Standard name
Human Ortholog
Description Homo-isocitrate dehydrogenase; an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 – – – 0.07 0.08 0.06 0.14 0.09 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0.05 0.06 0 0 0.06 0.06 0.12 0.06 0.06 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0.07 0.11 0.06 0.12 0 0 0 0 0 0 0 0.06 0.17 0.5 – – – 0.13 0.13 0.07 0.14 0.15 0.13
Mitochondria 0.93 0.86 0.71 0.86 0.83 0.86 0.86 0.83 0.91 0.88 0.89 0.83 0.8 0.62 0.61 – – – 0.68 0.63 0.64 0.52 0.59 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.07 0 0.05 0.05 0 0.1 0.1 0.08 0.11 0.1 0.12 0.06 0.1 0.14 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0.07 0.25 0.11 0 0 0 0.07 0 0 0 0 0.06 0 0.06 – – – 0.07 0 0.12 0.09 0.09 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0.07 0.05 0 0 0 0 0 0.06 0.14 0.17 0.06 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 7 1 7 2 3 2 3 0 0 1 4 1 1 – – – 6 14 15 9 10 12
Bud 2 1 6 1 8 6 10 10 4 9 8 6 0 0 0 – – – 0 4 2 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 1 0 0 0
Bud Site 0 1 0 0 1 0 2 2 2 1 0 0 0 0 0 – – – – – – – – –
Cell Periphery 1 4 6 9 7 1 2 1 2 3 0 4 0 1 0 – – – 0 0 0 0 0 0
Cytoplasm 3 1 6 5 20 8 15 17 10 11 12 15 6 5 1 – – – 0 3 0 1 0 3
Endoplasmic Reticulum 1 0 0 0 2 0 2 1 2 2 1 0 0 0 0 – – – 0 1 1 0 0 0
Endosome 0 1 4 1 2 3 2 1 1 0 1 3 1 0 0 – – – 1 4 6 1 1 2
Golgi 0 10 22 11 35 3 5 1 0 2 0 2 3 13 9 – – – 12 24 16 9 17 13
Mitochondria 91 127 149 158 232 203 278 251 241 269 191 210 39 48 11 – – – 67 122 162 35 71 58
Nucleus 0 1 1 0 1 2 2 1 2 2 3 0 0 1 0 – – – 0 0 0 0 0 0
Nuclear Periphery 2 1 3 0 0 3 3 8 8 5 10 13 0 0 0 – – – 0 0 1 0 0 0
Nucleolus 7 3 10 9 9 24 33 25 30 30 25 16 5 11 0 – – – 0 2 4 0 3 0
Peroxisomes 3 11 52 20 0 9 11 21 7 7 2 7 3 3 1 – – – 6 6 29 6 11 8
SpindlePole 0 0 6 2 1 4 4 6 1 1 0 1 0 1 0 – – – 0 1 5 0 0 1
Vac/Vac Membrane 3 9 3 5 21 12 8 9 4 9 0 15 7 13 1 – – – 0 3 0 0 2 0
Unique Cell Count 98 147 209 184 281 236 323 301 266 305 214 253 49 78 18 100 194 253 69 121 105
Labelled Cell Count 113 174 275 222 346 280 380 356 317 351 253 293 68 97 24 100 194 253 69 121 105


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.8 25.6 19.5 23.4 23.9 21.6 18.7 18.2 17.8 17.4 15.6 17.2 22.3 24.4 26.2 – – – – 25.6 23.4
Std Deviation (1e-4) 8.5 8.8 6.5 6.8 7.2 8.6 6.7 6.3 6.4 5.4 5.1 6.3 9.9 7.7 6.4 – – – – 9.5 8.2
Intensity Change (Log2) – – – 0.26 0.29 0.15 -0.06 -0.1 -0.14 -0.16 -0.33 -0.18 0.19 0.32 0.43 – – – – 0.39 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -0.6 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 -0.2 0.1 0.3 0 0.1 0.5 -0.3 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 1.0 -0.3 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm -0.1 2.1 0.3 1.0 1.5 0.5 0.5 1.4 1.6 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi -1.6 0.7 -4.2 -4.6 -5.5 -5.4 -5.2 -4.9 -4.7 0 1.4 0 – – –
Mitochondria 3.5 3.0 3.8 4.2 3.3 5.4 4.8 4.7 3.0 1.2 -1.6 -0.9 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 1.9 2.2 0 0 0 – – –
Nucleolus 0 -0.9 2.1 2.2 1.5 2.5 2.1 2.6 0.7 0 2.7 0 – – –
Peroxisomes -3.6 -8.8 -6.4 -7.5 -5.7 -7.3 -7.9 -7.4 -7.1 -2.9 -4.0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 3.1 2.1 0 0 0 0 0 2.5 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 51.0215 60.9299 52.881 48.3265 46.8466 54.9878 50.1333 52.2445 49.1541 41.0061 40.9784 50.0094 38.4665 52.0755 39.3526 47.8556 48.5255 43.1637
Actin 0.0293 0.0044 0.0037 0.0067 0.0054 0.0012 0.0037 0.0018 0.0012 0.0003 0.0105 0.0002 0.0095 0.0066 0.0015 0.0002 0.0001 0.0011
Bud 0.0003 0.0002 0.0004 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0 0 0 0 0
Bud Neck 0.0003 0.0001 0.0001 0 0.0001 0.0055 0.0002 0.0002 0.0002 0 0.0002 0 0.0002 0 0 0 0 0.0001
Bud Periphery 0.0004 0.0003 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0004 0.0002 0 0.0005 0 0 0 0 0
Bud Site 0.0023 0.0007 0.0013 0 0.0002 0.0001 0.0014 0.0018 0.0006 0.0001 0.0004 0 0.0011 0.0001 0.0001 0 0 0
Cell Periphery 0.0002 0.0008 0.0003 0.0001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002 0 0.0007 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.0006 0 0.0002 0 0 0.0003 0.0001 0 0 0.0001 0.002 0.0062 0.0001 0 0 0.1246 0 0
Cytoplasmic Foci 0.0053 0.0033 0.0089 0.0001 0.0018 0.0007 0.0123 0.0038 0.0086 0.0001 0.0147 0.0004 0.0031 0.0009 0.001 0 0 0.0001
Eisosomes 0.0019 0.0014 0.0006 0.0023 0.0006 0.0027 0.0024 0.0007 0.0017 0.0002 0.0005 0.0003 0.0005 0.0013 0.0005 0.001 0.0006 0.001
Endoplasmic Reticulum 0.0003 0 0.0001 0 0.0001 0.0003 0.0002 0 0 0.0006 0.0013 0.0002 0.0001 0 0 0 0 0
Endosome 0.0043 0.0003 0.002 0.0001 0.0031 0.0038 0.0023 0.0009 0.0012 0.0112 0.0325 0.0001 0.002 0.0002 0.0002 0 0 0
Golgi 0.0031 0.001 0.0033 0.0003 0.0063 0.0065 0.0024 0.0045 0.0028 0.0003 0.0279 0.0004 0.0025 0.001 0.0012 0 0.0002 0.0001
Lipid Particles 0.0225 0.0194 0.0124 0.0013 0.0095 0.0062 0.0212 0.0127 0.009 0.0009 0.0357 0.0056 0.0276 0.0221 0.0322 0.0002 0.0031 0.0021
Mitochondria 0.8937 0.93 0.945 0.9527 0.9585 0.9327 0.9269 0.9405 0.9542 0.9498 0.8346 0.9771 0.9168 0.9359 0.9365 0.87 0.9939 0.9924
None 0.0005 0 0.0001 0 0.0002 0.0001 0.0004 0 0.0001 0.0001 0.0033 0.0004 0.0002 0 0 0 0 0
Nuclear Periphery 0.0016 0.0001 0.0003 0 0.0003 0.0066 0.0051 0.0001 0 0.0073 0.0049 0.0006 0.0002 0 0 0.0001 0 0
Nucleolus 0.0079 0.0013 0.0015 0.0001 0.0054 0.0117 0.0056 0.001 0.0037 0.0002 0.0007 0.0001 0.0077 0.0033 0.0003 0 0 0.0002
Nucleus 0.001 0.0002 0.0004 0.0001 0.0006 0.0016 0.0012 0.0001 0.0002 0.0009 0.0006 0.0032 0.0007 0.0001 0.0001 0.0002 0 0
Peroxisomes 0.0076 0.0135 0.0068 0.0033 0.0037 0.0025 0.0076 0.0125 0.0062 0.0009 0.0124 0.0015 0.0108 0.0132 0.0211 0.0036 0.0004 0.0023
Punctate Nuclear 0.001 0 0.0002 0 0.0001 0.0007 0.0013 0 0.0001 0 0.0058 0.0002 0.0003 0 0 0 0 0
Vacuole 0.0021 0.0029 0.0017 0.0034 0.0004 0.0014 0.0006 0.0016 0.001 0.0143 0.0014 0.0001 0.009 0.0008 0.0005 0 0.0001 0
Vacuole Periphery 0.0139 0.02 0.01 0.0294 0.0031 0.0149 0.0048 0.0175 0.0088 0.0122 0.01 0.0033 0.0062 0.0143 0.0046 0.0001 0.0014 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 306.5012 357.0752 390.8893 414.29 325.6053 407.1245 646.1158 654.7301 587.6574 483.2821
Translational Efficiency 1.3595 1.4936 1.3657 1.297 1.3417 1.6399 1.3889 1.2487 1.2708 1.1783

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Homo-isocitrate dehydrogenase; an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Lys12

Lys12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lys12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available