Standard name
Human Ortholog
Description Zinc cluster protein proposed to be a transcriptional regulator; regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0.05 0 0 – 0 0 0.07 0.14 0.16 0.08 0.08 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.22 0.07 0.26 0.33 0.19 0.4 – 0.38 0.4 0 0 0.08 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.97 0.97 0.91 0.9 0.9 0.81 0.86 0.78 – 0.73 0.81 0.93 0.83 0.78 0.89 0.87 0.81 0.97 0.93 0.95 0.91 0.8 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0 0.09 0.12 0.12 0.16 – 0.19 0.23 0 0 0.05 0 0 0 0 0 0 0 0 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 0 0 1 1 3 1 – 1 0 0 0 0 0 0 0 0 1 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 1 0 0 0 1 3 3 13 11 – 3 5 0 1 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 2 3 10 8 14 12 11 – 7 7 16 41 48 33 17 29 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 – 0 0 0 0 1 4 5 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 2 0 2 2 1 1 0 0 0 1 5
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 1 0 0 0 2
Mitochondria 0 4 14 39 15 51 92 67 104 – 60 64 5 0 24 14 3 7 1 0 1 0 1 3
Nucleus 240 141 168 159 181 179 224 311 201 – 116 128 213 244 242 365 184 176 237 144 166 122 161 171
Nuclear Periphery 1 1 0 1 2 3 7 6 2 – 3 3 0 4 2 3 0 1 0 0 0 0 0 0
Nucleolus 2 1 1 11 2 18 33 43 40 – 31 36 3 5 16 2 0 4 0 0 0 0 6 25
Peroxisomes 1 0 0 0 0 0 0 0 0 – 0 0 0 2 2 1 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 1 1 – 0 0 0 2 3 0 0 1 1 0 0 0 1 4
Vac/Vac Membrane 1 0 2 2 5 0 1 1 1 – 1 1 2 2 8 10 5 11 1 5 5 4 19 48
Unique Cell Count 245 145 173 174 202 200 276 362 258 159 159 230 293 309 411 212 216 244 155 175 134 202 273
Labelled Cell Count 250 149 187 215 216 264 375 457 372 222 245 239 303 348 434 217 233 244 155 175 134 202 273


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 7.0 5.7 5.5 6.1 5.2 4.8 5.1 4.6 – 4.6 4.6 7.4 6.9 6.1 7.9 8.8 9.0 6.9 7.1 7.8
Std Deviation (1e-4) 1.6 1.4 0.8 1.1 1.8 1.4 1.2 1.3 1.2 – 1.1 1.1 1.8 1.8 1.8 1.5 2.0 2.3 1.4 1.5 1.4
Intensity Change (Log2) – – – -0.04 0.1 -0.12 -0.24 -0.16 -0.3 – -0.3 -0.31 0.38 0.28 0.1 0.47 0.64 0.66 0.29 0.32 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 2.1 0 2.2 0 1.8 – 0 0 2.8 4.6 5.0 3.2 3.1 4.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.7 -0.2 4.4 0 3.1 0 – 0 0 -2.8 -4.9 -0.1 -2.4 -3.2 -2.1
Nucleus -2.3 -2.9 -2.9 -4.9 -4.0 -5.6 – -6.3 -4.9 -2.0 -4.5 -5.6 -3.2 -3.6 -4.8
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 2.9 0 3.7 0 4.5 0 – 0 0 0 0 2.6 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6234 6.3094 6.0582 5.5402 5.5974 5.9248 6.5886 6.4589 6.4966 5.946 6.4066 6.2822 6.6714 6.2767 5.6332 5.8214 5.9821 6.2171
Actin 0.0367 0 0.0077 0.0006 0 0 0.0567 0.0001 0.023 0.0138 0.0014 0.0023 0.0394 0 0.017 0.009 0.0002 0.0001
Bud 0.0002 0 0 0.0001 0 0 0.0014 0 0.0001 0.0001 0.0001 0.0001 0.0006 0 0.0002 0.0001 0 0
Bud Neck 0.0023 0 0.0006 0.0017 0.0002 0.0003 0.0029 0 0.0002 0.001 0.0002 0.0004 0.0017 0 0.0006 0.0002 0.0002 0.0003
Bud Periphery 0.0002 0 0 0.0003 0.0001 0 0.0037 0 0.0002 0.0002 0.0001 0.0002 0.001 0 0.0002 0.0003 0.0001 0
Bud Site 0.0003 0 0.0001 0.0004 0.0001 0.0001 0.0074 0.0001 0.0003 0.0005 0.0002 0.0001 0.0023 0 0.0042 0.0013 0.0001 0
Cell Periphery 0.0001 0 0 0.0001 0 0 0.0009 0 0.0002 0.0001 0 0.0001 0.0006 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.0034 0 0.0002 0.0008 0 0 0.0164 0.0086 0.0054 0.0045 0.0001 0.0001 0.0049 0.0007 0.0017 0.0001 0.0034 0.0003
Cytoplasmic Foci 0.0016 0 0.0045 0.0004 0 0.0043 0.0401 0.0001 0.004 0.0007 0.0043 0.0003 0.0198 0 0.0122 0.0018 0.003 0.0005
Eisosomes 0.0003 0 0.0002 0 0 0 0.0006 0 0.0001 0.0001 0 0 0.0007 0 0.0001 0.0003 0 0.0001
Endoplasmic Reticulum 0.004 0 0.0009 0.0008 0 0 0.0136 0.0001 0.0051 0.0028 0.0001 0.0005 0.0092 0 0.0011 0.0002 0.0008 0.0001
Endosome 0.0112 0 0.0009 0.0021 0 0.0004 0.0441 0.0001 0.0022 0.0094 0.0127 0.0028 0.0239 0 0.0129 0.0008 0.0051 0.0006
Golgi 0.0048 0 0.0007 0.0002 0 0.0002 0.0131 0 0.0009 0.0023 0.0151 0.0019 0.0094 0 0.0038 0.0012 0.0018 0.0001
Lipid Particles 0.0026 0 0.0022 0.0003 0 0.0015 0.0299 0 0.0033 0.0017 0.0049 0.001 0.0112 0 0.0049 0.0088 0.0174 0.0017
Mitochondria 0.0012 0.0001 0.0004 0.001 0.0008 0.0001 0.0121 0.0001 0.0049 0.0019 0.0141 0.0026 0.0057 0.0001 0.0015 0.0064 0.0044 0.0003
None 0.0029 0 0.0005 0.0003 0 0.0001 0.016 0.0004 0.003 0.0013 0 0.0001 0.0044 0 0.0004 0.0003 0.0467 0.0058
Nuclear Periphery 0.0094 0.0002 0.0029 0.0085 0.0005 0.0001 0.042 0.0023 0.0055 0.0174 0.0002 0.009 0.0358 0.0026 0.0038 0.0006 0.006 0.0003
Nucleolus 0.0092 0.0099 0.0078 0.0197 0.0588 0.0283 0.0211 0.0124 0.0107 0.0198 0.0195 0.0188 0.0199 0.0073 0.0068 0.0042 0.0258 0.0259
Nucleus 0.9038 0.9896 0.9644 0.9553 0.9389 0.9595 0.6089 0.9703 0.922 0.9152 0.9245 0.9566 0.7557 0.9884 0.9146 0.9575 0.8783 0.9632
Peroxisomes 0.0008 0 0.0033 0.0001 0 0.0011 0.0123 0 0.0022 0.0003 0.0017 0.0001 0.0068 0 0.0077 0.0054 0.0004 0.0002
Punctate Nuclear 0.0044 0.0001 0.0025 0.0058 0.0001 0.0037 0.045 0.0051 0.0044 0.004 0.0001 0.0004 0.0342 0.0003 0.0035 0.001 0.0035 0.0003
Vacuole 0.0004 0 0.0001 0.001 0.0002 0.0001 0.0064 0.0002 0.0011 0.0018 0.0004 0.0008 0.0088 0.0003 0.0018 0.0001 0.0016 0.0002
Vacuole Periphery 0.0005 0 0.0001 0.0005 0.0002 0 0.0051 0.0001 0.0013 0.001 0.0003 0.0018 0.004 0.0002 0.0009 0.0002 0.0012 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4362 10.9029 14.4111 19.4838 18.2842 9.9042 16.1914 17.0897 13.4721 18.8652
Translational Efficiency 0.6554 1.0727 0.6911 0.4408 0.5973 1.0574 0.6317 0.6352 0.6961 0.5961

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zinc cluster protein proposed to be a transcriptional regulator; regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Asg1

Asg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available