Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.05 0.26 0.23 0.1 0.1 0.09 0.09 0.05 0.09 0 0.08 0.14 0.13 0.19 0.11 0.11 0.09 0.06 0 0.11 0.05 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.15 0 0 0 0.1 0.07 0.06 0.46 0.51 0.49 0.45 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.88 0.91 0.74 0.7 0.85 0.79 0.88 0.82 0.78 0.66 0.78 0.71 0.84 0.84 0.75 0.84 0.8 0.81 0.89 0.9 0.77 0.8 0.73 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0.05 0 0 0.05 0.05 0.11 0.09 0 0.05 0 0.05 0.14 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 1 0 0 0 2 2 2 1 0 0 0 1 0 0 0 2 1 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 1 4 5 8 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 14 8 10 17 15 21 20 10 14 8 18 20 30 53 16 13 17 3 2 6 7 14 7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 3 0 0 1 0 0 1
Endosome 0 0 0 0 0 0 1 1 0 0 0 0 0 1 2 0 1 1 0 1 1 1 0 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Mitochondria 2 46 0 1 1 15 16 13 89 76 124 97 1 0 1 7 3 4 0 3 0 2 1 6
Nucleus 14 274 23 31 141 121 215 174 152 98 197 152 121 200 206 125 96 145 42 282 48 111 195 164
Nuclear Periphery 0 1 0 1 0 1 0 1 4 0 2 2 2 0 1 5 2 1 0 0 0 1 0 4
Nucleolus 0 2 0 0 2 5 3 4 11 7 13 9 0 3 1 1 0 0 0 0 0 2 5 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 2 3 0 2 2 2 0 3 1 0 0 2 0 1 0 3 3 1
Vac/Vac Membrane 0 12 0 3 8 8 4 9 2 3 5 10 3 9 13 8 13 16 0 16 1 6 36 53
Unique Cell Count 16 301 31 44 166 153 244 211 195 149 251 214 144 239 274 149 120 179 48 313 63 140 267 266
Labelled Cell Count 17 350 32 46 170 166 264 226 274 207 361 298 147 246 279 167 128 189 48 313 63 140 267 266


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 5.7 8.2 7.0 7.3 5.6 5.5 5.8 4.5 4.1 4.3 4.4 7.1 7.0 6.8 6.6 7.8 8.9 7.8 8.2 7.8
Std Deviation (1e-4) 1.3 1.5 1.6 1.8 1.7 1.3 1.1 1.8 1.1 1.0 0.8 1.0 1.6 1.4 1.4 1.8 1.8 2.6 1.8 1.7 1.5
Intensity Change (Log2) – – – -0.23 -0.17 -0.56 -0.57 -0.51 -0.86 -1.01 -0.92 -0.9 -0.21 -0.23 -0.27 -0.33 -0.07 0.11 -0.08 -0.01 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 0 0 0 0 0 0 0 0 0 0 -0.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.4 1.5 0.6 2.1 1.1 0.5 -0.9 0.5 -0.4 1.3 1.3 0.1 1.3 0.7 0.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 91.1589 108.5362 104.1323 104.1411 103.2101 93.1533 81.5672 109.3743 98.7646 85.7965 75.6536 80.2624 87.6943 111.6192 102.1211 99.1069 90.37 79.977
Actin 0.0129 0.0007 0.0043 0.0005 0.0001 0.004 0.0127 0.0006 0.0092 0.0024 0.0187 0.0028 0.0018 0.0005 0.0041 0.0003 0.0001 0.0236
Bud 0.0021 0.0016 0.0049 0.0027 0.0008 0.0008 0.002 0.0006 0.0015 0.0035 0.0041 0.0054 0.0009 0.0007 0.0034 0.0015 0.0003 0.0008
Bud Neck 0.0008 0.0009 0.0015 0.0015 0.0007 0.0013 0.0008 0.0005 0.0007 0.0014 0.0019 0.0023 0.0008 0.0005 0.0007 0.0011 0.0003 0.0028
Bud Periphery 0.0024 0.0012 0.0038 0.001 0.0004 0.0025 0.0025 0.0005 0.0009 0.0025 0.007 0.003 0.0009 0.0004 0.0051 0.0007 0.0001 0.0005
Bud Site 0.001 0.0014 0.0012 0.0004 0.0001 0.0002 0.0016 0.0004 0.0009 0.0006 0.0008 0.0007 0.0011 0.0016 0.0023 0.0003 0.0001 0.0011
Cell Periphery 0.0232 0.0086 0.0153 0.0091 0.001 0.0059 0.0105 0.0054 0.0113 0.0133 0.0053 0.0034 0.018 0.0114 0.0063 0.0046 0.009 0.0013
Cytoplasm 0.5705 0.877 0.7221 0.6151 0.7084 0.6584 0.6308 0.9474 0.8533 0.5815 0.6101 0.6405 0.7367 0.9139 0.8254 0.661 0.671 0.801
Cytoplasmic Foci 0.0041 0.0021 0.0032 0.0028 0.0074 0.0101 0.0127 0.0012 0.0023 0.0044 0.0101 0.013 0.0066 0.0025 0.0027 0.0115 0.0221 0.0145
Eisosomes 0.0004 0 0.0002 0 0 0.0002 0.0004 0 0 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0004
Endoplasmic Reticulum 0.0803 0.0289 0.0447 0.004 0.0026 0.0979 0.0802 0.0131 0.0177 0.0294 0.0202 0.0559 0.0569 0.0073 0.0055 0.0019 0.0011 0.0083
Endosome 0.0185 0.0067 0.0128 0.0107 0.0657 0.0578 0.0126 0.003 0.0082 0.0198 0.0396 0.0433 0.0079 0.002 0.0048 0.0051 0.0103 0.0212
Golgi 0.0037 0.0009 0.0017 0.0003 0.0031 0.0067 0.0037 0.001 0.0016 0.0026 0.0089 0.0338 0.0016 0.0005 0.0006 0.0003 0.0004 0.0026
Lipid Particles 0.0017 0.0007 0.0013 0.0011 0.0005 0.004 0.0042 0.0004 0.0007 0.0024 0.0028 0.0011 0.0061 0.0011 0.0016 0.0042 0.0086 0.0027
Mitochondria 0.0118 0.001 0.005 0.0018 0.0006 0.0117 0.0162 0.0008 0.0024 0.0133 0.0481 0.0153 0.0028 0.0007 0.005 0.0032 0.0085 0.0012
None 0.0042 0.0002 0.0019 0.0011 0.0002 0.0309 0.0065 0.0002 0.0006 0.0019 0.0035 0.0031 0.0037 0.0002 0.002 0.0126 0.0074 0.0302
Nuclear Periphery 0.0552 0.0035 0.0112 0.0182 0.0032 0.0527 0.0397 0.0029 0.0162 0.0128 0.0482 0.0064 0.0299 0.0048 0.0094 0.0068 0.0061 0.0233
Nucleolus 0.0014 0.0001 0.0009 0.0034 0.0001 0.0004 0.0051 0.0001 0.0004 0.002 0.0155 0.0054 0.0057 0.0002 0.0006 0.0043 0.0773 0.0025
Nucleus 0.013 0.0019 0.005 0.0127 0.0014 0.0055 0.0162 0.0017 0.0051 0.0076 0.0144 0.0148 0.0117 0.0021 0.0054 0.0074 0.0109 0.0146
Peroxisomes 0.0003 0.0001 0.0002 0.0001 0.0001 0.0006 0.0015 0 0.0001 0.0003 0.0007 0.0005 0.01 0.0029 0.0004 0.0005 0.0007 0.0011
Punctate Nuclear 0.0014 0 0.0001 0.0002 0 0.0063 0.006 0 0.0002 0.0002 0.0014 0.0003 0.0018 0 0.0004 0.0012 0.0098 0.0313
Vacuole 0.1422 0.0514 0.1175 0.2417 0.1367 0.0191 0.1039 0.0169 0.054 0.22 0.0622 0.1034 0.0713 0.0414 0.0897 0.2154 0.0853 0.0121
Vacuole Periphery 0.049 0.0112 0.041 0.0715 0.067 0.0229 0.0301 0.0032 0.0126 0.0783 0.0763 0.0454 0.0239 0.0051 0.0242 0.0561 0.0705 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1010.5346 1132.6886 1527.3016 1280.031 1304.2414 979.6483 1087.9142 1010.8115 1095.0923 1087.2114
Translational Efficiency 1.5026 1.5803 1.2797 1.6221 1.4529 1.6372 1.5377 1.4764 1.4218 1.5709

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl16a

Rpl16a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl16a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available