 0.0173 | 0.0018 | 0.0054 | 0.0001 | 0.0017 | 0.0012 | 0.0003 | 0.0016 |
Nuclear Periphery | 0.074 | 0.004 | 0.0115 | 0.0155 | 0.0042 | 0.0386 | 0.0421 | 0.0016 | 0.0057 | 0.0067 | 0.0073 | 0.0109 | 0.0439 | 0.0033 | 0.0096 | 0.0183 | 0.0046 | 0.0125 |
Nucleolus | 0.0039 | 0 | 0.0012 | 0.0007 | 0.027 | 0.0002 | 0.0086 | 0.0001 | 0.001 | 0.0012 | 0.0007 | 0.0032 | 0.0102 | 0.0001 | 0.0038 | 0.0006 | 0.001 | 0.0087 |
Nucleus | 0.0149 | 0.0015 | 0.0041 | 0.0066 | 0.0064 | 0.0087 | 0.0216 | 0.0022 | 0.005 | 0.0074 | 0.0098 | 0.0357 | 0.014 | 0.0016 | 0.0051 | 0.0061 | 0.0019 | 0.0057 |
Peroxisomes | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0019 | 0.0006 | 0.0092 | 0 | 0.0019 | 0.0003 | 0.0004 | 0.0013 | 0.0023 | 0 | 0.0037 | 0.004 | 0.0006 | 0.0003 |
Punctate Nuclear | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0015 | 0.0001 | 0.0041 | 0 | 0.0004 | 0.0002 | 0.0009 | 0.0002 | 0.0015 | 0 | 0.0002 | 0.0003 | 0 | 0.0002 |
Vacuole | 0.103 | 0.0176 | 0.0464 | 0.2147 | 0.0781 | 0.0446 | 0.0552 | 0.0088 | 0.0328 | 0.0853 | 0.05 | 0.0162 | 0.0635 | 0.0148 | 0.046 | 0.0914 | 0.0129 | 0.0274 |
Vacuole Periphery | 0.0574 | 0.0031 | 0.0201 | 0.1009 | 0.0411 | 0.0281 | 0.0364 | 0.0019 | 0.0124 | 0.0237 | 0.0175 | 0.0276 | 0.0182 | 0.0018 | 0.0135 | 0.0255 | 0.0066 | 0.0206 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1777.2047 | 1794.8812 | 1975.821 | 1823.2277 | 2054.0619 | 1557.7713 | 1510.2918 | 1426.2556 | 1622.9101 | 1455.5028 |
Translational Efficiency | 1.9058 | 1.9762 | 1.8347 | 1.8676 | 1.8769 | 1.9838 | 1.9057 | 2.1465 | 1.6651 | 1.8593 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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