Standard name
Human Ortholog
Description Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.42 0.35 0.51 0.47 0.45 0.45 0.36 0.21 0.35 0.42 0.42 0.3 0.35 0.3 0.61 0.49 0.38 0.42 0.27 0.67 0.54 0.52 0.47
Bud 0 0.13 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.1 0.14 0.07 0.07 0.05 0 0 0 0.05 0.08 0.1 0.11 0.06 0 0.05 0 0 0 0 0.05 0
Cytoplasm 0 0.19 0 0.06 0 0 0 0 0 0 0 0.09 0.06 0.09 0.14 0.16 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0.76 0.61 0.68 0.63 0.61 0.65 0.63 0.51 0.47 0.41 0.35 0.77 0.74 0.69 0 0.05 0 0.42 0.5 0.17 0.24 0.26 0.27
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.61 0.1 0.62 0.58 0.69 0.48 0.61 0.57 0.56 0.49 0.46 0.25 0.28 0.33 0.61 0.64 0.65 0.14 0.15 0.12 0.16 0.13 0.18
Mitochondria 0 0.05 0.05 0.11 0.16 0.36 0.23 0.25 0.44 0.58 0.52 0 0 0 0.1 0.14 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 115 45 282 205 278 285 365 199 373 179 368 108 186 114 125 213 108 116 39 372 163 355 369
Bud 7 16 14 6 22 19 31 31 48 21 54 9 10 7 14 18 16 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 8 6 54 59 43 47 50 24 45 12 46 30 52 40 12 9 15 1 1 11 6 30 30
Cytoplasm 8 24 22 24 20 23 23 15 13 10 11 33 34 35 28 70 59 1 3 1 2 4 5
Endoplasmic Reticulum 211 78 375 274 379 414 640 489 502 173 307 277 394 261 3 22 11 115 72 92 73 175 211
Endosome 0 0 1 0 2 0 3 1 4 0 4 0 0 1 1 6 3 0 1 1 0 1 2
Golgi 167 13 340 252 432 304 617 546 598 205 404 92 150 126 125 280 183 37 21 68 49 88 144
Mitochondria 11 6 30 48 97 226 232 241 468 243 451 0 0 0 20 63 32 0 1 3 4 10 3
Nucleus 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 4 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 1 2 5 18 18 7 10 1 0 0 0 2 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 3 7 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 0 2 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 0 1 2 5 3 2 4 1 3 1 0 1 2 0 1 0 0 1 0
Unique Cell Count 276 127 548 432 624 634 1011 952 1069 422 874 362 529 378 205 439 282 276 143 557 304 677 781
Labelled Cell Count 528 188 1119 869 1274 1321 1972 1577 2075 853 1661 551 829 585 328 689 429 276 143 557 304 677 781


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.3 20.2 21.7 22.2 21.3 19.1 19.2 19.2 18.6 18.3 18.2 25.2 25.1 26.0 16.4 17.9 21.8 24.8 25.1 27.5
Std Deviation (1e-4) 4.0 3.4 3.5 3.7 3.6 3.3 3.5 4.0 4.0 4.3 4.2 4.8 4.5 5.7 5.4 6.2 8.5 3.7 4.4 5.3
Intensity Change (Log2) 0.03 -0.03 -0.18 -0.18 -0.18 -0.22 -0.24 -0.25 0.22 0.21 0.26 -0.4 -0.27 0.01 0.19 0.21 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.2 -2.4 -2.2 -5.9 -12.2 -6.4 -2.8 -3.4 -6.5 -5.4 -6.4 2.3 -0.9 -3.6
Bud -1.3 1.0 0.5 0.6 0.8 1.9 2.0 3.1 -0.1 -0.7 -0.7 2.8 1.4 2.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.9 -1.8 -1.5 -3.7 -6.2 -4.5 -4.3 -3.3 -0.8 0 0.4 -1.7 -5.0 -2.2
Cytoplasm 1.1 -0.7 -0.3 -2.0 -2.9 -3.7 -1.4 -3.4 3.2 1.8 3.3 4.7 6.4 7.8
Endoplasmic Reticulum -1.6 -2.7 -1.1 -2.0 -6.4 -8.2 -8.5 -12.2 2.6 2.2 0.2 -16.4 -20.2 -17.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.2 2.6 -4.9 -0.4 -1.8 -2.4 -4.2 -5.8 -10.8 -11.1 -8.6 -0.3 0.6 0.8
Mitochondria 3.2 5.5 12.6 8.8 9.6 15.8 17.9 17.9 -4.5 -5.5 -4.6 2.1 4.7 3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.2 3.1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.7559 27.3253 25.7326 26.2441 24.8533 25.2174 18.7696 27.0943 24.5067 24.5484 23.2121 24.5331 22.1749 25.9854 23.288 24.8025 23.1423 23.6643
Actin 0.0704 0.0423 0.0393 0.0096 0.0444 0.0292 0.0827 0.0964 0.1331 0.1146 0.0669 0.043 0.1254 0.0764 0.1332 0.0351 0.049 0.0539
Bud 0.0001 0.0001 0.0001 0 0.0003 0 0.0005 0.0001 0.0002 0.0002 0.0002 0.0001 0.0004 0.0006 0.0004 0.0001 0 0.0001
Bud Neck 0.001 0.0006 0.0003 0.0003 0.0004 0.001 0.0012 0.0002 0.0008 0.0012 0.0005 0.0005 0.0007 0.0004 0.0017 0.0004 0.0002 0.0011
Bud Periphery 0.0002 0.0001 0.0002 0 0.0003 0.0001 0.0005 0.0001 0.0003 0.0005 0.0003 0.0001 0.0006 0.0004 0.0009 0.0002 0 0.0002
Bud Site 0.0004 0.0022 0.0007 0 0.0003 0 0.0059 0.0001 0.0002 0.0002 0.0001 0 0.0023 0.0016 0.0067 0.0001 0 0
Cell Periphery 0.0007 0.0012 0.0005 0.0006 0.0002 0.0005 0.0004 0.0002 0.0005 0.0009 0.0004 0.0003 0.0005 0.0019 0.0018 0.0008 0.0002 0.0003
Cytoplasm 0.003 0.0149 0.0013 0.0003 0.0016 0.0038 0.0017 0.0008 0.001 0.0007 0.0007 0.0006 0.0033 0.0025 0.0017 0.0006 0.0005 0.001
Cytoplasmic Foci 0.01 0.0107 0.0073 0.0028 0.0076 0.005 0.0242 0.009 0.0038 0.0033 0.0025 0.0029 0.0063 0.0107 0.0113 0.0043 0.0056 0.0039
Eisosomes 0.0034 0.0049 0.0016 0.0051 0.0018 0.0048 0.0026 0.0007 0.003 0.0052 0.0026 0.0008 0.0022 0.0013 0.0089 0.0045 0.0018 0.0006
Endoplasmic Reticulum 0.0097 0.016 0.0103 0.0084 0.0068 0.0196 0.0031 0.0018 0.0087 0.0113 0.0092 0.0084 0.0174 0.0189 0.0091 0.01 0.0048 0.0148
Endosome 0.0299 0.0208 0.0172 0.0122 0.0226 0.0199 0.035 0.0126 0.0135 0.0188 0.0221 0.012 0.024 0.0225 0.0277 0.0283 0.0173 0.0279
Golgi 0.8408 0.8802 0.9136 0.9563 0.8937 0.9121 0.7652 0.876 0.8135 0.8341 0.8879 0.915 0.7836 0.8338 0.7707 0.8648 0.9166 0.8925
Lipid Particles 0.0115 0.0046 0.0041 0.0031 0.0035 0.0023 0.0341 0.0014 0.0026 0.0041 0.001 0.0041 0.0119 0.0118 0.0099 0.0449 0.0026 0.0008
Mitochondria 0.0044 0.0003 0.0013 0.0004 0.0057 0.0007 0.0038 0.0003 0.0035 0.0029 0.0045 0.0018 0.0061 0.014 0.0061 0.0039 0.0006 0.0015
None 0.0015 0.0001 0.0003 0 0.0007 0 0.0021 0 0.0011 0.0001 0.0001 0.0001 0.0007 0.0004 0.0011 0.0001 0 0
Nuclear Periphery 0.0054 0.0003 0.0003 0.0004 0.0049 0.0004 0.0112 0.0001 0.0094 0.0006 0.0002 0.0074 0.01 0.0008 0.0033 0.0005 0.0001 0.0004
Nucleolus 0.0002 0 0 0 0.0001 0 0.001 0 0.0001 0 0 0 0.0001 0.0002 0.0002 0 0 0
Nucleus 0.0005 0.0001 0 0 0.0009 0 0.0042 0 0.0019 0 0 0.0001 0.0006 0.0001 0.0005 0 0 0
Peroxisomes 0.0011 0.0001 0.0009 0 0.0009 0 0.0076 0.0001 0.0016 0 0.0001 0.0001 0.0013 0.001 0.001 0.0001 0 0
Punctate Nuclear 0.0033 0.0001 0.0001 0 0.0024 0 0.0106 0 0.0004 0 0 0.0001 0.0003 0.0001 0.0019 0 0 0
Vacuole 0.0008 0.0002 0.0002 0.0001 0.0003 0.0001 0.001 0 0.0002 0.0003 0.0002 0.0002 0.0007 0.0003 0.0007 0.0004 0.0001 0.0003
Vacuole Periphery 0.0017 0.0002 0.0004 0.0004 0.0006 0.0003 0.0014 0.0001 0.0006 0.0008 0.0006 0.0026 0.0017 0.0004 0.0012 0.001 0.0003 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 106.3331 122.0113 101.8548 115.5104 98.2549 91.6021 113.7724 111.2196 100.95 114.0765
Translational Efficiency 1.4114 1.2844 1.4044 1.0812 1.3486 1.6687 1.3094 1.2698 1.3236 1.1835

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication
Localization
Cell Percentages ER (82%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Sec24

Sec24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available