Standard name
Human Ortholog
Description Histidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud – 0 0.07 0 0 0 0 0 0.05 0 0 0.08 0 0 0 – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 0 0 0 0.12 0.08 0 0 0.07 0.08 0 0 0 0.07 – – – 0 0 0 0 0 0
Cytoplasm – 1.0 1.0 1.0 0.91 0.92 0.95 0.89 0.84 0.86 0.88 0.81 0.98 0.99 0.97 – – – 0.8 0.97 0.91 0.81 0.88 0.92
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0.1 0.12 0.06 0 0 0 0 – – – 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.07 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0.05 0 0 0.08 0 0 0.08 0.11 0 0 0 – – – 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 2 1 0 1 0 0 0 – – – 0 0 0 0 0 0
Bud – 0 5 0 1 1 1 3 4 1 0 3 2 0 2 – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 2 1 0 2 8 11 4 1 8 5 1 0 0 7 – – – 0 0 0 0 0 0
Cytoplasm – 109 67 22 74 59 123 85 67 94 58 29 88 70 93 – – – 3 107 76 21 48 63
Endoplasmic Reticulum – 0 0 0 0 1 2 3 8 13 4 0 0 1 0 – – – 0 0 2 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0 0 1 0 0 0 1 0 0 0 1 0 0 – – – 0 0 0 0 0 0
Nucleus – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – 0 1 1 2 0 1
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 4 0 2 8 2 1 5 4 1 0 0 – – – 0 0 2 1 2 1
Unique Cell Count 109 67 22 81 64 130 95 80 109 66 36 90 71 96 5 111 84 27 55 69
Labelled Cell Count 111 73 22 83 69 139 103 85 118 72 38 92 71 102 5 111 84 27 55 69


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 56.5 62.6 48.8 39.7 39.7 35.6 32.4 29.3 29.5 27.1 27.8 62.7 76.0 68.1 – – – – 79.8 79.6
Std Deviation (1e-4) – 11.6 27.8 16.1 14.6 14.4 10.9 9.3 9.9 7.6 8.5 8.8 21.6 19.0 19.4 – – – – 25.8 22.8
Intensity Change (Log2) – – – -0.36 -0.66 -0.66 -0.81 -0.95 -1.1 -1.08 -1.21 -1.17 0.0 0.28 0.12 – – – – 0.35 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 -2.5 -2.3 -1.9 -2.7 -3.5 -3.2 -2.9 -3.7 -1.2 -1.0 -1.5 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0902 0.0004 0.0091 0 0.0068 0.0004 – – – – – –
Bud – – – – – – 0.0005 0.0004 0.0012 0 0.013 0.0002 – – – – – –
Bud Neck – – – – – – 0.0006 0.0002 0.0002 0.0001 0.001 0.0009 – – – – – –
Bud Periphery – – – – – – 0.0005 0.0002 0.0014 0 0.0088 0.0001 – – – – – –
Bud Site – – – – – – 0.0023 0.0007 0.0012 0 0.0014 0 – – – – – –
Cell Periphery – – – – – – 0.0001 0.0001 0.0001 0 0.0004 0 – – – – – –
Cytoplasm – – – – – – 0.0113 0.9904 0.9518 0.9973 0.2753 0.8557 – – – – – –
Cytoplasmic Foci – – – – – – 0.294 0.0008 0.0014 0.0001 0.0287 0.0035 – – – – – –
Eisosomes – – – – – – 0.0005 0 0.0001 0 0 0 – – – – – –
Endoplasmic Reticulum – – – – – – 0.0052 0.0004 0.0035 0 0.0096 0.0006 – – – – – –
Endosome – – – – – – 0.1077 0.0008 0.0033 0 0.1934 0.0387 – – – – – –
Golgi – – – – – – 0.0686 0.0001 0.0006 0 0.0183 0.0233 – – – – – –
Lipid Particles – – – – – – 0.1343 0 0.0002 0 0.0007 0.0006 – – – – – –
Mitochondria – – – – – – 0.0113 0.0005 0.01 0 0.0739 0.0667 – – – – – –
None – – – – – – 0.013 0.0003 0.0008 0 0.0028 0.0002 – – – – – –
Nuclear Periphery – – – – – – 0.0078 0.0007 0.0081 0.0002 0.0208 0.0012 – – – – – –
Nucleolus – – – – – – 0.0005 0.0001 0.0001 0 0.0005 0 – – – – – –
Nucleus – – – – – – 0.001 0.0028 0.0045 0.002 0.006 0.0057 – – – – – –
Peroxisomes – – – – – – 0.2089 0 0.0003 0 0.002 0 – – – – – –
Punctate Nuclear – – – – – – 0.0398 0.0001 0.0004 0 0.0004 0.0001 – – – – – –
Vacuole – – – – – – 0.0007 0.001 0.001 0.0001 0.1489 0.0012 – – – – – –
Vacuole Periphery – – – – – – 0.0012 0.0002 0.0006 0 0.1874 0.0007 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.8784 23.8502 28.443 32.4445 31.5031 50.4733 43.2777 50.8623 48.0929 49.9492
Translational Efficiency 1.3263 1.6873 1.0807 1.0114 1.0959 1.8767 1.3083 0.885 1.2884 1.0598

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Histidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

His5

His5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (His5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available