Standard name
Human Ortholog
Description Transcriptional repressor; binds promoter sequences of cyclin genes, CYS3, and SMF2; not expressed during log phase of growth, but induced by stress or starvation during mitosis, and late in meiosis; represses 15% of all yeast genes as cells transition to quiescence; important for maintaining G1 arrest and for longevity of quiescent cells; member of Swi4p/Mbp1p family; phosphorylated by Cdc28p; relative distribution to nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.91 0.98 0.95 0.52 0.17 0.11 0.05 0 0 0 0 0.85 0.84 0.76 0.59 0.6 0.66 0.77 0.67 0.67 0.67 0.83 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.23 0.16 0.17 0.21 0.13 0.27 0.13 0 0 0 0.09 0 0.05 0.09 0.09 0.13 0.13 0 0
Nucleus 0 0 0 0 0.34 0.65 0.78 0.87 0.85 0.84 0.73 0.81 0.08 0.1 0.15 0.17 0.17 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.08 0.15 0.16 0.28 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0 0 0 0 0 0 0 0.06 0.07 0.08 0.16 0.24 0.19 0 0.1 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 3 3 1 1
Bud 0 0 0 0 2 0 2 4 1 1 7 2 0 0 0 0 1 0 1 3 1 1 3 2
Bud Neck 0 1 5 1 5 1 1 4 0 3 0 1 0 1 0 2 0 0 1 3 0 0 0 0
Bud Site 0 0 0 0 0 3 3 5 10 9 17 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 2 2 1 0 2 2 0 1
Cytoplasm 253 277 225 158 99 40 32 18 6 6 9 5 161 181 176 245 200 268 200 239 139 139 190 174
Endoplasmic Reticulum 3 3 1 2 0 0 2 0 0 0 0 0 0 0 1 22 22 30 2 2 3 3 0 1
Endosome 0 0 1 2 4 1 2 0 3 0 0 0 2 5 9 29 17 22 3 4 3 3 1 3
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 2 0 1 0 0 1 0
Mitochondria 7 8 0 3 7 54 47 63 67 45 120 47 1 1 0 36 7 21 22 33 27 27 7 8
Nucleus 0 7 2 2 66 152 221 332 276 293 325 288 16 21 35 72 56 50 0 9 4 4 3 0
Nuclear Periphery 0 0 0 0 0 0 3 0 6 2 3 2 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 3 5 31 49 57 122 92 0 1 0 2 2 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
SpindlePole 0 3 1 0 6 4 3 15 6 5 3 3 0 0 0 4 1 9 3 9 5 5 7 4
Vac/Vac Membrane 4 12 1 0 19 5 9 0 2 0 1 8 12 15 18 68 80 79 8 35 5 5 5 3
Unique Cell Count 263 306 229 166 192 233 285 381 326 349 443 357 189 216 231 418 334 408 259 360 207 207 229 211
Labelled Cell Count 268 311 236 172 210 263 331 472 426 421 607 452 192 225 240 485 390 485 259 360 207 207 229 211


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 4.9 4.7 5.5 4.8 5.2 5.8 5.5 6.2 5.8 6.2 5.3 5.5 5.3 5.8 5.9 5.4 4.9 5.0 5.2
Std Deviation (1e-4) 0.4 1.1 1.3 1.9 1.6 1.5 1.3 1.5 1.9 1.7 2.1 2.2 0.8 1.7 1.3 1.5 1.5 1.4 1.3 1.9 1.7
Intensity Change (Log2) – – – -0.05 0.16 -0.02 0.1 0.26 0.18 0.34 0.24 0.35 0.11 0.19 0.13 0.25 0.27 0.15 0.0 0.04 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.2 7.8 6.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8209 -0.9857 -0.9827 -0.7621 -1.3474 -1.0037 -0.3602 -0.281 -0.1486 -0.7928 0.0937 -0.2352 -0.9381 -0.4407 -0.7523 -0.7882 -0.9834 -1.0449
Actin 0.0496 0.0029 0.011 0.0002 0.0012 0.0009 0.0646 0.0003 0.0116 0.0232 0.0548 0.0039 0.0321 0.0015 0.0104 0.0371 0.0377 0.0012
Bud 0.0013 0.0002 0.0005 0.0001 0.002 0 0.001 0.001 0.0006 0.0061 0.0009 0.0008 0.0004 0.0014 0.0023 0.0003 0.0005 0.0007
Bud Neck 0.0173 0.0005 0.0005 0.0002 0.0001 0.0003 0.0022 0.0012 0.0007 0.0004 0.0014 0.0031 0.0014 0.0002 0.0011 0.0037 0.0004 0.002
Bud Periphery 0.0035 0.0003 0.001 0.0001 0.0082 0 0.0019 0.0016 0.0009 0.0242 0.0011 0.0013 0.0006 0.001 0.0009 0.0007 0.001 0.0005
Bud Site 0.0102 0.0063 0.0023 0.0003 0.0006 0.0001 0.0044 0.0026 0.0072 0.0012 0.0029 0.0004 0.0012 0.0031 0.0024 0.0002 0.0012 0.0002
Cell Periphery 0.0014 0.0005 0.0007 0 0.0005 0 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0306 0.071 0.046 0.0535 0.0986 0.074 0.069 0.0603 0.0669 0.0522 0.0462 0.0969 0.0662 0.114 0.1497 0.12 0.0555 0.2205
Cytoplasmic Foci 0.0537 0.0093 0.0143 0.0072 0.0024 0.0262 0.0131 0.0021 0.0066 0.0114 0.0212 0.01 0.0148 0.008 0.0115 0.0047 0.0127 0.0103
Eisosomes 0.0023 0.0004 0.0009 0.0001 0.0001 0.0003 0.0004 0 0.0002 0.0003 0.0002 0.0001 0.0006 0.0001 0.0002 0.0002 0.0006 0.0001
Endoplasmic Reticulum 0.0082 0.0022 0.0044 0.0008 0.003 0.0011 0.0038 0.0012 0.0014 0.0011 0.0034 0.0031 0.0028 0.0021 0.0022 0.0064 0.0008 0.003
Endosome 0.034 0.0035 0.0129 0.0555 0.0023 0.0059 0.015 0.0011 0.0037 0.0022 0.0414 0.0076 0.0067 0.0018 0.0033 0.0076 0.0029 0.0031
Golgi 0.0105 0.0015 0.0073 0.0027 0.0001 0.0045 0.007 0.0001 0.0011 0.001 0.0066 0.0017 0.002 0.0002 0.001 0.0026 0.0032 0.0001
Lipid Particles 0.0337 0.0041 0.0061 0.0021 0.0003 0.0076 0.013 0.0002 0.0012 0.001 0.0057 0.0076 0.0036 0.0005 0.0011 0.0012 0.0078 0.0003
Mitochondria 0.0256 0.0012 0.0122 0.0006 0.0014 0.0006 0.0124 0.0004 0.0194 0.0032 0.0019 0.0023 0.0022 0.0006 0.0015 0.0007 0.0121 0.0009
None 0.638 0.8643 0.8282 0.8661 0.8223 0.8649 0.5717 0.6188 0.6394 0.6876 0.5979 0.4826 0.7343 0.7855 0.7015 0.6655 0.6213 0.5667
Nuclear Periphery 0.0165 0.0019 0.0091 0.0009 0.0222 0.0008 0.0104 0.0047 0.004 0.0279 0.0098 0.0069 0.0075 0.0032 0.007 0.0084 0.0019 0.0037
Nucleolus 0.002 0.0005 0.0005 0.0002 0.0004 0.0003 0.003 0.0038 0.0039 0.0023 0.0053 0.0107 0.0042 0.0025 0.0029 0.0006 0.0008 0.0017
Nucleus 0.0133 0.0232 0.0307 0.0016 0.0293 0.0021 0.1716 0.2759 0.206 0.0972 0.1399 0.3066 0.081 0.0593 0.0844 0.1335 0.225 0.1698
Peroxisomes 0.0269 0.0017 0.0055 0.0003 0.0002 0.0065 0.0073 0.0001 0.0034 0.0048 0.0058 0.0034 0.0082 0.0004 0.0011 0.0003 0.0092 0.0002
Punctate Nuclear 0.0108 0.0035 0.0035 0.0009 0.0021 0.0035 0.0223 0.0219 0.0193 0.0511 0.0469 0.0477 0.0275 0.0122 0.0128 0.0048 0.0047 0.0128
Vacuole 0.0069 0.0008 0.0013 0.0055 0.0019 0.0003 0.0045 0.0023 0.0017 0.0008 0.0054 0.0028 0.002 0.002 0.0018 0.001 0.0004 0.0017
Vacuole Periphery 0.0034 0.0002 0.001 0.0011 0.0007 0.0001 0.0009 0.0003 0.0005 0.0006 0.0012 0.0006 0.0005 0.0003 0.0005 0.0005 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.5676 2.0296 0.753 1.8103 1.4718 5.6728 3.0782 3.4626 4.0667 3.2289
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Transcriptional repressor; binds promoter sequences of cyclin genes, CYS3, and SMF2; not expressed during log phase of growth, but induced by stress or starvation during mitosis, and late in meiosis; represses 15% of all yeast genes as cells transition to quiescence; important for maintaining G1 arrest and for longevity of quiescent cells; member of Swi4p/Mbp1p family; phosphorylated by Cdc28p; relative distribution to nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Xbp1

Xbp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Xbp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available