Standard name
Human Ortholog
Description Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.08 0.06 0.08 – 0.05 0.07 0 0 0.12 0.21 0.22 0.14 0.16 0.13 0 0 0 0 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.14 0.08 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.05 0.06 0.22 0.21 – 0.42 0.29 0.58 0.44 0 0 0 0 0 0 0 0 0 0.05 0 0
Nucleus 0.75 0.6 0.78 0.88 0.69 0.66 0.69 – 0.67 0.76 0.67 0.72 0.69 0.6 0.63 0.51 0.43 0.46 0.65 0.62 0.59 0.58 0.5 0.42
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.24 0.4 0.19 0.07 0.17 0.15 0.13 – 0.07 0.05 0 0 0.22 0.21 0.14 0.35 0.32 0.42 0.21 0.21 0.25 0.23 0.24 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 2 1 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 1 – 2 0 1 1 0 0 0 1 1 0 3 6 4 3 5 11
Bud Neck 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 1 – 6 2 5 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
Cytoplasm 1 8 7 3 10 9 18 – 12 17 7 5 20 47 45 51 46 33 1 4 2 5 16 15
Endoplasmic Reticulum 0 0 0 0 0 1 0 – 0 1 0 0 0 0 1 18 9 15 0 1 0 1 0 4
Endosome 3 2 3 0 3 3 9 – 0 0 0 2 3 9 9 6 2 1 8 5 4 3 8 5
Golgi 1 1 1 0 0 0 0 – 0 0 0 0 2 0 0 21 39 22 0 1 1 0 0 1
Mitochondria 17 4 0 6 7 33 45 – 95 67 93 91 1 0 0 2 6 4 3 3 2 9 9 10
Nucleus 133 106 91 113 84 101 147 – 151 177 106 149 120 133 130 179 124 120 149 135 82 120 126 121
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 2 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 2 0 0 4 2 5 1 – 2 6 6 7 0 0 1 0 2 1 9 6 1 5 6 8
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 0 0 1 2 5 – 2 5 0 6 0 3 1 0 0 0 3 3 1 7 9 14
Vac/Vac Membrane 42 71 22 9 21 23 27 – 15 11 5 9 38 46 28 124 91 109 47 46 35 47 61 76
Unique Cell Count 177 178 117 129 122 152 213 224 233 159 208 173 221 206 354 286 261 230 220 141 209 253 287
Labelled Cell Count 201 193 124 136 128 177 254 286 287 223 277 185 239 217 404 321 305 230 220 141 209 253 287


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.7 8.3 7.2 6.8 5.6 5.4 – 4.6 4.8 4.4 4.5 8.0 7.7 11.1 16.3 16.0 15.9 6.9 7.2 7.6
Std Deviation (1e-4) 2.0 2.1 2.3 1.8 1.9 1.4 1.3 – 0.9 0.9 1.6 1.0 1.5 1.6 30.1 4.1 3.8 3.7 1.5 1.6 1.7
Intensity Change (Log2) – – – -0.22 -0.3 -0.58 -0.62 – -0.87 -0.8 -0.92 -0.9 -0.06 -0.12 0.41 0.97 0.94 0.93 -0.27 -0.22 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.7 0 0.8 – -0.2 0.5 -0.6 0 1.6 3.6 3.7 2.4 2.7 1.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 2.3 3.9 2.8
Mitochondria 0 0 5.4 5.3 – 0 0 0 0 0 0 0 0 0 0
Nucleus 2.0 -1.6 -2.0 -1.7 – -2.0 -0.4 -2.0 -1.2 -1.6 -3.3 -2.7 -5.2 -6.3 -5.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.8 -0.3 -0.8 -1.5 – -3.4 -4.3 -4.3 -4.3 0.7 0.4 -1.2 3.3 2.6 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9166 2.8417 2.3737 2.4902 1.39 2.3741 5.4868 5.0992 5.9184 6.3201 4.7728 5.1581 3.024 3.6134 2.6102 2.3536 3.6418 3.1797
Actin 0.0373 0.0001 0.0165 0.0055 0.0067 0.0058 0 0.0001 0 0 0 0 0.0543 0.0001 0.0522 0.071 0.0094 0.0222
Bud 0.0006 0.0001 0.0005 0.0001 0.001 0.0004 0 0 0 0 0 0 0.0008 0.0001 0.0003 0.0005 0.0007 0.0005
Bud Neck 0.0013 0.0002 0.0013 0.0053 0.0095 0.0077 0 0.0002 0.0001 0 0.0002 0.0004 0.0035 0.0003 0.002 0.0155 0.0014 0.0014
Bud Periphery 0.0012 0.0001 0.0007 0.0002 0.003 0.001 0 0 0 0 0 0 0.0017 0.0001 0.0004 0.0007 0.0015 0.0006
Bud Site 0.0036 0.0004 0.0021 0.0001 0.0023 0.0011 0 0.0001 0 0 0 0 0.002 0.0003 0.0012 0.0023 0.0016 0.0002
Cell Periphery 0.0004 0 0.0002 0.0006 0.0008 0.0002 0 0 0 0 0 0 0.0003 0 0.0001 0.0002 0.0003 0.0001
Cytoplasm 0.0121 0.0006 0.0051 0.0015 0.0045 0.0011 0 0.0012 0.0001 0 0 0 0.0047 0.0006 0.0055 0.0021 0.0011 0.0023
Cytoplasmic Foci 0.0191 0 0.0126 0.0008 0.0241 0.0011 0 0.0001 0 0 0 0 0.0256 0.0005 0.0081 0.0043 0.0007 0.0006
Eisosomes 0.0004 0 0.0002 0.006 0.0002 0.0001 0 0 0 0 0 0 0.0004 0 0.0003 0.0004 0.0002 0.0001
Endoplasmic Reticulum 0.0059 0.0001 0.002 0.0011 0.0102 0.0014 0 0.0004 0 0 0 0 0.0049 0 0.0058 0.0021 0.0031 0.0004
Endosome 0.0199 0.0001 0.0166 0.0009 0.1198 0.0082 0 0.0007 0 0 0 0 0.0281 0.0001 0.0217 0.0141 0.0189 0.0024
Golgi 0.0054 0 0.0082 0.0012 0.023 0.003 0 0 0 0 0 0 0.0068 0 0.0115 0.0101 0.0053 0.0036
Lipid Particles 0.0197 0 0.0103 0.003 0.024 0.0014 0 0.0001 0 0 0 0 0.0206 0 0.0108 0.0042 0.0027 0.0019
Mitochondria 0.0144 0.0009 0.0068 0.001 0.0809 0.0143 0 0.0001 0 0 0 0.0001 0.0061 0.0008 0.0069 0.0041 0.0087 0.0041
None 0.0074 0 0.0009 0.0185 0.0015 0.0005 0 0.0001 0 0 0 0 0.0114 0.0003 0.0034 0.0019 0.0017 0.0012
Nuclear Periphery 0.0278 0.0063 0.0205 0.0094 0.034 0.0145 0.0005 0.0237 0.0011 0.0001 0.0009 0.0007 0.0231 0.0022 0.0073 0.0357 0.0193 0.0031
Nucleolus 0.016 0.0128 0.0137 0.0072 0.0645 0.0321 0.0107 0.0277 0.0115 0.0064 0.0446 0.0304 0.0221 0.0146 0.0121 0.0178 0.0749 0.0352
Nucleus 0.7771 0.9764 0.8604 0.9316 0.5402 0.8879 0.9862 0.9286 0.9844 0.9932 0.9497 0.966 0.7392 0.9719 0.8373 0.7988 0.841 0.9066
Peroxisomes 0.0058 0 0.0136 0.0007 0.0045 0.0002 0 0 0 0 0 0 0.0265 0 0.0043 0.002 0.0004 0.0007
Punctate Nuclear 0.0148 0.0014 0.0037 0.0046 0.0224 0.0132 0.0026 0.0163 0.0027 0.0002 0.0044 0.0022 0.0135 0.0076 0.0061 0.0091 0.0019 0.0118
Vacuole 0.0067 0.0003 0.0026 0.0004 0.0126 0.0029 0 0.0003 0 0 0 0.0001 0.0026 0.0003 0.0015 0.0013 0.0023 0.0004
Vacuole Periphery 0.0031 0.0002 0.0016 0.0003 0.01 0.0019 0 0.0003 0 0 0 0 0.0018 0.0002 0.0009 0.0017 0.0029 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.189 10.7717 9.1349 16.9343 10.4153 14.9104 19.0596 23.6277 19.5508 18.8525
Translational Efficiency 0.6471 0.6717 0.8354 0.4388 0.6025 0.6931 0.5623 0.5973 0.7012 0.6061

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Vhr1

Vhr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vhr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available