Standard name
Human Ortholog
Description 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER33 has a paralog, SER3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.16 0.08 0.14 0.15 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.97 0.98 0.95 0.95 0.86 0.82 0.77 0.73 0.74 0.68 0.98 0.97 0.97 – – – 0.95 0.96 0.87 0.95 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.12 0.06 0.06 0 0.07 0 0 0 – – – 0 0 0.05 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.13 0.06 0.2 0.28 0.25 0.25 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 7 7 5 9 0 0 1 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 4 1 1 4 0 1 0 0 0 – – – 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 5 1 1 0 1 3 4 6 25 14 13 22 6 5 1 – – – 0 0 0 0 0 0
Cytoplasm 279 104 97 52 97 126 115 172 121 131 68 102 383 448 445 – – – 272 116 86 263 247 257
Endoplasmic Reticulum 3 2 1 1 3 1 6 25 9 10 4 11 14 12 12 – – – 3 1 4 8 9 19
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 2 0 2 1
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 2 0 0 1 1 7 17 12 31 50 23 37 0 0 0 – – – 1 0 1 0 2 1
Nucleus 0 1 0 1 5 0 1 3 0 1 0 1 0 1 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 – – – 0 0 0 0 1 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 1 0 1 0 2 2 0 0 1 0 0 0 – – – 3 1 0 1 2 4
Unique Cell Count 285 106 100 53 102 133 133 209 158 180 92 149 391 460 457 286 121 99 278 272 294
Labelled Cell Count 290 108 101 56 107 138 150 222 196 217 113 185 403 466 459 286 121 99 278 272 294


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.8 28.1 16.2 14.3 13.9 11.8 9.4 9.8 10.3 9.8 9.0 9.2 32.9 32.0 30.0 – – – 20.2 20.6 21.2
Std Deviation (1e-4) 5.4 7.1 4.4 5.1 4.0 3.4 2.9 2.8 2.4 2.6 2.3 2.2 8.2 7.9 7.7 – – – 4.4 6.0 6.0
Intensity Change (Log2) – – – -0.18 -0.22 -0.45 -0.79 -0.72 -0.66 -0.72 -0.85 -0.82 1.02 0.98 0.89 – – – 0.32 0.35 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 3.9 2.4 3.5 3.7 0 0 0 – – –
Cytoplasm 0.4 -0.7 -0.8 -2.8 -3.6 -4.4 -5.0 -4.6 -5.5 0.6 0.2 0.2 – – –
Endoplasmic Reticulum 0 0 0 0 3.2 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 3.7 0 4.7 5.8 5.3 5.4 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.8869 38.1251 35.5341 30.9748 31.259 36.1669 24.0235 35.9346 31.2833 27.0916 25.3475 30.3691 35.1417 42.2538 38.6805 46.7621 38.8324 40.4131
Actin 0.0321 0.0034 0.0005 0.0008 0.0031 0.0005 0.0399 0.0004 0.0142 0.0002 0.0019 0.0043 0.0025 0.003 0.0171 0.0002 0.0002 0.0013
Bud 0.0022 0.0003 0.0007 0.0008 0.0009 0.0004 0.0014 0.0003 0.0027 0.0007 0.0005 0.0038 0.0007 0.0011 0.0007 0.0017 0.0005 0.0007
Bud Neck 0.0016 0.0005 0.0005 0.0007 0.0006 0.0016 0.0009 0.0002 0.0006 0.0005 0.0006 0.0027 0.0004 0.0003 0.001 0.0004 0.0003 0.0014
Bud Periphery 0.0024 0.0001 0.0003 0.0005 0.0009 0.0002 0.002 0 0.0037 0.0002 0.0003 0.0065 0.0004 0.0007 0.0005 0.0004 0.0001 0.0002
Bud Site 0.0079 0.0006 0.0005 0.0003 0.0011 0.0001 0.005 0.0002 0.0051 0.0001 0.0003 0.0021 0.0008 0.005 0.0088 0.0002 0.0003 0.0001
Cell Periphery 0.0041 0.0011 0.0008 0.0002 0.0003 0.0002 0.0019 0.0001 0.0008 0.0002 0.0001 0.0011 0.0014 0.0015 0.0005 0.0002 0.0001 0.0002
Cytoplasm 0.5872 0.9646 0.9105 0.8758 0.8363 0.9156 0.7119 0.9768 0.8964 0.8395 0.7777 0.8561 0.7644 0.9506 0.8821 0.8852 0.8762 0.9229
Cytoplasmic Foci 0.0318 0.0023 0.0031 0.0243 0.0173 0.0045 0.0088 0.0022 0.0078 0.0054 0.0142 0.0121 0.014 0.0022 0.0183 0.0023 0.0041 0.0085
Eisosomes 0.0004 0 0 0 0.0001 0 0.0007 0 0 0 0 0 0.0001 0.0001 0.0006 0 0 0
Endoplasmic Reticulum 0.0936 0.0084 0.0198 0.0065 0.0197 0.0429 0.0712 0.0096 0.0098 0.0154 0.0701 0.026 0.0548 0.0032 0.0086 0.0019 0.0053 0.0362
Endosome 0.0356 0.0028 0.0092 0.0164 0.0253 0.0137 0.0277 0.004 0.0182 0.0215 0.0549 0.0285 0.0232 0.0054 0.0154 0.009 0.075 0.0128
Golgi 0.0108 0.0018 0.0007 0.002 0.0081 0.0017 0.0094 0.0005 0.0048 0.0005 0.0094 0.0088 0.0034 0.0007 0.005 0.0002 0.0024 0.0015
Lipid Particles 0.0092 0.0002 0.0002 0.0021 0.0115 0.0001 0.0022 0 0.0078 0.0001 0.0004 0.0032 0.0045 0.0005 0.0053 0 0.0008 0.0001
Mitochondria 0.0101 0.0003 0.0118 0.0101 0.0443 0.0034 0.0202 0.0002 0.0015 0.0003 0.0557 0.0117 0.0095 0.0019 0.0019 0.0002 0.0012 0.0007
None 0.0164 0.0002 0.0007 0.0059 0.0005 0.0003 0.0056 0.0002 0.0003 0.0002 0.0005 0.0017 0.048 0.0007 0.0009 0.0002 0.0004 0.0004
Nuclear Periphery 0.0403 0.0011 0.0029 0.0063 0.005 0.0021 0.0291 0.0006 0.0023 0.0044 0.0044 0.0044 0.0317 0.0022 0.0019 0.0022 0.0069 0.0015
Nucleolus 0.004 0 0.0003 0.0002 0.0004 0 0.001 0 0.0006 0 0 0.0003 0.0011 0.0025 0.0009 0 0.0001 0.0001
Nucleus 0.0188 0.001 0.0017 0.0031 0.0017 0.0012 0.0206 0.0005 0.0011 0.0021 0.0013 0.0018 0.0161 0.0042 0.0013 0.0016 0.0028 0.0014
Peroxisomes 0.0071 0.0001 0.0001 0.0017 0.0065 0 0.0006 0 0.0006 0 0.0002 0.0005 0.0005 0.0001 0.0031 0 0.0001 0.0001
Punctate Nuclear 0.0131 0 0.0001 0.0003 0.0002 0 0.0023 0 0.0013 0 0.0002 0.0004 0.0016 0.0002 0.0007 0 0.0003 0.0001
Vacuole 0.0568 0.0102 0.0298 0.0337 0.012 0.0087 0.0271 0.0035 0.0177 0.0935 0.0055 0.018 0.0134 0.0118 0.0224 0.0753 0.0169 0.0077
Vacuole Periphery 0.0143 0.0009 0.0058 0.0082 0.0041 0.0026 0.0103 0.0006 0.0028 0.0152 0.002 0.0059 0.0074 0.0021 0.0031 0.0189 0.0059 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.1696 41.2739 52.9484 81.0274 50.2236 51.6201 54.9943 74.4736 70.8963 68.6892
Translational Efficiency 1.445 1.5093 1.6252 1.0288 1.3102 1.999 1.4673 1.0411 1.4069 1.3607

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER33 has a paralog, SER3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ser33

Ser33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ser33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available