Standard name
Human Ortholog
Description Methyltransferase required for m3U2634 methylation of the 25S rRNA; S-adenosylmethionine-dependent; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0.05 0.05 0.05 0.05 0 0.06 0 0.13 0.1 0.21 0.05 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0.1 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0 0.09 0.07 0.4 0.55 0.37 0.63 0.52 0.72 0.45 0.11 0 0 0 0 0.05 0 0 0 0 0.06 0.08
Nucleus 0.95 0.91 0.96 0.65 0.78 0.68 0.61 0.61 0.63 0.67 0.57 0.69 0.64 0.51 0.37 0.6 0.53 0.53 0.89 0.85 0.8 0.58 0.32 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleolus 0 0.06 0 0.29 0.09 0.07 0.14 0.13 0.11 0.07 0.08 0.07 0.3 0.46 0.39 0.35 0.35 0.41 0 0 0.07 0.16 0.19 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.1 0.07 0.06 0 0.07 0 0 0 0 0.09 0.13 0.18 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.1 0.19 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 1 2 5 4 6 5 8 3 0 0 0 0 0 0 2 0 1 0 1 2
Bud Neck 0 0 0 0 1 1 0 6 0 4 0 0 0 2 0 0 0 0 0 0 0 0 0 3
Bud Site 0 1 0 1 0 9 6 6 16 13 11 13 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 4 7 7 30 16 22 21 24 12 18 9 15 18 43 9 18 12 0 0 0 1 1 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1 0 1 2 1 3 2 2 3 0 0 0 0 1 0
Endosome 0 0 0 4 12 6 5 4 1 3 0 7 0 5 18 3 3 2 3 0 2 2 15 12
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 1 3 0 1 1 0 0 1 0
Mitochondria 67 2 6 21 27 129 249 156 274 212 224 181 13 3 3 3 8 8 1 0 2 1 8 15
Nucleus 279 117 188 157 300 219 276 256 274 271 178 277 74 94 74 112 112 81 263 107 153 42 48 58
Nuclear Periphery 2 0 1 1 1 0 4 8 16 2 3 6 0 2 0 3 2 0 1 0 0 0 3 9
Nucleolus 3 8 2 69 35 23 64 56 46 30 25 29 35 86 79 65 73 63 9 4 13 11 28 25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 1 0 1 23 26 19 13 31 11 13 3 9 10 24 36 5 5 2 0 0 0 3 6 9
Vac/Vac Membrane 3 6 0 2 11 4 5 7 9 12 5 14 1 1 1 7 9 5 6 10 12 7 29 33
Unique Cell Count 293 128 196 241 387 322 451 419 437 405 313 402 116 186 201 187 210 153 295 127 192 73 152 185
Labelled Cell Count 359 138 205 286 444 428 649 555 677 578 475 549 151 237 258 210 236 177 295 127 192 73 152 185


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.7 6.0 5.8 5.9 4.6 4.3 4.7 3.9 4.3 3.7 4.5 5.8 6.6 6.5 11.2 10.8 10.3 5.9 6.9 7.3
Std Deviation (1e-4) 0.8 1.5 1.1 1.1 1.8 1.2 1.2 1.1 0.8 0.8 0.8 1.0 1.2 1.7 2.1 2.4 2.5 2.5 1.4 2.0 1.8
Intensity Change (Log2) – – – -0.05 -0.03 -0.4 -0.49 -0.36 -0.64 -0.48 -0.69 -0.43 -0.07 0.13 0.12 0.89 0.84 0.77 -0.04 0.21 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 2.0 0.7 0.7 0.8 1.0 -0.4 1.1 -0.9 3.1 2.4 5.4 0.6 2.1 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 4.3 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 1.9 0 0 0 0 0 0 0 2.9 0 0 0 0.4 1.0
Nucleus -7.8 -5.7 -7.5 -9.0 -9.0 -8.7 -7.8 -9.5 -7.5 -7.5 -10.1 -12.4 -8.6 -9.8 -9.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 7.8 3.8 0 0 0 0 0 0 0 7.7 10.5 9.5 8.7 8.8 9.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.1 3.4 0 0 0 0 0 0 0 0 4.9 6.0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5699 1.6636 1.4191 1.1878 0.9356 1.4734 2.4888 2.0407 1.6432 1.2621 1.5633 1.8026 1.2027 1.6286 1.6625 1.6944 1.3534 1.4441
Actin 0.0057 0 0.0011 0 0.0002 0.0002 0.0464 0.0001 0.0071 0.0342 0 0.0017 0.0068 0.0001 0.0039 0.0359 0.0282 0.0001
Bud 0.0003 0.0001 0.0002 0 0 0 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002
Bud Neck 0.001 0.0005 0.0021 0.0003 0.0029 0.0009 0.0079 0.0005 0.0009 0.0042 0.0012 0.0014 0.0008 0.0005 0.0009 0.0006 0.0006 0.0016
Bud Periphery 0.0003 0.0001 0.0005 0.0001 0.0001 0.0001 0.0005 0.0003 0.0004 0.0002 0.0001 0.0002 0.0004 0.0002 0.0004 0.0003 0.0002 0.0003
Bud Site 0.0005 0.0003 0.0024 0.0002 0.0002 0.0001 0.0013 0.0007 0.0013 0.0007 0.0001 0.0002 0.0011 0.0004 0.0005 0.0006 0.0003 0.0002
Cell Periphery 0.0001 0.0001 0.0005 0 0 0 0.0002 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0002 0 0.0001
Cytoplasm 0.0015 0.0002 0.0011 0.0001 0.0009 0.0001 0.0094 0.0001 0.0021 0.0003 0.0002 0.0016 0.0022 0.0002 0.0011 0.0005 0.0008 0.0034
Cytoplasmic Foci 0.0024 0 0.0015 0.0001 0.0001 0.0002 0.0084 0.0001 0.0067 0.001 0 0.0057 0.0034 0 0.003 0.0018 0.0083 0.0003
Eisosomes 0.0001 0 0.0001 0 0 0 0.0003 0 0.0001 0.0003 0 0 0.0002 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0009 0 0.0005 0 0.0002 0.0001 0.0053 0 0.0019 0.0004 0 0.0008 0.0013 0 0.0007 0.0015 0.0004 0.0001
Endosome 0.0057 0.0003 0.0024 0.0001 0.0004 0.0009 0.0347 0.0003 0.008 0.0016 0.0001 0.0103 0.0086 0.0001 0.0035 0.0033 0.0053 0.0002
Golgi 0.0013 0 0.0009 0 0 0.0001 0.0084 0.0001 0.003 0.0019 0 0.0007 0.0024 0 0.0015 0.0033 0.0078 0
Lipid Particles 0.0077 0.0002 0.0081 0.0005 0.0001 0.0012 0.0105 0.0006 0.0064 0.0013 0 0.0019 0.0061 0.0002 0.0016 0.0087 0.0143 0.0002
Mitochondria 0.0017 0.0004 0.0016 0.0003 0.0002 0.0002 0.0031 0.001 0.0038 0.0005 0.0005 0.0004 0.0095 0.0006 0.0013 0.002 0.0016 0.0002
None 0.0014 0.0001 0.0004 0.0002 0.0003 0.0001 0.0064 0.0002 0.0029 0.0004 0.0003 0.0017 0.0036 0.0001 0.0006 0.0008 0.0005 0.0001
Nuclear Periphery 0.0189 0.0066 0.0137 0.0009 0.0042 0.0073 0.0409 0.0073 0.0092 0.0027 0.0044 0.0156 0.0058 0.0035 0.005 0.0278 0.0048 0.0011
Nucleolus 0.194 0.268 0.2859 0.2153 0.3031 0.2846 0.2306 0.3074 0.3086 0.2508 0.1074 0.3109 0.2268 0.3595 0.3869 0.2133 0.0704 0.3221
Nucleus 0.7146 0.7171 0.6201 0.7515 0.6784 0.6885 0.5584 0.6719 0.617 0.6947 0.8802 0.6267 0.6972 0.63 0.5593 0.6591 0.8104 0.6534
Peroxisomes 0.0019 0 0.0012 0.0001 0 0.0001 0.0027 0.0003 0.0083 0.0008 0 0.0005 0.0087 0 0.0037 0.0061 0.0168 0
Punctate Nuclear 0.0374 0.0048 0.0514 0.0301 0.0083 0.0147 0.0201 0.0072 0.0098 0.0035 0.0046 0.0179 0.0126 0.0031 0.0234 0.032 0.0283 0.0157
Vacuole 0.0015 0.0007 0.0028 0.0002 0.0003 0.0002 0.0021 0.0009 0.0016 0.0003 0.0004 0.0012 0.0014 0.0006 0.0015 0.0008 0.0005 0.0006
Vacuole Periphery 0.0012 0.0003 0.0014 0.0001 0.0001 0.0003 0.0018 0.0005 0.0007 0.0001 0.0004 0.0006 0.0009 0.0005 0.0007 0.0011 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.6452 17.5616 10.8596 14.1196 19.1031 11.9987 11.5911 13.3161 8.5148 18.2776
Translational Efficiency 1.6264 1.51 1.8782 1.1444 1.1501 1.8138 1.7445 0.814 1.4068 1.1037

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Methyltransferase required for m3U2634 methylation of the 25S rRNA; S-adenosylmethionine-dependent; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Bmt5

Bmt5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bmt5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available