Standard name | |
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Human Ortholog | |
Description | Minor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 5 | 0 | – | 0 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 2 | 3 | 9 |
Bud | 0 | 2 | 2 | 0 | 1 | 4 | 6 | 3 | 7 | – | 6 | 8 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 5 | 8 | 20 |
Bud Neck | 0 | 0 | 6 | 1 | 5 | 1 | 1 | 3 | 3 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 2 | 2 | 6 | 7 | 8 | – | 5 | 8 | 1 | 3 | 6 | 15 | 5 | 11 | 0 | 0 | 2 | 2 | 5 | 5 |
Cytoplasm | 90 | 198 | 316 | 314 | 264 | 395 | 508 | 426 | 283 | – | 244 | 297 | 459 | 411 | 475 | 216 | 153 | 155 | 91 | 184 | 253 | 173 | 259 | 339 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 0 | 3 | 6 | 8 | 2 | – | 0 | 2 | 0 | 4 | 5 | 11 | 7 | 9 | 0 | 0 | 1 | 6 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 0 | 1 | 2 | 2 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 0 | 0 | 0 | 1 | 1 | 1 |
Mitochondria | 6 | 5 | 2 | 3 | 0 | 25 | 59 | 28 | 103 | – | 125 | 65 | 0 | 0 | 3 | 6 | 3 | 1 | 7 | 13 | 20 | 25 | 41 | 30 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 4 | – | 2 | 3 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | – | 1 | 3 | 2 | 4 | 8 | 0 | 0 | 0 | 1 | 1 | 4 | 2 | 1 | 6 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 2 | 11 | 20 | 13 | – | 7 | 13 | 3 | 8 | 1 | 4 | 5 | 9 | 1 | 4 | 3 | 6 | 16 | 15 |
Unique Cell Count | 95 | 204 | 320 | 318 | 265 | 408 | 548 | 471 | 346 | 319 | 358 | 465 | 429 | 489 | 222 | 164 | 169 | 110 | 215 | 305 | 236 | 355 | 459 | |
Labelled Cell Count | 99 | 208 | 331 | 319 | 273 | 438 | 602 | 505 | 425 | 390 | 408 | 469 | 434 | 505 | 255 | 180 | 194 | 110 | 215 | 305 | 236 | 355 | 459 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.0 | 4.7 | 4.4 | 5.1 | 4.7 | 5.0 | 5.6 | 4.6 | – | 4.4 | 5.0 | 4.8 | 6.7 | 8.9 | 6.9 | 7.3 | 7.1 | 4.0 | 3.9 | 4.4 |
Std Deviation (1e-4) | 1.3 | 0.9 | 1.0 | 1.8 | 1.7 | 1.9 | 1.6 | 1.8 | 1.6 | – | 1.4 | 1.3 | 2.1 | 4.3 | 6.2 | 2.4 | 2.4 | 2.3 | 1.8 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.14 | 0.01 | 0.1 | 0.27 | -0.01 | – | -0.08 | 0.12 | 0.03 | 0.52 | 0.94 | 0.57 | 0.65 | 0.61 | -0.23 | -0.24 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9479 | 1.9075 | 1.0848 | 0.7688 | 1.3495 | 1.3663 | -0.5616 | -0.1647 | -0.3142 | -0.3352 | -0.354 | -0.4584 | 0.1396 | 0.6355 | 0.6666 | 1.1552 | 0.5422 | 0.5129 |
Actin | 0.0159 | 0.0002 | 0.0049 | 0.0002 | 0.0247 | 0.0058 | 0.0473 | 0.0067 | 0.0077 | 0.0064 | 0.0019 | 0.0037 | 0.0093 | 0.0021 | 0.0055 | 0.0028 | 0.0031 | 0.0146 |
Bud | 0.0001 | 0.0001 | 0 | 0 | 0.0013 | 0 | 0.0037 | 0.0014 | 0.0026 | 0.0055 | 0.0123 | 0.0018 | 0.0205 | 0.0311 | 0.0304 | 0.0367 | 0.0315 | 0.0124 |
Bud Neck | 0.0023 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0005 | 0.0019 | 0.0012 | 0.001 | 0.0021 | 0.0008 | 0.0006 | 0.0018 | 0.0018 | 0.0046 | 0.001 | 0.0047 | 0.0053 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0026 | 0.0001 | 0.0068 | 0.0033 | 0.0038 | 0.0125 | 0.0259 | 0.0012 | 0.0221 | 0.0229 | 0.0225 | 0.034 | 0.0253 | 0.0176 |
Bud Site | 0.0014 | 0.0009 | 0.0007 | 0 | 0.0009 | 0.0003 | 0.0126 | 0.0304 | 0.0038 | 0.0051 | 0.0015 | 0.0003 | 0.0212 | 0.0949 | 0.0345 | 0.0042 | 0.0042 | 0.0012 |
Cell Periphery | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0006 | 0.0007 | 0.0007 | 0.0003 | 0.0001 | 0.0016 | 0.0017 | 0.0017 | 0.001 | 0.0006 | 0.0006 |
Cytoplasm | 0.0582 | 0.1398 | 0.1088 | 0.1522 | 0.2272 | 0.1851 | 0.1647 | 0.2352 | 0.2295 | 0.3352 | 0.2449 | 0.3201 | 0.0661 | 0.0814 | 0.0774 | 0.0769 | 0.0619 | 0.1468 |
Cytoplasmic Foci | 0.0152 | 0.0053 | 0.0045 | 0.0021 | 0.0234 | 0.0135 | 0.0445 | 0.0105 | 0.0257 | 0.0268 | 0.0116 | 0.0187 | 0.0315 | 0.0111 | 0.0088 | 0.0075 | 0.0159 | 0.021 |
Eisosomes | 0.001 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.001 | 0.0006 | 0.001 | 0.0004 | 0.0034 | 0.0006 | 0.006 | 0.0012 | 0.0033 | 0.0028 | 0.0017 | 0.0008 | 0.0037 | 0.0012 | 0.0021 | 0.0019 | 0.0014 | 0.002 |
Endosome | 0.0047 | 0.0019 | 0.0007 | 0.0002 | 0.0077 | 0.0016 | 0.017 | 0.0026 | 0.0222 | 0.0181 | 0.0566 | 0.0046 | 0.0117 | 0.0015 | 0.0011 | 0.0013 | 0.0079 | 0.0041 |
Golgi | 0.002 | 0.0003 | 0.0002 | 0 | 0.0065 | 0.0008 | 0.0102 | 0.0004 | 0.0057 | 0.004 | 0.0234 | 0.0036 | 0.0024 | 0.0003 | 0.0005 | 0.0002 | 0.002 | 0.0016 |
Lipid Particles | 0.0069 | 0.0002 | 0.0004 | 0.0001 | 0.017 | 0.0016 | 0.0201 | 0.0003 | 0.0052 | 0.0061 | 0.0013 | 0.0016 | 0.0088 | 0.0004 | 0.0016 | 0.0003 | 0.0323 | 0.0082 |
Mitochondria | 0.0011 | 0.0016 | 0.0003 | 0.0001 | 0.0043 | 0.0002 | 0.0151 | 0.0006 | 0.0025 | 0.0343 | 0.0093 | 0.0007 | 0.0098 | 0.0019 | 0.0167 | 0.0043 | 0.006 | 0.0024 |
None | 0.8539 | 0.846 | 0.8713 | 0.8436 | 0.5895 | 0.7783 | 0.6098 | 0.702 | 0.6709 | 0.5133 | 0.597 | 0.627 | 0.7194 | 0.6674 | 0.6493 | 0.669 | 0.7063 | 0.6692 |
Nuclear Periphery | 0.0018 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0015 | 0.0003 | 0.0012 | 0.0016 | 0.0011 | 0.0005 | 0.0073 | 0.0034 | 0.0069 | 0.0086 | 0.0077 | 0.0054 |
Nucleolus | 0.0033 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0017 | 0.0002 | 0.0006 | 0.0063 | 0.0001 | 0.0002 | 0.0072 | 0.0041 | 0.0102 | 0.0084 | 0.0056 | 0.0028 |
Nucleus | 0.0027 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0019 | 0.0008 | 0.0018 | 0.0046 | 0.002 | 0.0034 | 0.0202 | 0.0363 | 0.071 | 0.0684 | 0.0523 | 0.0389 |
Peroxisomes | 0.0065 | 0.0002 | 0.0049 | 0 | 0.0867 | 0.0094 | 0.0266 | 0.0005 | 0.0047 | 0.0041 | 0.0004 | 0.0052 | 0.0031 | 0.0014 | 0.0014 | 0.0005 | 0.0089 | 0.0027 |
Punctate Nuclear | 0.0204 | 0.0003 | 0.0003 | 0.0002 | 0.0015 | 0.001 | 0.0028 | 0.0006 | 0.0024 | 0.0024 | 0.0006 | 0.0047 | 0.025 | 0.0321 | 0.0492 | 0.0681 | 0.0177 | 0.041 |
Vacuole | 0.0007 | 0.0008 | 0.0004 | 0.0001 | 0.0008 | 0.0003 | 0.0038 | 0.001 | 0.0039 | 0.0062 | 0.0046 | 0.0009 | 0.0056 | 0.0026 | 0.0035 | 0.0034 | 0.0031 | 0.0016 |
Vacuole Periphery | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0006 | 0.0016 | 0.0025 | 0.0002 | 0.0012 | 0.0003 | 0.0007 | 0.0012 | 0.0011 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.0242 | 13.2273 | 7.0109 | 5.5832 | 14.2513 | 12.2533 | 12.5143 | 8.7687 | 5.3176 | 17.5987 |
Translational Efficiency | 0.9796 | 1.1156 | 0.5631 | 0.767 | 1.0125 | 1.315 | 0.7633 | 0.611 | 0.7495 | 0.8185 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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