Standard name | |
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Human Ortholog | |
Description | 6-phosphofructo-2-kinase; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 2 | 2 | 1 | 4 |
Bud | 0 | 2 | 0 | 1 | 0 | 4 | 3 | 2 | 7 | – | 8 | 9 | 1 | 0 | 1 | 0 | 0 | – | 1 | 2 | 1 | 1 | 1 | 13 |
Bud Neck | 0 | 0 | 5 | 1 | 0 | 1 | 2 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 5 | 1 | 1 | 3 | 1 | 9 | 4 | – | 5 | 6 | 0 | 0 | 3 | 0 | 1 | – | 0 | 1 | 3 | 2 | 1 | 1 |
Cytoplasm | 195 | 439 | 253 | 129 | 299 | 354 | 418 | 398 | 313 | – | 228 | 249 | 241 | 176 | 304 | 43 | 47 | – | 184 | 400 | 195 | 141 | 230 | 245 |
Endoplasmic Reticulum | 4 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 1 | 0 | 6 | 5 | – | 0 | 2 | 1 | 0 | 3 | 6 |
Endosome | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | – | 3 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 9 | 0 | 0 | 1 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4 | 2 | – | 1 | 2 | 1 | 1 | 1 | 2 |
Mitochondria | 3 | 10 | 0 | 1 | 4 | 29 | 55 | 32 | 94 | – | 108 | 96 | 2 | 0 | 0 | 1 | 1 | – | 5 | 15 | 9 | 12 | 22 | 13 |
Nucleus | 1 | 0 | 1 | 2 | 4 | 4 | 8 | 11 | 4 | – | 10 | 7 | 0 | 0 | 0 | 2 | 2 | – | 0 | 2 | 3 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 2 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 4 | 2 | – | 2 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 6 | 2 | 0 | 1 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 0 | 2 | 4 | 5 | 2 | – | 1 | 5 | 0 | 1 | 1 | 1 | 1 | – | 0 | 6 | 5 | 8 | 9 | 15 |
Unique Cell Count | 201 | 452 | 254 | 130 | 303 | 367 | 449 | 422 | 355 | 296 | 316 | 243 | 176 | 306 | 52 | 51 | 204 | 462 | 236 | 176 | 282 | 324 | ||
Labelled Cell Count | 207 | 461 | 266 | 136 | 310 | 399 | 492 | 462 | 431 | 366 | 382 | 246 | 178 | 310 | 60 | 60 | 204 | 462 | 236 | 176 | 282 | 324 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.1 | 4.8 | 5.1 | 5.4 | 4.3 | 4.6 | 5.2 | 4.4 | – | 4.2 | 4.3 | 4.1 | 4.6 | 4.8 | 7.1 | 7.3 | – | 3.9 | 4.1 | 4.8 |
Std Deviation (1e-4) | 0.5 | 0.9 | 1.5 | 2.5 | 1.9 | 1.5 | 1.4 | 1.6 | 1.7 | – | 1.6 | 1.2 | 1.3 | 1.1 | 1.6 | 1.8 | 2.2 | – | 1.2 | 1.6 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.17 | -0.14 | -0.05 | 0.11 | -0.13 | – | -0.2 | -0.14 | -0.21 | -0.07 | 0.0 | 0.56 | 0.6 | – | -0.31 | -0.22 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2879 | 1.9773 | 1.5699 | 1.7293 | 1.3183 | 1.8572 | 0.8775 | 0.3306 | 0.0145 | 0.1535 | 0.2769 | -0.1733 | -0.9379 | -0.4665 | -0.8777 | -0.4697 | 0.8217 | -0.5409 |
Actin | 0.0266 | 0.0001 | 0.005 | 0.0115 | 0.0165 | 0.0081 | 0.0711 | 0.0084 | 0.014 | 0.0095 | 0.0179 | 0.0064 | 0.0331 | 0 | 0.0009 | 0 | 0.1243 | 0.0082 |
Bud | 0.0004 | 0.0008 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0067 | 0.0021 | 0.0045 | 0.0015 | 0.0024 | 0.0034 | 0.0074 | 0 | 0.0034 | 0 | 0.0006 | 0.0006 |
Bud Neck | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0005 | 0.0083 | 0.0004 | 0.0031 | 0.0006 | 0.0015 | 0.0022 | 0.0024 | 0 | 0.0004 | 0 | 0.0004 | 0.0006 |
Bud Periphery | 0.0006 | 0.0032 | 0.0003 | 0.0001 | 0.0007 | 0.0027 | 0.0224 | 0.0041 | 0.0067 | 0.002 | 0.0035 | 0.0088 | 0.0025 | 0 | 0.0088 | 0 | 0.0007 | 0.0036 |
Bud Site | 0.005 | 0.0009 | 0.0007 | 0.0002 | 0.0008 | 0.0001 | 0.0217 | 0.0088 | 0.012 | 0.0021 | 0.007 | 0.0004 | 0.0043 | 0.0001 | 0.0027 | 0 | 0.0013 | 0.0001 |
Cell Periphery | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0007 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.1193 | 0.1954 | 0.1571 | 0.259 | 0.0648 | 0.2266 | 0.1998 | 0.3403 | 0.3005 | 0.2735 | 0.3517 | 0.3889 | 0.0675 | 0.1647 | 0.1987 | 0.2394 | 0.1553 | 0.2029 |
Cytoplasmic Foci | 0.0081 | 0.0024 | 0.0143 | 0.0223 | 0.0167 | 0.0048 | 0.0423 | 0.0094 | 0.0262 | 0.025 | 0.0399 | 0.0285 | 0.0317 | 0.0014 | 0.0031 | 0.0047 | 0.0067 | 0.0022 |
Eisosomes | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0016 | 0.0007 | 0.0012 | 0.001 | 0.0008 | 0.001 | 0.0159 | 0.0018 | 0.0049 | 0.0013 | 0.0058 | 0.0019 | 0.0019 | 0.0005 | 0.0007 | 0.0013 | 0.0008 | 0.0006 |
Endosome | 0.0051 | 0.0004 | 0.004 | 0.0023 | 0.0143 | 0.0027 | 0.0483 | 0.0048 | 0.0159 | 0.0237 | 0.0338 | 0.0063 | 0.0179 | 0.0002 | 0.0011 | 0.002 | 0.0088 | 0.0007 |
Golgi | 0.0037 | 0.0001 | 0.0013 | 0.0013 | 0.0064 | 0.0013 | 0.0124 | 0.0006 | 0.0046 | 0.0228 | 0.0229 | 0.0009 | 0.0101 | 0 | 0.0001 | 0.0001 | 0.0261 | 0.0001 |
Lipid Particles | 0.0057 | 0.0001 | 0.0035 | 0.0051 | 0.0097 | 0.0033 | 0.0177 | 0.0002 | 0.0025 | 0.0256 | 0.0065 | 0.0008 | 0.0213 | 0.0001 | 0.0002 | 0.0002 | 0.0059 | 0 |
Mitochondria | 0.0083 | 0.0002 | 0.0021 | 0.001 | 0.0091 | 0.0082 | 0.0106 | 0.0005 | 0.0075 | 0.014 | 0.0265 | 0.0012 | 0.0048 | 0 | 0.0003 | 0.0001 | 0.0213 | 0.0001 |
None | 0.793 | 0.7928 | 0.7934 | 0.6684 | 0.822 | 0.7366 | 0.4491 | 0.6117 | 0.5811 | 0.5533 | 0.4254 | 0.5372 | 0.7427 | 0.8321 | 0.7742 | 0.7485 | 0.6422 | 0.7792 |
Nuclear Periphery | 0.0007 | 0.0001 | 0.0037 | 0.0004 | 0.003 | 0.0003 | 0.0164 | 0.0005 | 0.0041 | 0.0011 | 0.0092 | 0.0034 | 0.0018 | 0.0001 | 0.0003 | 0.0007 | 0.0006 | 0.0002 |
Nucleolus | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0015 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0018 | 0 | 0.0009 | 0 | 0.0001 | 0 |
Nucleus | 0.0008 | 0.0006 | 0.0009 | 0.0007 | 0.0007 | 0.0004 | 0.0037 | 0.0012 | 0.0022 | 0.0009 | 0.009 | 0.0023 | 0.0026 | 0.0002 | 0.0007 | 0.0006 | 0.0002 | 0.0003 |
Peroxisomes | 0.0105 | 0 | 0.0083 | 0.0248 | 0.0269 | 0.0017 | 0.0214 | 0.0001 | 0.0019 | 0.0385 | 0.0172 | 0.0025 | 0.0342 | 0 | 0.0002 | 0 | 0.0029 | 0 |
Punctate Nuclear | 0.0069 | 0.0002 | 0.0021 | 0.0008 | 0.0052 | 0.0004 | 0.0082 | 0.0007 | 0.0028 | 0.0013 | 0.0111 | 0.0024 | 0.0093 | 0.0001 | 0.0022 | 0.0005 | 0.0005 | 0.0001 |
Vacuole | 0.0007 | 0.0011 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0143 | 0.003 | 0.0032 | 0.0017 | 0.0052 | 0.0016 | 0.0014 | 0.0002 | 0.0007 | 0.0015 | 0.0004 | 0.0003 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0065 | 0.0003 | 0.001 | 0.001 | 0.0025 | 0.0006 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.3283 | 7.9418 | 8.2505 | 8.9992 | 6.0473 | 11.9869 | 12.1453 | 15.8526 | 14.4988 | 13.414 |
Translational Efficiency | 0.9324 | 0.906 | 0.6458 | 0.7909 | 0.9903 | 1.2182 | 0.8521 | 0.6697 | 0.8704 | 0.706 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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