Standard name
Human Ortholog
Description Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.05 0 0 0.14 0 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.15 0.2 0.06 0.24 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.08 0.09 0.06 0.06 0.1 0.07 0.09 0.08 0.06 0.11 0.1 0.14 0.2 0.22 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.49 0.72 0.37 0.38 0.41 0.31 0.37 0.34 0.31 0.22 0.3 0.22 0.72 0.75 0.76 0 0 0 0.79 0.81 0.84 0.82 0.79 0.88
Endosome 0 0 0 0.05 0 0.07 0.06 0.11 0.07 0.11 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.44 0.4 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.1 0.06 0.24 0.2 0.23 0.37 0.31 0.39 0.33 0 0 0 0 0.11 0.24 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.23 0.3 0.4 0.26 0.13 0.17 0.16 0.07 0.09 0.06 0.07 0.08 0.09 0.1 0 0 0 0.08 0.14 0.12 0.09 0.08 0.07 0
Nucleolus 0 0 0 0 0 0 0 0.05 0.07 0.08 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0.43 0.17 0.36 0.3 0.41 0.31 0.28 0.21 0.13 0.21 0.14 0.18 0.14 0.13 0.05 0.32 0.17 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 2 0 3 0 15 17 14 14 18 23 3 3 4 10 0 2 1 1 11 2 5 1
Bud 0 0 0 2 0 5 6 8 17 13 19 25 0 0 0 2 0 0 1 1 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 3 7 3 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 10 3 5 8 6 6 6 8 9 16 5 5 24 15 1 6 0 0 0 0 0 0
Cytoplasm 8 5 9 18 24 17 24 40 22 34 35 26 7 8 22 15 4 1 0 0 0 0 1 0
Endoplasmic Reticulum 91 58 107 82 107 85 151 139 95 84 124 99 46 59 118 3 0 0 173 88 317 89 136 148
Endosome 0 1 4 10 10 18 26 43 22 44 28 48 1 1 3 0 0 0 3 0 2 1 2 1
Golgi 8 0 21 7 9 1 6 5 1 0 3 1 2 1 1 17 8 10 0 0 4 2 1 0
Mitochondria 4 1 16 21 15 67 81 92 115 121 164 153 0 0 0 3 2 6 1 0 5 2 4 1
Nucleus 1 0 1 0 2 1 2 3 3 6 5 7 0 0 1 2 0 0 0 0 0 0 0 0
Nuclear Periphery 43 24 114 56 35 46 65 28 28 25 28 37 6 8 2 0 0 2 31 12 33 8 12 6
Nucleolus 0 0 2 2 3 9 18 20 22 32 47 55 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 1 1 2 4 2 4 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 1 7 5 5 9 10 19 0 0 0 6 1 0 1 0 0 0 1 0
Vac/Vac Membrane 80 14 102 63 107 86 112 85 39 81 58 81 9 10 8 24 3 6 4 1 1 0 3 3
Unique Cell Count 186 81 287 213 262 275 406 403 308 388 419 459 64 79 156 74 18 25 221 109 378 110 173 170
Labelled Cell Count 237 105 389 264 321 345 520 493 394 482 553 602 79 95 183 97 19 34 221 109 378 110 173 170


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 13.5 12.0 11.6 10.9 9.3 9.7 9.7 9.1 8.8 8.9 9.1 11.9 11.5 12.0 11.7 15.1 16.4 12.2 13.1 14.1
Std Deviation (1e-4) 2.1 2.0 1.9 2.1 1.8 1.6 1.6 1.7 1.7 1.7 1.8 1.7 1.8 1.9 1.4 2.4 2.9 2.6 2.2 2.3 1.8
Intensity Change (Log2) – – – -0.04 -0.14 -0.37 -0.31 -0.3 -0.39 -0.44 -0.44 -0.39 -0.01 -0.06 0.01 -0.04 0.34 0.46 0.02 0.12 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.5 2.8 2.9 2.5 2.8 3.2 0 0 0 0 0 0
Bud 0 0 0 0 0 4.0 3.1 3.7 4.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -1.1 -0.4 -1.7 -1.7 -1.2 -1.1 -1.1 0 0 0 4.5 5.1 0 0
Cytoplasm 2.6 3.0 1.7 1.7 3.4 2.2 3.0 2.8 1.6 0 0 4.3 0 0 0
Endoplasmic Reticulum 0.3 0.9 -1.6 0 -0.8 -1.7 -4.5 -2.1 -4.7 5.0 5.9 7.7 -5.5 -3.2 -3.8
Endosome 2.2 1.8 3.1 3.2 4.8 3.4 5.0 3.3 4.7 0 0 0 0 0 0
Golgi -1.9 -2.0 -4.2 -3.9 -4.1 -4.5 -5.4 -4.8 -5.6 0 0 -3.1 3.9 0 0
Mitochondria 1.8 0.1 6.3 5.4 6.1 9.3 8.2 10.1 8.8 0 0 -3.0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -3.1 -6.9 -6.0 -7.0 -10.5 -8.8 -10.6 -10.8 -10.5 -4.6 -4.9 -8.8 -6.6 -3.4 -3.1
Nucleolus 0 0 2.2 2.9 3.1 4.0 4.4 5.4 5.6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Vacuole -1.4 1.3 -1.1 -2.2 -4.2 -6.6 -4.2 -6.8 -5.5 -3.3 -3.9 -7.1 -0.5 -1.6 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.8496 9.172 8.7303 8.6831 7.9633 9.018 11.8533 11.9969 11.3402 10.7714 11.6652 11.5715 11.1955 11.4196 11.4859 11.0696 11.312 11.1669
Actin 0.0077 0.0022 0.0081 0.0054 0.0013 0.0038 0.0006 0.0008 0.0002 0.0001 0.0004 0.0002 0.0051 0.0007 0.0005 0.0484 0.0031 0.0013
Bud 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0 0 0.0001 0 0 0.0003 0.0001 0
Bud Neck 0.0006 0.0006 0.0004 0.0027 0.0021 0.0015 0.0001 0.0002 0 0.0003 0 0.0001 0.0002 0.0001 0.0002 0.0012 0.0004 0.0003
Bud Periphery 0.0002 0.0002 0.0001 0.0004 0.0002 0.0002 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0001 0 0.0008 0.0003 0.0001
Bud Site 0.0003 0.0038 0.0006 0.0007 0.0003 0.0001 0.0001 0.0001 0 0 0 0 0.0002 0 0 0.0011 0.0001 0
Cell Periphery 0.0002 0.0006 0.0003 0.0005 0.0002 0.0002 0.0002 0.0028 0.0002 0.0006 0.0001 0.0002 0.0004 0.0008 0.0004 0.0011 0.0002 0.0002
Cytoplasm 0.0124 0.0512 0.0271 0.0289 0.0103 0.0152 0.0016 0.0052 0.0013 0.0015 0.0009 0.0005 0.007 0.0033 0.0064 0.0034 0.0024 0.0008
Cytoplasmic Foci 0.005 0.0068 0.0054 0.01 0.0044 0.0008 0.0012 0.0012 0.0003 0.0001 0.0005 0.0002 0.0042 0.0008 0.0007 0.0069 0.0014 0.0003
Eisosomes 0.0001 0 0.0005 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0.0004 0.0001 0
Endoplasmic Reticulum 0.2762 0.5547 0.6361 0.6323 0.4806 0.4346 0.5776 0.7467 0.8426 0.948 0.7507 0.5778 0.4487 0.6868 0.8078 0.795 0.5816 0.5463
Endosome 0.0248 0.0193 0.0177 0.0285 0.028 0.0061 0.008 0.0076 0.0037 0.0039 0.0044 0.002 0.0214 0.0075 0.0064 0.0123 0.0214 0.0062
Golgi 0.0045 0.0026 0.0024 0.0092 0.0032 0.0013 0.0015 0.0014 0.0006 0.0002 0.0012 0.0006 0.0062 0.0027 0.0012 0.0071 0.0065 0.002
Lipid Particles 0.0236 0.0191 0.0143 0.0096 0.0331 0.0089 0.0132 0.0268 0.0065 0.001 0.0057 0.0104 0.0128 0.046 0.0143 0.0085 0.0126 0.006
Mitochondria 0.007 0.0006 0.0016 0.0028 0.011 0.0009 0.0016 0.0002 0.0007 0.0002 0.0005 0.0002 0.0011 0.0005 0.0002 0.0022 0.0021 0.0004
None 0.0007 0.0005 0.0082 0.0002 0.0013 0.0003 0.004 0.0001 0.0003 0 0.0001 0.0001 0.003 0.0002 0.0001 0.002 0.0004 0.0003
Nuclear Periphery 0.5642 0.2725 0.2257 0.2401 0.3475 0.4566 0.3679 0.1944 0.1361 0.0326 0.2245 0.3966 0.4383 0.2356 0.1507 0.0942 0.323 0.4181
Nucleolus 0.0043 0.0011 0.004 0.0001 0.0005 0.0023 0.0003 0.0001 0 0 0 0 0.0007 0 0 0.0001 0.0003 0
Nucleus 0.0388 0.0309 0.0234 0.0149 0.0586 0.0472 0.0042 0.0018 0.0009 0.0008 0.0043 0.003 0.0162 0.002 0.0025 0.0016 0.0244 0.0062
Peroxisomes 0.0024 0.0002 0.0082 0.0003 0.0004 0.0007 0.0002 0 0 0 0 0 0.0013 0 0 0.0021 0.0001 0
Punctate Nuclear 0.0062 0.0135 0.0043 0.001 0.0018 0.0055 0.002 0.0026 0.0001 0 0.0008 0.0003 0.017 0.0004 0.0005 0.0031 0.0052 0.0005
Vacuole 0.0067 0.0139 0.006 0.0067 0.0077 0.0063 0.006 0.0038 0.0025 0.0094 0.0016 0.0015 0.005 0.0048 0.0034 0.0031 0.0047 0.0023
Vacuole Periphery 0.0139 0.0055 0.0055 0.0052 0.0075 0.0074 0.0096 0.0043 0.0038 0.0014 0.0042 0.0063 0.0109 0.0079 0.0046 0.0051 0.0097 0.0086

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.9123 28.0676 29.2575 30.3572 30.0467 17.1845 28.0413 31.4673 27.7332 34.1005
Translational Efficiency 0.5716 0.486 0.5156 0.4347 0.4538 0.9387 0.5078 0.4557 0.5464 0.4503

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
Localization
Cell Percentages ER (88%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Ice2

Ice2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ice2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available