Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; relocalizes to the cytosol in response to hypoxia; cells defective in SDS3 display pleiotropic phenotypes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.14 0.14 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.32 0 0 0 0.09 0.16 0.09 0.24 0.18 0.26 0.16 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Nucleus 0.9 0.89 0.96 0.93 0.95 0.89 0.81 0.84 0.8 0.83 0.74 0.77 0.91 0.89 0.87 0.6 0.59 0.62 0.8 0.84 0.73 0.78 0.77 0.72
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.26 0.08 0.12 0.08 0.06 0.09 0.2 0.17 0.26 0.25 0.33 0.22 0.15 0.07 0 0 0 0 0.13 0.08 0.17 0.14 0.15 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.24 0.24 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 3 0 3 2 1 0 0 0 0 1 2 2 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 1 2 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4 11 3 15 9 6 7 1 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 2 1 9 17 10 16 19 9 5 11 8 8 52 62 46 48 35 0 0 0 0 1 1
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 1 1 2 15 16 10 0 0 0 0 2 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 2 1 2 1 26 15 21 1 0 0 2 5 3
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 1 1 0 1 0 2 1
Mitochondria 52 12 0 0 2 33 88 50 91 82 102 76 3 5 1 15 16 16 2 0 0 1 1 7
Nucleus 241 34 25 208 415 329 434 453 305 370 286 360 298 552 515 197 203 213 212 53 47 181 387 355
Nuclear Periphery 2 0 1 0 3 3 8 3 9 2 0 3 1 2 2 0 0 0 0 0 0 0 1 0
Nucleolus 70 3 3 17 25 32 105 92 101 112 126 103 49 42 22 4 8 8 35 4 11 33 73 78
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 4 5 2 5 8 1 7 2 0 14 19 11 10 8 5 1 0 1 1 6 5
Vac/Vac Membrane 0 0 0 1 0 0 3 3 1 2 1 4 1 2 3 66 82 82 6 2 2 6 12 26
Unique Cell Count 268 38 26 223 439 368 535 541 383 448 387 465 328 620 595 328 345 345 266 64 65 234 501 493
Labelled Cell Count 370 51 32 239 467 414 673 636 532 593 537 565 377 678 621 379 398 393 266 64 65 234 501 493


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.3 5.6 6.7 7.2 5.7 5.3 6.0 5.0 5.5 4.9 5.5 7.4 7.7 7.4 6.3 6.7 6.5 6.7 7.3 7.4
Std Deviation (1e-4) 0.9 1.6 1.0 1.4 1.8 1.4 1.2 1.9 0.9 1.2 1.1 1.1 1.2 1.7 1.6 1.3 1.5 1.3 1.2 1.4 1.7
Intensity Change (Log2) – – – 0.28 0.37 0.04 -0.08 0.11 -0.16 -0.02 -0.17 -0.02 0.41 0.48 0.42 0.19 0.26 0.23 0.26 0.39 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.8 0 3.0 0 0 0 0 0 0 0
Nucleus -0.6 -0.4 -1.1 -1.9 -1.7 -2.1 -1.8 -2.5 -2.3 -0.9 -1.2 -1.4 -3.7 -3.8 -3.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1.0 0 1.7 1.6 2.2 1.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8427 2.1245 2.3616 1.5766 1.9959 2.1545 2.9178 2.9696 2.8201 2.5511 2.5975 2.5289 2.4867 1.5189 1.5498 1.6853 1.7347 1.5266
Actin 0 0 0 0 0 0 0.0426 0 0.0213 0.0514 0.0073 0.0037 0.0182 0 0 0.0219 0.0374 0.0023
Bud 0.0001 0 0 0 0 0 0.0007 0 0.0004 0.0003 0.0002 0.0001 0.0003 0 0.0001 0.0017 0.0002 0
Bud Neck 0.0003 0.0001 0.0001 0 0.0002 0.0005 0.0064 0 0.0014 0.0033 0.0004 0.0005 0.0004 0 0.0001 0.0021 0.0009 0.0006
Bud Periphery 0.0002 0 0 0 0 0 0.0014 0.0001 0.0006 0.0005 0.0003 0.0001 0.0005 0 0.0002 0.0048 0.0004 0.0001
Bud Site 0.0002 0 0 0 0 0 0.0118 0.0001 0.0154 0.0015 0.0003 0.0001 0.001 0 0.0001 0.0106 0.0004 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0006 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.001 0.0001 0
Cytoplasm 0.0001 0 0 0 0 0.0002 0.0058 0 0.0003 0.0004 0.0073 0 0.0003 0 0.0001 0.0004 0.0002 0.0004
Cytoplasmic Foci 0.0001 0 0 0 0 0.0002 0.0337 0 0.0014 0.0182 0.0028 0.001 0.0095 0 0.0003 0.0103 0.0006 0.0003
Eisosomes 0 0 0 0 0 0 0.0005 0 0.0002 0.0004 0.0002 0 0.0001 0 0 0.0002 0.0005 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0055 0 0.0002 0.0003 0.0007 0.0001 0.0004 0 0.0001 0.0005 0.0013 0.0004
Endosome 0 0 0 0 0 0 0.0261 0.0001 0.002 0.0051 0.0125 0.0019 0.0059 0 0.0005 0.0071 0.004 0.0023
Golgi 0 0 0 0 0 0 0.009 0 0.0016 0.0084 0.0063 0.0021 0.0038 0 0.0001 0.0061 0.0047 0.0002
Lipid Particles 0 0 0 0 0 0 0.025 0.0003 0.002 0.0248 0.0157 0.0074 0.0061 0 0.0008 0.0147 0.0028 0.0003
Mitochondria 0.0038 0 0.0001 0 0.0001 0.0001 0.0071 0.0002 0.0069 0.0041 0.0144 0.0038 0.0016 0.0001 0.0009 0.0129 0.002 0.0002
None 0.0001 0 0 0 0 0.0001 0.0127 0 0.0004 0.0005 0.0426 0.0001 0.0008 0 0.0001 0.0005 0.0007 0.0006
Nuclear Periphery 0.0002 0.0007 0.0007 0.0002 0.002 0.0001 0.0132 0.0043 0.0014 0.0012 0.005 0.0005 0.0035 0.0001 0.0007 0.0016 0.0057 0.0059
Nucleolus 0.0472 0.04 0.0304 0.0255 0.2263 0.1342 0.0404 0.031 0.0203 0.0124 0.1326 0.0816 0.0507 0.0386 0.0492 0.0269 0.1622 0.0898
Nucleus 0.9379 0.9525 0.9629 0.9711 0.764 0.8634 0.7227 0.9597 0.9202 0.8293 0.734 0.8903 0.8876 0.9609 0.9443 0.8468 0.7725 0.8941
Peroxisomes 0 0 0 0 0 0 0.0187 0 0.0027 0.0333 0.0038 0.0057 0.007 0 0 0.0154 0.0005 0.0001
Punctate Nuclear 0.0092 0.0066 0.0057 0.0031 0.007 0.0011 0.0108 0.0036 0.0007 0.0042 0.0115 0.0007 0.0011 0.0003 0.0004 0.0014 0.0022 0.0022
Vacuole 0.0003 0 0 0 0 0 0.0037 0.0001 0.0003 0.0001 0.0006 0.0001 0.0006 0 0.0012 0.0104 0.0004 0.0001
Vacuole Periphery 0.0001 0 0 0 0 0 0.0017 0.0002 0.0002 0.0002 0.0013 0.0001 0.0004 0 0.0009 0.0027 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.4101 11.0266 7.4384 9.5202 7.2694 3.3622 11.9091 12.6553 10.5339 11.2675
Translational Efficiency 1.4702 1.5394 2.1071 1.8342 2.2604 4.6037 1.336 1.5633 1.5957 1.8089

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; relocalizes to the cytosol in response to hypoxia; cells defective in SDS3 display pleiotropic phenotypes
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sds3

Sds3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sds3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available