Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.05 0 0 0.07 0.06 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.05 0.07 0 0 0.08 0.07 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.94 0.95 0.96 0.82 0.78 0.68 0.61 0.65 0.64 0.63 0.57 0.97 0.96 0.99 0.98 0.98 0.96 0.95 0.92 0.88 0.91 0.91 0.92
Endoplasmic Reticulum 0 0.06 0.06 0.06 0.16 0.14 0.24 0.24 0.18 0.22 0.2 0.22 0 0 0 0 0.05 0 0 0 0.08 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.08 0.09 0.1 0.08 0.06 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0.05 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 22 12 12 34 26 26 35 0 0 0 0 0 0 0 0 1 0 1 0
Bud 0 0 0 0 1 0 0 2 5 1 1 1 0 0 0 0 0 0 0 0 2 1 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 2 10 10 24 32 19 22 39 31 28 35 0 2 11 2 0 4 0 0 1 0 0 0
Cytoplasm 127 116 449 312 420 342 376 306 297 294 228 226 150 308 390 100 104 131 124 108 408 160 362 395
Endoplasmic Reticulum 3 7 29 18 80 62 134 121 83 100 72 87 5 13 2 1 5 5 1 2 34 6 12 15
Endosome 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0
Golgi 0 0 2 0 21 19 44 47 46 36 20 46 1 0 4 2 1 4 0 0 1 0 0 1
Mitochondria 0 0 0 0 1 5 9 3 41 23 39 33 0 0 1 1 0 0 0 0 0 0 1 0
Nucleus 0 1 0 0 0 1 1 3 0 2 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 0 3 3 23 42 14 6 4 8 2 1 1 0 0 0 1 4 3 2 7 4
Unique Cell Count 133 123 474 326 510 439 556 505 460 456 362 399 155 320 394 102 106 136 131 119 465 176 398 432
Labelled Cell Count 133 126 490 341 551 486 618 563 560 519 418 473 158 325 409 107 110 145 131 119 465 176 398 432


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.7 56.9 55.9 50.8 41.1 31.7 28.9 25.2 23.1 22.4 21.4 22.1 50.8 49.5 47.5 93.8 115.8 131.8 50.9 62.2 61.3
Std Deviation (1e-4) 16.2 12.7 12.9 12.5 9.9 8.0 8.2 6.9 7.0 5.4 5.5 5.9 13.5 12.0 11.9 27.7 34.1 41.1 17.1 21.5 19.1
Intensity Change (Log2) -0.14 -0.44 -0.82 -0.95 -1.15 -1.27 -1.32 -1.39 -1.34 -0.14 -0.18 -0.24 0.75 1.05 1.24 -0.14 0.15 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 4.9 3.2 3.4 6.0 5.3 5.9 6.6 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 2.2 3.7 1.3 2.0 4.4 3.5 3.9 4.4 0 0 0.7 0 0 0
Cytoplasm 0.6 -6.0 -7.5 -10.9 -12.7 -11.5 -11.5 -11.6 -13.4 1.0 1.0 3.5 1.4 1.5 0.8
Endoplasmic Reticulum -0.4 4.8 4.0 7.9 7.7 5.6 7.0 6.1 6.8 -1.4 -1.3 -4.4 -2.1 -0.6 -1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 3.8 3.9 5.8 6.4 6.6 5.8 4.6 7.2 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 6.6 5.0 7.3 6.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.5 6.4 3.8 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 87.0305 102.7082 94.9639 92.0529 93.8321 91.557 78.0292 101.6711 84.2723 91.2334 77.9023 102.6889 98.1105 113.1429 104.4932 99.5415 101.193 109.6911
Actin 0.0144 0.003 0.0016 0.0013 0.0082 0.0066 0.0232 0.0008 0.0091 0.0005 0.0095 0.0082 0.004 0.0002 0.0007 0.0014 0.0004 0.0008
Bud 0.0032 0.0006 0.0036 0.003 0.0024 0.0128 0.0023 0.0006 0.005 0.0027 0.0016 0.0056 0.0022 0.0003 0.0005 0.0008 0.0006 0.0004
Bud Neck 0.0014 0.0006 0.0007 0.0016 0.0056 0.0025 0.0011 0.0003 0.001 0.0008 0.0007 0.0013 0.0023 0.0004 0.0006 0.0005 0.0005 0.0015
Bud Periphery 0.0047 0.0004 0.0031 0.0034 0.0016 0.0073 0.0023 0.0003 0.0074 0.0017 0.0011 0.0028 0.0024 0.0001 0.0004 0.0005 0.0006 0.0003
Bud Site 0.0014 0.0074 0.0012 0.0005 0.0008 0.0009 0.0023 0.0004 0.0037 0.0005 0.0006 0.0006 0.0042 0.0002 0.0002 0.0004 0.0001 0.0002
Cell Periphery 0.0224 0.0071 0.0086 0.0055 0.0087 0.002 0.0072 0.001 0.004 0.0077 0.0059 0.0013 0.0442 0.0065 0.008 0.0085 0.0006 0.0087
Cytoplasm 0.5782 0.9238 0.8567 0.5865 0.6702 0.7106 0.6756 0.968 0.8355 0.7983 0.7674 0.7903 0.6609 0.9606 0.8642 0.8004 0.9027 0.866
Cytoplasmic Foci 0.0027 0.0017 0.0034 0.0023 0.0206 0.0068 0.023 0.0016 0.009 0.0093 0.006 0.0088 0.0224 0.0007 0.0072 0.0044 0.0021 0.0035
Eisosomes 0.0028 0 0.0004 0.0019 0.001 0 0.0006 0 0.0004 0 0.0006 0.0001 0.0003 0 0.0001 0 0.0003 0.0005
Endoplasmic Reticulum 0.0587 0.0173 0.0141 0.0224 0.008 0.0738 0.0265 0.0068 0.0198 0.002 0.0326 0.0053 0.0662 0.007 0.0195 0.0121 0.0084 0.0214
Endosome 0.0242 0.0056 0.0105 0.019 0.0342 0.0326 0.0238 0.0033 0.027 0.0316 0.0155 0.0306 0.0145 0.0015 0.0076 0.0077 0.0073 0.0094
Golgi 0.0065 0.0022 0.0013 0.0018 0.0201 0.0064 0.005 0.0015 0.0041 0.0033 0.0226 0.0026 0.0026 0.0002 0.0013 0.0031 0.0009 0.0006
Lipid Particles 0.0031 0.0022 0.0015 0.0056 0.0102 0.0002 0.0071 0.0002 0.0011 0.0013 0.0047 0.0005 0.0041 0.0006 0.0036 0.009 0.0032 0.0038
Mitochondria 0.0203 0.0014 0.0077 0.0022 0.0384 0.0156 0.016 0.0004 0.0125 0.0116 0.0273 0.0451 0.0107 0.0002 0.0025 0.0037 0.0444 0.0061
None 0.0018 0.0002 0.0018 0.0041 0.0097 0.0008 0.0068 0.0003 0.0014 0.0039 0.0173 0.0018 0.0054 0.0001 0.0017 0.0012 0.0003 0.0016
Nuclear Periphery 0.074 0.004 0.0115 0.0155 0.0042 0.0386 0.0421 0.0016 0.0057 0.0067 0.0073 0.0109 0.0439 0.0033 0.0096 0.0183 0.0046 0.0125
Nucleolus 0.0039 0 0.0012 0.0007 0.027 0.0002 0.0086 0.0001 0.001 0.0012 0.0007 0.0032 0.0102 0.0001 0.0038 0.0006 0.001 0.0087
Nucleus 0.0149 0.0015 0.0041 0.0066 0.0064 0.0087 0.0216 0.0022 0.005 0.0074 0.0098 0.0357 0.014 0.0016 0.0051 0.0061 0.0019 0.0057
Peroxisomes 0.0006 0.0001 0.0004 0.0002 0.0019 0.0006 0.0092 0 0.0019 0.0003 0.0004 0.0013 0.0023 0 0.0037 0.004 0.0006 0.0003
Punctate Nuclear 0.0004 0 0.0001 0.0003 0.0015 0.0001 0.0041 0 0.0004 0.0002 0.0009 0.0002 0.0015 0 0.0002 0.0003 0 0.0002
Vacuole 0.103 0.0176 0.0464 0.2147 0.0781 0.0446 0.0552 0.0088 0.0328 0.0853 0.05 0.0162 0.0635 0.0148 0.046 0.0914 0.0129 0.0274
Vacuole Periphery 0.0574 0.0031 0.0201 0.1009 0.0411 0.0281 0.0364 0.0019 0.0124 0.0237 0.0175 0.0276 0.0182 0.0018 0.0135 0.0255 0.0066 0.0206

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1777.2047 1794.8812 1975.821 1823.2277 2054.0619 1557.7713 1510.2918 1426.2556 1622.9101 1455.5028
Translational Efficiency 1.9058 1.9762 1.8347 1.8676 1.8769 1.9838 1.9057 2.1465 1.6651 1.8593

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl34b

Rpl34b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl34b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available