Standard name | |
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Human Ortholog | |
Description | ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; promotes base excision repair in chromatin; essential helicase-related protein homologous to Snf2p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 2 | – | – | – | 0 | 3 | 1 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 4 | 14 | 15 | 11 | 11 | – | 7 | 13 | 0 | 1 | 4 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 15 | 8 | 10 | 16 | 10 | 19 | 15 | 15 | – | 5 | 18 | 32 | 35 | 34 | – | – | – | 1 | 2 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 2 | 1 |
Endosome | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | – | – | – | 2 | 4 | 4 | 0 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 2 | 1 | 2 | 0 |
Mitochondria | 0 | 4 | 17 | 14 | 6 | 26 | 24 | 49 | 38 | – | 21 | 29 | 3 | 5 | 6 | – | – | – | 1 | 1 | 1 | 0 | 2 | 4 |
Nucleus | 274 | 350 | 285 | 213 | 198 | 254 | 280 | 256 | 188 | – | 138 | 160 | 273 | 373 | 334 | – | – | – | 249 | 366 | 271 | 240 | 185 | 252 |
Nuclear Periphery | 2 | 0 | 3 | 0 | 1 | 4 | 1 | 4 | 5 | – | 2 | 5 | 1 | 2 | 1 | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 113 | 139 | 135 | 86 | 83 | 145 | 181 | 187 | 158 | – | 138 | 159 | 127 | 157 | 181 | – | – | – | 93 | 54 | 79 | 93 | 148 | 161 |
Peroxisomes | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 5 | 5 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 4 | 1 | – | 0 | 0 | 3 | 17 | 22 | – | – | – | 3 | 3 | 0 | 2 | 2 | 5 |
Vac/Vac Membrane | 0 | 3 | 2 | 3 | 2 | 0 | 1 | 2 | 1 | – | 0 | 1 | 1 | 4 | 3 | – | – | – | 4 | 11 | 8 | 9 | 8 | 21 |
Unique Cell Count | 325 | 419 | 349 | 257 | 257 | 341 | 407 | 402 | 302 | 221 | 267 | 346 | 491 | 461 | 361 | 452 | 373 | 354 | 367 | 461 | ||||
Labelled Cell Count | 396 | 515 | 453 | 327 | 312 | 457 | 526 | 529 | 418 | 312 | 388 | 441 | 601 | 594 | 361 | 452 | 373 | 354 | 367 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.6 | 13.1 | 8.7 | 8.7 | 9.0 | 7.8 | 7.9 | 7.1 | 7.1 | – | 6.8 | 6.6 | 11.9 | 10.9 | 9.9 | – | – | – | 11.6 | 12.2 | 12.5 |
Std Deviation (1e-4) | 4.2 | 3.7 | 2.5 | 3.2 | 2.7 | 2.0 | 2.3 | 2.1 | 2.1 | – | 1.9 | 1.9 | 3.7 | 3.6 | 3.5 | – | – | – | 2.9 | 3.1 | 2.9 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.06 | -0.15 | -0.14 | -0.28 | -0.29 | – | -0.36 | -0.4 | 0.46 | 0.32 | 0.18 | – | – | – | 0.41 | 0.49 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 3.4 | 3.2 | 2.7 | 3.2 | – | 0 | 3.8 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 1.1 | 2.5 | 0.5 | 1.8 | 1.1 | 1.8 | – | 0 | 2.7 | 3.9 | 3.1 | 3.2 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0.3 | -1.6 | 1.5 | 0.6 | 3.5 | 3.5 | – | 2.2 | 2.8 | -3.2 | -3.4 | -3.0 | – | – | – |
Nucleus | 0.4 | -1.4 | -2.3 | -4.1 | -5.5 | -5.5 | – | -5.1 | -6.0 | -0.9 | -2.0 | -3.1 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | -1.3 | -1.6 | 1.0 | 1.6 | 2.2 | 3.5 | – | 5.5 | 5.1 | -0.5 | -2.0 | 0.2 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.1 | 3.8 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.8224 | 14.5592 | 14.4941 | 13.8944 | 14.136 | 13.8048 | 9.4329 | 7.9412 | 8.3391 | 7.801 | 7.0875 | 7.7222 | 15.1274 | 14.2401 | 14.3242 | 14.3139 | 13.1046 | 13.504 |
Actin | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0 | 0.0048 | 0 | 0.0006 | 0 | 0.0395 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 |
Bud Periphery | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.001 | 0 | 0.0034 | 0 | 0.0002 | 0 | 0.0003 | 0 |
Cytoplasmic Foci | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0.0044 | 0 | 0.0069 | 0 | 0.0029 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0.0007 | 0 | 0 | 0 | 0.0004 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0062 | 0 | 0.0029 | 0 | 0.0016 | 0 | 0.0038 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0007 | 0 | 0.0024 | 0 | 0.0041 | 0 |
Lipid Particles | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0032 | 0 | 0.001 | 0 | 0.0033 | 0 | 0.013 | 0 |
Mitochondria | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0006 | 0.0001 | 0 | 0 | 0.001 | 0 | 0.0017 | 0 | 0.0006 | 0 | 0.0014 | 0.0001 |
None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.009 | 0 | 0.0096 | 0 | 0.0003 | 0 | 0.0033 | 0 |
Nuclear Periphery | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0116 | 0 | 0.0099 | 0.0002 | 0.0002 | 0.0001 | 0.0019 | 0 |
Nucleolus | 0.0978 | 0.0491 | 0.0439 | 0.0334 | 0.3758 | 0.2246 | 0.087 | 0.043 | 0.031 | 0.0242 | 0.3155 | 0.175 | 0.0847 | 0.052 | 0.0437 | 0.0276 | 0.4183 | 0.2262 |
Nucleus | 0.9017 | 0.9504 | 0.9557 | 0.9665 | 0.6226 | 0.7745 | 0.9106 | 0.9563 | 0.9688 | 0.9756 | 0.6411 | 0.8246 | 0.8702 | 0.9476 | 0.9286 | 0.9722 | 0.4942 | 0.7732 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0009 | 0 | 0.0015 | 0 | 0.0098 | 0 |
Punctate Nuclear | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0055 | 0.0001 | 0.0018 | 0.0001 | 0.0092 | 0 | 0.0051 | 0.0001 |
Vacuole | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0006 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Vacuole Periphery | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0 | 0.0008 | 0 | 0 | 0 | 0.0003 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.2927 | 49.077 | 41.6198 | 44.5118 | 42.7781 | 36.7598 | 49.4384 | 44.8751 | 43.6194 | 47.3326 |
Translational Efficiency | 0.8889 | 0.7558 | 0.7679 | 0.6709 | 0.6139 | 0.688 | 0.6312 | 0.5434 | 0.5526 | 0.5534 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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